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Journal of Environmental Sciences 2011年第6期23卷 1038-1043页

作者： Yasushi Hasebe Department of Material Science and Engineering Graduate School of EngineeringSaitama Institute of Technology1690 FusaijiFukayaSaitama 369-0293Japan Department of Life Science and Green Chemistry Saitama Institute of Technology1690 FusaijiFukayaSaitama 369-0293Japan

tyrosinase（TYR） was covalently immobilized onto amino-functionalized carbon felt surface via glutaraldehyde-coupling under ultrasonic treatment for 10 *** resulting TYR-immobilized carbon felt was used as a working electrode unit of bioelectrocatalytic flow-through detector for TYR substrates（catechol,p-chlorophenol（p-CP）,p-cresol,phenol etc.）.Cathodic peak currents based on the electroreduction of enzymatically produced o-quinones were detected at-50 mV ***/*** with previous work in which TYR was immobilized onto amino-functionalized carbon felt for 16 hr without the ultrasonic treatment,we succeeded in（1） shortening the enzyme immobilization time from 16 hr to 10 min,（2） enhancing the sensitivity of p-CP,and（3） improving the operational stability of *** ultrasonic treatment during the TYR immobilization step would lead to certain changes in the structure of the immobilized TYR and the morphology of the immobilized TYR-layer on the carbon felt surface.

关键词： enzyme immobilization under ultrasonic treatment flow-biosensor carbon felt tyrosinase phenolic compounds

tyrosinase,a new innate humoral immune parameter in large yellow croaker(Pseudosciaena crocea R)

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Chinese Journal of Oceanology and Limnology 2009年第3期27卷 527-535页

作者：王淑红王艺磊张子平谢芳靖林鹏邰振刚 The Key Laboratory of Science and Technology for Aquaculture and Food Safety Fisheries CollegeJimei University Department of Chemistry and Biochemistry Texas State University

We evaluated the immune response to infection with a pathogen in large yellow croaker(Pseudosciaena crocea Richardson).The fish were given an intraperitoneal(i.p.) injection of Vibrio parahaemolyticus or sterile sea water(control).We collected blood sera from the fish 0.17,1,2,4,8,12,or 16 d after injection(dpi).We measured tyrosinase activity and the concentrations of lysozyme,NOS,and *** tyrosinase activity was significantly higher at 0.17 and 4 dpi than in the control group,and peaked at 8 *** activity was significantly higher at 2 and 12 dpi than in the control group,but lower at 16 *** is no statistical difference in the level of nitric oxides synthase(NOS) activity or antibodies between the control and injection *** is the first report of the tyrosinase activity in the serum of large yellow *** results indicate that tyrosinase plays an important role in the immediate immune defense against *** in large yellow *** is a candidate parameter for investigation of fish innate immune defense.

关键词： pseudosciaena crocea tyrosinase lysozyme nitric oxide synthase (NOS) natural antibodies

Catalysis-based specific detection and inhibition of tyrosinase and their application

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Journal of Pharmaceutical Analysis 2020年第5期10卷 414-425页

作者： Yunwei Qu Qing Zhan Shubo Du Yang Ding Bin Fang Wei Du Qiong Wu Haidong Yu Lin Li Wei Huang Key Laboratory of Flexible Electronics(KLOFE)&Institute of Advanced Materials(IAM) Nanjing Tech University(NanjingTech)NanjingPR China Department of Chemistry National University of SingaporeSingapore Frontiers Science Center for Flexible Electronics Xi’an Institute of Flexible Electronics(IFE)and Xi’an Institute of Biomedical Materials&EngineeringNorthwestern Polytechnical University127 West Youyi RoadXi’an710072China

tyrosinase is an important enzyme in controlling the formation of melanin in melanosome,and plays a key role in the pigmentation of hair and *** abnormal expression or activation of tyrosinase is associated with several diseases such as albinism,vitiligo,melanoma and Parkinson *** deposition of melanin could cause diseases such as freckles and brown spots in the human body,and it is also closely related to browning of fruits and vegetables and insect *** and inhibiting the activity of tyrosinase is of extraordinary value in the progress of diagnosis and treatment of these ***,many selective optical detection probes and small molecular inhibitors have been developed,and have made significant contributions to the basic and clinical research on these *** this paper,the detection and inhibition of tyrosinase and their application in whitening products are reviewed,with special emphasis on development of fluorescent probes and ***,this review will help design more efficient and sensitive tyrosinase probes and inhibitors,as well as shed light on novel treatment of diseases such as melanoma.

关键词： tyrosinase Melanin Detection probe Inhibitors Melanoma

CuO/Cu2O nanowire array photoelectrochemical biosensor for ultrasensitive detection of tyrosinase

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Science China Chemistry 2020年第7期63卷 1012-1018页

作者： Xiaoxi Guo Jinghua Wu Lian Xia Meihao Xiang Fengli Qu Jinghong Li College of Chemistry and Chemical Engineering Qufu Normal UniversityQufu 273165China Department of Chemistry Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical BiologyTsinghua UniversityBeijing 100084China

Photoelectrochemical(PEC) biosensors have shown great promise in bioanalysis and diagnostic applications in recent years. In this work, the CuO/Cu2O nanowire array(CuO/Cu2O Nanowire) supported on copper foam was prepared as a photocathode for detection of tyrosinase though quinone-chitosan conjugation chemistry method. The in-situ generated quinones that were the catalytic product of tyrosinase acted as electron acceptors, which were captured by the chitosan deposited on the surface of the electrode. Direct immobilization of electron acceptor on the electrode surface improved the photocurrent conversion efficiency and thus sensitivity. The as-prepared biosensor can realize a rapid response in a wide linear range of 0.05 U/mL to 10 U/mL with the detection limit as low as 0.016 U/mL of tyrosinase. The current work provides a new perspective to design and develop highly sensitive and selective PEC biosensor.

关键词： tyrosinase Cu0/Cu20 nanowire photoelectrochemical biosensor chitosan modified electrode quinones

Evaluation of a resoruf in-based fluorescent probe for tyrosinase detection in skin pigmentation disorders

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Bio-Design and Manufacturing 2021年第4期4卷 806-817页

作者： Yibo Hu Hongliang Zeng Jiaxing Jiang Sheng Yang Jinhua Huang Jing Chen Qinghai Zeng Department of Dermatology The Third Xiangya Hospital of Central South UniversityTongzipo Road No.138Yuelu DistrictChangsha 410013China Institute of Chinese Materia Medica Hunan Academy of Chinese MedicineChangsha 410006China School of Chemistry and Food Engineering Changsha University of Science and Technology(CSUST)Changsha 410114China

Purpose Skin pigmentation disorders,such as vitiligo and melasma,are difficult to diagnose in the early stages,but abnor-mal tyrosinase levels and tyrosinase activity are potential *** resorufin-based fluorescence probes(RBFPs)have been designed to detect tyrosinase in tumors,but they have not been used in skin pigmentation *** this study,one of these RBFPs(synthesized by resorufin salt coupled with 3-(bromomethyl)phenol)was evaluated *** The RBFP was tested in different kinds of mouse and human skin cells,as well as in in vivo models,including zebrafish,guinea pigs,and Sprague-Dawley *** addition,small interfering RNAs(siRNAs),kojic acid,and 1-phenyl-2-thiourea(PTU)were used to inhibit tyrosinase levels or tyrosinase *** This probe successfully detected tyrosinase and emitted red fluorescence in melanoma cells and *** was also observable in zebrafish and on the skin of guinea pigs when using the *** mouse and human cells,the RBFP showed good selectivity to ***,in the case of decreased tyrosinase levels or activity caused by siRNAs,kojic acid,or PTU,the probe was sensitive to these ***,the RBFP showed no toxic effects at concentrations oftyrosinase detection,but suggest the need for further improvement of fluorescent probes in the diagnosis of skin pigmentation disorders.

关键词： tyrosinase Pigmentation disorder Diagnosis Fluorescence probe

Design, Synthesis and Biological Activity of Novel Furocoumarin Derivatives as Stimulators of Melanogenesis and tyrosinase in B16 Cells

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Chemical Research in Chinese Universities 2018年第3期34卷 408-414页

作者： NIU Chao ZANG Deng Haji Akber AISA Key Laboratory of Plant Resources and Chemistry in Arid Regions State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization Xinjiang Technical Institute of Physics and Chemistry Chinese Academy of Sciences Urumqi 830011 P. R. China University of Chinese Academy of Sciences Beijing 100049 P. R. China

Sixteen novel and four known（4a, 4d, 4e, 4h） amine derivatives of furocoumarin were synthesized, then submitted to evaluation as stimulators of melanogenesis and tyrosinase in B16 murine cells. Among them, five compounds（4g, 4j-4m） showed potent activating effect on both melanogenesis and tyrosinase in vitro compared with positive control（8-MOP）, the most widely used drugs for vitiligo in clinic. Noticeably, compotmds 4h and 4j, which contain morpholine and piperazine, were recognized as the most effective stimnlator of melanogenesis and tyrosianse in B16 cells separately. These derivatives may serve as lead compounds for further drug discovery for the treatment of vitiligo.

关键词： Furocoumarin Amine Melanin synthesis tyrosinase Vitiligo

Isolation and characterization of tyrosinase produced by marine actinobacteria and its application in the removal of phenol from aqueous environment

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Frontiers in Biology 2014年第4期9卷 306-316页

作者： Suki ROY Ishita DAS Minki MUNJAL Loganathan KARTHIK Gaurav KUMAR Sathish KUMAR Kokati Venkata Bhaskara RAO Molecular and Microbiology Research Laboratory School of Bio Sciences and Technology VIT University Vellore-632014 Tamil Nadu India Department of Biotechnology Shri JJT University Jhunjhunu Raiasthan India

The present study was focused on screening and characterization of tyrosinase enzyme produced by marine actinobacteria and its application in phenolic compounds removal from aqueous solution. A total of 20 strains were isolated from marine sediment sample and screened for tyrosinase production by using skimmed milk agar medium. Among 20 isolates, two isolates LK-4 and LK-20 showed zone of hydrolysis and these were taken for secondary screening by using tyrosiue agar medium. Based on the result of secondary screening LK-4 was selected for further analysis, such as tyrosinase assay, protein content and specific activity of the enzyme. The tyrosinase enzyme was produced in a SS medium and was partially purified by ammonium sulfate precipitation, dialysis and SDS PAGE. The isolate （LK-4） was identified as Streptomyces espinosus using 16S rRNA gene sequencing and named as ＂Streptomyces espinosus strain LK4 （KF806735）＂. The tyrosinase enzyme was immobilized in sodium alginate which was applied to remove phenolic compounds from water. The enzyme efficiently removed the phenolic compounds from aqueous solution within few hours which indicated that tyrosinasc enzyme produced by Streptomyces espinosus strain LK-4 can be potently used for the removal of phenol and phenolic compounds from wastewater in industries.

关键词： actinobacteria phenolic compounds immobilization tyrosinase

Epimedin B exhibits pigmentation by increasing tyrosinase family proteins expression,activity,and stability

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Journal of Pharmaceutical Analysis 2024年第1期14卷 69-85页

作者： Chen Hong Yifan Zhang Lili Yang Haoyang Xu Kang Cheng Zhi Lv Kaixian Chen Yiming Li Huali Wu Department of TCM Chemistry School of PharmacyShanghai University of Traditional Chinese MedicineShanghai200000China Department of Dermatology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai200000China International Education College Shanghai University of Traditional Chinese MedicineShanghai200000China Shanghai Inoherb Cosmetics Co. Ltd.Shanghai200000China

Epimedin B(EB)is one of the main flavonoid ingredients present in Epimedium brevicornum Maxim.,a traditional herb widely used in *** previous study showed that EB was a stronger inducer of melanogenesis and an activator of tyrosinase(TYR).However,the role of EB in melanogenesis and the mechanism underlying the regulation remain ***,as an extension to our previous investigation,we provide comprehensive evidence of EB-induced pigmentation in vivo and in vitro and elucidate the melanogenesis mechanism by assessing its effects on the TYR family of proteins(TYRs)in terms of expression,activity,and *** results showed that EB increased TYRs expression through microphthalmia-associated transcription factor-mediated p-Akt(referred to as protein kinase B(PKB))/glycogen synthase kinase 3β(GSK3β)/β-catenin,p-p70 S6 kinase cascades,and protein 38(p38)/mitogen-activated protein(MAP)kinase(MAPK)and extracellular regulated protein kinases(ERK)/MAPK pathways,after which EB increased the number of melanosomes and promoted their maturation for melanogenesis in melanoma cells and human primary melanocytes/skin ***,EB exerted repigmentation by stimulating TYR activity in hydroquinone-and N-phenylthiourea-induced TYR inhibitive models,including melanoma cells,zebrafish,and ***,EB ameliorated monobenzone-induced depigmentation in vitro and in vivo through the enhancement of TYRs stability by inhibiting TYR misfolding,TYR-related protein 1 formation,and retention in the endoplasmic reticulum and then by downregulating the ubiquitination and proteolysis *** data conclude that EB can target TYRs and alter their expression,activity,and stability,thus stimulating their pigmentation function,which might provide a novel rational strategy for hypopigmentation treatment in the pharmaceutical and cosmetic industries.

关键词： Epimedin B Melanosome tyrosinase Melanogenesis

Comparison of structural and physicochemical properties of potato protein and potato flour modified with tyrosinase

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Journal of Integrative Agriculture 2022年第5期21卷 1513-1524页

作者： ZHU Yu YUAN Yu-han MEI Li-ping DING Shuang-kun GAO Yu-chen DU Xian-feng GUO Li Anhui Engineering Laboratory of Agro-products Processing Anhui Agricultural UniversityHefei 230036P.R.China State Key Laboratory of Biobased Material and Green Papermaking School of Food Sciences and EngineeringQilu University of Technology(Shandong Academy of Sciences)Jinan 250353P.R.China College of Life Science and Technology Xinjiang UniversityUrumchi 830046P.R.China

The present study modified potato protein and flour with tyrosinase to promote the diversification of potato staple *** results indicated that tyrosinase treatment markedly altered the secondary structure of *** tyrosinase treatment,the maximum decomposition temperature of potato protein and flour increased from 322.32 to 332.40℃ and from 294.24 to 299.61℃,*** treatment remarkably reduced the pasting viscosity of potato flour,that is,the peak viscosity,through reducing viscosity,breakdown,final viscosity,and setback by 32.50,60.98,13.04,68.24,and 74.31%,*** contrast,tyrosinase treatment increased the shear resistance and hardness of the protein and flour gels;the maximum stress values of the protein and flour gels increased from 1.48 to 10.1% and from 6.87 to 14.8%,***,tyrosinase treatment promoted viscoelastic properties and structural stability of potato protein and *** results may provide an important foundation for the development of novel potato staple foods.

关键词： potato protein starch structural tyrosinase physicochemical features

Targeted disruption of tyrosinase causes melanin reduction in Carassius auratus cuvieri and its hybrid progeny

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Science China(Life Sciences) 2019年第9期62卷 1194-1202页

作者： Qingfeng Liu Yanhua Qi Qiuli Liang Jia Song Junmei Liu Wuhui Li Yuqin Shu Min Tao Chun Zhang Qinbo Qin Jing Wang Shaojun Liu State Key Laboratory of Developmental Biology of Freshwater Fish Hunan Normal UniversityChangsha 410081China College of Life Sciences Hunan Normal UniversityChangsha 410081China

The white crucian carp(Carassius auratus cuvieri,WCC) not only is one of the most economically important fish in Asia,characterized by strong reproductive ability and rapid growth rates,but also represents a good germplasm to produce hybrid progenies with *** knockout technique provides a safe and acceptant way for fish *** gene knockout in WCC and its hybrid progeny will be of great importance for both genetic studies and hybridization ***(TYR) is a key enzyme in melanin *** of tyr in zebrafish and mice results in mosaic pigmentation or total ***,we successfully used CRISPR-Cas9 to target tyr in WCC and its hybrid progeny(WR) derived from the cross of WCC(♀) and red crucian carp(Carassius auratus red var.,RCC,♂).The level of TYR protein was significantly reduced in mutant *** the mutant WCC and the mutant WR showed different degrees of melanin reduction compared with the wild-type sibling control fish,resulting from different mutation efficiency ranging from 60% to 90%.In addition,the transcriptional expression profiles of a series of pivotal pigment synthesis genes,***1,mitfa,mitfb,dct and sox10,were down-regulated in tyr-CRISPR WCC,which ultimately caused a reduction in melanin *** results demonstrated that tyr plays a key role in melanin synthesis in WCC and WR,and CRISPR-Cas9 is an effective tool for modifying the genome of economical ***,the tyr-CRISPR models could be valuable in understanding fundamental mechanisms of pigment formation in non-model fish.

关键词： white crucian carp hybridization CRISPR-Cas9 tyrosinase pigmentation