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aBIOTECH 2024年第2期5卷 219-224页

作者： Yun Zhang Miao-Miao Cui Run-Nan Ke Yue-Dan Chen Kabin Xie national key {2. of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China Hubei key laboratory of Plant Pathology Huazhong Agricultural UniversityWuhan 430070China

Loss-of-function mutants are fundamental resources for gene function ***,it is difficult to generate viable and heritable knockout mutants for essential ***,we show that targeted editing of the C-terminal sequence of the embryo lethal gene MITOGEN-ACTIVATED PROTEIN KINASES 1(OsMPK1)results in weak *** C-terminal-edited osmpk1 mutants displayed severe developmental defects and altered disease resistance but generated tens of viable seeds that inherited the *** the same C-terminal editing approach,we also obtained viable mutants for a wallassociated protein kinase(Os07g04932.0)and a leucine-rich repeat receptor-like protein kinase(Os01g02.9700),while the null mutations of these genes were *** data suggest that protein kinase activity could be reduced by introducing frameshift mutations adjacent to the C-terminus,which could generate valuable resources for gene function studies and tune protein kinase activity for signaling pathway engineering.

关键词： CRISPR/Cas9 Lethal mutant Protein kinases Genome editing C-terminus

The genetic basis and process of inbreeding depression in an elite hybrid rice

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Science China(Life Sciences) 2024年第8期67卷 1727-1738页

作者： Xiaodong Xu Yawen Xu Jian Che Xu Han Zhengji Wang Xianmeng Wang Qinghua Zhang Xu Li Qinglu Zhang Jinghua Xiao Xianghua Li Qifa Zhang Yidan Ouyang national key {2. of Crop Genetic Improvement and national Centre of Plant Gene Research(Wuhan) Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China

Inbreeding depression refers to the reduced performance arising from increased homozygosity,a phenomenon that is the reverse of heterosis and exists among plants and *** a natural self-pollinated crop with strong heterosis,the mechanism of inbreeding depression in rice is largely *** understand the genetic basis of inbreeding depression,we constructed a successive inbreeding population from the F_(2.to F_(4)generation and observed inbreeding depression of all heterotic traits in the progeny along with the decay of heterozygosity in each *** expected depression effect was largely explained by 13 QTLs showing dominant effects for spikelets per panicle,11 for primary branches,and 12.for secondary branches,and these loci constitute the main correlation between heterosis and inbreeding ***,the genetic basis of inbreeding depression is also distinct from that of heterosis,such that a biased transmission ratio of alleles for QTLs with either dominant or additive effects in four segregation distortion regions would result in minor effects in expected ***,two-locus interactions may change the extent and direction of the depression effects of the target loci,and overall interactions would promote inbreeding depression among *** an F_(2.3)variation population,the actual performance of the loci showing expected depression was evaluated considering the heterozygosity decay in the background after *** found inconsistent or various degrees of background depression from the F_(2.to F_(3)generation assuming different genotypes of the target locus,which may affect the actual depression effect of the locus due to *** results suggest that the genetic architecture of inbreeding depression and heterosis is closely linked but also differs in their intrinsic mechanisms,which expand our understanding of the whole-genome architecture of inbreeding depression.

关键词： rice inbreeding depression spikelet development dominance segregation distortion epistasis

Searching for plant NLR immune receptors conferring resistance to potyviruses

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The Crop Journal 2024年第1期12卷 28-44页

作者： Xin Hong Shufen Li Xiaofei Cheng Haijian Zhi Jinlong Yin Kai Xu Jiangsu key laboratory for Microbes and Functional Genomics Jiangsu Engineering and Technology Research Center for MicrobiologyCollege of Life SciencesNanjing Normal UniversityNanjing 210023JiangsuChina College of Plant Protection Northeast Agricultural UniversityHarbin 150030HeilongjiangChina national Center for Soybean Improvement National Key Laboratory for Crop Genetics and Germplasm EnhancementKey Laboratory of Biology and Genetic Improvement of Soybean-Ministry of AgricultureNanjing Agricultural UniversityNanjing 210095JiangsuChina

To fight against invasion by pathogens,plants have evolved an elaborate innate immune system,of which the nucleotide-binding domain leucine-rich repeat-containing receptor(NLR)acts as the sensor and immune ***,comprising one of the largest genera of plant viruses,cause severe crop yield losses *** crop resistance to potyviruses can be used in breeding and plant transgenesis to control disease *** review summarizes achievements in mapping and cloning NLR genes conferring dominant resistance against potyvirus in the families Fabaceae,Solanaceae,Brassicaceae,and *** compares mechanisms of potyviral protein recognition and downstream signaling employed by NLRs and discusses strategies for exploiting NLRs to better control diseases caused by potyviruses.

关键词： NLR Potyvirus resistance Genetic diversity Mapping Engineer

Pig H3K4me3,H3K2.ac,and gene expression profiles reveal reproductive tissue-specific activity of transposable elements

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Zoological Research 2024年第1期45卷 138-151页

作者： Tao Jiang Zhi-Min Zhou Zi-Qi Ling Qing Zhang Zhong-Zi Wu Jia-Wen Yang Si-Yu Yang Bin Yang Lu-Sheng Huang national key {2. of Pig Genetic Improvement and Germplasm Innovation Jiangxi Agricultural UniversityNanchangJiangxi 330045China

Regulatory sequences and transposable elements(TEs)account for a large proportion of the genomic sequences of species;however,their roles in gene transcription,especially tissue-specific expression,remain largely *** serve as an excellent animal model for studying genomic sequence biology due to the extensive diversity among their wild and domesticated ***,we conducted an integrated analysis using H3K2.ac ChIP-seq,H3K4me3 ChIP-seq,and RNA-seq data from 10 different tissues of seven fetuses and eight closely related adult *** aimed to annotate the regulatory elements and TEs to elucidate their associations with histone modifications and mRNA expression across different tissues and developmental *** on correlation analysis between mRNA expression and H3K2.ac and H3K4me3 peak activity,results indicated that H3K2.ac exhibited stronger associations with gene expression than ***,1.45%of TEs overlapped with either the H3K2.ac or H3K4me3 peaks,with the majority displaying tissue-specific ***,a TE subfamily(LTR4C_SS),containing binding motifs for SIX1 and SIX4,showed specific enrichment in the H3K2.ac peaks of the adult and fetal ***-seq analysis also revealed widespread expression of TEs in the exons or promoters of genes,including 4688 TE-containing transcripts with distinct development stage-specific and tissue-specific *** note,1967 TE-containing transcripts were enriched in the *** identified a long terminal repeat(LTR),MLT1F1,acting as a testis-specific alternative promoter in SRPK2.a cell cycle-related protein kinase)in our pig *** element was also conserved in humans and mice,suggesting either an ancient integration of TEs in genes specifically expressed in the testes or parallel evolutionary ***,our findings demonstrate that TEs are deeply embedded in the genome and exhibit important tissue-specific biological functions,particularly in the reproductive

关键词： Transposable elements Porcine Histone modification Alternative promoter TE-containing transcript

Host-induced gene silencing of the Verticillium dahliae thiamine transporter protein gene(VdThit)confers resistance to Verticillium wilt in cotton

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Journal of Integrative Agriculture 2024年第10期23卷 3358-3369页

作者： Qi Wang Guoqiang Pan Xingfen Wang Zhengwen Sun Huiming Guo Xiaofeng Su Hongmei Cheng national key {2. of agricultural microbiology/Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing100081China Xinjiang Jinfengyuan Seeds Co. Ltd.Aksu 843100China College of Agronomy Hebei Agricultural UniversityBaoding 071001China Institute of microbiology Applications Xinjiang Academy of Agricultural SciencesUrumqi 830091China

Verticillium wilt(VW),induced by the soil-borne fungus Verticillium dahliae(Vd),poses a substantial threat to a diverse array of plant *** molecular breeding technology for the development of cotton varieties with heightened resistance to VW stands out as one of the most efficacious protective *** this study,we successfully generated two stable transgenic lines of cotton(Gossypium hirsutum L.),VdThitRNAi-1 and VdThit-RNAi-2.using host-induced gene silencing(HIGS)technology to introduce double-stranded RNA(dsRNA)targeting the thiamine transporter protein gene(VdThit).Southern blot analysis confirmed the presence of a single-copy insertion in each *** examination showed marked reductions in the colonization and spread of Vd-mCherry in the roots of VdThit-RNAi cotton compared to wild type(WT).The corresponding disease index and fungal biomass of VdThit-RNAi-1/2.also exhibited significant ***-time quantitative PCR(qRT-PCR)analysis demonstrated a substantial inhibition of VdThit expression following prolonged inoculation of VdThit-RNAi *** RNA sequencing(sRNA-Seq)analysis revealed the generation of a substantial number of VdThit-specific siRNAs in the VdThit-RNAi transgenic ***,the silencing of VdThit by the siVdThit produced by VdThit-RNAi-1/2.resulted in the elevated expression of multiple genes involved in the thiamine biosynthesis pathway in *** field conditions,VdThit-RNAi transgenic cotton exhibited significantly enhanced disease resistance and yield compared with *** summary,our findings underscore the efficacy of HIGS targeting VdThit in restraining the infection and spread of Vd in cotton,thereby potentially enabling the development of cotton breeding as a promising strategy for managing VW.

关键词： Verticillium dahliae thiamine transporter host-induced gene silencing(HIGS) RNAi

Continued evolution of H10N3 influenza virus with adaptive mutations poses an increased threat to mammals

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Virologica Sinica 2024年第4期39卷 546-555页

作者： Shiping Ding Jiangtao Zhou Junlong Xiong Xiaowen Du Wenzhuo Yang Jinyu Huang Yi Liu Lihong Huang Ming Liao Jiahao Zhang Wenbao Qi State key laboratory for Animal Disease Control and Prevention South China Agricultural UniversityGuangzhou510642China national Avian Influenza Para-Reference laboratory Guangzhou510642China key laboratory of Zoonoses Ministry of Agriculture and Rural AffairsGuangzhou510642China national and Regional Joint Engineering laboratory for Medicament of Zoonoses Prevention and Control Guangzhou510642China key laboratory of Zoonoses Prevention and Control of Guangdong Province Guangzhou510642China State key laboratory of {2. microbiology College of Veterinary MedicineHuazhong Agricultural UniversityWuhan430070China College of Animal Science and Technology Zhongkai University of Agriculture and EngineeringGuangzhou510550China

The H10 subtype avian influenza virus(AIV)poses an ongoing threat to both birds and ***,fatal human cases of H10N3 and H10N8 infections have drawn public *** 2.2.,we isolated two H10N3 viruses(A/chicken/Shandong/0101/2.2. and A/chicken/Shandong/0603/2.2.)from diseased chickens in *** analysis revealed that these viruses were genetically associated with human-origin H10N3 virus,with internal genes originating from local H9N2.*** to the H10N8 virus(A/chicken/Jiangxi/102.2.13),the H10N3 viruses exhibited enhanced thermostability,increased viral release from erythrocytes,and accumulation of hemagglutinin(HA)***,we evaluated the pathogenicity of both H10N3 and H10N8 viruses in *** found that viral titers could be detected in the lungs and nasal turbinates of mice infected with the two H10N3 viruses,whereas H10N8 virus titers were detectable in the lungs and brains of ***,the proportion of double HA Q2.2. and G2.8S mutations in H10N3 viruses has increased since ***,the functional roles of the Q2.2. and G2.8S double mutations in the HA gene of H10N3 viruses remain unknown and warrant further *** study highlights the potential public health risk posed by the H10N3 virus.A spillover event of AIV to humans could be a foretaste of a looming ***,it is imperative to continuously monitor the evolution of the H10N3 influenza virus to ensure targeted prevention and control measures against influenza outbreaks.

关键词： Avian influenza virus(AIV) H10N3 Evolution Pathogenicity

To open early or late:Decoding the mystery of diurnal floret opening time in rice

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Plant Communications 2024年第5期5卷 91-93页

作者： Jian Che Xu Li Yidan Ouyang national key {2. of Crop Genetic Improvement and national Center of Plant Gene Research(Wuhan) Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China

DIFFERENTIATED DIURNAL FLORET OPENING TIME PROMOTES PREZYGOTIC REPRODUCTIVE BARRIERS IN PLANTS As one of the most diverse groups within the plant kingdom,flowering plants have achieved tremendous success on earth and have evolved diversified flowering habits as a result of longterm adaptation to changing *** diurnal floret opening time(DFOT),which represents the time at which a flower opens each day,is a key flowering habit characterized by an internal rhythm and regular environmental cycles.

关键词： diurnal flowering earth

Spatiotemporal evolution and assembly processes of ammonia-oxidizing prokaryotic communities in 1 000-year-old coastal reclaimed soils

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Pedosphere 2024年第4期34卷 709-725页

作者： Sarfraz HUSSAIN Yifan YIN Senlin LIU Shanshan YAN Dongjie CHEN Hui CAO Feng WANG key laboratory of {2. Environmental microbiology Ministry of Agriculture and Rural AffairsCollege of Life SciencesNanjing Agricultural UniversityNanjing 210095China Institute of Eco-Environmental Sciences Ningbo Academy of Agricultural SciencesNingbo 315040China

Coastal marshes are transitional areas between terrestrial and aquatic *** are sensitive to climate change and anthropogenic *** recent decades,the reclamation of coastal marshes has greatly increased,and its effects on microbial communities in coastal marshes have been studied with great *** of these studies have explained the short-term spatiotemporal variation in soil microbial community ***,the impact of reclamation on the community composition and assembly processes of functional microbes(e.g.,ammonia-oxidizing prokaryotes)is often *** this study,using quantitative polymerase chain reaction and the Ion S5™XL sequencing platform,we investigated the spatiotemporal dynamics,assembly processes,and diversity patterns of ammonia-oxidizing prokaryotes in 1000-year-old reclaimed coastal salt *** taxonomic and phylogenetic diversity and composition of ammonia oxidizers showed apparent spatiotemporal variations with soil *** null modelling-based analysis showed that across all sites,the archaeal ammonia-oxidizing community was assembled by a deterministic process(84.71%),and deterministic processes were also dominant(55.2.)for ammonia-oxidizing bacterial communities except for communities at 60 years of *** assembly process and nitrification activity in reclaimed soils were positively *** abundance of the amoA gene and changes in ammonia-oxidizing archaeal and bacterial diversities significantly affected the nitrification activity in reclaimed *** findings suggest that long-term coastal salt marsh reclamation affects nitrification by modulating the activities of ammonia-oxidizing microorganisms and regulating their community structures and assembly *** results provide a better understanding of the effects of long-term land reclamation on soil nitrogen-cycling microbial communities.

关键词： ammonia oxidizer beta nearest taxon index coastal salt marshes community assembly deterministic process nitrification rate stochastic process

Bactericidal bissulfone B7 targets bacterial pyruvate kinase to impair bacterial biology and pathogenicity in plants

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Science China(Life Sciences) 2024年第2期67卷 391-402页

作者： Awei Zhang Haizhen Zhang Ronghua Wang Hongfu He Baoan Song Runjiang Song national key {2. of Green Pesticide Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationCenter for R&D of Fine Chemicals of Guizhou UniversityGuiyang 550025China

The prevention and control of rice bacterial leaf blight(BLB)disease has not yet been achieved due to the lack of effective agrochemicals and available ***,we develop a series of novel bissulfones and a novel target with a unique mechanism to address this *** developed bissulfones can control Xanthomonas oryzae ***(Xoo),and 2.(bis(methylsulfonyl)methylene)-N-(4-chlorophenyl)hydrazine-1-carboxamide(B_(7))is more effective than the commercial drugs thiodiazole copper(TC)and bismerthiazol(BT).Pyruvate kinase(PYK)in Xoo has been identified for the first time as the target protein of our bissulfone B_(7).PYK modulates bacterial virulence via a CRP-like protein(Clp)/two-component system regulatory protein(reg R)*** elucidation of this pathway facilitates the use of B_(7)to reduce PYK expression at the transcriptional level,block PYK activity at the protein level,and impair the interaction within the PYK-Clp-reg R complex via competitive inhibition,thereby attenuating bacterial biology and *** study offers insights into the molecular and mechanistic aspects underlying anti-Xoo strategies that target *** believe that these valuable discoveries will be used for bacterial disease control in the future.

关键词： 2.bissulfone-N-phenylhydrazine-1-carboxamide derivatives Xoo antibacterial activities pyruvate kinase regulatory mechanism

Discovery and structure-activity relationship studies of novel tetrahydro-β-carboline derivatives as apoptosis initiators for treating bacterial infections

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Journal of Integrative Agriculture 2024年第4期23卷 1259-1273页

作者： Shanshan Su Hongwu Liu Junrong Zhang Puying Qi Yue Ding Ling Zhang Linli Yang Liwei Liu Xiang Zhou Song Yang national key {2. of Green Pesticide/key {2. of Green Pesticide and agricultural Bioengineering Ministry of Education/Center for R&D of Fine ChemicalsGuizhou UniversityGuiyang 550025China

Developing and excavating new agrochemicals with highly active and safe is an important tactic for protecting crop health and food *** this paper,to discover the new bactericide candidates,we designed,prepared a new type of1,2.3,4-tetrahydro-β-carboline(THC)derivatives and evaluated the in vitro and in vivo bioactivities against the Xanthomonas oryzae ***(Xoo),Xanthomonas axonopodis ***(Xac),and Pseudomonas syringae ***(Psa).The in vitro bioassay results exhibited that most title molecules possessed good activity toward the three plant pathogenic bacteria,the compound A17 showed the most active against Xoo and Xac with EC50 values of 7.2. and 4.89 mg mL^(-1)respectively,and compound A8 exhibited the best inhibitory activity against Psa with EC50value of 4.87 mg mL^(-1).Pot experiments showed that compound A17 exhibited excellent in vivo antibacterial activities to manage rice bacterial leaf blight and citrus bacterial canker,with protective efficiencies of 52.67 and 79.79%at 2.0 mgmL^(-1),***,compound A8 showed good control efficiency(84.31%)against kiwifruit bacterial canker at 2.0 mg mL^(-1).Antibacterial mechanism suggested that these compounds could interfere with the balance of the redox system,damage the cell membrane,and induce the apoptosis of Xoo *** together,our study revealed that tetrahydro-β-carboline derivatives could be a promising candidate model for novel broadspectrum bactericides.

关键词： tetrahydro-β-carboline derivatives antibacterial activity ROS cell apoptosis