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Antibacterial activity of leaves extracts of trifolium alexandrinum Linn,against pathogenic bacteria causing tropical diseases

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Asian Pacific Journal of Tropical Biomedicine 2012年第3期2卷 189-194页

作者： Abdul Viqar Khan Qamar Uddin Ahmed Indu Shukla Athar Ali Khan Department of Botany Faculty of Life SciencesAligarh Muslim University Department of Pharmaceutical Chemistry Faculty of PharmacyInternational Islamic University Malaysia Department of Microbiology Jawaharlal Nehru Medical CollegeAligarh Muslim University

Objective:To investigate antibacterial potential of trifolium alexandrinum(***) Linn,against seven gram positive and eleven gram negative hospital isolated human pathogenic bacterial strains responsible for many tropical ***:Non-polar and polar extracts of the leaves of *** i.e.,hexane,dichloromethane(DCM),ethyl acetate(EtOAc), methanol(MeOH) and aqueous(AQ) extracts at five different concentrations(1,2,5,10 and 15 mg/ mL) were prepared to evaluate their antibacterial *** standards were strictly followed to perform antimicrobial disc susceptibility test using disc diffusion ***:Polar extracts demonstrated significant antibacterial activity against tested *** and MeOH extracts showed maximum antibacterial activity with higher inhibition zone and were found effective against seventeen of the tested *** AQ plant extract inhibited the growth of sixteen of the test *** and MeOH plant extracts inhibited the growth of all seven gram positive and ten of the gram negative bacterial ***:The present study strongly confirms the effectiveness of crude leaves extracts against tested human pathogenic bacterial strains causing several tropical *** Egyptian clover is used as a fodder plant,it could be helpful in controlling various infectious diseases associated with cattle as well.

关键词： trifolium alexandrinum L. Fabaceae Antibacterial activity Pathogenic bacteria Gram-positive bacteria Gram-negative bacteria Tropical disease Infectious disease

Phenotypic and Genetic Characterization of trifolium Rhizobia Isolated from Temperate and Subtropical Regions of China

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Journal of Northeast Agricultural University(English Edition) 2017年第3期24卷 37-50页

作者： Wu Xing-yue Gao Jun Wang Le Wang Bei-nan Yao Hong-li Liu Zhen-yu Yang Hai-peng Liu Zhong-kuan Liang Zi-teng Wang Fang Liu Xiao-yun Key Laboratory of Microbial Diversity Research and Application of Hebei Province College of Life Sciences Hebei University Institute of Agro-resources and Environment Hebei Academy of Agriculture and Forestry Sciences Key Laboratory of State Forestry Administration for Biodiversity Conservation in Southwest China Southwest Forestry University

The clovers that comprise trifolium genus are naturalized in the subtropical and tropical zones in China. They are valuable bioresources as important green manures and pasture grass, which contribute biologically fixed dinitrogen （N2） and provide nutrition to farming systems. However, there are very few effective strains available for inoculant production and there is little information available about symbiotic rhizobia in Chinese legume clover root nodules. In this study, 139 root nodule bacteria were isolated from two clover species （trifolium repens and trifolium pretense） growing in the subtropical and temperate regions of China, 16S rRNA gene sequence analysis, BOX-PCR, whole cell protein SDS-PAGE, and nodulation tests were performed to characterize these strains. The results showed that phenotypic and genetic diversities among 139 isolates were large, with 83 protein patterns and 66 BOX- AIR profiles, respectively. The rhizobial strains were first divided into two large phenotypic protein groups. The sequencing strains representing the two groups were related to Rhizobium leguminosarum USDA2370T and R. sophorae CCBAU03386T and had 99.6%-100% similarities. The phylogeny specificity of the rhizobia from clover was elucidated, while showed a large variation in the fingerprints of the phenotypes and genotypes and genetic diversity was high （revealed by Shannon diversity index, H＇）. The rhizobial isolates from subtropical regions, such as Anhui Province, Yunnan Province and Hubei Province, had higher diversities than those from temperate areas, such as Hebei Province and Shanxi Province, which could be used to identify rhizobial strains from clover and screen efficient inoculum strains. A number of diverse rhizobial strains had been identified and a pool of currently available clover rhizobial strains were increased. This would ultimately increase the likelihood of identifying more efficient strains suited for developing a successful inoculation strategy for the p

关键词： diversity genotype phenotype rhizobia trifolium

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白三叶根系分泌物对5种草坪草的生化效应

草地学报 2014年第1期22卷 158-165页

作者：余婷温艳斌孟焕文刘同金赵慧玲程智慧西北农林科技大学林学院陕西杨凌712100 西北农林科技大学园艺学院陕西杨凌712100

为探讨根系分泌物在白三叶(trifolium repens L.)入侵禾本科草坪过程中的生化效应,采用水培法收集其根系分泌物,检测不同浓度白三叶根系分泌物对多年生黑麦草(Lolium perenne L.)、紫羊茅(FestucarubraL.)、高羊茅(Festuca arundinacea ... 详细信息

为探讨根系分泌物在白三叶(trifolium repens L.)入侵禾本科草坪过程中的生化效应,采用水培法收集其根系分泌物,检测不同浓度白三叶根系分泌物对多年生黑麦草(Lolium perenne L.)、紫羊茅(FestucarubraL.)、高羊茅(Festuca arundinacea Schreb.)、草地早熟禾(Poapratensis L.)和匍匐翦股颖(Agrostis stolonifera L.)叶片超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)的活性和丙二醛(MDA)含量以及根系活力的影响。结果表明:白三叶根系分泌物对5种草坪草上述生理生化指标均具有浓度效应。在低浓度下(0.002 g·mL^(-1))对SOD和CAT活性具有促进效应,在高浓度下(0.05 g·mL^(-1))则具有抑制效应。5种受体的POD活性和MDA含量均随白三叶根系分泌物浓度增大而升高,根系活力则随着其浓度增大而降低。因此,白三叶根系分泌物可引起受体植物对逆境的响应,5种草坪草化感效应的生化机制可能是分泌物中化感物质通过改变受体幼苗体内保护酶活性及脂膜过氧化来表现的。

关键词：白三叶根系分泌物草坪草化感作用生化指标 trifolium repens L

Root Exudation of Organic Acids of Herbaceous Pioneer Plants and Their Growth in Sterile and Non-Sterile Nutrient-Poor,Sandy Soils from Post-Mining Sites

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Pedosphere 2019年第1期29卷 34-44页

作者： Katja BOLDT-BURISCH Bernd Uwe SCHNEIDER M.Anne NAETH Reinhard F.HTTL Chair of Soil Protection and Recultivation Brandenburg University of Technology Helmholtz Centre Potsdam-German Research Centre for Geosciences Department of Renewable Resources University of Alberta

Nutrient-poor, sandy soils form the prevailing substrate at post-mining sites of the Lusatian region(Brandenburg, Germany) and present a challenge for vegetation development. We studied the organic acid quantity and composition of three commonly occurring pioneer plant species, the legumes Lotus corniculatus L. and trifolium arvense L. and the grass Calamagrostis epigeios(L.) Roth, to determine if plant growth and exudation differed with(non-sterilized soil) and without(sterilized soil) an indigenous soil microbial community. We investigated whether organic acids were found in the rhizosphere and surrounding soil and whether this influenced nutrient mobilization. This study consists of linked field investigations and a greenhouse experiment. Plants were grown in the greenhouse in either sterilized or non-sterilized sandy soil from a reclamation site in the Lusatian mining landscape(Welzow Su¨d, East Germany). After seven months, the plant biomass, root morphology, organic acids, and water-soluble nutrients and root colonization with arbuscular mycorrhizal fungi(AMF) and dark septate endophytes(DSE) were analyzed. Roots of all three plants in the field and greenhouse experiments were highly colonized with AMF. Calamagrostis epigeios and T. arvense had a significantly higher colonization frequency with DSE than L. corniculatus. The quantity and composition of organic acids strongly differed among plant species, with the highest number of organic acids found for L. corniculatus and lowest for C. epigeios. The quantity of organic acids was greatly reduced in all plants under sterilized soil conditions. However, the composition of organic acids and plant growth in sterilized soil were reduced for both legumes, but not for C. epigeios, which had a higher biomass under sterilized conditions. Changes in nutrient concentrations in the field rhizosphere soil relative to those in the control were measurable after seven months. While the spectrum of organic acids and the growth

关键词： Calamagrostis epigeios indigenous soil microbial community Lotus corniculatus microbial colonization mining landscape nutrient mobilization root symbiotic partner trifolium arvense

Chemical Forms and Phytoavailability of Copper in Soil as Affected by Crop Residues Incorporation

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American Journal of Analytical Chemistry 2014年第9期5卷 604-612页

作者： Shahrzad Kabirinejad Mahmoud Kalbasi Amir Hossein Khoshgoftarmanesh Mehran Hoodaji Majid Afyuni College of Agriculture Isfahan (Khorasgan) Branch Islamic Azad University Isfahan Iran College of Agriculture Isfahan University of Technology Isfahan Iran

Preceding crops as a source of organic matter are important sources of micronutrient and can play an important role in the soil fertility and soil cycling of micronutrients. In addition to the role of the organic matter in increasing the concentration of micronutrients in soil solution, attention should be also paid to the role of the kind and the quantity of the root’s exudates released in response to the incorporation of different plant residues in the rhizosphere. Present research was conducted with the objective of studying the effect of the kind of preceding crops: trifolium (trifolium pretense L) and Sorghum (Sorghum bicolor L) on chemical forms of copper (Cu) in solid phases of a calcareous soil in a completely randomized block field experiment with split plot (3 m × 5 m) arrangement, consisting of 3 replications and 3 treatments. After incorporation of the residue, wheat (genotype back cross) was planted. After harvesting the wheat, soil samples were collected from root zone of wheat. Selected soil properties and chemical forms of Cu were determined in the solid phases of the soil samples. Incorporation of plant residues significantly increased the concentration of DTPA-extractable Cu, in the soil. The highest effect was obtained for trifolium treatment. Incorporation of plant residues decreased the carbonate-bound Cu (Cu-Carb) fraction in the solid phase and increased oxide-bound Cu (Cu-Ox) as compared to the control (fallow treatment). Fraction of organic-bound Cu (Cu-Org) in the soil increased with incorporation of plant residues as compared with the fallow treatment. trifolium was the most effective in increasing Cu-Org. Cu-Ox and Cu-Residual (Cu-Res) forms showed a significant negative correlation and Cu-Org showed a significant positive correlation with the concentration of DTPA-extractable Cu. Incorporation of trifolium residues decreased the fraction (%) of Cu-Carb and Cu-Ox (less soluble forms) and consequently increased the fraction (%) of Cu-Org w

关键词： Crop Residues Copper Fractionation trifolium Sorghum DTPA-Extractable Cu

Development, Reproduction and Body Size Variation of Aphis glycines Matsumura Fed on Different Plants

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Journal of Northeast Agricultural University(English Edition) 2019年第3期26卷 27-35页

作者： Liu Jian Wang Su-ji Bai Bing Gao Bo Fan Yan-jie College of Agriculture Northeast Agricultural University

Soybean aphid, Aphis glycines Matsumura is one of the most important pests in soybean. Life cycle of A. glycines is characterized as heteroecious and holocyclic. The primary hosts of A. glycines are Rhamnus spp. and the secondary hosts include cultivated soybean, Glycine max(L.) Merr, and wild soybean, Glycine soja Sieb.& Zucc. In this study, A. glycines were fed on trifolium repens L. and Metaplexis japonica(Thunb.) Makino for three generations and their development, reproduction and body sizes were studied. These data were compared to the control fed on the known hosts, G. max and G. soja. These newly deposited offspring by the 3 rd generation were transferred back onto G. max and these differences in their development, reproduction and body sizes were also studied. It showed that A. glycines all could survive, develop and reproduce well, when they were fed on T. repens and M. japonica for three generations, but there were significant differences in their nymph stage, adult longevity and fecundity, life table parameters and body sizes. When these offspring were transferred back onto G. max which were newly deposited by the 3 rd generation aphids fed on T. repens and M. japonica, they could survive, develop and reproduce well, but there were significant differences in their nymph stage, adult longevity, intrinsic rate of increase and body sizes. It provided important information for studies on host adaptability of A. glycines on T. repens and M. japonica and to make clear the potential that this aphid involved into host biotypes on these plants.

关键词： Aphis glycines Matsumura trifolium repens L. Metaplexis japonica (Thunb.) Makino

Genetic structure and diversity of Ukrainian red clover cultivars revealed by microsatellite markers

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Open Journal of Genetics 2013年第4期3卷 235-242页

作者： Yulia N. Dugar Vitalii N. Popov Kharkov National Agrarian University nd. a. V. V. Dokuchaev Post Office Communist-1 Kharkov District Ukraine Рlant Production Institute nd. a. V. Ya. Yuryev of National Academy of Agrarian Science Kharkov Ukraine

Polymorphism of microsatellite loci of Ukrainian red clover cultivars has been studied. 87 microsatellite alleles, which occurred in different combinations, were identified. The number of alleles ranged from 7 to 10. Microsatellite allele distribution showed that 15 alleles were common for all the red clover cultivars (17.2%). The red clover cultivars were represented by homozygous and heterozygous genotypes. The observed and expected heterozygosity ranged from 0.067 to 0.269 and from 0.225 to 0.807, respectively. An analysis of molecular variance revealed that the largest proportion of variation (68.5%) resided at the intrapopulation level. Differentiation of the Ukrainian cultivars was moderately expressed (FST = 0.07).

关键词： Genetic Structure Heterozygosity Microsatellites Polymorphism trifolium pratense L.

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白三叶在湖北省的生长与利用

中国草地学报 1989年第3期26卷 57-59页

作者：鲍健寅聂中南湖北省农科院畜牧兽医研究所

白三叶(trifolium repens L.)属温带多年生牧草,是新西兰、澳大利亚草地中主要豆科牧草之一,在美国、英国、法国、荷兰、丹麦、巴西等地有广泛分布,我国贵州、云南、江西、四川、新疆、湖北等地都有其野生种分布。

关键词：白三叶牧草品种 trifolium 多年生牧草种子产量野生种越冬率人工草地建设产草量低海拔地区

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发酵红三叶草对肉仔鸡公鸡生产性能的研究

黑龙江畜牧兽医 2014年第11期 114-116页

作者：孟翠红张福元山西运城农业职业技术学院山西运城044000 山西农业大学动物科学院山西太谷030800

红三叶（red clover）为豆科多年生草本植物,属车轴草属（trifolium L.）,在我国新疆、吉林、云贵高原、北鄂西山地都有野生,江淮流域、华北、华南、西南等地均有栽培[1],主要含异黄酮、维生素、氨基酸、糖类等多种生物活性成分,其中异... 详细信息

红三叶（red clover）为豆科多年生草本植物,属车轴草属（trifolium L.）,在我国新疆、吉林、云贵高原、北鄂西山地都有野生,江淮流域、华北、华南、西南等地均有栽培[1],主要含异黄酮、维生素、氨基酸、糖类等多种生物活性成分,其中异黄酮是红三叶草中特殊的活性成分[2]。异黄酮具有多种生理活性,概括起来主要有两个方面,即植物雌激素功能和抗氧化功能[3]。

关键词：植物雌激素鄂西山地抗氧化功能车轴草属固态发酵活性成分生产性能 trifolium 日增重日采食量