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Enhancement of fruit byproducts through bioconversion by Hermetia llucens(Diptera:Stratiomyidae)

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Insect Science 2023年第4期30卷 991-1010页

作者： Carmen Scieuzo Antonio Franco Rosanna Salvia Micaela Triunfo Nicola Francesco Addeo Simone Vozzo Giovanni Piccolo Fulvia Bovera Alberto Ritieni Antonio Di Francia Ambrogio Laginestra Eric Schmitt Patrizia Falabella Department of Sciences University of BasilicataPotenzaItaly Spinoff XFlies s.r.l University of BasilicataPotenzaItaly Department of Veterinary Medicine and Animal Production University of Naples Federico IlNaplesItaly Department of Pharmacy University of Naples Federico llNaplesItaly Departmentof Agriculture University of Naples Federico llPorticiItaly Department of Relations with the Territory TotalEnergies EP Italia S.p.APotenzaItaly Protix B.V. DongenThe Netherlands

bioconversion is a biological process by which organic materials are converted into products with higher biological and commercial value.During its larval stage the black soldier fly Hermetia illucens is extremely voracious and can feed on a wide vari-ety of organic materials.To study the impact of different fruit byproducts on the insect's growth,final larval biomass,substrate reduction,bioconversion parameters,and larval nu-tritional composition,10000 black soldier fly larvae(BSFL)were reared on 7.0 kg of one of three substrates(strawberry,tangerine,or orange)or on a standard diet as a control.The results highlight that BSFL can successfully feed and grow on each of these diets,though their development time,growth rate,and final biomass were differently impacted by the substrates,with strawberry being the most suitable.The lipid and protein contents of BSFL were similar among larvae fed on different substrates;however,major differences were detected in ash,micronutrient,fiber,fatty acid,and amino acid contents.Overall,the results indicate that fruit waste management through the BSFL bioconversion process rep-resents a commercially promising resource for regional and national agrifood companies.Our study offers new perspectives for sustainable and environmentally friendly industrial development by which fruit byproducts or waste might be disposed of or unconventionally enhanced to create secondary products of high biological and economic value,including BSFL biomass as animal feed or,in perspective,as alternative protein source for human nutrition.

关键词： bioconversion black soldier fly byproducts circular economy feed

Self-assembly of metal-cholesterol oxidase hybrid nanostructures and application in bioconversion of steroids derivatives

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Frontiers of Chemical Science and Engineering 2021年第3期15卷 615-629页

作者： Yu Xin Qiuyue Gao Yu Gu Mengyao Hao Guangming Fan Liang Zhang Key Laboratory of Industry Biotechnology(Ministry of Education) School of BiotechnologyJiangnan UniversityWuxi 214122China National Engineering Laboratory for Cereal Fermentation Technology Jiangnan UniversityWuxi 214122China

A cholesterol oxidase(COD)was hybridized with Ca^(2+),Zn^(2+),Al^(3+),Fe^(2+) and Mn^(2+).After precipitation with PO_(4)^(3-) at 4℃ for 72 h,the resulting pellets were freeze-dried.In scanning electron microscopy assays,the metal-COD complexes revealed flower-like or granular structures after hybridization.Fourier transform infrared spectroscopy assay revealed the characteristic peaks of both the enzyme and metal materials.X-ray diffraction analysis indicated that COD was encapsulated in CaHPO_(4)·2H_(2)O-,Zn_(3)(PO_(4))_(2)·4H_(2)O-,AlPO_(4-),FeP_(4-) and Mn_(3)(PO_(4))_(2)·3H_(2)O-based nanostructures,respectively.Differential scanning calorimetry assay indicated significant increases in thermo-denaturation temperatures from 60.5℃ to 167.02℃,167.02℃,137.70℃,172.85℃ and 160.99℃,respectively.Using steroid derivatives as substrates,this enzyme could convert cholesterol,pregnenolone,dehydroepiandrosterone,ergosterol,b-sitosterol and stigmasterol to related single products.Hybridization in metal-based nanostructures could significantly enhance the initial conversion ratio and reaction stability of the enzyme.In addition,substrate selectivity could be affected by various metal materials.Briefly,using Ca^(2+),Zn^(2+),Al^(3+),Fe^(2+) and Mn^(2+) as hybrid raw materials could help to encapsulate COD in related metal-enzyme nanostructures,and could help to promote the stability and tolerant properties of the enzyme,while also enhancing its catalytic characteristics.

关键词： cholesterol oxidase metal-enzyme hybridization nanostructures sterol derivatives bioconversion

The bioconversion of Municipal Solid Waste in the Biodrying Reactor

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Journal of Environmental & Earth Sciences 2019年第1期1卷 33-37页

作者： Maria Zgadlo Marlena Debicka Jolanta Latosińska Kielce University of Technology Faculty of EnvironmentalGeomatic and Energy EngineeringDepartment of Waste Managemental.Tysiaclecia PP 725-314 KielcePoland

The bioconversion process of municipal solid waste was assessed on the basis of the results obtained from the biodrying reactor working at a full industrial scale.The bio-reactor is a part of mechanical-biological installation following mechanical stage.The bio-reactor was equipped with measuring devices allowing the analysis of the parameters like:temperature both inside the waste and also air above the waste and also the humidity of waste during the 14 days of the biodrying process.The kinetics of bioconversion was assessed basing on measured the loss of ignition(LOI)parameter detected during the biodrying process.The LOI value of the samples varied from 17.03%Am.to 30.34%d.***.depending on the location inside the reactor.The estimated kinetic rate constant kT of the bioconversion of biomass in the industrial reactor was kT=0.3141.In analyzed case study the calorific value of product leaving the full-scale bio-reactor is too low to use this product as an alternative fuel.As w'as stated the reason of this is too low a share of the carbon-rich fraction in the feedstock.

关键词： Biodrying of waste Mechanical-biological treatment(MBT) Kinetics of waste bioconversion

Phenolic Acids from <i>Parthenium hysterophorus</i>: Evaluation of bioconversion Potential as Free Radical Scavengers and Anticancer Agents

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Advances in Bioscience and Biotechnology 2015年第1期6卷 11-17页

作者： Richa Panwar Asvene Kumar Sharma Dharm Dutt Vikas Pruthi Department of Biotechnology Indian Institute of Technology Roorkee Roorkee India Department of Paper Technology Indian Institute of Technology Roorkee Roorkee India

Parthenium hysterophorus is a globally recognized invasive alien weed that prominently colonizes grazing areas and cultivated lands causing adverse effect on crop production. Major allelochemicals released from parthenium include sesqueterpene lactones and phenolic acids. Among these the presence of caffeic, vanillic and ferulic acids is of industrial significance as they possess potent free radical scavenging and anticancer activities. This study reports for the first time, high total phenolic acid content (20.82 ± 0.82 mg GAE/g dry sample) in parthenium. The GC-MS analysis indicated the presence of ferulic, p-coumaric, vanillic and gallic acid as major phenolic components. Free radical scavenging activity of the phenolic acids extract gave an EC50 value 130.4 μg/ml when measured using DPPH assay. Anticancer activity of parthenium phenolic extract against A-498 (IC50 0.5237 μg/ml) and MDA-MB231 (IC50 and 0.2685 μg/ml) cancerous cell lines indicated its potential to be used as anticancer agent.

关键词： Parthenium hysterophorus Phenolic Acids GC-MS Antioxidant bioconversion

Effects of Different Genotypes of Switchgrass as a Bioenergy Crop on Yield Components and bioconversion Potential

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Journal of Sustainable Bioenergy Systems 2017年第1期7卷 27-35页

作者： Doohong Min Yadhu N. Guragain Vara Prasad Praveen V. Vadlani Jungeun Lee Department of Agronomy Kansas State University Manhattan KS USA Department of Grain Science & Industry Kansas State University Manhattan KS USA

Switchgrass (Panicum virgatum L.) is a native warm-season grass and it is one of potential bioenergy crops. The objectives of this study were to: 1) assess the best performing switchgrass genotype suitable for Kansas soil and climatic condition in the USA, 2) determine the correlation between plant height or tiller numbers per plant and dry biomass of various switchgrass genotypes, and 3) assess a bioconversion efficiency of certain varieties of switchgrass. Twenty-two different genotypes of seedlings were allowed to grow in cones for 30 days under controlled environments. The genotype Cave-in-Rock was the shortest among the genotypes. Significant difference in number of tillers per plant was observed among the genotypes. The genotypes Alamo recorded the highest numbers of tiller plant-1 and the genotype Cave-in-Rock had the lowest numbers of tiller plant-1 compared with other genotypes. The genotypes Alamo, NL 94 C2-2, NL 94 C2-3, NSL 2009-1 and NSL 2009-2 had increased above ground biomass compared with other genotypes. The correlation study indicates that there was a significant positive correlation between number of tillers per plant and per plant dry weight (R2 = 0.93), number of tillers per plant and plant height (R2 = 0.94), and plant height and per plant dry weight (R2 = 0.82). Based on the biomass composition, the SWG 2007-2 genotype was the promising switchgrass line for the bioconversion through the sugar platform route due to high carbohydrate and low lignin content. On the other hand, the high biomass yield per unit area of field in NL 94 C2-1 led this genotype with the highest total carbohydrate yield per unit area of field despite the lowest total carbohydrate content in the genotype. These results are pertinent for crop breeders to develop the most promising switchgrass line with high biomass yield and high bioconversion efficiency to produce biofuel through the sugar platform route.

关键词： Switchgrass Genotype Bioenergy Crop Plant Height Tiller Numbers Yield bioconversion

Production of bio-energy from low-value biomass by bioconversion

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Engineering Sciences 2014年第3期12卷 14-16页

作者： Tan Tianwei Beijing University of Chemical Technology Beijing 100029 China

Since 1993, China has become a net importer of energy from a net exporter. The total energy con- sumption has been greater than the total supply, and the external dependence of the energy demand increases rapidly. China＇ s crude oil import volume and imports amount reached 253.78 million tons and 196.664 billion US dollars in 2011, with a growth rate of 6 % and 45.3 %, respectively, year-on-year. The significant increase in demand for oil and the caused structural contradictions are increasingly becoming the greatest challenge for China＇ s energy security. The energy crisis has not only touched everyone＇ s nerves, but also sparked a strong desire to find alternative energy.

关键词： bio-energy low-value biomass bioconversion

Palladium and carbon synergistically catalyzed room-temperature hydrodeoxygenation(HDO) of vanillyl alcohol–A typical lignin model molecule

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Journal of Energy Chemistry 2017年第1期26卷 8-16页

作者： Qi Wang Neeraj Gupta Guodong Wen Sharifah Bee Abd Hamid Dang Sheng Su School of Materials Science and Engineering University of Science and Technology of China Institute of Metal Research Chinese Academy of Sciences Nanotechnology & Catalysis Research Centre (NANOCAT) University of Malaya IPS Building

Vanillyl alcohol, which is made up of an aromatic ring, an alcoholic hydroxyl group, a phenolic hydroxyl group and a methoxy group, was selected as the model molecule of lignin. Various carbon materials supported Pd catalysts were chosen to catalyze the HDO of vanillyl alcohol. The catalysts were characterized via TEM, TPD, XRD, XPS and CO-chemisorption. It was found that different carbon materials could obviously influence the particle sizes, dispersion and distribution of Pd or PdO particles. Palladium and carbon can synergistically catalyze the room-temperature HDO of vanillyl alcohol even at room temperature, and the carboxyl group was found to be the effective active acid site during the reaction. Possible reaction mechanism was also proposed. The existence of the effective active acid sites on the carbon supports could obviously lower the reaction temperature without decreasing the selectivity, as a result, making the production of renewable fuels by HDO much more economically feasible, which is of much importance. ? 2016 Science Press

关键词： bioconversion Carbon Catalysts Dispersions Molecules Palladium

Ring-Opening Polymerization of ε-Caprolactone Catalyzed by Diisopropylamido Bis(methylcyclopentadienyl) Erbium Tetrahydrofuranate Complex

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Journal of Rare Earths 2000年第2期18卷 158-160页

作者：薛明强沈琪张振江毛礼胜 Department of Chemistry and Chemical Engineering Suzhou University Suzhou 215006 China

Ring-opening polymerization of Ε-caprolactone was successfully carried out by diisopropylamido bis (methylcyclopentadienyl) erbium as the catalyst. It is found that organoerbium amide is a very active catalyst for Ε-caprolactone ring-opening polymerization. The activity increases with the increasing of polymerization temperature. Under certain conditions, the polymerization system shows a living characteristic.

关键词： bioconversion Catalysis Erbium Medical applications Rare earths

Efficient production of cyclic adenosine monophosphate from adenosine triphosphate by the N-terminal half of adenylate cyclase from Escherichia coli

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Chinese Journal of Chemical Engineering 2020年第8期28卷 2167-2172页

作者： Chen Ma Jing Wang Xuelin Wang Dandan Mai Yuqi Jin Kequan Chen Xin Wang Pingkai Ouyang State Key Laboratory of Materials-Oriented Chemical Engineering College of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjing 211816China

In this study,we aimed at developing an efficient biocatalytic process for bio-production of cyclic adenosine monophosphate(c AMP)from adenosine triphosphate(ATP).First,adenylate cyclase from Escherichia coli MG1655(EAC)and Bordetella Pertussis(BAC)were expressed in E.coli BL21(DE3)and comparatively analyzed for their activities.As a result,EAC from E.coli MG1655 exhibited a higher activity.However,amount of EAC were obtained in an insoluble form.Therefore,we expressed the first 446 amino acids of EAC(EAC446)to avoid the inclusion body.The effects of induction temperature,incubation time,and incubation p H were further evaluated to improve the expression of EAC446.Subsequently,the reaction process for the production of c AMP with ATP as a starting material was investigated.As none of c AMP was detected in the whole-cell based biocatalytic process,the reaction catalyzed by the crude enzyme was determined for c AMP production.What's more,the reaction temperature,reaction p H,metal ion additives and substrate concentration was optimized,and the maximum c AMP production of 18.45 g·L^-1was achieved with a yield of 95.4%after bioconversion of 6 h.

关键词： Adenylate cyclase Cyclic adenosine monophosphate(cAMP) Adenosine triphosphate(ATP) bioconversion