Efficient production of D-1,2,4-butanetriol from D-xylose by engineered Escherichia coli whole-cell biocataiysts
作者机构：State Key Laboratory of Materials-Oriented Chemical EngineeringCollege of Bioteclmology and Pharmaceutical Engineering Nanjing Teeh UniversityNanjing 211816China Xi'an Modem Chemistry Research InstituteXi'an 710065China
出 版 物：《Frontiers of Chemical Science and Engineering》 (化学科学与工程前沿（英文版）)
年 卷 期：2018年第12卷第4期
页 面：772-779页
核心收录：
学科分类：0817[工学-化学工程与技术] 08[工学] 0703[理学-化学]
基 金：the Open Funding Project of the State Key Laboratory of Bioreactor Engineering,the National Natural Science Foundation of China (Grant Nos.21576134, 21606127,21390200) the National Key Research and Development Program of China (Grant No.2016YFA0204300)
主 题：d-1 2 4-butanetriol whole-cell bioconversion carbon source nitrogen sources metal ions culture conditions bioconversion Nitrogen sources metal ions biocatalysts CARBON SOURCES cell activity Escherichia coli Deep Space 1 xylopyranose whole cell yeast extracts
摘 要：We have developed a whole-cell bioconversion system for the production of d-1,2,4-butanetriol (BT) from renewable biomass. A plasmid pETduet-xylB-yjhG-T7-adhP-T7-mdlC was constructed and transformed to Escherichia coli BL21(DE3) to obtain the whole cells of E. coli BL21-XYMA capable of bioconversion d-xylose to BT. Then, the factors including carbon sources, nitrogen sources, metal ions, and culture conditions (pH, temperature, IPTG) were identified, and their effects on the whole-cell activity for BT production were investigated. To obtain the highest whole-cell activity, the optimal cultivation parameters are: 15 gL(-1) yeast extract, 5 gL(-1) sucrose, 3 gL(-1) KH2PO4, 5 gL(-1) NaCl, 3 gL(-1) NH4Cl, 0.25 gL(-1) MgSO(4)7H(2)O and 1 mLL(-1) the mixture of trace elements. With the optimized whole cells of E. coli BL21-XYMA, 60 gL(-1) of xylose was converted to 28 gL(-1) BT with a molar yield of 66 %, which is higher than those reported in the biotechnological system.
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