Apoptosis of human leukemic HL-60 cells induced to differentiate by treatment with RA or DMSO

作     者：SUN HONG YONGCHAO WANG(Key Laborutory of Cell Proliferation and Regulation Biology, Beijing Normal University, Beijing 100875, China.) 

作者机构：Key Laboratory of Cell Proliferation and Regulation Biology Beijing Normal University Beijing China 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：1995年第5卷第2期

页      面：181-186页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

主　　题：Apoptosis HL-60 cell RA DMSO 

摘      要：In present study we studied the effect of all-trans retinoic acid(ATRA) and dimethylsulfoxide (DMSO) on the induction of apoptosis in HL-60 cell line. Based on morphological changes by Hochest 33342 staining and identification of internucleosomal DNA cleavage by gel electrophoresis, we observed aberrant nuclear chromatin eondensation and ladder-like pattern of DNA degradation. Using Flow Cytometric method, we found sub-G1 peak in RA-treated HL-60 cells starting 5 to 6 d after the initiation of the treatment. However, such an obvious apoptotic peak was not identified in DMSO-differentiated cells. Combining the research accomplished before, our study approves further that apoptosis could be a common mode of death of terminally differentiated HL-60 cells.