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Phenotype and metabolism alterations in PCB-degrading Rhodococcus biphenylivorans TG9^(T) under acid stress

Journal of Environmental Sciences 2023年第5期127卷 441-452页

作者： Aili Li Jiahui Fan Yangyang Jia Xianjin Tang Jingwen Chen Chaofeng Shen Department of Environmental Engineering College of Environmental and Resource SciencesZhejiang UniversityHangzhou 310058China MOE Key Lab of Environmental Remediation and Ecosystem Health College of Environmental and Resource SciencesZhejiang UniversityHangzhou 310058China

Environmental acidification impairs microorganism diversity and their functions on substance *** is a ubiquitously distributed genus for contaminant detoxification in the environment,and it can also adapt a certain range of *** work interpreted the acid responses from both phenotype and metabolism in strain Rhodococcus biphenylivorans TG9^(T)(TG9)induced at pH *** phenotype alterations were described with the number of culturable and viable cells,intracellular ATP concentrations,cell shape and entocyte,degradation efficiency of polychlorinated biphenyl(PCB)31 and *** number of culturable cells maintained rather stable within the first 10 days,even though the other phenotypes had noticeable alterations,indicating that TG9 possesses certain capacities to survive under acid *** metabolism responses were interpreted based on transcription analyses with four treatments including log phase(LP),acid-induced(PER),early recovery after removing acid(RE)and later recovery(REL).With the overview on the expression regulations among the 4 treatments,the RE sample presented more upregulated and less downregulated genes,suggesting that its metabolism was somehow more active after recovering from acid *** addition,the response mechanism was interpreted on 10 individual metabolism pathways mainly covering protein modification,antioxidation,antipermeability,H+consumption,neutralization and ***,the transcription variations were verified with RT-qPCR on 8 genes with 24-hr,48-hr and 72-hr acid *** together,TG9 possesses comprehensive metabolism strategies defending against acid ***,a model was built to provide an integrate insight to understand the acid resistance/tolerance metabolisms in microorganisms.

关键词： Environmental acidification Rhodococcus biphenylivorans Contaminant degradation Phenotypic response transcription analysis Metabolism pathway Mechanism model

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Induced Deactivation of Genes Encoding Chlorophyll Biosynthesis Enzymes Disentangles Tetrapyrrole- Mediated Retrograde Signaling

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Molecular Plant 2014年第7期7卷 1211-1227页

作者： Hagen Schlicke Annabel Salinas Hartwig Vivien Firtzlaff Andreas S. Richter , Christine Glasser Klaus Maier Iris Finkemeier Bernhard Grimm Institute of Biology/Plant Physiology Humboldt University Berlin Philippstr. 13 Building 12 D 10115 Berlin Germany Helmholtz Zentrum Monchen Deutsches Forschungszentrum for Gesundheit und Umwelt (GmbH) Ingolstadter Landstr. 1 85764 Neuherberg Germany Max-Planck-lnstitute for Plant Breeding Research. Plant Proteomics and Mass Spectrometry Group Carl-von-Linne Weg 10 50829 Cologne Germany

In photosynthetic organisms, tetrapyrrole-mediated retrograde signals are proposed to contribute to a bal- anced nuclear gene expression （NGE） in response to metabolic activity in chloroplasts. We followed an experimental short- term approach that allowed the assessment of modified NGE during the first hours of specifically modified enzymatic steps of the Mg branch of tetrapyrrole biosynthesis, when pleiotropic effects of other signals can be avoided. In response to 24-h-induced silencing of CHLH, CHLM, and CHL27 encoding the CHLH subunit of Mg chelatase, the Mg protoporphyrin methyltransferase and Mg protoporphyrin monomethylester cyclase, respectively, deactivated gene expression rapidly led to reduced activity of the corresponding enzymes and altered Mg porphyrin levels. But NGE was not substantially altered. When these three genes were continuously inactivated for up to 4 d, changes of transcript levels of nuclear genes were determined. CHL27 silencing for more than 24h results in necrotic leaf lesions and modulated transcript levels of oxidative stress-responsive and photosynthesis-associated nuclear genes （PhANGs）. The prolonged deactivation of CHLH and CHLM results in slightly elevated transcript levels of PhANGs and tetrapyrrole-associated genes. These time-resolved studies indicate a complex scenario for the contribution of tetrapyrrole biosynthesis on NGE mediated by IO2-induced signaling and feedback-regulated ALA synthesis.

关键词： plastid signal transcription analysis retrograde signal tetrapyrrole metabolism Mg protoporphyrin.

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Antimicrobial mechanisms of g-C3N4 nanosheets against the oomycetes Phytophthora capsici:disrupting metabolism and membrane structures and inhibiting vegetative and reproductive growth

Antimicrobial mechanisms of g-C3N4 nanosheets against the oo...

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中国植物病理学会2021年学术年会

作者：蔡璘 WEI Xuefeng FENG Hui FAN Guangjin GAO Changdan WANG Daibin RAN Mao XU Chen XU Xiaohong 孙现超 College of Plant Protection Southwest University

To understand the potential of urea-synthesized g-C3 N4 nanosheets（0.125～1 mg/mL） as antimicrobial agents against oomycetes,an investigation of the interaction mechanism between g-C3 N4 nanosheets and Phytophthora capsici was *** analysis showed that after being exposed to g-C3 N4 nanosheets for 1 h,*** triggered a sharp upregulation of antioxidant activities and structural constituents and a downregulation of metabolic pathways,including ATP generation,autophagy disruption,membrane system disorders and other complex adaptive *** the life stages of ***,including mycelial growth,sporangium formation,zoospore numbers and zoospore germination were remarkably inhibited and even injured.A mutual mechanism is proposed in this work:ROS stress upon exposure to visible irradiation and,combined with their sharp nanosheet structure,cause perturbations of the cell membrane and induce damage to the ultrastructure of mycelial growth,sporangium and *** that the antimicrobial action of g-C3 N4 nanosheets were derived from the damage throughout the duration of treatment and was not limited to a single target,these complex mechanisms could favor the avoidance of drug resistance and benefit other oomycetes *** importantly,in addition to restraining *** infection in host plants,g-C3 N4 nanosheets promoted pepper plant ***,g-C3 N4 nanosheets have potential as a new non-metal antimicrobial agent to control oomycotal disease in crops.

关键词： g-C3N4 nanosheets Antimicrobial mechanisms Phytophthora capsici transcription analysis

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