The Saturation Characteristics of Glucose Transport in Bovine Retinal Pigment Epithelium

作     者：Chi Ho To Kin Kwok Cheung Shing Hon Chiu Hon Ming Lai Ka Shun Lung CHI HO TO;KIN KWOK CHEUNG;SHING HON CHIU;HON MING LAI;KA SHUN LUNG;LABORATORY OF OCULAR PHYSIOLOGY AND BIOCHEMISTRY;DEPARTMENT OF OPTOMETRY AND RADIOGRAPHY THE HONG KONG POLYTECHNIC UNIVERSITY;HUNG HOM;KAWLOON;HONG KONG

作者机构：Laboratory of Ocular Physiology and BiochemistryDepartment of Optometry and Radiography The Hong Kong Polytechnic UniversityHung HomKawloonHong Kong 

出 版 物：《眼科学报》 (Eye Science)

年 卷 期：1998年第14卷第3期

页      面：126-129页

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：Correspondence address:Chi Ho T0,PhD Assistant Professor,Departnent of Optometry and Radiography.The Hong Kong Poly-lechric University,Hung Hom,Kowloon,Hong Kong.Telephone:(0852)27666102,Fax mumber (0852)27646051,Email:ORCHTO@P0LYU.EDU.HK 

主　　题：ussing chamber retinal pigment epithelium glucose transport saturation kinetics 

摘      要：Purpose:To study the saturation characteristics of the glucose transport across the bovine retinal pigment epithelium(RPE).Methods:The bovine RPE preparations were munted with a modified Ussing *** L-[^(3)H]-glucose and 3-0-methyl-D-[^(14)C]-glucose fluxes across the RPE from the choroid to retina were studied at different glucose ***:The glucose transport was found to be stereospecific,with 3-0-methyl-D-glucose(MDG)being transported about three times faster than *** glucose transport showed typical saturation characteristics in Michaelis-Menten *** V_(max)and the K_(m)of corrected MDG were 2452 nmol cm^(-2)h^(-1)and 30.8 mM *** was shown that the glucose transport system was saturated at 61.6 ***:The saturation characteristics of the corrected MDG flux suggested that the capacity of glucose transport through the bovine RPE is immense.