Ginkgo biloba extract(EGb 761) attenuates lung injury induced by intestinal ischemia/reperfusion in rats:Roles of oxidative stress and nitric oxide

作     者：Ke-Xuan Liu Wei-Kang Wu Wei He Chui-Liang Liu 

作者机构：Department of Anesthesiology The First Affiliated HospitalSun Yat-Sen University Guangzhou 510080 Guangdong Province China The Institute of Integrated Traditional Chinese Medicine and Western Medicine Sun Yat-Sen University Guangzhou 510080 Guangdong Province China Department of Anesthesiology Guangdong Provincial People's Hospital Guangzhou 510080 Guangdong Province China Department of Anesthesiology Guangdong Provincial.Hospital of Traditional Chinese Medicine Guangzhou 510120 Guangdong Province China 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2007年第13卷第2期

页      面：299-305页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by grants from the Administration of Traditional Chinese Medicine of Guangdong Province, China, No. 1040066 Natural Science Foundation of Guangdong Province, China, No. 05300758 National Natural Science Foundation of China, No. 30672021 

主　　题：Ginkgo biloba Extract Intestine Reperfusion injury Lung Adult respiratory distress syndrome Vascular permeability Nitric oxide Lipid peroxidation 

摘      要：AIM： To investigate the effect of ginkgo biloba extract （EGb 761） on lung injury induced by intestinal ischemia/ reperfusion （ Ⅱ/R）. METHODS： The rat model of Ⅱ/R injury was produced by damping the superior mesenteric artery for 60 min followed by reperfusion for 180 min. The rats were randomly allocated into sham, Ⅱ/R, and EGb ＋Ⅱ/R groups. In EGb ＋Ⅱ/R group, EGb 761 （100 mg/kg per day） was given via a gastric tube for 7 consecutive days prior to surgery. Rats in Ⅱ/R and sham groups were treated with equal volumes of the vehicle of EGb 761. Lung injury was assessed by light microscopy, wet-todry lung weight ratio （W/D） and pulmonary permeability index （PPT）. The levels of malondialdehyde （MDA） and nitrite/nitrate （NO2/NO3）, as well as the activities of superoxide dismutase （SOD） and myeloperoxidase （MPO） were examined. Western blot was used to determine the expression of inducible nitric oxide synthase （iNOS）. RESULTS： EGb 761 markedly improved mean arterial pressure and attenuated lung injury, manifested by the improvement of histological changes and significant decreases of pulmonary W/D and PPT （P 〈 0.05 or 0.01）.Moreover, EGb 761 markedly increased SOD activity, reduced MDA levels and MPO activity, and suppressed NO generation accompanied by down-regulation of iNOS expression （P 〈 0.05 or 0.01）. CONCLUSION： The results indicate that EGb 761 has a protective effect on lung injury induced by Ⅱ /R, which may be related to its antioxidant property and suppressions of neutrophil accumulation and iNOS- induced NO generation. EGb 761 seems to be an effective therapeutic agent for critically ill patients with respiratory failure related to Ⅱ/R.