检索结果-南通市图书馆

Disruption of colonic barrier function and induction of mediator release by strains of Campylobacter jejuni that invade epithelial cells

引用

World Journal of Gastroenterology 2008年第48期14卷 7345-7352页

作者： Johannes Beltinger Jo del Buono Maeve M Skelly John Thornley Robin C Spiller William A Stack Christopher J Hawkey Wolfson Digestive Diseases Centre Nottingham University Hospital QMC Campus

AIM: To study the mechanisms by which Campylobacter jejuni (C. jejuni) causes inflammation and diarrhea. In particular, direct interactions with intestinal epithelial cells and effects on barrier function are poorly under- stood. METHODS: To model the initial pathogenic effects of C. jejuni on intestinal epithelium, polarized human colonic HCA-7 monolayers were grown on permeabilized filters and infected apically with clinical isolates of C. jejuni. Integrity of the monolayer was monitored by changes in monolayer resistance, release of lactate dehydrogenase, mannitol fluxes and electron microscopy. Invasion of HCA-7 cells was assessed by a modified gentamicin protection assay, translocation by counting colony forming units in the basal chamber, stimulation of mediator release by immunoassays and secretory responses in monolayers stimulated by bradykinin in an ussing chamber. RESULTS: All strains translocated across monolayers but only a minority invaded HCA-7 cells. Strains that invaded HCA-7 cells destroyed monolayer resistance over 6 h, accompanied by increased release of lactate dehydrogenase, a four-fold increase in permeability to [3H] mannitol, and ultrastructural disruption of tight junctions, with rounding and lifting of cells off the filter membrane. Synthesis of interleukin (IL)-8 and prostaglandin E2 was increased with strains that invaded the monolayer but not with those that did not. CONCLUSION: These data demonstrate two distinct effects of C. jejuni on colonic epithelial cells and provide an informative model for further investigation of initial host cell responses to C. jejuni.

关键词： Campylobacter jejuni Cell invasion Cellculture Chloride secretion Colonocyte HCA-7 cells Membrane permeability Monolayer Mucosal barrier ussing chamber

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Increased paracellular permeability of tumor-adjacent areas in 1,2-dimethylhydrazine-induced colon carcinogenesis in rats

引用

Cancer Biology & Medicine 2018年第3期15卷 251-259页

作者： Viktoria V.Bekusova Evgeny L.Falchuk Larisa S.Okorokova Natalia M.Kruglova AlexANDer D.Nozdrachev AlexANDer G.Markov Department of Physiology St.Petersburg State University I.P.Pavlov Institute of Physiology Russian Academy of Sciences

Objective: The morphology and functions of the proximal and distal large intestine are not the same. The incidence of colorectal cancer in these regions is also different, as tumors more often appear in the descending colon than in the ascending *** bowel disease and colorectal cancer can increase transepithelial permeability, which is a sign of reduced intestinal barrier function. However, there is not enough evidence to establish a connection between the difference in colorectal cancer incidence in the proximal and distal colon and intestinal permeability or the effects of carcinogenesis on the barrier properties in various areas of the colon. The aim of the study was to assess the permeability of different segments of the large intestine according to a developed mapping methodology in healthy rats and rats with 1,2-dimethylhydrazine(DMH)-induced colon ***: The short circuit current, the transepithelial electrical resistance and the paracellular permeability to fluorescein of large intestine wall of male Wistar rats were examined in the ussing chambers. The optical density of the solution from the serosa side to assess the concentration of the diffused fluorescein from mucosa to serosa was analyzed by spectrophotometry. The morphometric and histological studies were performed by optical ***: Rats with DMH-induced colon adenocarcinomas showed elevated transepithelial electrical resistance in the areas of neoplasm development. In contrast, there was no change in the electrophysiological properties of tumor adjacent areas, however,the paracellular permeability of these areas to fluorescein was increased compared to the control rats and was characterized by sharply reduced barrier ***: The barrier properties of the colon vary depending on tumor location. The tumors were less permeable than the intact intestinal wall and probably have a negative influence on tumor-adjacent tissues by disrupting their bar

关键词： Rat 1,2-dimethylhydrazine colorectal cancer ussing chamber short circuit current transepithelial electrical resistance intestinal permeability

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Enzyme activity and phosphate uptake in the small intestine of Sprague Dawley rats improved by supplementation of infant formula with prebiotics

引用

Animal Nutrition 2018年第3期4卷 300-304页

作者： Shuiyue Zhou Yuanxin Hang Jianwu Wang Rejun Fang College of Animal Science and Technology Hunan Agricultural University Xiangya School of Public Health Central South University

This study was to identify the effects of prebiotics supplemented in infant formula on enzyme activity and phosphate uptake in the small intestine of Sprague Dawley(SD) rats. Forty-eight healthy SD rats at15 days old(a week before weaning) with similar weight were randomly divided into 3 groups: A(control group), B, C, with 16 rats per group. Rats in groups A, B, C were fed a standard infant formula, the standard infant formula supplemented with oligosaccharides, and the standard infant formula supplemented with polysaccharides, respectively. The feeding test was conducted for 28 d. Compared with group A, the results showed the following: 1) the activities of sucrose and lactase in the small intestine were significantly increased in SD rats of group C(P < 0.05); 2) the relative expressions of lactase gene in the anterior and posterior segments of the small intestine were significantly increased by 1.68 and 2.26 in SD rats of group C(P < 0.05), and the relative expression of Mgam gene in the posterior segment of the small intestine was significantly increased by 0.99 in SD rats of group C(P < 0.05); 3) the relative expressions of Na/Pi-IIb gene in the anterior and posterior segments of the small intestine were significantly increased by 1.85 and 2.28 in SD rats of group C(P < 0.05). These results indicate that the supplementation of infant formula with prebiotics can promote enzyme activity in the small intestine by increasing the relative expression of enzyme gene or by decreasing intestinal injury, and can increase the relative expression of Na/Pi-IIb gene. The effect of polysaccharides is better than that of oligosaccharides.

关键词： Infant formula Prebiotics ussing chamber Intestinal enzyme activities Phosphate absorption

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Saturation Characteristics of Glucose Transport in Bovine Retinal Pigment Epithelium

引用

眼科学报 1998年第3期14卷 126-129页

作者： Chi Ho To Kin Kwok Cheung Shing Hon Chiu Hon Ming Lai Ka Shun Lung Laboratory of Ocular Physiology and Biochemistry Department of Optometry and Radiography The Hong Kong Polytechnic UniversityHung HomKawloonHong Kong

Purpose:To study the saturation characteristics of the glucose transport across the bovine retinal pigment epithelium(RPE).Methods:The bovine RPE preparations were munted with a modified ussing *** L-[^(3)H]-glucose and 3-0-methyl-D-[^(14)C]-glucose fluxes across the RPE from the choroid to retina were studied at different glucose ***:The glucose transport was found to be stereospecific,with 3-0-methyl-D-glucose(MDG)being transported about three times faster than *** glucose transport showed typical saturation characteristics in Michaelis-Menten *** V_(max)and the K_(m)of corrected MDG were 2452 nmol cm^(-2)h^(-1)and 30.8 mM *** was shown that the glucose transport system was saturated at 61.6 ***:The saturation characteristics of the corrected MDG flux suggested that the capacity of glucose transport through the bovine RPE is immense.

关键词： ussing chamber retinal pigment epithelium glucose transport saturation kinetics

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

迷迭香酸在大鼠离体小肠中的吸收机制研究

引用

中成药 2023年第6期45卷 1749-1754页

作者：张青青张廷恩陈晓晓冯晓姣李紫微皮佳鑫天津中医药大学组分中药国家重点实验室天津301617 天津中医药大学现代中药发现与制剂技术教育部工程中心天津301617

目的考察迷迭香酸在大鼠离体小肠中的吸收机制。方法ussing chamber分析0.5、1、2、4 mg/mL迷迭香酸在肠黏膜中的透过情况,探讨P-糖蛋白抑制剂维拉帕米对该成分肠黏膜透过的影响,以及不同辅料(泊洛沙姆188、聚氧乙烯35蓖麻油、吐温80)... 详细信息

目的考察迷迭香酸在大鼠离体小肠中的吸收机制。方法ussing chamber分析0.5、1、2、4 mg/mL迷迭香酸在肠黏膜中的透过情况,探讨P-糖蛋白抑制剂维拉帕米对该成分肠黏膜透过的影响,以及不同辅料(泊洛沙姆188、聚氧乙烯35蓖麻油、吐温80)对其小肠吸收的促进作用。结果迷迭香酸在大鼠十二指肠、空肠、回肠中的吸收速率与其质量浓度呈线性关系(r>0.90),加入维拉帕米后吸收提高(P<0.05)。3 mg/mL泊洛沙姆188、3 mg/mL聚氧乙烯35蓖麻油增加迷迭香酸在空肠、回肠中的吸收(P<0.05)。结论迷迭香酸在大鼠离体小肠中的吸收机制为被动扩散,其肠黏膜吸收过程受P-糖蛋白转运体影响,泊洛沙姆188、聚氧乙烯35蓖麻油可促进其吸收。

关键词：迷迭香酸吸收机制离体小肠 ussing chamber

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Rectification of oxygen transfer through the rat colonic epithelium

引用

World Journal of Gastrointestinal Pathophysiology 2017年第2期8卷 59-66页

作者： Fernando D Saraví Graciela E Carra Daniel A Matus Jorge E Ibánez Institute of Physiology Faculty of Medical SciencesNational University of Cuyo Department of Surgery Faculty of Medical SciencesNational University of Cuyo Mario H. Burgos Institute of Histology and Embryology CONICETFaculty of Medical SciencesNational University of Cuyo

AIM To assess whether higher sensitivity of colonic epithelium to hypoxia at the serosal side is associated with oxygen transfer *** Rats were fed either with normal chow or a lowsodium diet. Tissues were mounted as flat sheets in a modified, airtight ussing chamber with oxygen meters in each hemichamber. Mucosal samples from normal diet animals were studied under control conditions, in low-chloride solution and after adding chloride secretion inhibitors and chloride secretagogues. Samples from sodium-deprived rats were studied before and after ouabain addition. In separate experiments, the correlation between short-circuit current and oxygen consumption was analyzed. Finally, hypoxia was induced in one hemichamber to assess the relationship between its oxygen content and the oxygen pressure differencebetween both hemichambers. RESULTS In all studied conditions, oxygen consumption was larger in the serosal hemichamber than in the mucosal one(P = 0.0025 to P chamber(r = 0.754; P = 0.011) during mucosal hypoxia, but not with oxygen consumption in the mucosal hemichamber. When hypoxia was induced in the mucosal hemichamber, an oxygen pressure difference of 13 k Pa with the serosal hemichamber was enough to keep its oxygen content constant. However, when hypoxia was induced in the serosal hemichamber, the oxygen pressure difference with the mucosal hemichamber necessary to keep its oxygen content constant was 40 k Pa(P < 0.0001). CONCLUSION Serosal oxygen supply is more readily available to support short-circuit current. This may be partly due to a rectifying behavior of transepithelial oxygen transfer.

关键词： Colonic epithelium Hypoxia Oxygen diffusion Short-circuit current ussing chamber

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

大黄素与P-gp、MRP2、MRP3相互作用及与黑豆主要成分配伍的研究

大黄素与P-gp、MRP2、MRP3相互作用及与黑豆主要成分配伍的研究

引用

作者：谭瑞湘广州中医药大学

学位级别：硕士

目的采用ussing chamber技术建立药物与大鼠肠粘膜MRP2、MRP3的肠道转运实验模型,并应用这两种模型分别研究大黄素与MRP2、MRP3的相互关系。此外,在课题组前期研究基础之上,考察大黄素对P-gp的影响,并将大黄素与黑豆中主要单体成分染料... 详细信息

目的采用ussing chamber技术建立药物与大鼠肠粘膜MRP2、MRP3的肠道转运实验模型,并应用这两种模型分别研究大黄素与MRP2、MRP3的相互关系。此外,在课题组前期研究基础之上,考察大黄素对P-gp的影响,并将大黄素与黑豆中主要单体成分染料木素,染料木苷,大豆苷元,大豆苷进行配伍实验,探讨黑豆主要成分是否影响大黄素在肠道内的吸收转运。方法:1.建立药物与大鼠肠粘膜MRP2、MRP3相互关系的实验验证模型。(1)在大鼠肠组织粘膜侧加入MRP2抑制剂MK-571后,考察MRP2底物普伐他汀经大鼠十二指肠、空肠、回肠段吸收方向与分泌方向的表观渗透系数Papp及外排转运率Er值的变化,若加入抑制剂MK-571后底物普伐他汀经肠转运吸收方向Papp增加或分泌方向Papp减少及外排转运率减少,则表明该实验验证模型建立成功。(2)在大鼠肠组织粘膜侧加入MRP3抑制剂苯溴马隆后,考察MRP3底物替尼泊苷经大鼠空肠、回肠及结肠吸收方向与分泌方向表观渗透系数Papp及外排转运率Er值的变化,若加入抑制剂苯溴马隆后底物替尼泊苷经肠转运吸收方向表观渗透系数增加或分泌方向表观渗透系数及外排转运率减少,表明采用该技术研究药物与肠道MRP3实验验证模型建立成功。2.应用已建立的实验模型,研究大黄素与MRP2之间的相互关系。(1)考察在大鼠肠粘膜组织加入不同浓度大黄素后,MRP2底物普伐他汀在大鼠空肠段吸收方向的表观渗透系数与分泌方向的渗透系数及外排转运率的变化情况,与不含大黄素的对照组相比,研究大黄素对底物普伐他汀经肠吸收的影响,推测大黄素是否为MRP2的抑制剂或诱导剂。(2)在大鼠肠组织粘膜侧加入MRP2抑制剂MK-571后,不同浓度大黄素在大鼠空肠段吸收方向与分泌方向表观渗透系数及外排转运率的变化,将其与不含抑制剂MK-571的对照组相比,据此推测大黄素是否为MRP2的底物。3.应用已建立的实验模型,研究大黄素与MRP3之间的相互关系。(1)考察在大鼠肠粘膜组织加入不同浓度大黄素后,MRP3底物替尼泊苷在大鼠空肠段吸收方向的表观渗透系数与分泌方向的渗透系数及外排转运率的变化情况,与不含大黄素的对照组相比,研究大黄素对底物替尼泊苷经肠吸收的影响,推测大黄素是否为MRP3的抑制剂或诱导剂。(2)在大鼠肠组织粘膜侧加入MRP3抑制剂苯溴马隆后,不同浓度大黄素在大鼠空肠段吸收方向与分泌方向表观渗透系数及外排转运率的变化情况,将其与不含抑制剂苯溴马隆的对照组相比,据此推测大黄素是否为MRP3的底物。4.考察大黄素对P-gp底物地高辛经肠吸收的影响,判断大黄素是否为P-gp的抑制剂或诱导剂。在肠组织粘膜侧加入不同浓度大黄素时,观察底物地高辛在大鼠空肠段吸收方向与分泌方向的表观渗透系数及外排转运率的变化情况。5.在大鼠肠组织粘膜侧分别加入黑豆中四个主要单体成分染料木素、染料木苷、大豆苷元、大豆苷后,考察大黄素在大鼠空肠段吸收方向与分泌方向的表观渗透系数及外排转运率的变化情况,将其与不含黑豆单体成分的对照组相比较,初步探索黑豆中主要单体成分对大黄素经肠吸收转运的影响。结果:1.(1)对照组中普伐他汀经大鼠十二指肠、空肠、回肠分泌方向的表观渗透系数分别为 28.73±16.52、49.60±22.56、67.84±32.52。与对照组相比,浓度为 100μg/mL的MK-571使普伐他汀在各肠段的分泌方向的表观渗透系数降低,其中空肠(12.72 ±3.34)与回肠(22.45 ±14.09)有统计学差异,十二指肠段无统计学差异。100 μ g/mL的MK-571可减少普伐他汀经大鼠十二指肠、空肠及回肠段的外排转运率,且空肠与回肠段具有显著性差异。(2)与对照组相比,200 μg/mL的苯溴马隆可使替尼泊苷经大鼠空肠、回肠、结肠段吸收方向的表观渗透系数增多,其中空肠段(3.72±0.97)有显著差异(P<0.01);200 μ g/mL的苯溴马隆使替尼泊苷经大鼠空肠,回肠及结肠段分泌方向的表观渗透系数降低,与对照组相比,空肠,回肠与结肠段均有显著统计学差异;200 μg/mL的苯溴马隆减少了替尼泊苷经大鼠空肠、回肠及结肠段的外排转运率,且均具有统计学差异(P<0.01)。2.(1)与对照组相比,加入不同浓度大黄素后,低、中、高浓度组中普伐他汀经空肠段吸收方向的表观渗透系数有所增加,但均无统计学意义,分泌方向的表观渗透系数及外排转运率无明显改变。(2)联合使用抑制剂MK-571后,低浓度组,大黄素经空肠段吸收方向表观渗透系数增加,分泌方向表观渗透系数及外排转运率减少,但均无统计学差异;中浓度组,大黄素经空肠段吸收方向表观渗透系数增加(P<0.01),分泌方向表观渗透系数及外排转运率减少,具有统计学意义;高浓度组,大黄素经空肠段吸收方向表观渗透系数增加(P<0.

关键词：大黄素 P-糖蛋白 MRP2 MRP3 ussing chamber 黑豆单体成分

来源：

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

微生态制剂对大肠癌患者术后炎性反应和肠道屏障功能的影响

微生态制剂对大肠癌患者术后炎性反应和肠道屏障功能的影响

引用

作者：杨振上海交通大学

学位级别：硕士

目的:观察微生态制剂对大肠癌患者术后炎性反应及其肠道微生态和屏障功能的影响。方法:采用随机化原则,将入院大肠癌待手术患者共40例按分为实验组(n=20)和对照组(n=20),对照组按常规方式处理,实验组入院后第2天予以口服微生态制剂(优... 详细信息

目的:观察微生态制剂对大肠癌患者术后炎性反应及其肠道微生态和屏障功能的影响。方法:采用随机化原则,将入院大肠癌待手术患者共40例按分为实验组(n=20)和对照组(n=20),对照组按常规方式处理,实验组入院后第2天予以口服微生态制剂(优菌多1包/日),术前平均服用6.5天,手术后待肠功能恢复后继续服用至出院(术后平均服用9天),于手术后观察临床结果,包括术后SIRS发生率、术后感染相关并发症(血液、切口、肺部、静脉导管等)、术后肠功能恢复情况(术后首次排气时间、术后腹泻发生率和腹泻总天数)、术后发热持续时间和住院时间;于手术中切取组织标本常规形态学观察;结肠和末端回肠组织即刻剥离粘膜,置于ussing chamber中,测定两组肠上皮阻抗;以免疫组化方法测定组织标本中跨膜结合蛋白occludin和IgA的含量;手术后第1天抽取外周静脉血,实时定量荧光PCR测定手术后外周血细菌DNA片断阳性率;两组各取术后首次粪便,利用细菌DNA指纹图谱分析技术比较两组手术后粪便菌群变化情况。应用统计软件SPSS10.0 for windows对计量资料进行t检验,计数资料进行x2检验。结果: 1.临床结果:两组术后SIRS发生率、腹腔引流管置放时间、术后感染相关并发症(切口、肺部、静脉导管等)及术后低蛋白血症发生率基本一致,差异无统计学意义(P>0.05);实验组术后首次排气时间(3.3±1.17d)较对照组(4.2±1.32d)为短(Pussing chamber检测上皮阻抗数值较对照组为高,但差异无统计学意义(P>0.05)。 5.粪便细菌DNA指纹图谱分析:细菌DNA指纹图谱显示对照组组图谱条带较实验组明显减少,且出现异常条带。 6.实时定量荧光PCR:对照组术后第1天外周血细菌DNA阳性率(35%)高于实验组(10%)(P<0.05)。结论:肠癌患者使用微生态制剂后,可促进术后肠功能的恢复(术后首次排气时间缩短,腹泻发生率和腹泻总天数下降),缩短术后发热持续时间;增加结肠上皮跨膜结合蛋白occludin和sIgA表达;并可改善肠道菌群多样性,降低术后早期外周血细菌DNA阳性率。

关键词：微生态制剂肠道黏膜屏障紧密连接蛋白 ussing chamber 细菌DNA指纹图谱实时定量荧光PCR

来源：

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

高盐碱环境下青海湖裸鲤饮水率及肠道电生理特征

高盐碱环境下青海湖裸鲤饮水率及肠道电生理特征

引用

作者：朱琳上海海洋大学

学位级别：硕士

青海湖裸鲤(Gymnocypris przewalskii),属鲤形目(Cypriniformes),鲤科(Cyprinidae),裸鲤属(Gymnocypris),是青海湖中唯一的水生经济动物,在整个青海湖生态系统中占据核心地位。青海湖是我国最大的内陆咸水湖泊,由于蒸发量大,盐碱化程度... 详细信息

青海湖裸鲤(Gymnocypris przewalskii),属鲤形目(Cypriniformes),鲤科(Cyprinidae),裸鲤属(Gymnocypris),是青海湖中唯一的水生经济动物,在整个青海湖生态系统中占据核心地位。青海湖是我国最大的内陆咸水湖泊,由于蒸发量大,盐碱化程度高,p H值高达9.1～9.5,含盐量达13～15,且地处青藏高原,水温低,湖面冰封时间长,导致裸鲤生长十分缓慢。课题组近年来对青海湖裸鲤适应高盐碱环境的生理机制研究发现,青海湖裸鲤启动酸碱调节、渗透调节及免疫调节适应盐碱环境,肠道HCO分泌相关基因表达上调,但是对与裸鲤肠道上皮细胞如何进行离子调节规律还有待进一步研究。本论文从盐碱环境下裸鲤肠道上皮离子调节规律出发,从生理水平检测裸鲤饮水率,建立适用于盐碱环境的鱼类肠道ussing chamber电生理平台监测肠道上皮电生理特征,从基因和蛋白水平研究裸鲤渗透及酸碱调节相关基因VHA(V-H-ATPase)、SLC26A6(Cl/HCO交换子)、SLC4A4(Na-HCO联合转运子)的分布和表达情况,以期探究盐碱水中青海湖裸鲤饮水率及电生理特征,进而为裸鲤的耐盐碱机制提供科学依据。1.高盐碱环境下青海湖裸鲤饮水率和肠道胃蛋白酶活力。依据青海湖水近年来的盐碱度以及蒸发量大的特征,设置了盐度为15和17的盐碱水组,并在高原自然环境下取青海湖水做对照试验,结果如下:盐度15的盐碱组(L15)、盐度17的盐碱组(L17)以及高原湖水组(L)在4:00和22:00的饮水率均显著高于10:00和16:00(Pussing chamber电生理平台,设置了盐度为17的海水组(S17)和盐度为17的盐碱水组(L17),结果显示:盐度17的海水组在16:00的短路电流(Isc)最高,10:00的Isc最低,且显著低于其他时间点(P0.05),S17组和L17组在4:00和22:00存在显著性差异(P<0.05)。S17组4:00的跨膜电位(TEP)显著高于16:.00和22:00(P<0.05),S17组和L17组在各个时间点都存在显著性差异(P<0.05),S17组是正向电压,L17组是负向电压。以上结果表明,裸鲤在白天16:00左右穿越肠上皮的离子量总和大于夜间,而碱度是导致裸鲤的TEP改变方向的重要原因。3.为探究裸鲤在盐碱环境下与肠上皮离子调控相关基因的表达,测定了盐碱环境下不同时间点的裸鲤肠道基因相对表达量。结果显示:在S17的胁迫下,裸鲤肠道的VHA、SLC26A6、SLC4A4基因在不同时间点的表达量具有显著性变化(P<0.05)。VHA基因在10:00和16:00的表达量显著高于其他时间点(P<0.05);SLC26A6基因和SLC4A4基因在4:00和22:00的表达量显著高于其他时间点(P<0.05)。在L17的胁迫下,裸鲤肠道的VHA、SLC26A6、SLC4A4基因在不同时间点的表达量具有显著性变化(P<0.05)。VHA基因与SLC4A4基因的表达趋势与在S17胁迫下相一致,SLC26A6基因在22:00的表达量显著高于其他时间点(P<0.05)。以上结果表明,裸鲤VHA基因在白天的相对表达量高于夜间,而SLC26A6与SLC4A4相对表达量与VHA基因相反,即在夜间的表达量高于白天,说明VHA、SLC26A6、SLC4A4基因均参与裸鲤的酸碱及渗透调节,而VHA基因可能还与消化有关系,这有待进一步探究。4.为进一步探究青海湖裸鲤肠道VHA蛋白的分布与表达情况,定

关键词：青海湖裸鲤饮水率 ussing chamber 电生理 V-H+-ATPase蛋白酸碱渗透调节

来源：

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

Regional intestinal transport kinetics of Glycyrrhizin in rats:relative contribution of multiple xenobiotic transporter-mediated efflux and passive diffusion

Regional intestinal transport kinetics of Glycyrrhizin in ra...

引用

第九届全国药物和化学异物代谢学术会议

作者： LI Guo-fu~(1,2),YANG Jin~(1*),CHEN Chun~1,CHEN Rui~3,HUA Xiao-yi~(1,3),ZHAO Hao-ru~(2*) 1 Key Laboratory of Drug Metabolism and Pharmacokinetics,China Pharmaceutical University, Nanjing 210009,Jiangsu,China 2 Department of Pharmaceutics of Traditional Chinese Medicine, China Pharmaceutical University,Nanjing 210009,Jiangsu,China 3 Department of Pharmaceutical analysis,China Pharmaceutical University,Nanjing 210009,Jiangsu,China

The purpose of this work was to investigate the involvement of efflux transporters in the intestinal absorption of GZ,and to split the contribution of the secretion transporters and passive *** transport and inhibition studies of GZ were performed by ussing chamber *** role of paracellular pathway was also *** permeation profile of GZ was confirmed by triple single-pass intestinal perfusion,in the presence *** *** transport of GZ was direction,concentration and temperature dependent for different intestinal *** efflux transport was observed in each segment of intestine,and the contribution of carrier-mediated efflux to the total transport was calculated to be from 70%to 90%at low *** kinetic constant via passive diffusion ranged from 14. 01(2.28 to 37.89(1.06 nl·min·***2 inhibitors mostly abolished the differences in the directional flux for GZ in proximal intestinal regions,in a time-dependent manner,whereas P-gp inhibitors displayed a little effect on the GZ *** played a very limit role to its ***,these results implied that combined impact of MRP2 and P-gp dominates GZ efflux along the whole small intestine,and makes the efflux transport dominate the transepithelial transport process.

关键词： Glycyrrhizin saturated transport mechanism P-glycoprotein multidrug resistance protein 2 ussing chamber triple single-pass intestinal perfusion

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：