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Triolein emulsion infusion into the hepatic artery increases vascular permeability to doxorubicin in rabbit liver

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World Journal of Gastroenterology 2021年第2期27卷 152-161页

作者： Yong-Woo Kim Hak Jin Kim Byung Mann Cho Seon Hee Choi Department of Radiology Research Institute for Convergence of Biomedical Science and TechnologyPusan National University Yangsan HospitalPusan National University College of MedicineYangsan 50612South Korea Department of Radiology Biomedical Research InstitutePusan National University HospitalPusan National University College of MedicineBusan 49241South Korea Preventive Medicine and Occupational Medicine Pusan National University School of MedicineYangsan 50612South Korea Department of Radiology Pusan National UniversityBusan 49241South Korea

BACKGROUND The infusion of triolein emulsion(TE)induced increased vascular permeability and a negligible and temporary decrease in liver function without specific histopathological *** To assess changes in doxorubicin concentration according to the percentage of TE infused via a hepatic artery to study the vascular permeability in the rabbit *** Thirty-nine healthy rabbits were divided into five groups according to the concentration of emulsified triolein infused into the hepatic arteries:Group 0,saline infusion(control group,n=5);group 1,0.3%TE(n=13);group 2,0.6%TE(n=6);group 3,0.9%TE(n=8);and group 4,1.5%TE(n=6).Doxorubicin(2.4 mg/kg)was infused immediately after TE injection via the hepatic *** 2 h,the livers were harvested,and doxorubicin concentrations were calculated *** doxorubicin concentrations were compared between TE groups and the control group,and the optimal concentrations within the TE groups were *** analysis was performed using the nonparametric Mann-Whitney U test and Kruskal-Wallis test.P0.05).CONCLUSION TE infusion into the hepatic arteries significantly increased the doxorubicin concentration approximately twofold but was not different between the TE *** findings suggest that TE infusion might be a useful adjuvant treatment of liver cancers.

关键词： Triolein emulsion Liver tumor Chemotherapy Drug delivery vascular permeability Dexorubicin

The correlation between lung vascular permeability and the expression of Cx43,Cx40 and OPN during septic shock in rats

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The correlation between lung vascular permeability and the e...

AB004. Regulation of retinal angiogenesis and vascular permeability by bone morphogenetic protein signaling

2012中国生理学会心血管生理学术研讨会

作者：张杰李涛刘良明 State Key Laboratory of Trauma Bums and Combined InjuryDepartment 2Institute of Surgery ResearchDaping HospitalThird Military Medical University

＜正＞Objective:The increase of vascular permeability is the main mechanism for vascular leakage during critical condition such as sepsis,septic shock,pancreatitis and multiple organ dysfunction *** previous study found that connexins（Cx40,Cx43） participated in the regulation of vascular reactivity following *** present study was aimed to explore the role of Cx40,Cx43 and Osteopontin（OPN）in the changes of

关键词： lung vascular permeability Cx43 Cx40 septic shock

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Annals of Eye Science 2018年第1期 410-410页

作者： Bruno Larrivée Department of Ophthalmology Hôpital Maisonneuve-Rosement Research CentreUniversitéde MontréalMontrealCanada

The bone morphogenetic protein(BMP)family of proteins has a multitude of roles throughout the *** plays important roles in development and in the adult vascular endothelium,by modulating the angiogenic *** endothelial-specific receptor BMP receptor Alk1 is of particular importance in the proper remodeling of the vasculature and its ligand BMP9 has been shown to be a potent inhibitor of *** BMP signaling has been linked to multiple vascular diseases and can lead to the abnormal *** therefore investigated the role of BMP9/Alk1 signaling in retinal angiogenesis,and its therapeutic implications for vascular pathologies of the eye.

关键词： Retinal angiogenesis bone morphogenetic protein(BMP) vascular permeability

AB039. BMP9 signaling maintains endothelial integrity and prevents hyperglycemia-induced retinal vascular permeability

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Annals of Eye Science 2019年第1期 214-214页

作者： Naoufal Akla Claire Viallard Natalija Popovic Cindy Lora Gil Przemyslaw Sapieha Bruno Larrivée Departement of Biochemistry and Molecular Medecine Universitéde MontréalMontréalQCCanada Centre de Recherche de l'Hôpital Maisonneuve-Rosemont MontréalQCCanada Departement of Molecular Biology Universitéde MontréalMontréalQCCanada Departement of Biomedical Sciences Universitéde MontréalMontréalQCCanada Departement of Ophtalmology Universitéde MontréalMontréalQCCanada

Background:The maintenance of a quiescent retinal vascular endothelial barrier is paramount for tissue supply and homeostasis to ensure visual *** hyperglycemia in diabetes causes structural and functional alterations of the endothelium that are accelerated by the production of several mediators such as *** disturbance of interendothelial junction stability leading to retinal hyperpermeability is one of the changes leading to diabetic macular edema(DME)that can occur at any stage of diabetic *** in our understanding of the pathophysiological mechanisms of DME have enabled effective new therapies such as anti-VEGF’s,which are however associated with non-negligible side *** discovery of endothelium-specific protective targets that could restore retinal endothelial quiescence could provide a therapeutic *** mediated by BMP9 circulating protein via its endothelium-specific receptor ALK1,is known for its role in the maintenance of vascular ***,its ability to protect the endothelium and prevent vascular permeability has not been tested in the context of ***:We investigated BMP9/ALK1 signalling pathway in the hyperglycemic endothelium and its effect on retinal permeability in a type 1 diabetes mouse *** endothelial cells and tissue were extracted to evaluate BMP9/ALK1 ***9 overexpression was achieved using adenoviral *** permeability was measured using miles ***:We found that BMP9/ALK1 signaling was inhibited in hyperglycemic endothelial cells and blood vessels of diabetic(DB)mice,and that this loss of function was directly associated with retinal ***,inhibition of this pathway triggers the activation of the VEGFR2/SRC pathway reducing interendothelial adhesion ***,the activation of ALK1 by sustained BMP9 overexpression in DB mice enabled the restoration of physiological permeability by regulatin

关键词： Diabetes vascular permeability activin receptor

Pericytes protect rats and mice from sepsis-induced injuries by maintaining vascular reactivity and barrier function:implication of miRNAs and microvesicles

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Military Medical Research 2024年第1期11卷 1-18页

作者： Zi-Sen Zhang Yi-Yan Liu Shuang-Shuang He Dai-Qin Bao Hong-Chen Wang Jie Zhang Xiao-Yong Peng Jia-Tao Zang Yu Zhu Yue Wu Qing-Hui Li Tao Li Liang-Ming Liu State Key Laboratory of Trauma Burns and Combined InjuryDepartment of Shock and TransfusionResearch Institute of SurgeryDaping HospitalArmy Medical UniversityChongqing 400042China

Background vascular hyporeactivity and leakage are key pathophysiologic features that produce multi-organ damage upon *** hypothesized that pericytes,a group of pluripotent cells that maintain vascular integrity and tension,are protective against sepsis via regulating vascular reactivity and *** We conducted a series of in vivo experiments using wild-type(WT),platelet-derived growth factor receptor-β(PDGFR-β)-Cre+mT/mG transgenic mice and Tie2-Cre+Cx43^(flox/flox)mice to examine the relative contribution of pericytes in sepsis,either induced by cecal ligation and puncture(CLP)or lipopolysaccharide(LPS)*** a separate set of experiments with Sprague-Dawley(SD)rats,pericytes were depleted using CP-673451,a selective PDGFR-βinhibitor,at a dosage of 40 mg/(kg·d)for 7 consecutive *** pericytes,vascular endothelial cells(VECs)and vascular smooth muscle cells(VSMCs)were used for mechanistic *** effects of pericytes and pericyte-derived microvesicles(PCMVs)and candidate miRNAs on vascular reactivity and barrier function were also *** CLP and LPS induced severe injury/loss of pericytes,vascular hyporeactivity and leakage(Pvascular reactivity and barrier function via microvessel colonization(Pvascular reactivity and barrier function after sepsis(Pvascular endothelial-cadherin *** Pericytes are protective a

关键词： Pericyte vascular reactivity vascular permeability Cx43 Microvesicle

Knockdown of fibrillin-1 suppresses retina-blood barrier dysfunction by inhibiting vascular endothelial apoptosis under diabetic conditions

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International Journal of Ophthalmology(English edition) 2024年第8期17卷 1403-1410页

作者： Yue Zhang Xiao-Jing Liu Xin-Ran Zhai Yao Yao Bin Shao Yu-Han Zhen Xin Zhang Zhe Xiao Li-Fang Wang Ming-Lian Zhang Zhi-Min Chen Hebei Eye Hospital Provincial Key Laboratory of OphthalmologyHebei Provincial Clinical Research Center for Eye DiseasesXingtai 054001Hebei ProvinceChina Department of Ophthalmology Hebei Medical UniversityShijiazhuang 050011Hebei ProvinceChina

AIM:To investigate the effects of fibrillin-1(FBN1)deletion on the integrity of retina-blood barrier function and the apoptosis of vascular endothelial cells under diabetic ***:Streptozotocin(STZ)-induced diabetic mice were used to simulate the diabetic conditions of diabetic retinopathy(DR)patients,and FBN1 expression was detected in retinas from STZ-diabetic mice and *** the Gene Expression Omnibus(GEO)database,the GSE60436 dataset was selected to analyze FBN1 expressions in fibrovascular membranes from DR *** lentivirus to knock down FBN1 levels,vascular leakage and endothelial barrier integrity were detected by Evans blue vascular permeability assay,fluorescein fundus angiography(FFA)and immunofluorescence labeled with tight junction marker in *** glucose-induced monkey retinal vascular endothelial cells(RF/6A)were used to investigate effects of FBN1 on the cells in *** vascular endothelial barrier integrity and apoptosis were detected by trans-endothelial electrical resistance(TEER)assay and flow cytometry,***:FBN1 mRNA expression was increased in retinas of STZ-induced diabetic mice and fibrovascular membranes of DR patients(GSE60436 datasets)using RNA-seq ***,knocking down of FBN1 by lentivirus intravitreal injection significantly inhibited the vascular leakage compared to STZ-DR group by Evans blue vascular permeability assay and FFA *** of tight junction markers in STZ-DR mouse retinas were lower than those in the control group,and knocking down of FBN1 increased the tight junction *** vitro,30 mmol/L glucose could significantly inhibit viability of RF/6A cells,and FBN1 mRNA expression was increased under 30 mmol/L glucose ***-regulation of FBN1 reduced high glucose(HG)-stimulated retinal microvascular endothelial cell permeability,increased TEER,and inhibited RF/6A cell apoptosis in ***:The expression level of FBN1 increases in reti

关键词： diabetic retinopathy fibrillin-1 retinablood barrier vascular leakage vascular permeability apoptosis retinal vascular endothelial cells

Ginkgo biloba extract(EGb 761) attenuates lung injury induced by intestinal ischemia/reperfusion in rats:Roles of oxidative stress and nitric oxide

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World Journal of Gastroenterology 2007年第2期13卷 299-305页

作者： Ke-Xuan Liu Wei-Kang Wu Wei He Chui-Liang Liu Department of Anesthesiology The First Affiliated HospitalSun Yat-Sen University Guangzhou 510080 Guangdong Province China The Institute of Integrated Traditional Chinese Medicine and Western Medicine Sun Yat-Sen University Guangzhou 510080 Guangdong Province China Department of Anesthesiology Guangdong Provincial People's Hospital Guangzhou 510080 Guangdong Province China Department of Anesthesiology Guangdong Provincial.Hospital of Traditional Chinese Medicine Guangzhou 510120 Guangdong Province China

AIM： To investigate the effect of ginkgo biloba extract （EGb 761） on lung injury induced by intestinal ischemia/ reperfusion （ Ⅱ/R）. METHODS： The rat model of Ⅱ/R injury was produced by damping the superior mesenteric artery for 60 min followed by reperfusion for 180 min. The rats were randomly allocated into sham, Ⅱ/R, and EGb ＋Ⅱ/R groups. In EGb ＋Ⅱ/R group, EGb 761 （100 mg/kg per day） was given via a gastric tube for 7 consecutive days prior to surgery. Rats in Ⅱ/R and sham groups were treated with equal volumes of the vehicle of EGb 761. Lung injury was assessed by light microscopy, wet-todry lung weight ratio （W/D） and pulmonary permeability index （PPT）. The levels of malondialdehyde （MDA） and nitrite/nitrate （NO2/NO3）, as well as the activities of superoxide dismutase （SOD） and myeloperoxidase （MPO） were examined. Western blot was used to determine the expression of inducible nitric oxide synthase （iNOS）. RESULTS： EGb 761 markedly improved mean arterial pressure and attenuated lung injury, manifested by the improvement of histological changes and significant decreases of pulmonary W/D and PPT （P 〈 0.05 or 0.01）.Moreover, EGb 761 markedly increased SOD activity, reduced MDA levels and MPO activity, and suppressed NO generation accompanied by down-regulation of iNOS expression （P 〈 0.05 or 0.01）. CONCLUSION： The results indicate that EGb 761 has a protective effect on lung injury induced by Ⅱ /R, which may be related to its antioxidant property and suppressions of neutrophil accumulation and iNOS- induced NO generation. EGb 761 seems to be an effective therapeutic agent for critically ill patients with respiratory failure related to Ⅱ/R.

关键词： Ginkgo biloba Extract Intestine Reperfusion injury Lung Adult respiratory distress syndrome vascular permeability Nitric oxide Lipid peroxidation

Pretreatment with scutellaria baicalensis stem-leaf total flavonoid protects against cerebral ischemia/reperfusion injury in hippocampal neurons

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Neural Regeneration Research 2014年第23期9卷 2066-2073页

作者： Xiangyu Kong Wei Kong Guangxin Miao Shumin Zhao Meng Chen Xiaoying Zheng Jiangtao Bai Laboratory of Spinal Cord Injury and Rehabilitation Chengde Medical College

Previous experimental studies have shown that cerebral infarction can be effectively reduced following treatment with scutellaria baicalensis stem-leaf total flavonoid （SSTF）. However, the mechanism of action of SSTF as a preventive drug to treat cerebral infarction remains unclear. In this study, Sprague-Dawley rats were pretreated with 50, 100, 200 mg/kg SSTF via intragastric ad- ministration for 1 week prior to the establishment of focal cerebral ischemia/reperfusion injury. The results showed that pretreatment with SSTF effectively improved neurological function, reduced brain water content and the permeability of blood vessels, ameliorated ischemia-induced morphology changes in hippocampal microvessels, down-regulated Fas and FasL protein expression, elevated the activity of superoxide dismutase and glutathione peroxidase, and decreased malondialdehyde content. In contrast to low-dose SSTF pretreatment, the above changes were most obvious after pretreatment with moderateand high-doses of SSTF. Experimental findings indicate that SSTF pretreatment can exert protective effects on the brain against cerebral ischemia/reperfusion injury. The underlying mechanisms may involve reducing brain water content, increasing microvascular recanalization, inhibiting the apoptosis of hippocampal neurons, and attenuating free radical damage.

关键词： nerve regeneration scutellaria baicalensis stem-leaf total flavonoid pretreatment cerebral ischemia/reperfusion hippocampus apoptosis vascular permeability free radicals neural regeneration

Protective effects of dl-3n-butylphthalide against diffuse brain injury

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Neural Regeneration Research 2013年第28期8卷 2615-2624页

作者： Yaning Zhao Jianmin Li Pan Zhang Changxiang Chen Shuxing Li College of Rehabilitation Hebei United University Affiliated Hospital of Hebei United University

DI-3n-butyiphthalide can effectively treat cerebral ischemia; however, the mechanisms underlying the effects of dl-3n-butylphthalide on microcirculation disorders following diffuse brain injury remain unclear. In this study, models of diffuse brain injury were established in Sprague-Dawley rats with the vertical impact method. DI-3n-butylphthalide at 80 and 160 mg/kg was given via intraperitoneal injection immediately after diffuse brain injury. Ultrastructural changes in the cerebral cortex were observed using electron microscopy. Cerebral blood flow was measured by laser Doppler flowmetry, vascular density was marked by tannic acid-ferric chloride staining, vascular permeability was es- timated by the Evans blue method, brain water content was measured using the dry-wet method, and rat behavior was measured by motor function and sensory function tests. At 6, 24, 48, and 72 hours after administration of dl-3n-butylphthalide, reduced cerebral ultrastructure damage, in- creased vascular density and cerebral blood flow, and improved motor and sensory functions were observed. Our findings demonstrate that dl-3n-butylphthalide may have protective effects against diffuse brain injury by ameliorating microcirculation disorder and reducing blood-brain barrier dam- age and cerebral edema.

关键词： neural regeneration brain injury diffuse brain injury blood-brain barrier brain edema vasculardensity cerebral blood flow vascular permeability brain water content grants-supported paper neuroregeneration