检索结果-南通市图书馆

Comparative studies on histological and ultra-structure of the pituitary of different ploidy level fishes

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2006年第5期49卷 446-453页

作者： LONG Yu LIU Shaojun HUANG Weiren ZHANG Jian SUN Yuandong ZHANG Chun CHEN Song LIU Jinghui LIU Yun College of Life Sciences Hunan Normal UniversityChangsha 410081China

The histological and ultra-structure of the pituitary in diploid red crucian carp(Carassius auratus red var.),triploid crucian carp and allotetraploid hybrids within and after the breeding season were comparatively *** result showed that there were six endocrine cell types in the pituitary of these three kinds of fishes,and there was an obvious difference in cell size among different ploidy level *** for the same type of pituitary cells,the cell size was increased gradually with the in-creasing ploidy *** the breeding season,the allotetraploid hybrids had higher proportion of go-nadotropin cells(GTH)than triploids,and the triploids had higher proportion of GTH than *** results were related to the earlier sexual maturity of allotetraploid hybrids and sterility of triploid cru-cian *** the other hand,among the three kinds of fishes,the proportion of somatotropin(STH)cells in triploids crucian carp was the highest,whereas that in allotetraploid hybrids was the *** results might be connected with the faster growth rate of triploids and slower growth rate of al-lotetraploid *** addition,in GTH cells of meso-adenohypophysis after the breeding season,there were many endocrine particles in triploids,while those endocrine particles were released from the cells in allotetraploids and *** result showed that the sterility of triploid crucian carp might be related to the hormone which was not released from the GTH *** a word,the present study indicated that the differences in the structure of pituitary among different ploidy level fishes contributed to their difference in the growth rate and gonadal development.

关键词： diploid triploid ailotetraploid pituitary histological structure ultra-structure

Distant hybridization leads to different ploidy fishes

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2010年第4期53卷 416-425页

作者： LIU ShaoJun Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China 1. Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China College of Life Sciences Hunan Normal University Changsha 410081 China

Distant hybridization makes it possible to transfer the genome of one species to another, which results in changes in phenotypes and genotypes of the progenies. This study shows that distant hybridization or the combination of this method with gynogenesis or androgenesis lead to different ploidy fishes with genetic variation, including fertile tetraploid hybrids, sterile triploid hybrids, fertile diploid hybrids, fertile diploid gynogenetic fish, and their derived progenies. The formations of the different ploidy fishes depend on the genetic relationship between the parents. In this study, several types of distant hybridization, including red crucian carp (Carassius auratus red var.) (2n=100, abbreviated as RCC) (♀)×common carp (Cyprinus carpio L.) (2n=100, abbreviated as CC) (♂), and RCC (2n=100) (♀)×blunt snout bream (Megalobrama amblycephala) (2n=48, abbreviated as BSB) (♂) are described. In the distant hybridization of RCC (♀)×CC (♂), bisexual fertile F3–F18 allotetraploid hybrids (4n=200, abbreviated as 4nAT) were formed. The diploid hybrid eggs and diploid sperm generated by the females and males of 4nAT developed into diploid gynogenetic hybrids and diploid androgenetic hybrids, respectively, by gynogenesis and androgenesis, without treatment for doubling the chromosome. Improved tetraploid hybrids and improved diploid fishes with genetic variation were derived from the gynogenetic hybrid line. The improved diploid fishes included the high-body RCC and high-body goldfish. The formation of the tetraploid hybrids was related to the occurrence of unreduced gametes generated from the diploid hybrids, which involved in premeiotic endoreduplication, endomitosis, or fusion of germ cells. The sterile triploid hybrids (3n=150) were produced on a large scale by crossing the males of tetraploid hybrids with females of diploid fish (2n=100). In another distant hybridization of RCC (♀)×BSB (♂), different ploidy fishes were obtained, including diploid bisexual fertile natu

关键词： distant hybridization genetic variation gynogenesis androgenesis diploid triploid tetraploid evolutionary biology genetic breeding

Polyploidy induction by hydrostatic pressure shock and embryo development of sea cucumber Apostichopus japonicus

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Oceanology and Limnology 2007年第2期25卷 184-190页

作者：丁君常亚青王子臣宋坚 School of Environmental & Biological Science & Technology Dalian University Technology Key Laboratory of Mariculture and Biotechnology Ministry of Agriculture Dalian Fisheries University

The manipulation of the chromosome set for commercially valuable marine animals is important for enhancing aquacultural production. In this study, triploid and tetraploid sea cucumber Apostichopus japonicus were induced by hydrostatic pressure shock, and the conditions of appropriate induction were tested with different starting times, and hydrostatic pressure intensities and durations. The highest rate of triploid induction reached 20% and that of tetraploid was 60%. In consideration of the survival rate and hatch rate, the appropriate treatment for triploid was 55 Mpa of hydrostatic pressure for 5 rain at 55 min after fertilization （a.f.）, while for tetraploid it was 60 Mpa for 5 rain at 61 min a.f. The triploid of the sea cucumber could survive through the pelagic larval stage and attachment stage, and develop like the control group of the experiment. The tetraploid, however, could not survive the attachment stage.

关键词： triploid tetraploid hydrostatic pressure shock sea cucumber, Apostichopusjaponicus

细胞松弛素B和低温诱导华贵栉孔扇贝三倍体的产生(英文)

在线全文

学校读者我要写书评

暂无评论

中山大学学报论丛 1995年第3期15卷 47-50页

作者：林岳光庆宁何毛贤姜卫国中国科学院南海海洋研究所广州510301

分别用细胞松驰素B（C．B）（浓度为0．5mg／L）和低温（10℃）处理诱导华贵栉孔扇贝Chlamysnobilis产生三倍体．抑制第一极体的三倍体诱导率达到44．1％和31．8％；而抑制第二极体的三倍体诱导率分别为90．2％和72．7％（胚胎初期检查... 详细信息

分别用细胞松驰素B（C．B）（浓度为0．5mg／L）和低温（10℃）处理诱导华贵栉孔扇贝Chlamysnobilis产生三倍体．抑制第一极体的三倍体诱导率达到44．1％和31．8％；而抑制第二极体的三倍体诱导率分别为90．2％和72．7％（胚胎初期检查）．随着C．B浓度的增加，孵化率下降；而随着温度的降低和休克时间的延长，非整倍体增多．

关键词： Chlamys nobilis cytochalasin B low temerature triploid

Microsatellite Analysis of Genetic Diversity Between Loach with Different Levels of Ploidy

在线全文

学校读者我要写书评

暂无评论

Journal of Northeast Agricultural University(English Edition) 2014年第4期21卷 47-53页

作者： Li Ya-juan Qi Hong-rui Ma Hai-yan Zhou He Xu Wen Sui Yi Li Jia-qi Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province Key Laboratory of Mariculture & Stock Enhancement in North China's Sea Ministry of Agriculture Dalian Ocean University

We used microsatellite markers to investigate the genetic parameters of three different polyploidy populations of Misgurnus anguillicaudatus from Honghu City, Hubei Province. Polyacrylamide gel electrophoresis banding patterns of diploid（2n）, triploid（3n） and tetraploid loaches（4n） were analyzed with Pop Gen software. A total of 68 alleles were obtained from seven microsatellite loci and the polymorphism information content（PIC） indices were all above 0.5. The average expected mean heterozygosity values（He） were 0.8420, 0.7186 and 0.8521; the average observed mean heterozygosity values（Ho） were 0.9674, 0.9785 and 0.8928; and the HardyWeinberg P values were 0.3078, 0.3151 and 0.3762, for diploid, triploid and tetraploid individuals, respectively. The results indicated that the three populations were highly polymorphic, with no deviations from Hardy-Weinberg equilibrium observed at all the seven microsatellite loci. This indicated a high level of genetic diversity within the populations. A cluster analysis diagram showed that the shortest genetic distance was between diploid and tetraploid loaches and they shared a close phylogenetic relationship. The triploid and tetraploid individuals had the most distant phylogenetic relationship.

关键词： Misgurnus anguillicaudatus diploid triploid tetraploid microsatellite genetic diversity

Incidence and molecular markers of 2n pollen in Populus tomentosa Carr

在线全文

学校读者我要写书评

暂无评论

北京林业大学学报 2006年第S2期28卷 176-176页

作者： ZHANG Zheng-hai KANG Xiang-yang ZHANG Ping-dong LI Yan-hua WANG Jun(Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University)

Microscopic examination and AFLP molecular markers were employed to determine the incidence and related markers of 2n pollen (unreduced pollen) in Populus tomentosa Carr. The parallel and tripolar spindle at metaphase Ⅱ and the absence of cytokinesis at telophase Ⅱ led to the formation of 2n pollen. A group of 298 clones came from their indigenous areas was investigated for the production of 2n pollen based on the pollen size differences, within a clone and between n and 2n pollen. Pollen grains of 224 clones were collected, six of them only produced normal pollen, and the rest produced 2n pollen at different frequencies (0.6% -21.9%). Clones producing six normal and 22 2n pollen were selected for AFLP analysis. Following an initial screening with 55 primer combinations, the E50-M38 (CAT/ACT) primer was identified, which generated a PCR fragment (246 bp) from the normal clones, but not from the 2n pollen producers. In addition, the E31-M50 (AAA/CAT) amplified DNA fragment (204 bp) that was present in 2n pollen producers, and absent in normal clones. Two polymorphic bands were found and they were distinguished between normal and 2n pollen clones. They are very useful as AFLP markers for molecular-assisted selection in triploid breeding of 2n gametes in ***.

关键词： AFLP Populus tomentosa Carr. meiosis 2n pollen incidence triploid breeding

在线全文

学校读者我要写书评

暂无评论

鲤鱼染色体组人工调控的核型证明

水生生物学报 1986年第3期10卷 265-270+301页

作者：吴清江,叶玉珍,陈荣德中国科学院水生生物研究所中国科学院水生生物研究所中国科学院水生生物研究所武汉武汉武汉

以囊胚细胞制备鲤鱼单倍体染色体永久片并分析了单倍体染色体的核型。用囊胚细胞及白细胞培养法制取雌核二倍体及人工三倍体的永久片。以单倍体的核型为根据对人工二倍体及三倍体的染色体配对证明,人工诱发雌核二倍体的染色体是来自两... 详细信息

以囊胚细胞制备鲤鱼单倍体染色体永久片并分析了单倍体染色体的核型。用囊胚细胞及白细胞培养法制取雌核二倍体及人工三倍体的永久片。以单倍体的核型为根据对人工二倍体及三倍体的染色体配对证明,人工诱发雌核二倍体的染色体是来自两个完整的染色体组,而人工三倍体的染色体则依不同个体而有差异。有部分个体的染色体来自三套完整的染色体组,但大部分个体是非整倍体,前者的雌性个体性腺发育正常,后者是不育的。

关键词： Karyotype Genome Manipulation Haploid Gynogenetic triploid

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

高产优质西瓜新品种郑抗无籽8号的选育

高产优质西瓜新品种郑抗无籽8号的选育

纪念全国西瓜甜瓜科研与生产协作50周年暨第12次全国西瓜甜瓜学术研讨会

作者：刘文革阎志红何楠赵胜杰中国农业科学院郑州果树研究所

郑抗无籽8号是以MMD为母本,将军为父本杂交育成的新组合。该品种中晚熟,果实发育期37-40天。生长势强,抗病耐湿性好,果实大、产量高,平均单果重8-10 kg,普通栽培亩产4500 kg左右,温室嫁接栽培可达5000 kg以上。果实圆球型,纯黑皮,幼果... 详细信息

郑抗无籽8号是以MMD为母本,将军为父本杂交育成的新组合。该品种中晚熟,果实发育期37-40天。生长势强,抗病耐湿性好,果实大、产量高,平均单果重8-10 kg,普通栽培亩产4500 kg左右,温室嫁接栽培可达5000 kg以上。果实圆球型,纯黑皮,幼果转黑快,

关键词： seedless watermelon triploid New variety Zhengkang Wuzi No.5 High yield

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

不同倍性西瓜果实Vc含量比较研究

不同倍性西瓜果实Vc含量比较研究