Design and Evaluation of A Novel HSV-2 epitope Vaccine
作者单位：Department of Medical and Pharmaceutical Biotechnology Huadong Research Institute for Medicine and Biotechniques School of Life Science and Technology China Pharmaceutical University
会议名称：《中国生物工程学会第十二届学术年会暨2018年全国生物技术大会》
会议日期：2018年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学]
基 金：support from China Key Project of New Medicine Development (2014ZX09304314-003 2015ZX09J15105-001-003) China Natural Science Foundation (81373109) China Jiangsu Province Social Development Foundation (No. BE2016622)
关 键 词：HSV-2 (Herpes simplex virus type 2) humoral immunity cellular immunity virus-neutralizing activity
摘 要：We used LCP technology to design and synthesize a lipid core peptide vaccine with self-assembly and intramolecular adjuvant properties for the pre-expressed HSV-2 epitope, and evaluate the immune effect. In this study, LCPs were designed for two B cell epitopes gB466-473(EQDRKPRN) and gC216-223(GRTDRPSA), respectively named P1-(K)2-LC and P2-(K)2-LC, *** other peptide including P1-P25, P1-P25-KLH, P2-P25, P2-P25-KLH. Mice in nine groups were intramuscularly immunized with 30 μg of each antigen per mousein the hind limbs with P1-(K)2-LC, P1-P25, P1-P25-KLH, and MF59 adjuvanted P1-P25, P2-(K)2-LC, P2-LC, P2-P25, P2-P25-KLH, and MF59 adjuvanted P2-P25 respectively, In peptide 1 and peptide 2 experiment, mice were immunized for three times. Anti-P1-BSA or anti-P2-BSA IgG, IgG1, and IgG2 a antibody titers were evaluated using ELISA and analyzed statistically in sera after each immunization. P1-(K)2-LC and P1-P25-KLH induced the highest IgG and IgG1 antibody titer. IgG and IgG1 titers induced by P2-(K)2-LC were higher than other groups after the second or third immunization. However, none peptide groups induced IgG2 a *** the T cell responses, CD4 and CD8 T cells isolated from immunized mice were stimulated with corresponding peptides and cytokines in the supernatant were detected. As a result, none of the cytokines were *** HSV-2 virus neutralization activity of mouse serum after the last immunization was measured by a 50% plaque reduction assay. P1-(K)2-LC induced antisera performed a higher neutralization titer than the other three *** summary, this study designed, synthesized, and evaluated two lipid epitopes of B-cell epitopes that all showed good humoral immunity. P1-(K)2-LC and P2-LC could stimulate mice into producing specific antibodies that could neutralize HSV-2 infection in *** results suggest that the lipid core peptide vaccine may be used for vaccine design against HSV-2.