检索结果-南通市图书馆

International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 147-153页

作者： Junjie Li Wenbo Yu Zhenyu Sun Weichen Zheng Liangwei Zhang Yanling Xue Wenning Liu Shoumei Xiong Center of Materials Science and Engineering School of Mechanical and Electronic Control EngineeringBeijing Jiaotong UniversityBeijing 100044China Traffic&Transportation Engineering Central South UniversityChangsha 410075China Shanghai Synchrotron radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204China School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

High pressure die casting(HPDC)AlSi1.Mn Mg alloy castings are widely used in the automobile *** can optimize the mechanical properties of castings through heat treatment,while the release of thermal stress arouses the deformation of large integrated ***,the development of non-heat treatment Al alloys is becoming the hot *** addition,HPDC contains externally solidified crystals(ESCs),which are detrimental to the mechanical properties of *** achieve high strength and toughness of non-heat treatment die-casting Al-Si alloy,we used AlSi9Mn alloy as matrix with the introduction of Zr,Ti,Nb,and *** influences on ESCs and mechanical properties were systematically investigated through three-dimensional reconstruction and thermodynamic *** results reveal that the addition of Ti increased ESCs'size and porosity,while the introduction of Nb refined ESCs and decreased ***,large-sized Al_3(Zr,Ti)phases formed and degraded the mechanical *** introduction of Ce resulted in the poisoning effect and reduced mechanical properties.

关键词： aluminium alloy high-pressure die-casting externally solidified crystals non-heat treatment

Gene therapy for rat renal anemia with implantation of erythropoietin-transgenic myoblasts

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 1999年第1期42卷 109-112页

作者：刘永学魏汉东吴祖泽贺福初 1. beijing institute of radiation medicine 100850 Beijing China

To investigate whether an erythropoietin (EPO) gene-based therapy could serve as an alternative to the repeated injection of rhEPO in treatment to renal anemia, the genetically modified myoblasts of rats, named Myo/ EPO, were implanted through intramuscular injection to model rats with renal anemia. The hemoglobin (Hb) and hematocrit (HCT) of the rats increased from (92. 5±3.0) g/L and 0.29 ±0.04 to the peak values of (1.3.8 ±5.0) g/L and 0. 32 ±0. 04 respectively 1. d after implantation, and sustained the pre-implantation level for 90 d. Otherwise, the control rats implanted with Myo/X, which carried the parent retroviral vector, gradually became severe in anemia. The PCR detection for hEPO cDNA in the rat muscle adjacent to injection sites indicated that the Myo/EPO cells survived for a long period in the muscle of rats. The results primarily demonstrate that myoblast gene transfer of EPO is effective for the treatment of rat renal anemia.

关键词： rat renal anemia erythropoietin gene therapy.

Mechanism of p53 downstream effectors p21.and Gadd45 in DNA damage surveillance

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 1999年第4期42卷 427-434页

作者：孟祥兵董燕孙志贤 1. Department of Biochemistry Institute of Radiation Medicine 100850 Beijing China

Both p21. WAF1.CIP1. and Gadd45 were activated in a p53-dependent manner in MCF-7 cells after being exposed to ionizing radiation. In order to investigate their roles in DNA damage surveillance, p21.s/MCF-7 cells stably transfected by p21.antisense expression plasmid pC-WAFl-AS and Gadd45as/MCF-7 stably transfected by Gadd45 antisense expression plasmid pCMVas45 were established. It was observed that G1.arrest induced by radiation was significantly reduced in Gadd45as/MCF-7 cells as well as in p21.s/MCF-7 cells. Repair of radiation damaged report gene greatly reduced in Gadd45asMCF-7 and p21.s/MCF-7 cells. Apoptosis significantly increased in p21.s/MCF-7 after exposure to radiation. These results suggest that both p21.and Gadd45 support cellular survival by taking roles in G1.arrest and DNA repair, furthermore, p21.protects cells from death by inhibiting apoptosis after exposure to ionizing radiation.

关键词： p21 Gadd45 G1 arrest DNA repair apoptosis.

High-dose dexamethasone regulates microglial polarization via the GR/JAK1.STAT3 signaling pathway after traumatic brain injury

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第9期20卷 2611-2623页

作者： Mengshi Yang Miao Bai Yuan Zhuang Shenghua Lu Qianqian Ge Hao Li Yu Deng Hongbin Wu Xiaojian Xu Fei Niu Xinlong Dong Bin Zhang Baiyun Liu beijing Tiantan Hospital Capital Medical UniversityBeijingChina Department of Neurology The First Hospital of Tsinghua UniversityBeijingChina Department of Neurotrauma and beijing Key Laboratory of Central Nervous System Injury Beijing Neurosurgical InstituteCapital Medical UniversityBeijingChina Department of Critical Care medicine Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Center for Nerve Injury and Repair Beijing Institute of Brain DisordersChina National Clinical Research Center for Neurological DiseasesBeijingChina

Although microglial polarization and neuroinflammation are crucial cellular responses after traumatic brain injury,the fundamental regulatory and functional mechanisms remain insufficiently *** potent anti-inflammato ry agents,the use of glucoco rticoids in traumatic brain injury is still controversial,and their regulatory effects on microglial polarization are not yet *** the present study,we sought to determine whether exacerbation of traumatic brain injury caused by high-dose dexamethasone is related to its regulatory effects on microglial polarization and its mechanisms of *** vitro cultured BV2 cells and primary microglia and a controlled cortical impact mouse model were used to investigate the effects of dexamethasone on microglial ***,dexamethasone,RU486(a glucocorticoid receptor antagonist),and ruxolitinib(a Janus kinase 1.antagonist)were ***-sequencing data obtained from a C57BL/6 mouse model of traumatic brain injury were used to identify potential targets of *** Morris water maze,quantitative reverse transcription-polymerase chain reaction,western blotting,immunofluorescence and confocal microscopy analysis,and TUNEL,Nissl,and Golgi staining were performed to investigate our ***-throughput sequencing results showed that arginase 1.a marker of M2 microglia,was significantly downregulated in the dexamethasone group compared with the traumatic brain injury group at3 days post-traumatic brain *** dexamethasone inhibited M1.and M2 microglia,with a more pronounced inhibitory effect on M2microglia in vitro and in *** receptor plays an indispensable role in microglial polarization after dexamethasone treatment following traumatic brain ***,glucocorticoid receptor activation increased the number of apoptotic cells and neuronal death,and also decreased the density of dendritic spines.A possible downstream receptor signaling mechanism is th

关键词： apoptosis BV2 microglia dexamethasone glucocorticoid receptor glucocorticoids innate immune system microglial polarization neuroinflammation primary microglia traumatic brain injury

Therapeutic angiogenesis induced by human hepatocyte growth factor gene in hindlimb ischemia of dogs

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2003年第7期48卷 676-680页

作者： HA Xiaoqin, REN Jianping, BI Jianjin, ZHANG Jinhui & WU Zuze (Wu Chu-tse) beijing {1. of radiation medicine, beijing 1.0850, China 1. beijing institute of radiation medicine Beijing 100850 China

A preclinical study of treating peripheral ar-tery occlusive disease (PAD) was performed by using ahepatocyte growth factor (HGF) gene-expressing vector,plasmid pUDKH, in a dog model with complete ischemia of one hindlimb. After ligation of femoral artery of onehindlimb, pUDKH was transferred directly into the ischemic limb muscles. The angiogenic activity of the plasmid pUDKH was evaluated. On D 30 after injecting once of pUDKH at different doses into local muscles immediately after opera-tion, the degree of augmentation of collateral vessel forma-tion was significantly greater than that treated by blankvector. In addition, the blood flow rate of femoral artery in dogs treated with pUDKH was recovered on D 90, while the flow rate was only 1.5 to 1.3 in control dogs. The pulse am-plitude of pUDKH-treated dogs was recovered on D 90, but it was hardly detectable in most of the control dogs. The sideeffects of intramuscular transfection of pUDKH were alsoinvestigated, and no significant positive change was found. It is suggested that angiogenesis induced by HGF gene has the potential for clinical use in the treatment of peripheral arte-rial diseases.

关键词：外周动脉闭塞疾病肝细胞生长因子基因治疗动物模型下肢局部缺血血管生成

Differentiation potential of bone marrow mesenchymal stem cells into retina in normal and laser-injured rat eye

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2004年第3期47卷 241-250页

作者： ZHANG Jie1.2, SHAN Qing1. MA Ping1. JIANG Yanming1. CHEN Peng1. WEN Jingxia1. ZHOU You1. QIAN Huanwen1.& PEI Xuetao2 1. beijing institute of radiation medicine, {1. 1.0850, China 2. {1. institute of Transfusion medicine, {1. 1.0850, China 1. beijing institute of radiation medicine 100850 Beijing China 2. beijing {1. of Transfusion medicine 100850 Beijing China

Bone marrow mesenchymal stem cells (MSCs) can develop into hematopoietic and mesenchymal lineages but have not been known to participate in the production of retina. Here we report that bone marrow mesenchymal stem cells, after being subretinally transplanted into normal or Nd: YAG laser-injured rat eye, can integrate into RPE layer, photoreceptor layer, bi-polar cell layer and ganglion layer. DAPI-labeling detection was used to trace the origin of the repopulating cells. DAPI fluorescence was used to identify retina cells of bone marrow origin 1., 20, 35 and 50 days after transplantation. No formation of rosettes was found but some random cells were found at the end of the observation. MSCs-originated cells spread more widely in the injured retinas than in the normal ones. Immunohistochemical detection showed that though the cells could express neuronal nuclei (NeuN), neuron specific enolase (NSE), glial fibrillary acidic protein (GFAP) and cytokeratin (CK), the proteins expression in the injured transplantation group was abnormal in some region compared with that in the normal transplantation group. Elec-troretinogram (ERG) showed that ERG-b wave of the injured transplantation group is significantly higher than that of the two laser-injured control groups. These results suggest that a proportion of MSCs can differentiate into retina-like structure in vivo and the differentiation differs in normal and laser-injured retinas.

关键词： MSCs, retina, laser, differentiation.

Unraveling brain aging through the lens of oral microbiota

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第7期20卷 1930-1943页

作者： Qinchao Hu Si Wang Weiqi Zhang Jing Qu Guang-Hui Liu State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Hospital of Stomatology Guanghua School of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders Xuanwu HospitalCapital Medical UniversityBeijingChina Aging Translational medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijingChina CAS Key Laboratory of Genomic and Precision medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijingChina University of Chinese Academy of Sciences BeijingChina institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijingChina Aging Biomarker Consortium BeijingChina State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijingChina beijing {1. for Stem Cell and Regenerative medicine BeijingChina

The oral cavity is a complex physiological community encompassing a wide range of *** of oral microbiota can lead to various oral infectious diseases,such as periodontitis and tooth decay,and even affect systemic health,including brain aging and neurodegenerative *** studies have highlighted how oral microbes might be involved in brain aging and neurodegeneration,indicating potential avenues for intervention *** this review,we summarize clinical evidence demonstrating a link between oral microbes/oral infectious diseases and brain aging/neurodegenerative diseases,and dissect potential mechanisms by which oral microbes contribute to brain aging and *** also highlight advances in therapeutic development grounded in the realm of oral microbes,with the goal of advancing brain health and promoting healthy aging.

关键词： Alzheimer's disease brain aging multiple sclerosis neurodegeneration neurodegenerative diseases oral microbiota Parkinson's disease periodontitis bacteria Porphyromonas gingivalis

Construction of a fusion protein between N-terminal 1.3 peptide of thrombopoietin and erythropoietin

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 1998年第4期41卷 426-434页

作者：卢柏松柳晓兰黄培堂 1. institute of Biotechnology 100071 Beijing China 2. institute of {1. medicine 100850 Beijing China

Thrombopoietin (TPO) functions as a regulator of megakaryocytes in their differentiation and maturation, and is a candidate pharmaceutical for the curing of thrombocytopenia. Erythropoietin (EPO) is a hematopoietic cytokine that regulates the level of red blood cell. It is widely used in renal anemia and tumor associated anemia, and is proved to be safe and effective. In order to study the possibility of using TPO EPO fusion protein for the curing of anemia and thrombocytopenia induced by high dose chemotherapy, a fusion gene is constructed by linking TPO N terminal 1.3 peptide and EPO mature peptide coding region. The fusion gene is expressed in mammalian cells, revealing that the expression product can support the growth of TPO responsive Ba/F3 mpl cells and EPO dependent Bet 2 cells in the absence of any other stimulating cytokine. It also stimulates the formation of erythroid colonies and megakaryocytic colonies in semi solid bone marrow cultures. These results indicate that the fusion protein has both the in vitro activities of TPO and EPO. The preliminary in vivo experiment reveals that the TPO EPO fusion protein containing cell supernatant raises the platelet level by 37% in mice, while its function on erythroid hematopoiesis remains to be determined. These results indicate that the construction of TPO EPO fusion protein and the further study of its use in high dose chemotherapy are possible

关键词： thrombopoietin erythropoietin fusion protein.

Mechanism of inflammatory response and therapeutic effects of stem cells in ischemic stroke:current evidence and future perspectives

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第1期20卷 67-81页

作者： Yubo Wang Tingli Yuan Tianjie Lyu Ling Zhang Meng Wang Zhiying He Yongjun Wang Zixiao Li Vascular Neurology Department of NeurologyBeijing Tiantan HospitalCapital Medical UniversityBeijingChina China National Clinical Research Center for Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Shanghai Engineering Research Center of Stem Cells Translational medicine ShanghaiChina National Center for Healthcare Quality Management in Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina institute for Regenerative {1. Shanghai East HospitalSchool of Life Sciences and TechnologyTongji UniversityShanghaiChina Chinese institute for Brain Research BeijingChina Research Unit of Artificial Intelligence in Cerebrovascular Disease Chinese Academy of Medical SciencesBeijingChina beijing Engineering Research Center of Digital Healthcare for Neurological Diseases BeijingChina

Ischemic stroke is a leading cause of death and disability worldwide,with an increasing trend and tendency for onset at a younger ***,in particular,bears a high burden of stroke *** recent years,the inflammatory response after stroke has become a research hotspot:understanding the role of inflammatory response in tissue damage and repair following ischemic stroke is an important direction for its *** review summarizes several major cells involved in the inflammatory response following ischemic stroke,including microglia,neutrophils,monocytes,lymphocytes,and ***,we have also highlighted the recent progress in various treatments for ischemic stroke,particularly in the field of stem cell ***,understanding the complex interactions between inflammation and ischemic stroke can provide valuable insights for developing treatment strategies and improving patient *** cell therapy may potentially become an important component of ischemic stroke treatment.

关键词： cell therapy immune cell inflammatory ischemic stroke stem cell

Construction of serial deletants and chimeras of multi-kringle containing molecules and primary analysis of their functions

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 1999年第5期42卷 548-553页

作者：宫锋董春娜张咏章杨培吴祖泽贺福初 1. beijing institute of radiation medicine 100850 Beijing China 2. beijing {1. of Blood Transfusion medicine 100850 Beijing China

In comparison of amino acid sequences of 4 kringles of both macrophage stimulating protein (MSP) and hepatocyte growth factor (HGF), consensus motif sequence was determined. According to this consensus sequence, a pair of universal primers were designed. In combination with specific upstream or downstream primer of MSP or HGF respectively, serial fragments containing variant number of kringle (from 1.to 4) can be obtained by once PCR. By ligating the C terminal and N terminal fragments with different combination, serial deletants and chimeras of MSP and HGF were constructed. Sequence analysis showed that the degeneracy for universal primers and the sequences of those constructed deletants and chimeras are desired. Biological assay of these deletants revealed that wild type MSP can inhibit the growth of some tumor cell lines and that kringle 1.of MSP is essential for function as that of HGF.

关键词： kringle HGF MSP deletant chimera.