Use of a tissue clearing technique combined with retrograde trans-synaptic viral tracing to evaluate changes in mouse retinorecipient brain regions following optic nerve crush

作     者：Zong-Yi Zhan Yi-Ru Huang Lu-Wei Zhao Ya-Dan Quan Zi-Jing Li Di-Fang Sun Ya-Li Wu Hao-Yuan Wu Zi-Tian Liu Kai-Li Wu Yu-Qing Lan Min-Bin Yu Zong-Yi Zhan;Yi-Ru Huang;Lu-Wei Zhao;Ya-Dan Quan;Zi-Jing Li;Di-Fang Sun;Ya-Li Wu;Hao-Yuan Wu;Zi-Tian Liu;Kai-Li Wu;Yu-Qing Lan;Min-Bin Yu

作者机构：Department of OphthalmologyGuangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene RegulationSun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina State Key Laboratory of OphthalmologyZhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Light Innovation Technology Ltd.Hong Kong Special Administrative RegionChina Department of Ophthalmology&Visual ScienceShanghai Medical CollegeFudan UniversityShanghaiChina Department of OphthalmologyEye&ENT HospitalFudan UniversityShanghaiChina Department of OphthalmologyThe First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2023年第18卷第4期

页      面：913-921页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China No.81870655(to MBY) 

主　　题：histology image analysis light-sheet imaging optic nerve crush pseudorabies virus retinal ganglion cells three-dimensional imaging tissue clearing viral tracing whole brain study 

摘      要：Successful establishment of reconnection between retinal ganglion cells and retinorecipient regions in the brain is critical to optic nerve ***,morphological assessments of retinorecipient regions are limited by the opacity of brain *** this study,we used an innovative tissue cleaning technique combined with retrograde trans-synaptic viral tracing to observe changes in retinorecipient regions connected to retinal ganglion cells in mice after optic nerve ***,we performed light-sheet imaging of whole brain tissue after a clearing *** found that pseudorabies virus 724(PRV724)mostly infected retinal ganglion cells,and that we could use it to retrogradely trace the retinorecipient regions in whole tissue-cleared ***,PRV724-traced neurons were more widely distributed compared with data from previous *** found that optic nerve injury could selectively modify projections from retinal ganglion cells in the hypothalamic paraventricular nucleus,intergeniculate leaflet,ventral lateral geniculate nucleus,central amygdala,basolateral amygdala,Edinger-Westphal nucleus,and oculomotor nucleus,but not the superior vestibular nucleus,red nucleus,locus coeruleus,gigantocellular reticular nucleus,or facial nerve *** findings demonstrate that the tissue clearing technique,combined with retrograde trans-synaptic viral tracing,can be used to objectively and comprehensively evaluate changes in mouse retinorecipient regions that receive projections from retinal ganglion cells after optic nerve ***,our approach may be useful for future estimations of optic nerve injury and regeneration.