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Tobacco floral homeotic gene homologues: partial sequence and expression pattern

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Chinese Science Bulletin 1998年第10期43卷 870-875页

作者： Jinsong Zhang Shouyi Chen Acad Sinica Inst Genet Beijing 100101 Peoples R China

Using PCR approach, three cDNA sequences, NTSQUA4, NTSQUA12 and NTSQUA15, were amplified from first_strand cDNAs of wild tobacco flower buds and identified as homologues for floral homeotic genes. All the three clones contained domains that a floral homeotic gene generally had, i.e. I domain, K domain and C_terminal domain except MADS_ box since the PCR primers were designed beyond this region. In addition, the amino acid sequences of them showed 50%-60% identity (70%-80% similarity) with the known floral organ identity class A gene AP1 and SQUA, possibly indicating that they are class A_like genes. NTSQUA4 and NTSQUA412 shared 95% identity in their amino acid sequence, while NTSQUA415 exhibited only 47% identity as compared with NTSQUA4 and NTSQUA12. Within tobacco flower, NTSQUA4 was expressed in sepals, petals and carpels, but not in stamens, while NTSQUA15 was expressed in every whorl of the flower. The possible functions of these genes are discussed.

关键词： nicotiana tabacum floral homeotic gene flower organ identity.

Molecular mapping of split rice spikelet mutant srs-1 and analysis of its homeotic function in rice

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Science China(Life Sciences) 2000年第4期43卷 369-375页

作者：白素兰刘永胜孙敬三朱立煌 Institute of Botany Chinese Academy of Sciences Beijing 100093 China College of Life Science Peking University Beijing 100871 China Institute of Botany Chinese Academy of Sciences Beijing 100093 China Institute of Genetics Chinese Academy of Sciences Beijing 100101 China

srs-1, a new floral organ identity gene in rice, was mapped using RAPD and RFLP markers. Firstly, the cross was made between 'ZhaiYeQing 8' (ZYQ8, indica) and split rice spikelet (SRS, japonica) mutant. The ratio of wild-type individuals and mutant plants in F2 population is 3:1, which indicates that the mutant characteristics are controlled by single recessive gene, srs-1. Consequently, BSA method was adopted and 520 random 10-mer primers were used to screen polymorphic bands between two bulks. Six primers could amplify polymorphic bands, of which S465 generates the most stable RAPD patterns. Then, S465 that cosegregates in F2 population has been converted into an RFLP marker successfully. Furthermore, srs-1 gene was mapped on chromosome 3 using DH mapping population. The effect of srs-1 gene results in the mutant of split rice spikelet. The mutant has longer and softer palea/lemma than those of wild-type, and two small palea/lemma-like organs between palea and lemma. In addition, there is a flower with three stamens and carpel in the axil of lemma. Thus, there are nine stamens and two carpels in the spikelet of mutant, srs-1 gene may belong to homeotic gene of class A according to the mutant characteristics and ABC model.

关键词： Oryza sativa RAPD RFLP srs-1 gene mapping homeotic gene.

Structural and functional analysis of a MADS box containing genomic DNA sequence cloned from rice

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Science China(Life Sciences) 1998年第6期41卷 561-568页

作者：孟征刘国振许智宏唐广王宁陈润生朱立煌 Institute of Genetics Chinese Academy of Sciences Beijing China Shanghai Institute of Plant Physiology Chinese Academy of Sciences Shanghai China Institute of Biophysics Chinese Academy of Sciences Beijing China

A putative MADS box gene (RgMADS1) was cloned by screening a rice genomic library with a heterogeneous MADS box probe derived from \%Antirrhinum majus squamosa\% gene. Sequencing and Southern analysis showed that RgMADS1 contains a highly conserved MADS box sequence, whereas the rice genome contains numbers of MADS box genes. A chimera gene of RgMADS1 under the control of CaMV 35S promoter was constructed and used to transform \%Arabidopsis.\% Some transformants exhibited abnormal phenotypes in their flowers, which were reflected in the floral structure, number and setting position. Therefore, RgMADS1 is likely a member of rice MADS box gene family and may be involved in the floral establishment and function control in the rice developmental process.

关键词： rice MADS box genes gene cloning homeotic gene.