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Design and Evaluation of A Novel HSV-2 epitope Vaccine

Design and Evaluation of A Novel HSV-2 epitope Vaccine

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中国生物工程学会第十二届学术年会暨2018年全国生物技术大会

作者： Wenwen Zeng Yong Qi Jiameng Li Suyun Liu Shulong Zheng Jixian Rao Wanpeng Shen Yuexi Li Department of Medical and Pharmaceutical Biotechnology Huadong Research Institute for Medicine and Biotechniques School of Life Science and Technology China Pharmaceutical University

We used LCP technology to design and synthesize a lipid core peptide vaccine with self-assembly and intramolecular adjuvant properties for the pre-expressed HSV-2 epitope, and evaluate the immune effect. In this study, LCPs were designed for two B cell epitopes gB466-473(EQDRKPRN) and gC216-223(GRTDRPSA), respectively named P1-(K)2-LC and P2-(K)2-LC, *** other peptide including P1-P25, P1-P25-KLH, P2-P25, P2-P25-KLH. Mice in nine groups were intramuscularly immunized with 30 μg of each antigen per mousein the hind limbs with P1-(K)2-LC, P1-P25, P1-P25-KLH, and MF59 adjuvanted P1-P25, P2-(K)2-LC, P2-LC, P2-P25, P2-P25-KLH, and MF59 adjuvanted P2-P25 respectively, In peptide 1 and peptide 2 experiment, mice were immunized for three times. Anti-P1-BSA or anti-P2-BSA IgG, IgG1, and IgG2 a antibody titers were evaluated using ELISA and analyzed statistically in sera after each immunization. P1-(K)2-LC and P1-P25-KLH induced the highest IgG and IgG1 antibody titer. IgG and IgG1 titers induced by P2-(K)2-LC were higher than other groups after the second or third immunization. However, none peptide groups induced IgG2 a *** the T cell responses, CD4 and CD8 T cells isolated from immunized mice were stimulated with corresponding peptides and cytokines in the supernatant were detected. As a result, none of the cytokines were *** HSV-2 virus neutralization activity of mouse serum after the last immunization was measured by a 50% plaque reduction assay. P1-(K)2-LC induced antisera performed a higher neutralization titer than the other three *** summary, this study designed, synthesized, and evaluated two lipid epitopes of B-cell epitopes that all showed good humoral immunity. P1-(K)2-LC and P2-LC could stimulate mice into producing specific antibodies that could neutralize HSV-2 infection in *** results suggest that the lipid core peptide vaccine may be used for vaccine design against HSV-2.

关键词： HSV-2 (Herpes simplex virus type 2) humoral immunity cellular immunity virus-neutralizing activity

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Design and evaluation of a novel HSV-2 epitope vaccine

Design and evaluation of a novel HSV-2 epitope vaccine

引用

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Wenwen Zeng Yong Qi Jiameng Li Suyun Liu Jixain Rao Wanpeng Shen Shulong Zheng Yuexi Li Huadong Research Institute for Medicine and Biotechniques School of Life Science and Technology China Pharmaceutical University

We used LCP technology to design and synthesize a lipid core peptide vaccine with self-assembly and intramolecular adjuvant properties for HSV-2 epitope,and evaluate the immune *** this study,LCPs were designed for two B cell epitopes gB466-473(EQDRKPRN) and gC216-223(GRTDRPSA),respectively named LCP-1 and LCP-2 a,LCP-2 *** other peptide including P1-P25,P1-P25-KLH,P2-P25,*** in nine groups were intramuscularly immunized with 30 μg of each antigen per mouse in the hind limbs with LCP1,P1-P25,P1-P25-KLH,and MF59 adjuvanted P1-P25,LCP2 a,LCP2 b,P2-P25,P2-P25-KLH,and MF59 adjuvanted P2-P25 respectively,In peptide 1 experiment,mice were immunized for four times on days 0,21 st,35 th,and 49 th *** peptide 2 experiment,mice were immunized for three ***-P1-BSA or anti-P2-BSA IgG,IgG1,and IgG2 a antibody titers were evaluated using ELISA and analyzed statistically in sera after each ***1 and P1-P25-KLH induced the highest IgG and IgG1 antibody *** and IgG1 titers induced by LCP2 a were higher than other groups after the second or third ***,none peptide groups induced IgG2 a *** the T cell responses,CD4 and CD8 T cells isolated from immunized mice were stimulated with corresponding peptides and cytokines in the supernatant were *** a result,none of the cytokines were *** HSV-2 virus neutralization activity of mouse serum after the last immunization was measured by a 50% plaque reduction ***1-induced antisera performed a higher neutralization titer than the other three *** summary,this study designed,synthesized,and evaluated two lipid epitopes of B-cell epitopes that all showed good humoral ***1 and LCP2 b could stimulate mice into producing specific antibodies that could neutralize HSV-2 infection in *** results suggest that the lipid core peptide vaccine may be used for vaccine design against HSV-2.

关键词： Herpes simplex virus type 2(HSV-2) humoral immunity cellular immunity virus-neutralizing activity

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