Effect of down-regulating VEGF on proliferation of colon carcinoma cell HT-29

作     者：ZHANG Xiao GE Yinlin WANG Hongwei CAO Mingzhi ZHANG Jinyu ZHANG Zheng WANG Xiuli 

作者机构：Department of Biochemistry and Molecular BiologyMedical CollegeQingdao UniversityQingdao 266021China Clinical Laboratory of Affiliated HospitalMedical CollegeQingdao UniversityQingdao 266003China Oncosurgery Department of Affiliated HospitalMedical CollegeQingdao UniversityQingdao 266003China 

出 版 物：《Frontiers in Biology》 (生物学前沿（英文版）)

年 卷 期：2007年第2卷第1期

页      面：35-38页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100214[医学-肿瘤学] 0836[工学-生物工程] 0713[理学-生态学] 10[医学] 

基　　金：This work was supported by Qingdao Science and Technology Project(No.03-1-YN-14) 

主　　题：siRNA HT-29 VEGF gene therapy 

摘      要：We designed specific small interfering RNA(siRNA)targeting vascular endothelial growth factor(VEGF)mRNA and synthesized oligo fragments,then siRNA was obtained by in vitro transcription and transfected into cultured human colon carcinoma cell line HT-29 with *** also analyzed the effect of the siRNA on proliferation of HT-29 cells by methyl thiazolyl tetrazolium(MTT)assay and expression level of VEGF mRNA of transfected cells by RT-PCR as well as amounts of secreted VEGF protein in the supernatant by enzyme linked immunosorbent assay(ELISA).Two groups of siRNA targeting human VEGF effectively inhibited proliferation of HT-29 cells after *** secretion of VEGF protein also notably decreased,but the control scramble siRNA showed no effect.