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Enhanced direct gaseous CO_(2) fixation into higher bio-succinic acid production and selectivity

Journal of Environmental Sciences 2024年第9期143卷 164-175页

作者： Ziyi Yang Wanling Wu Qing Zhao Irini Angelidaki Samuel Gyebi Arhin Dongliang Hua Yuxiao Zhao Hangyu Sun Guangqing Liu Wen Wang Biomass Energy and Environmental Engineering research Center Beijing University of Chemical TechnologyBeijing 100029China College of Chemical Engineering Beijing University of Chemical TechnologyBeijing 100029China Department of Chemical and Biochemical Environmental Engineering Technical University of DenmarkDK-2800Kgs LyngbyDenmark Energy research {1. Qilu University of Technology(Shandong Academy of Sciences)Shandong Provincial Key Laboratory of Biomass Gasification TechnologyJinan 250014China

Utilizing CO_(2)for bio-succinic acid production is an attractive approach to achieve carbon capture and recycling(CCR)with simultaneous production of a useful platform *** succinogenes and Basfia succiniciproducens were selected and investigated as microbial ***,the type and concentration of inorganic carbon concentration and glucose concentration were evaluated.6 g C/L MgCO_(3)and 24 g C/L glucose were found to be the optimal basic operational conditions,with succinic acid production and carbon yield of over 30 g/L and over 40%,***,for maximum gaseous CO_(2)fixation,carbonate was replaced with CO_(2)at different ***“less carbonate more CO_(2)”condition of the inorganic carbon source was set as carbonate:CO_(2)=1.9(based on the mass of carbon).This condition presented the highest availability of CO_(2)by well-balanced chemical reaction equilibrium and phase equilibrium,showing the best performance with regarding CO_(2)fixation(about 1. mg C/(L·hr)),with suppressed lactic acid *** to key enzymes analysis,the ratio of phosphoenolpyruvate carboxykinase to lactic dehydrogenase was enhanced at high ratios of gaseous CO_(2),which could promote glucose conversion through the succinic acid *** further increase gaseous CO_(2)fixation and succinic acid production and selectivity,stepwise CO_(2)addition was evaluated.50%-65%increase in inorganic carbon utilization was obtained coupled with 20%-30%increase in succinic acid *** was due to the promotion of the succinic acid branch of the glucose metabolism,while suppressing the pyruvate branch,along with the inhibition on the conversion from glucose to lactic acid.

关键词： Succinic acid CO_(2) fixation Actinobacillus succinogenes Basfia succiniciproducens Lactic acid

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Distributions and accumulation mechanisms of helium in petroliferous basins

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Science China Earth Sciences 2024年第10期67卷 3143-3168页

作者： Pengpeng LI Quanyou LIU Dongya ZHU Di ZHU Zheng ZHOU Xiaoqi WU Qingqiang MENG Jiahao LV Yu GAO institute of Energy Peking UniversityBeijing 100871China Northwest institute of Eco-Environment and Resources Chinese Academy of SciencesLanzhou 730000China Petroleum Exploration&Production research {1. SINOPECBeijing 102206China Energy research {1. Qilu University of Technology(Shandong Academy of Sciences)Jinan 250014China Lancaster Environment Centre Lancaster UniversityLA14YQUK institute of Geology and Geophysics Chinese Academy of SciencesBeijing 100029China University of Chinese Academy of Sciences Beijing 100049China

Helium is an irreplaceable strategic mineral resource, and commercial helium-rich gas fields(He>0.1.) worldwide are typically discovered serendipitously during hydrocarbon exploration efforts. According to an analysis of 75 helium-rich gas fields and 1.48 natural gas samples worldwide, helium in natural gas generally exhibits “scarce”, “accompanying”, and“complex” properties, and helium-rich gas fields often occur at depths 1.). However, geological reserves in the former two types of gas fields are mainly in the range of 1.^(7)–1.^(1.)m^(3), whereas in the latter, they are only in the range of 1.^(5)–1.^(7)m^(3). There are nevertheless notable disparities in the genesis and migration patterns between helium and gaseous hydrocarbons. Helium necessitates carriers(such as formation water, hydrocarbon fluids, N_(2), mantle-derived fluids, etc.) during both accumulation and long-distance migration processes, where migration conduits are not confined to sedimentary strata, and may extend to the basin's basement, lower crust, and even lithospheric mantle. However, the accumulation conditions of both helium and gaseous hydrocarbons are generally considered equivalent. The presence of gaseous hydrocarbons facilitates both the rapid exsolution of helium within helium-containing fluids and subsequent efficient aggregation in gaseous hydrocarbons, while both reduce helium diffusion and diminish escape flux. In terms of caprock, gypsum, salt, and thick shale as sealing layers contribute to the long-term preservation of helium over geological timescales. Large helium-rich gas fields, predominantly crust-derived gas fields, are primarily concentrated in uplifted zones of ancient cratonic basins and their peripheries. Based on a diagram of the He concentration versus He/N_(2) ratio, crust-derived helium fields can be categorized as basement, combined basement-s

关键词： Helium resource Geochemical characteristics Helium source rock Helium supply pattern Accumulation mechanism Determination of favorable zones

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Preparation and Properties of Spray Polyurea Elastomer for Aquaculture Foam Floating Balls

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Agricultural Biotechnology 2024年第2期13卷 38-42页

作者： Guifang HUANG Guanyuan WEI Shengjun WEI Lan DING Wenhong CAI Bolin PAN fisheries {1. institute of Fujian Xiamen 361013China

[Objectives] This study was conducted to develop a polyurea elastomer which can be sprayed on the surface of expanded polystyrene (EPS) floating balls, so as to improve the surface strength and service life of the floating balls. [Methods] The effects of the types and amounts of isocyanate, chain extenders and polyether polyols on the gelation rate, adhesion and wear resistance of polyurea elastomer were investigated, and it was finally determined the preparation process of polyurea elastomer using liquid isophorone diisocyanate (IPDI) and amino-terminated polyether (D2000) as the main raw materials, dimethylthiotoluene diamine (E300) as the chain extender and silica as the wear resistance modifier through two-step solution polymerization of prepolymerization and chain extension. [Results] The physical properties and chemical resistance tests of spray polyurea elastomer showed that it had good physical properties and acid and alkali resistance, and could meet the requirements of spraying and protection of EPS floating ball surface in marine environment. [Conclusions] Polyurea elastomer coating can improve the aging resistance, wear resistance and acid and alkali resistance of EPS floating balls, and prevent them from being fragile and floating randomly to form marine floating garbage which results in "white pollution".

关键词： EPS floating ball Spray polyurea elastomer Gelation rate Wear resistant Adhesion Chemical resistance

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An Ion-Pumping Interphase on Graphdiyne/Graphite Heterojunction for Fast-Charging Lithium-Ion Batteries

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CCS Chemistry 2024年第1期6卷 110-124页

作者： Juan An Fan Wang Jia-Yue Yang Guoxing Li Yuliang Li shandong Provincial Key Laboratory for Science of Material Creation and Energy Conversion Science Center for Material Creation and Energy ConversionSchool of Chemistry and Chemical EngineeringInstitute of Frontier and Interdisciplinary ScienceShandong UniversityQingdao 266237 Optics&Thermal Radiation research Center Institute of Frontier and Interdisciplinary ScienceShandong UniversityQingdao 266237 institute of Chemistry The Chinese Academy of SciencesBeijing 100190 University of Chinese Academy of Sciences Beijing 100049

The sluggish lithium-ion(Li-ion)transport kinetics in graphite anode hinders its application in fast-charging Li-ion batteries(LIBs).Here,we develop an ionpumping interphase(IPI)on graphdiyne(GDY)/graphite heterojunction anodes to boost the ionic transport kinetics and enable high-performance,fast-charging *** IPI changed the ion solvation/desolvation environment by covalent/non-covalent interactions with Li ions or solvents to optimize solid-electrolyte interphase(SEI)and regulate Li-ion transport *** studied the in situ growth of few-layer GDY on graphite surface(GDY/graphite)as the IPI and found that the strong interaction between GDY and Li ions enabled surface-induced modification of the ion solvation behavior and surface-assisted desolvation effect to accelerate the Li-ion desolvation process.A functional anion-derived SEI layer with improved Li-ion conductivity was *** with the generated built-in electric field at GDY/graphite hetero-interface self-pumping Li ions to intercalate into the graphite,the Li-ion transport kinetics was significantly enhanced to effectively eliminate Li plating and large voltage polarization of the graphite anodes.A fast Li intercalation in GDY/graphite without Li oversaturation at the edge of the graphite was directly *** superior performance with high capacity(1.9.2 mA h g^(-1.)and long lifespan(1.50 cycles)under extremely fast-charging conditions(20 C,1.C=372 mA g^(-1.)was achieved on GDY/graphite *** at low temperatures(-20℃),a specific capacity of 1.8.4 mA h g^(-1. was achieved with a capacity retention of 80%after 500 cycles at a 2 C rate.

关键词： extreme fast-charging lithium-ion batteries lithium-ion transport kinetics ion-pumping interphase graphdiyne/graphite heterojunction

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A novel Effective Panicle Number per Plant 4 haplotype enhances grain yield by coordinating panicle number and grain number in rice

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The Crop Journal 2024年第1期12卷 202-212页

作者： Yun Wang Xiaoqian Wang Laiyuan Zhai Sundus Zafar Congcong Shen Shuangbing Zhu Kai Chen Yun Wang Jianlong Xu State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory of Crop Biology College of AgronomyShandong Agricultural UniversityTai’an 271018ShandongChina Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120GuangdongChina Rice research {1. Shenyang Agricultural UniversityShenyang 110866LiaoningChina National Nanfan research {1.(Sanya) Chinese Academy of Agricultural SciencesSanya 572024HainanChina

Increasing effective panicle number per plant(EPN)is one approach to increase yield potential in ***,molecular mechanisms underlying EPN remain *** this study,we integrated mapbased cloning and genome-wide association analysis to identify the EPN4 gene,which is allelic to NARROW LEAF1.NAL1..Overexpression lines containing the Teqing allele(TQ)of EPN4 had significantly increased ***-EPN4^(TQ) in japonica(geng)cultivar Lemont(LT)exhibited significantly improved EPN but decreased grain number and flag leaf size relative to *** analysis indicated that accessions with EPN4-1.had medium EPN,medium grain number,and medium grain weight,but had the highest grain yield among seven haplotypes,indicating that EPN4-1.is an elite haplotype of EPN4 for positive coordination of the three components of grain ***,accessions carrying the combination of EPN4-1.and haplotype GNP1.6 of GNP1.for grain number per panicle showed higher grain yield than those with other allele ***,pyramiding of EPN4-1.and GNP1.6 could be a preferred approach to obtain high yield potential in breeding.

关键词： Rice Effective panicle number per plant Grain number per panicle Haplotype Grain yield potential

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Morphological and molecular analyses of a Philine kinglipini outbreak in summer of 2022 in Jiaozhou Bay,China

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Journal of Oceanology and Limnology 2024年第3期42卷 831-848页

作者： Zelin DUAN Yanan YU Jing WANG Junlong ZHANG Nansheng CHEN CAS Key Laboratory of marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory of marine Ecology and Environmental Science Laoshan LaboratoryQingdao 266237China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China Laboratory of marine Organism Taxonomy and Phylogeny Qingdao Key Laboratory of Marine Biodiversity and ConservationInstitute of OceanologyChinese Academy of SciencesQingdao 266071China marine Biological Museum Chinese Academy of SciencesQingdao 266071China Department of Molecular Biology and Biochemistry Simon Fraser UniversityBurnabyBritish ColumbiaV5A 1S6Canada

A sudden and unprecedented outbreak of molluscan Philine kinglipini occurred in summer 2022 in Jiaozhou Bay,shandong,China,causing substantial damage to local mariculture industry of the Manila clam Ruditapes *** *** has been found in many coastal regions of China,the molecular {1. of *** has not been extensively studied,making it difficult to accurately identify and track *** samples in field using molecular *** were collected during the outbreak and their morphological features and molecular sequences were *** show that the causative species of the outbreak was *** mitochondrial genome(mt DNA)of *** was constructed for the first time,based on which phylogenetic analysis of the mt DNAs of *** and related species in the order Cephalaspidea was carried *** revealed by metabarcoding analysis of 1.S rDNA V4,the seasonal change of *** and closely related Philine species was striking with peaks between April and ***,metabarcoding analysis is applicable tool for monitoring the bloom development of *** and related *** study generated for the first time essential molecular marker sequences and mtDNA of ***,which provided a reference for future characterization and monitoring of its outbreaks and for phylogenetic analysis of Philine species.

关键词： marine species outbreaks metabarcoding analysis mitochondrial genome Philine kinglipini phylogenetics spatial-temporal dynamics

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JrATHB-1. mediates JrMYB1.3 and JrMYB27 to control the anthocyanin levels in different types of red walnut

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Journal of Integrative Agriculture 2024年第8期23卷 2649-2661页

作者： Haifeng Xu Guifang Wang Xinying Ji Kun Xiang Tao Wang Meiyong Zhang Guangning Shen Rui Zhang Junpei Zhang Xin Chen Key Laboratory of Huanghuai Protected Horticulture Engineering/National Germplasm Repository of Walnut and Chestnut Shandong Institute of PomologyTai’an 271000China research {1. of Forestry Chinese Academy of ForestryBeijing 100091China College of Horticulture and Forestry Tarim UniversityAlaer 843300China

Red walnut has broad market prospects because it is richer in anthocyanins than ordinary ***,the mechanism driving anthocyanin biosynthesis in red walnut is still *** studied two types of red walnut,called red walnut 1.R1.,with a red pericarp and seed coat,and red walnut 2(R2),with a red seed coat only.R1.mostly contained cyanidin-3-O-galactoside,while R2 contained a various amounts of cyanidin-3-Ogalactoside,cyanidin-3-O-arabinoside,and *** LDOX-2(LOC1.90071.3)and LDOX-3(LOC1.901.746)genes,which encode leucoanthocyanidin dioxygenase/anthocyanidin synthase(LDOX/ANS),were preliminarily indicated as the crucial genes for anthocyanin biosynthesis in R1.and R2,*** MYB differential genes analysis showed that MYB27 and MYB1.3 are specifically expressed in the red parts of R1.and R2,respectively,and they are regarded as candidate regulatory *** expression in Arabidopsis and transient injection in walnut showed that both MYB27 and MYB1.3 were located in the nucleus and promoted anthocyanin accumulation,while MYB27 promoted the expression of LDOX-2,and MYB1.3 promoted the expression of LDOX-3and *** one-hybrid and electrophoretic mobility shift assays showed that MYB27 could only bind to the LDOX-2 promoter,while MYB1.3 could bind to the promoters of both LDOX-3 and *** addition,we also identified an HD-Zip transcription factor,ATHB-1.,which is specifically expressed in the *** silencing the expression of ATHB-1.,the R2 pericarp turned red,and MYB1.3 expression *** experiments showed that ATHB-1. could specifically interact with MYB1.3 and bind to its *** suggests that MYB27controls R1.coloration by regulating LDOX-2,while MYB1.3 controls R2 coloration by regulating LDOX-3 and UAGT-3,but ATHB-1. can specifically bind to and inhibit the MYB1.3 of the R2 pericarp so that it becomes *** study reveals the anthocyanin biosynthetic mechanisms in two differ

关键词： JrATHB-1. JrMYB1.3 JrMYB27 red walnut anthocyanin biosynthesis

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Changes in the Non-targeted Metabolomic Profile of Three-year-old Toddlers with Elevated Exposure to Polycyclic Aromatic Hydrocarbons

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Biomedical and Environmental Sciences 2024年第5期37卷 479-493页

作者： LI Yang LIN Dan ZHANG Xiu Qin JU Guang Xiu SU Ya ZHANG Qian DUAN Hai Ping YU Wei Sen WANG Bing Ling PANG Shu Tao Department of Epidemiology and Health Statistics School of Public HealthQingdao UniversityQingdao 266021ShandongChina Department of Environmental Health Qingdao Municipal Center for Disease Control and PreventionQingdao Institute of Preventive MedicineQingdao 266033ShandongChina Physicochemical Laboratory Qingdao Municipal Center for Disease Control and PreventionQingdao Institute of Preventive MedicineQingdao 266033ShandongChina Section of Child Health Care Huangdao Maternity and Child Health Care Hospital of QingdaoQingdao 266499ShandongChina Qingdao Blood Center Qingdao 266071ShandongChina

Objective To investigate changes in the urinary metabolite profiles of children exposed to polycyclic aromatic hydrocarbons(PAHs)during critical brain development and explore their potential link with the intestinal *** Liquid chromatography-tandem mass spectrometry was used to determine ten hydroxyl metabolites of PAHs(OH-PAHs)in 36-month-old ***,37 children were categorized into low-and high-exposure groups based on the sum of the ten ***-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to identify non-targeted metabolites in the urine ***,fecal flora abundance was assessed by 1.S rRNA gene sequencing using Illumina *** The concentrations of 21.metabolites were significantly higher in the high exposure group than in the low exposure group(variable importance for projection>1.P1..The identified differential metabolites primarily belonged to pathways associated with inflammation or proinflammatory states,including amino acid,lipid,and nucleotide ***,these distinct metabolites were significantly associated with specific intestinal flora abundances(r=0.34–0.55),which were mainly involved in *** Higher PAH exposure in young children affected metabolic homeostasis,particularly that of certain gut microbiota-derived *** investigation is needed to explore the potential influence of PAHs on the gut microbiota and their possible association with neurodevelopmental outcomes.

关键词： Child Gut microbiota Non-targeted metabolomics Polycyclic aromatic hydrocarbons Urinary metabolite profile

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The RING zinc finger protein LbRZF1.promotes salt gland development and salt tolerance in Limonium bicolor

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Journal of Integrative Plant Biology 2024年第4期66卷 787-809页

作者： Zongran Yang Ziwei Zhang Ziqi Qiao Xueying Guo Yixuan Wen Yingxue Zhou Chunliang Yao Hai Fan Baoshan Wang Guoliang Han shandong Provincial Key Laboratory of Plant Stress College of Life SciencesShandong Normal UniversityJi'nan 250014China National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land Agricultural High-tech Industrial Demonstration Area of the Yellow River Delta of Shandong ProvinceDongying 257000China Dongying institute Shandong Normal UniversityDongying 257000China

The recretohalophyte Limonium bicolor thrives in high-salinity environments because salt glands on the above-ground parts of the plant help to expel excess ***,we characterize a nucleus-localized C3HC4(RING-HC)-type zinc finger protein of *** named RING ZINC FINGER PROTEIN 1.LbRZF1..LbRZF1.was expressed in salt glands and in response to NaCl ***1.showed no E3 ubiquitin ligase *** phenotypes of overexpression and knockout lines for LbRZF1.indicated that LbRZF1.positively regulated salt gland development and salt tolerance in ***1.mutants had fewer salt glands and secreted less salt than did the wild-type,whereas LbRZF1.overexpressing lines had opposite phenotypes,in keeping with the overall salt tolerance of these plants.A yeast two-hybrid screen revealed that LbRZF1.interacted with LbCATALASE2(LbCAT2)and the transcription factor LbMYB1.3,leading to their *** of LbCAT2 or LbMYB1.3 decreased salt gland density and salt *** heterologous expression of LbRZF1.in Arabidopsis thaliana conferred salt tolerance to this *** also identified the transcription factor LbMYB48 as an upstream regulator of LbRZF1.*** study of LbRZF1.in the regulation network of salt gland development also provides a good foundation for transforming crops and improving their salt resistance.

关键词： LbRZF1 Limonium bicolor molecular mechanism salt gland development salt tolerance

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foxl2l is a germ cell-intrinsic gatekeeper of oogenesis in zebrafish

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Zoological research 2024年第5期45卷 1116-1130页

作者： Zhiqin Ren Ding Ye Naike Su Chaofan Wang Lijia He Houpeng Wang Mudan He Yonghua Sun Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture Hubei Hongshan LaboratoryInstitute of HydrobiologyInnovation Academy for Seed DesignChinese Academy of SciencesWuhanHubei 430072China School of marine Biology and {1. Hainan UniversityHaikouHainan 570228China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China College of fisheries and Life Science Dalian Ocean UniversityDalianLiaoning 116023China

Zebrafish serve as a valuable model organism for studying germ cell biology and reproductive *** AB strain of zebrafish is proposed to exhibit a polygenic sex determination system,where most males initially develop juvenile ovaries before committing to male *** species with chromosomal sex determination,gonadal somatic cells are recognized as key determinants of germ cell ***,the loss of germ cells in zebrafish leads to masculinization,implying that germ cells harbor an intrinsic feminization ***,the specific signal triggering oogenesis in zebrafish remains *** the present study,we identified foxl2l as an oocyte progenitor-specific gene essential for initiating oogenesis in germ *** showed that foxl2l-knockout zebrafish bypassed the juvenile ovary stage and exclusively developed into fertile *** analysis revealed that loss of foxl2l hindered the initiation of oocyte-specific meiosis and prevented entry into oogenesis,leading to premature spermatogenesis during early gonadal ***,while mutation of the pro-male gene dmrt1.led to fertile female differentiation,simultaneous disruption of foxl2l in dmrt1.mutants completely blocked oogenesis,with a large proportion of germ cells arrested as germline stem cells,highlighting the crucial role of foxl2l in ***,this study highlights the unique function of foxl2l as a germ cell-intrinsic gatekeeper of oogenesis in zebrafish.

关键词： foxl2l dmrt1 Oogenesis Sex determination Meiosis

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