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Efficient production of D-1,2,4-butanetriol from D-xylose by engineered Escherichia coli whole-cell biocataiysts

Frontiers of Chemical Science and Engineering 2018年第4期12卷 772-779页

作者： Shewei Hu Qian Gao Xin Wang Jianming Yang Nana Xu Kequan Chen Sheng Xu Pingkai Ouyang State Key Laboratory of Materials-Oriented Chemical Engineering College of Bioteclmology and Pharmaceutical Engineering Nanjing Teeh UniversityNanjing 211816China Xi'an Modem Chemistry Research Institute Xi'an 710065China

We have developed a whole-cell bioconversion system for the production of d-1,2,4-butanetriol (BT) from renewable biomass. A plasmid pETduet-xylB-yjhG-T7-adhP-T7-mdlC was constructed and transformed to Escherichia coli BL21(DE3) to obtain the whole cells of E. coli BL21-XYMA capable of bioconversion d-xylose to BT. Then, the factors including carbon sources, nitrogen sources, metal ions, and culture conditions (pH, temperature, IPTG) were identified, and their effects on the whole-cell activity for BT production were investigated. To obtain the highest whole-cell activity, the optimal cultivation parameters are: 15 gL(-1) yeast extract, 5 gL(-1) sucrose, 3 gL(-1) KH2PO4, 5 gL(-1) NaCl, 3 gL(-1) NH4Cl, 0.25 gL(-1) MgSO(4)7H(2)O and 1 mLL(-1) the mixture of trace elements. With the optimized whole cells of E. coli BL21-XYMA, 60 gL(-1) of xylose was converted to 28 gL(-1) BT with a molar yield of 66 %, which is higher than those reported in the biotechnological system.

关键词： d-1 2 4-butanetriol whole-cell bioconversion carbon source nitrogen sources metal ions culture conditions bioconversion Nitrogen sources metal ions biocatalysts CARBON SOURCES cell activity Escherichia coli Deep Space 1 xylopyranose whole cell yeast extracts

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Electrophysiology of hyperpolarization-activated cyclic nucleotidegated cation channel 2 and hyperpolarization-activated cyclic nucleotide-gated cation channel 4 expressed in HEK293 cells

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Chinese Medical Journal 2007年第22期120卷 2039-2041页

作者： LI Chun GUO Ji-hong LI Ji-wen LIU Yuan-wei HAO Xue-mei ZHANG Ping WANG Shi-qiang Division of Cardiac Electrophysiology Department of Internal Medicine Beijing University People＇s Hospital Beijing 100044 China State Key Laboratory of Biomembrane and Membrane Biotechnology College of Life Sciences Peking University Beijing 100084 China

The action potential of pacemaker cells in the sino-atrial node forms the automatic rhythm of the heart. The automatic depolarization in phase 4 is me DaSlS of the automaticity in pacemaker cells. Many currents are included in phase 4, such as calcium current, TTX-sensitive sodium current, sustained inward current （Isi）, decay of delayed rectifier potassium current, etc. Funny current （If） has long been recognized as important in this phase and is activated at hyperpolarized potentials during cell diastole and in turn activates other currents to form automatic depolarization.

关键词： hyperpolarization-activated cyclic nucleotide-gated potassium channel HEK293 cell whole cell

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