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Neural Regeneration Research 2017年第4期12卷 584-585页

作者： Moeen Riaz Paul N. Baird Centre for Eye Research Australia Department of Surgery (Ophthalmology) University of MelbourneRoyal Victorian Eye and Ear Hospital

Age related macular degeneration （AMD） is a complex progres- sive neurodegenerative disease causing blindness in 30-35 million people worldwide. It affects the macula region of the retina leading to severe vision loss and legal blindness in individuals 〉 50 years of age （Wong et al., 2014）. The precise aetiology of AMD is unknown but smoking, age and genetic factors are major risk factors for AMD predisposition （Ding et al., 2009）. The genetic basis of AMD is well described with a recent study from the International AMD gene consortium （IAMDGC） reporting 52 genetic variants across 34 loci associated with the risk of AMD pathogenesis and explaining more than 50% of the genetic heritabilitv of the disease （Fritsche et al., 2016）.

关键词： vegf Recent advances and future directions for the pharmacogenetic basis of anti-vegf treatment response in neovascular age-related macular degeneration

Expression and significance of vegf and p53 in rat degenerated intervertebral disc tissues

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Asian Pacific Journal of Tropical Medicine 2013年第5期6卷 404-406页

作者： Xiao-Wei Liu Jin Kang Xian-Dong Fan Li-Feng Sun Orthopedics Center No.251 Hospital of People's Liberation Army

Objective:To discuss the expression and significance of vascular endothelial growth factor (vegf) and p53 expression in rat degenerated intervertebral disc(IVD) ***:A total of 78 even-aged Sprague-Dawley(SD) rats(observation group) were chosen to establish a rat IVD degeneration model with anterior limbs and tail-removing *** months after modeling, rats were executed and degenerated IVD tissues were obtained for pathological biopsy so as to observe vegf and p53 expression ***,IVD tissues from 8 healthy rats were gained for ***:Capillary infiltration was found in 62.8%of cases in observation group. Positive expression of vegf and p53 was seen in 74.4%and 59.0%of cases respectively with a co-expression rate of 53.8%.VECF and p53 expression in degenerated IVD tissues with capillary infiltration was higher than that in non-infiltration group(Pvegf and p53 in rat degenerated IVD tissues is demonstrated,indicating that they are both involved in neovascularization and infiltration,which accelerates IVD degeneration.

关键词： Intervertebral disc Neovascularization Immunohistochemistry vegf p53 protein

Effect of endostar combined with cisplatin on expression of vegf and Sema3A of Lewis lung cancer rats

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Asian Pacific Journal of Tropical Medicine 2013年第1期6卷 57-60页

作者： Ping Feng Zhi-Lin Zhang Zhi-Hua Zhang Xiu-Long Zhang Feng Xiang Jian-Hua Tang Bao-Li Xiang Master of Respiratory Medicine Hebei Northern College Department of Respiratory Medicine First Affiliated Hospital of Hebei Northern College Department of Clinical Pharmacology First Affiliated Hospital of Hebei Northern College

Objective:To evaluate the therapeutic effect of endostar(ED) combined with cisplatin(DDP) on model of C57BL/6 rats,and to further investigate the inhibiting mechanism of endostar from tumor ***:Lewis lung cancer cells were inoculated in C57BL/6 mouse,then the mouse were randomized into control group(group A),ED(group B),DDP(group C) and ED/DDP (group D).They were treated according to the *** the expressions of vegf and Sema3A were evaluated by ***:The weight of tumor increased in group A and *** was decreased in group C and *** tumor volume was increased in all the 4 groups. The vegf expression of group D was obviously lower than the other group 3,but the Sema3A expressed of group D was significantly strengthener than the other group *** vegf expression of group B and group D were obviously low especially in the 4th-8th *** correlated analysis showed that the expression vegf and Sema3A were negatively correlated(r=-0.72, Pvegf expression,and enhance Sema3A *** vessel presents transient *** is easy for DDP perfusion,and to kill tumor cells.

关键词： Lewis lung cancer Endostar DDP vegf Sema3A

Expression and significance of vegf and p53 in degenerate intervertebral disc tissue

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Asian Pacific Journal of Tropical Medicine 2013年第1期6卷 79-81页

作者： Xiao-Yu Lu Xiao-Hong Ding Li-Jun Zhong Hong Xia Xiao-Dong Chen Hai Huang Rehabilitation Medicine Hospital of Special SurgeryGuangzhou General Hospital of Guangzhou Military Command 1st Department of Spine Guangzhou General Hospital of Guangzhou Military Command Pathology Department Guangzhou General Hospital of Guangzhou Military Command

Objective:To investigate the mechanism of expression and significance of vascular endothelial growth factor(vegf) and p53 in degenerate intervertebral disc ***:Pathological sections collected from 156 patienls with lumbar disc herniation after surgery were tested by immunohistochemistry method,for evaluation of the expression of vegf and p53 in degenerate intervertebral disc ***:98 cases(62.8%) with vascular infiltration phenomenon are found,and positive rates of vegf and p53 in degenerate intervertebral disc tissue are 73.42% (116/156) and 58.97%(92/156);co-expression rate is 53.2%(83/l56);the expression rates of VEFG and p53 are significantly higher in the tissue with blood vessel infiltration than in the tissue without infiltration;there is a close relationship of vegf with ***:vegf and p53 gene synergetic express in degenerate intervertebral disc tissue,working together in neovascularization and infiltration,and accelerating intervertebral disc tissue degeneration.

关键词： Intervertebral disc Immunohistochemistry vegf p53

Transcriptional regulation of vegf expression by estrogen-related receptor γ

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Acta Pharmaceutica Sinica B 2013年第6期3卷 373-380页

作者： Jichao Liang Fengmei Han Yong Chen Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine Hubei UniversityWuhan 430062China

Vascular endothelial growth factor(vegf)is associated with endothelial cell proliferation,migration and ***-related receptorγ(ERRγ)plays important regulatory roles in fatty acid oxidation,mitochondrial biogenesis and ***,ERRγ has been shown to be involved in angiogenesis;however,the mechanism of ERRγ-mediated angiogenesis is poorly *** the results show that ERRγactivates vegf gene transcription via two putative estrogen-related receptor binding elements(ERREs)mapped to the promoter *** immunoprecipitation assays confirmed that ERRγ binds to the identified ***-mediated overexpression of ERRγ increased the expression of vegf gene and induced an increase in vegf secretion in C2C12 *** contrast,the secretion of vegf was significantly decreased in the presence of the ERRγ inverse agonist ***,treatment of human umbilical vein endothelial cells(HUVECs)with the conditioned medium from ERRγ-overexpressing C2C12 myotubes significantly increased proliferation,migration and tube *** data indicate that vegf functions as a downstream target gene of ERRγ and mediates the effects of ERRγ on endothelial cells proliferation,migration and *** physiological function of ERRγ might provide a novel target for treatment of ischemic diseases.

关键词： Estrogen-related receptorγ vegf Promoter Transcription Migration

Overexpression of ACE2 ameliorates Aβ-induced blood–brain barrier damage and angiogenesis by inhibiting NF-κB/vegf/vegfR2 pathway

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Animal Models and Experimental Medicine 2023年第3期6卷 237-244页

作者： Xueling Zhang Yu Zhang Ling Zhang Chuan Qin Institute of Laboratory Animal Sciences CAMS&PUMCBeijingChina National Human Diseases Animal Model Resource Center BeijingChina NHC Key Laboratory of Human Disease Comparative Medicine BeijingChina Changping National Laboratory(CPNL) BeijingChina

Background:Pathological angiogenesis and blood–brain barrier damage may play an important role in Alzheimer's disease(AD).ACE2 is mainly expressed on the surface of endothelial cells in *** studies have shown that the expression of ACE2 in AD is reduced,but its role in AD is still ***:We induced AD damage in endothelial cells using Aβ25-35 and overexpressed ACE2 in bEend.3 cells through lentiviral *** detected the effect of Aβ25-35 on cell viability using the CCK-8 assay and examined the effect of overexpressing ACE2 on angiogenesis using an angiogenesis *** used western blot and cell immunofluorescence to detect changes in the expression of the vegf/vegfR2 pathway,tight junction protein,and NF-κB ***:Aβ25-35 treatment significantly decreased the expression of ACE2 and reduced cell ***2 overexpression(1)reduced the number of branches and junctions in tube formation,(2)inhibited the activation of the vegf/vegfR2 pathway induced by Aβ25-35,(3)increased the expression of TJPs,including ZO-1 and claudin-5,and(4)restored Aβ25-35-induced activation of the NF-κB ***:Overexpression of ACE2 can improve pathological angiogenesis and blood–brain barrier damage in AD models in vitro by inhibiting NF-κB/vegf/vegfR2 pathway ***2 may therefore represent a therapeutic target for endothelial cell dysfunction in AD.

关键词： ACE2 Alzheimer's disease angiogenesis blood-brain barrier vegf

Homoharringtonine induces apoptosis of endothelium and down－regulates vegf expression of K562 cells

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Journal of Zhejiang University Science 2004年第2期5卷 230-234页

作者：叶琇锦林茂芳 DepartmentofHematology theFirstAffiliatedHospitalMedicalCollegeZhejiangUniversityHangzhou310003China

Homoharringtonine (HHT) has currently been used successfully in the treatment of acute and chronic myeloid leukemias and has been shown to induce apoptosis of different types of leukemic cells in vitro. Emerging evidence suggests that angiogenesis may play an important role in hematological malignancies, such as leukemia. However, whether HHT can relieve leukemia by anti-angiogenesis is still unknown. We investigated the anti-angiogenesis potential of HHT with the human umbilical vein endothelial cell line (ECV304) and leukemic cell line (K562) in vitro. Cellular proliferation was determined by MTT assay and apoptosis was analyzed by flow cytometry, The mRNA expression of vascular endothelial growth factor (vegf) was assessed by RT-PCR and vegf protein production was detected by Western blot. Inhibition of cell proliferation and induction of apoptosis by HHT were discovered in ECV304 cells, and appeared in a dose- and time-dependent manner, Also, treatment with HHT caused down-regulation of vegf mRNA expression in K562 cells in similar dose- and time-dependent manner and inhibition of vegf protein production in K562 cells in response to the enhancing concentration of HHT. The results demonstrated that HHT could also induce apoptosis in endothelium and down-regulate vegf expression in K562 cells. In conclusion, we believe HHT has anti-angiogenesis potential and speculate that HHT might exert its anti-leukemia effects via reduction of angiogenesis.

关键词： Homoharringtonine (HHT) Leukemia Angiogenesis vegf

Effect of down-regulating vegf on proliferation of colon carcinoma cell HT-29

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Frontiers in Biology 2007年第1期2卷 35-38页

作者： ZHANG Xiao GE Yinlin WANG Hongwei CAO Mingzhi ZHANG Jinyu ZHANG Zheng WANG Xiuli Department of Biochemistry and Molecular Biology Medical CollegeQingdao UniversityQingdao 266021China Clinical Laboratory of Affiliated Hospital Medical CollegeQingdao UniversityQingdao 266003China Oncosurgery Department of Affiliated Hospital Medical CollegeQingdao UniversityQingdao 266003China

We designed specific small interfering RNA(siRNA)targeting vascular endothelial growth factor(vegf)mRNA and synthesized oligo fragments,then siRNA was obtained by in vitro transcription and transfected into cultured human colon carcinoma cell line HT-29 with *** also analyzed the effect of the siRNA on proliferation of HT-29 cells by methyl thiazolyl tetrazolium(MTT)assay and expression level of vegf mRNA of transfected cells by RT-PCR as well as amounts of secreted vegf protein in the supernatant by enzyme linked immunosorbent assay(ELISA).Two groups of siRNA targeting human vegf effectively inhibited proliferation of HT-29 cells after *** secretion of vegf protein also notably decreased,but the control scramble siRNA showed no effect.

关键词： siRNA HT-29 vegf gene therapy

Effect of tetramethylpyrazin combined with cisplatin on vegf,KLF4 and ADAMTS1 in lewis lung cancer mice

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Asian Pacific Journal of Tropical Medicine 2017年第8期10卷 874-878页

作者： Jian-Hua Tang He-Min Zhang Zhi-Hua Zhang Xiu-Long Zhang Department of Clinical Pharmacology First Affiliated Hospital of Hebei Northern CollegeZhangjiakou 075000China Department of Respiratory Medicine First Affiliated Hospital of Hebei Northern CollegeZhangjiakou China

Objective: To further explore the function of combine use of tetramethylpyrazine(TMP) and cisplatin(DDP) in lung carcinoma. Methods: We used the combination drug to treat Lewis lung cancer mice, investigated the expression level of vascular endothelial growth factor(vegf), Kruppel-like factor 4(KLF4) and A disintegrin and metalloproteinase with thrombospondin motifs 1(ADAMTS1) and to further explore the inhibitory effects and potential mechanism of TMP combined with DDP on tumor angiogenesis. Results: The tumor growth was suppressed in TMP group, DDP group and TMP combined with DDP group. Furthermore, the weights and volume of tumor, the expression level of vegf, KLF4 and ADAMTS1 were found significantly changed between experiment group and control group. These findings suggest that TMP with DDP had additional or synergistic effects to inhibit the tumor growth effectively, might be achieved through reducing the expression of angiogenesis promoting factor vegf and increasing expression of angiogenesis inhibitors KLF4 and ADAMTS1. Conclusion: KLF4 and ADAMTS1 may be synergically involved in the angiogenesis in mouse Lewis lung cancer through the different signal ways.

关键词： Non-small cell lung cancer Tetramethylpyrazine Cisplatin ADAMTS1 vegf KLF4

Paeoniflorin protects HUVECs against AOPP-induced oxidative injury by blocking ROS-HIF-1α/vegf pathway

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中国药理学通报 2015年第B11期31卷 140-141页

作者： YANGXi-xiao SONGShao-lian MoLi-qian XIAOXiao-yan DeptofPharmacy NanfangHospitalShouthernMedicalUniversityGuangzhouGuangdong510515

Advanced oxidation protein products （AOPPs）, as a novel indicator of oxidative stress, are thought to be involved in aging-related diseases. The excessive AOPPs were served as independent risk factor for coronary artery disease （CAD）, atherosclerosis and carotid intima media thickness, which mainly through hypoxia inducible factor- l OL （HIF-Iot） and vascular endothelial growth factor （vegf） pathway. Paeoniflorin, a monoterpene glycoside, ex- erts well protective effect in vascular as its good antioxidant property. However, there is no research that has reported whether Paeoniflorin has the protective effect on oxidative damage induced by AOPPs in HUVECs, and also it is little known about this underlying mechanism. The protective effect of Paeoniflorin on oxidative damage induced by AOPPs was investigated in HUVECs. The cell viability was assessed by MTT colorimetric assay. The fluorescence intensity of 2＇, 7＇-dichlorofluorescein-diacetate （DCFH-DA） staining was detected for intracellular reactive oxygen species （ROS） production. And mitochondrial membrane potential （MMP） was measured byflow cytometry and confocal mi- croscopy staining with Mito Tracker Deep Red/ MitoTracker Green. The intracellular adenosine triphosphate （ATP） was measured by ATP Determination Kit. Nox2, Nox4, HIF-lα, vegf and NF-KB p65 protein expressions were detected by western blot. The results showed that AOPPs significantly decreased MMP and ATP in a dose-de- pendent manner. Furthermore, AOPPs increased HW-1α, vegf protein expression, and also partly increased NF-KB p65 expression may through increase of ROS production by up-regulations of Nox2, Nox4 and RAGE expression in HUVECs. These effects were remarkably reversed by pre-treatment of Paeoniflorin, which indicated that Paeoniflorin inhibited Nox2/Nox4 expression, restored ATP depletion and mitochondria dysfunction via ROS suppression, and down-regulated HIF-lα/vegf possibly via ROS-NF-KB axis. In conclusion, these results suggesting that

关键词： Paeoniflorin AOPPs reactive oxygen species RAGE NF-KB HIF-lα vegf