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Apoptosis of human leukemic HL-60 cells induced to differentiate by treatment with RA or DMSO

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Cell Research 1995年第2期5卷 181-186页

作者： SUN HONG YONGCHAO WANG(Key Laborutory of Cell Proliferation and Regulation Biology, Beijing Normal University, Beijing 100875, China.) Key Laboratory of Cell Proliferation and Regulation Biology Beijing Normal University Beijing 100875 China.&#x0002A This work was supported by the National Medical and Scientific Key Item Grant of “ 8.5 Plan” .received 9november1994 Revised 13June1995 Accepted 17September1995

In present study we studied the effect of all-trans retinoic acid(ATRA) and dimethylsulfoxide (DMSO) on the induction of apoptosis in HL-60 cell line. Based on morphological changes by Hochest 33342 staining and identification of internucleosomal DNA cleavage by gel electrophoresis, we observed aberrant nuclear chromatin eondensation and ladder-like pattern of DNA degradation. Using Flow Cytometric method, we found sub-G1 peak in RA-treated HL-60 cells starting 5 to 6 d after the initiation of the treatment. However, such an obvious apoptotic peak was not identified in DMSO-differentiated cells. Combining the research accomplished before, our study approves further that apoptosis could be a common mode of death of terminally differentiated HL-60 cells.

关键词： Apoptosis HL-60 cell RA DMSO

Changes of macromolecular organizations in nonjunctional sarcolemmas after cross-innervation──a study offast-and slow-twitch muscle fibres in rats

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Cell Research 1995年第2期5卷 143-154页

作者： LU JIANLI TIEFENG ZHANG FUJIN QU BEIHUA QIAN SHIKAI HUANG(Shanghai Institute of Physiology, Chinesc Academy of Scicnces, 320 Yue-Yang Road, Shanghai 200031, China) Shanghai Institute of Physiology Chinese Academy of Sciences 320 Yue-Yang Road Shanghai 200031 China.Received 11January1994 Revised 12December1994 Accepted 16February1995

The purpose of the present study was to analyse the changes of macromolecular organizations in nonjunctional sarcolemmas of different types of skeletal muscle fibres after cross-innervation. In normal rats the mean density of square arrays (6 nm particles organized in orthogonal arrays) was 9.02/μm2 for the nonjunctional sarcolemmas of fast-twitch extensor digitorum longus (control EDL,CE) nuscle fibres and 0.34/μm2 for the nonjunctional sarcolemmas of slow-twitch soleus (control SOL, CS) muscle fibres. After cross-innervation between the fast-twitch EDL and slow-twitch SOL muscle fibres by slow and fast muscle merves respectively for three months, the mean density was 0.45/μm2 for the nonjunetional sarcolemmas of the operated-EDL (OE) and 8.3/μm2 for the nonjunctional sarcolemmas of the operated SOL (OS). This indicates that the cross-innervation causes a reciprocal transformation of the number and distribution of such macromolecular organizations in the electrically excitable nonjunctional sarcolemmas.

关键词： Sarcolemma square array cross-innervation freeze-fracturing rat(Wistar)

Plant regeneration from protoplasts of hydroxyprolineresistant cell line in Onobrychis viciaefolia

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Cell Research 1995年第2期5卷 187-194页

作者： XU ZIQIN JINGFEN JIA(Cell Blology Laboratory, Lanzhou University, Lanzhou 730000, China.) Cell Biology Laboratory Lanzhou University Lanzhou 730000 China.Received 30August1994 Revised 27April1995 Accepted 25July1995

An efficient protocol for plant regeneration from protoplasts of hydroxyproline(HYP)resistant cell line of Onoblychis viciaefolia was established. In SH medium supplemented with 1 mg/L 2, 4-dichlorophenoxy-acetic acid (2,4-D), 0.5 mg/L kinetin (KT) and 0.2 mg/L naphthalene acetic acid (NAA), the division frequency of protoplastderived cells reached uP to over 60 %, and microcalli were obtained in 5-6 wk. Upon transferring them on agar solidified MS medium plus 2 mg/L indole-3-acetic acid (IAA), shoots were induced. After cultivating them on MS medium with or without IAA, roots were *** number of all protoplast-regenerated plants examined were normal (2n=28). The protoplast-derived calli and plants grew vigorously on the medium containing 10 mmol/L HYP.

关键词： Sainfoin (Onobrychis Viciaefolia) hydroxyproline resistant cell line protoplast culture plant regeneration

Mesozoic magmatism and copper polymetallic mineralization processes in the Shanghang-Datian region, Fujian Province, Southeast China

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Chinese Journal Of Geochemistry 2006年第3期25卷 266-278页

作者：毛建仁胡青许乃政陈荣叶海敏赵希林 Nanjing Institute of Geology and Mineral Resources Nanjing 210016 China GeoligicalPartyofWestFujianProvince(1994)Min-eralogeneticseriesandprospectingtargetareaofZijinshanCu-AudepositinFujianProvince.

Various isotopic ages were reported for four granite plutons in the area studied, i.e., the Tangquan pluton (185-158 Ma); the Zijinshan pluton (157-145 Ma); the Caixi pluton (133 Ma); and the Sifang pluton (108-105 Ma). The Tangquan and Zijinshan plutons belong to Early-Middle Jurassic and Late Jurassic of Early-Mesozoic respectively, and the Caixi and Sifang plutons belong to Early-Cretaceous of Late Mesozoic. Both the Tangquan and Sifang plutons, which are the metaluminous granites with A/CNK{1.1}, and possesses the geochemical features of crust-derived muddy materials resultant from partial melting with enrichments in K, Rb, Th, Y, HREE and depletions in Sr, Ba, and Ti. The Caixi pluton is a weakly peraluminous granite with A/CNK={1.0}-{1.1}, and its geochemical characteristics are intermediate between the Zijinshan pluton and the Sifang pluton. The Tangquan pluton with higher Na-2O contents, which resulted from partial melting of Proterozoic thick lower-crust derived basic rocks in response to underplating of mantle-derived basic magma, is one of the major host rocks of the metallogenic series of copper, iron and polymetallic deposits. The Sifang pluton with higher K-2O contents, which was mixed between middle-lower crust-derived granite magma by partial melting and mantle-derived high-K basic magma, giving rise to the increment of K-2O in the melt, and extracted large amounts of copper and other metals, as well as sulphur, is one of the major host rocks of the metallogenic series of porphyry copper, gold and polymetallic deposits. Petrological records have shown that there has occurred lithosphere extension since the Early-Middle Jurassic (180 Ma) in the region, and the formation of Zijinshan peraluminous granite

关键词：花岗岩中生代镁铜金属混合物福建矿化过程

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Substance P enhances the proliferation of rat anteriorpituitary cells in vitro

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Cell Research 1995年第2期5卷 197-207页

作者： ZHANG WANHUI YUNLONG ZHU FUZHOU WANG YUZHEN HU QI MEI CHAO ZHAO JIANGKANG CHEN(Laboratory of Neuroendocrinology, Department of Physiology, The 4th Military Medical University, Xi'an 710032,China.) Laboratory of Neuroendocrinology Department of Physiology The 4th Military Medical University Xi'an 710032 China.Received 24October1994 Revised 8June1995 Accepted 20October1995

The undecapeptide substance P(SP) was shown to be intimately involved in both the structural and functional aspects of the anterior pituitary. Yet, in addition to its influences on hormonal secretion, SP may well possess more actions in this master gland. The present study was ftherefore aimed to investigate the effect of SP on the proliferation of rat anterior pituitary cells in primary culture. It was found that SP could dose-dependently increase the ineorporation of tritiated thymidine (3H-TdR)into cultured anterior pituitary cells. Other mammalian tachykinins such as neurokinin A and neurokinin B had similar effect but to varying degrees. The equipotent analogue of SP, Norleucine(11) -SP(Nle(11)-SP), also acted likewise, with its action antagonizable by spantide, a SP receptor blocker. To further characterize the nature of cells responsive to the challenge of SP, immunocytochemical staining against S-100 protein and some adenohypophyseal hormones was performed alone or plus autoradiography The results showed that the percentage of S-100 proteinimmunoreactive cells was apparently elevated by the addtion of Nle(11)-SP for 48 h, which indicates a preferential proliferation of folliculo-stellate cells under the regime. This was confirmed by increases in immunocytochemical or autoradiographical labelling indices of anterior pituitary cells treated similarly. Taken togethbr, these results reveal that the trophic action of SP observed previously in other tissues is also present at least in cultured rat anterior pituitary cells, with responding cells being predominantly folliculo-stellate cells as typified by S-100 proteinimmunoreactivity. Therefore, an intra-pituitary trophic action of SP in vivo could be anticipated.

关键词： Substance P anterior pituitary proliferation in vitro rat

Characterization of Giardia cell nucleus:Its implication on the nature and origin of the primitive cell nucleus

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Cell Research 1995年第1期5卷 115-124页

作者： LI JINGYAN (Department of Evolutionary Cell Biology, Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.) Department of Evolutionary Cell Biology Laboratory of Cellular and Molecular Evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223 China.Received 20October1994 Revised 8June1995 Accepted 18June1995

Looking For the Equilibrium through the Game with Life

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中国金融家 2005年第7期 60-61页

作者： John F. Nash The Laureate of 1994 Nobel Memorial Prize in Economic Science Jr.

Affinity chromatography-dependent selection (ACDS)of genomic DNA fragments bound specifically to bacterial synthesized Myc/Myn proteins

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Cell Research 1995年第1期5卷 25-34页

作者： SHI CAN PEI WANG YONGJUN HU LIAN XU. (Oncogene Group, Laboratory of Molecular and Cellular Oncology, Shanghai Institute of Cell Biology, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China) Oncogene Group Laboratory of Molecular and Cellular Oncology Shanghai Institute of Cell BiologyChinese Academy of Sciences 320 Yue-Yang RoadShanghai 200031 China&#x00023 ]Both made equal contribution to the work.* The work is specifically dedicated to Prof. Zhen YAO for his 80-years birthday.† Supported by grant No. 39270742 of the National Natural Science Foundation.received 20December1994 Revised 21April1995 Accepted 26April1995

This paper describes an approach to seek for mouse c-Myc/Myn proteins-bound specific sequences among ge-nomic DNA. cDNA fragment of myn gene was obtained through RT-PCR technique from RNA of NIH3T3 cells. DNA fragments encoding BR/HLH/LZ structure of Myc and Myn proteins were cloned in frame into pGEX-2T vec-tor respectively Fusion GST-Myc and GST-Myn synthe-sized in *** hosts showed affinity to CACGTG E-boxDNA and subsequently interacted with genomic fragments prepared through whole-genome-PCR. A PCR-assisted procedure which combines protein-DNA interaction and affinity chromatography was designed to enrich Myc/Myn bound DNA. At least two genomic DNA fragments ob- tained exhibit specifical binding capacity to Myc/Myn complex but not to GST alone. Significance of the work and of the technique itself as well as identification of the DNAs are discussed.

关键词： Myc/Myn proteins ACDS genomic DNA binding

CLIMATIC CHARACTERISTICS OF ATMOSPHERIC NET RADIATION OVER CHINA

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Acta meteorologica Sinica 1997年第2期11卷 246-254页

作者：翁笃鸣高庆先姚志国 Department of Applied Meteorology Nanjing Institute of Meteorology.Nanjing 210044 Graduated in 1994 from Nanjing Institute of Meteorology majoring in climatology

In terms of ERBE and ISCCP *** measured/calculated surface net radiation, computation is performed of the climatic characteristics of ANR(atmospheric net radiation)across China alongside with the discussion and relations to other two kinds of radiation and contributing *** suggests a high linear correlation of ANR with atmosphere-absorbed shortwave *** can be established a general expression for ANR,which decreases more sharply as a function of altitude and increases slightly with latitude in summer,and changes uniformly in ***,a comparison is made of the findings presented in this paper and literatures regarding the ANR pattern and magnitudes,indicating their great difference.

关键词： China atmospheric net radiation(ANR) climatic characteristics

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