Polyploidy induction by hydrostatic pressure shock and embryo development of sea cucumber Apostichopus japonicus
Polyploidy induction by hydrostatic pressure shock and embryo development of sea cucumber Apostichopus japonicus作者机构：School of Environmental & Biological Science & Technology Dalian University Technology Key Laboratory of Mariculture and Biotechnology Ministry of Agriculture Dalian Fisheries University
出 版 物：《Chinese Journal of Oceanology and Limnology》 (中国海洋湖沼学报（英文版）)
年 卷 期：2007年第25卷第2期
页 面：184-190页
核心收录：
基 金：the National High-Tech Research and Development Program of China (863 Program, No.2506AA10A411) by the Dalian Natural Science Foundation (No. 99058)
主 题：triploid tetraploid hydrostatic pressure shock sea cucumber, Apostichopusjaponicus
摘 要：The manipulation of the chromosome set for commercially valuable marine animals is important for enhancing aquacultural production. In this study, triploid and tetraploid sea cucumber Apostichopus japonicus were induced by hydrostatic pressure shock, and the conditions of appropriate induction were tested with different starting times, and hydrostatic pressure intensities and durations. The highest rate of triploid induction reached 20% and that of tetraploid was 60%. In consideration of the survival rate and hatch rate, the appropriate treatment for triploid was 55 Mpa of hydrostatic pressure for 5 rain at 55 min after fertilization （a.f.）, while for tetraploid it was 60 Mpa for 5 rain at 61 min a.f. The triploid of the sea cucumber could survive through the pelagic larval stage and attachment stage, and develop like the control group of the experiment. The tetraploid, however, could not survive the attachment stage.
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