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Use of a tissue clearing technique combined with retrograde trans-synaptic viral tracing to evaluate changes in mouse retinorecipient brain regions following optic nerve crush

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Neural Regeneration Research 2023年第4期18卷 913-921页

作者： Zong-Yi Zhan Yi-Ru Huang Lu-Wei Zhao Ya-Dan Quan Zi-Jing Li Di-Fang Sun Ya-Li Wu Hao-Yuan Wu Zi-Tian Liu Kai-Li Wu Yu-Qing Lan Min-Bin Yu Department of Ophthalmology Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene RegulationSun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Light Innovation Technology Ltd. Hong Kong Special Administrative RegionChina Department of Ophthalmology&Visual Science Shanghai Medical CollegeFudan UniversityShanghaiChina Department of Ophthalmology Eye&ENT HospitalFudan UniversityShanghaiChina Department of Ophthalmology The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina

Successful establishment of reconnection between retinal ganglion cells and retinorecipient regions in the brain is critical to optic nerve ***,morphological assessments of retinorecipient regions are limited by the opacity of brain *** this study,we used an innovative tissue cleaning technique combined with retrograde trans-synaptic viral tracing to observe changes in retinorecipient regions connected to retinal ganglion cells in mice after optic nerve ***,we performed light-sheet imaging of whole brain tissue after a clearing *** found that pseudorabies virus 724(PRV724)mostly infected retinal ganglion cells,and that we could use it to retrogradely trace the retinorecipient regions in whole tissue-cleared ***,PRV724-traced neurons were more widely distributed compared with data from previous *** found that optic nerve injury could selectively modify projections from retinal ganglion cells in the hypothalamic paraventricular nucleus,intergeniculate leaflet,ventral lateral geniculate nucleus,central amygdala,basolateral amygdala,Edinger-Westphal nucleus,and oculomotor nucleus,but not the superior vestibular nucleus,red nucleus,locus coeruleus,gigantocellular reticular nucleus,or facial nerve *** findings demonstrate that the tissue clearing technique,combined with retrograde trans-synaptic viral tracing,can be used to objectively and comprehensively evaluate changes in mouse retinorecipient regions that receive projections from retinal ganglion cells after optic nerve ***,our approach may be useful for future estimations of optic nerve injury and regeneration.

关键词： histology image analysis light-sheet imaging optic nerve crush pseudorabies virus retinal ganglion cells three-dimensional imaging tissue clearing viral tracing whole brain study

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Lighting Up Neural Circuits by viral tracing

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Neuroscience Bulletin 2022年第11期38卷 1383-1396页

作者： Liyao Qiu Bin Zhang Zhihua Gao Department of Neurobiology and Department of Neurology of the Second Affiliated Hospital NHC and CAMS Key Laboratory of Medical NeurobiologyZhejiang University School of MedicineHangzhou 310058China The MOE Frontier Science Center for Brain Research and Brain-Machine Integration School of Brain Science and Brain MedicineZhejiang UniversityHangzhou 310058China

Neurons are highly interwoven to form intricate neural circuits that underlie the diverse functions of the *** the anatomical organization of neural circuits is key to deciphering how the brain processes information,produces thoughts,and instructs *** the past decades,recombinant viral vectors have become the most commonly used tracing tools to define circuit *** this review,we introduce the current categories of viral tools and their proper application in circuit *** further discuss some advances in viral tracing strategy and prospective innovations of viral tools for future study.

关键词： viral tracing Neural circuit Anterograde Retrograde Transsynaptic

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Development of viral tools for neurocircuits

Development of viral tools for neurocircuits

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中国神经科学学会第十届全国学术会议

作者： Xiaobin HE Zhijian ZHANG Jia JIANG Sen JIN Tingting DING Fuqiang XU Key Laboratory of Magnetic Resonance in Biological System Chinese Academy of SciencesWuhan Institute of Physics and MathematicsCAS

Objective All our behaviors,from basic condition-controlling to higher functions,are based on neural circuits,and accordingly, "Mental illness is defined as disruption in neural circuits"（the National Institute of Mental Health,NIH,2013）.To elucidate the mechanism of a given brain function,we first have to know the exact structures of all circuits related to that ***,the studies at circuit level have been hindered by lacking of effective *** purpose is to develop tools to trace the neuronal circuits,and to apply these tools to reveal the detailed structure of neuronal *** By using molecular and cellular methods to replicate,modify and prepare viral tools and then apply these tools to different types of animals,including non-human primate,rat,mouse,zebra fish,and bat to demonstrate their *** Several known viral tracing systems have been successfully rephcated and used in several different laboratories for a variety of purposes;new systems have also been constructed and tested for their properties in *** viral tools are effective to reveal neuronal circuits.

关键词： viral tracing neuronal circuits different species.

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