Phenotype and metabolism alterations in PCB-degrading Rhodococcus biphenylivorans TG9^(T) under acid stress

作     者：Aili Li Jiahui Fan Yangyang Jia Xianjin Tang Jingwen Chen Chaofeng Shen Aili Li;Jiahui Fan;Yangyang Jia;Xianjin Tang;Jingwen Chen;Chaofeng Shen

作者机构：Department of Environmental EngineeringCollege of Environmental and Resource SciencesZhejiang UniversityHangzhou 310058China MOE Key Lab of Environmental Remediation and Ecosystem HealthCollege of Environmental and Resource SciencesZhejiang UniversityHangzhou 310058China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2023年第127卷第5期

页      面：441-452页

核心收录：

学科分类：07[理学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported by the National Key Research and Development Program of China(No.2019YFC1803700) the National Natural Science Foundation of China(Nos.21876149 and 42077125) 

主　　题：Environmental acidification Rhodococcus biphenylivorans Contaminant degradation Phenotypic response Transcription analysis Metabolism pathway Mechanism model 

摘      要：Environmental acidification impairs microorganism diversity and their functions on substance *** is a ubiquitously distributed genus for contaminant detoxification in the environment,and it can also adapt a certain range of *** work interpreted the acid responses from both phenotype and metabolism in strain Rhodococcus biphenylivorans TG9^(T)(TG9)induced at pH *** phenotype alterations were described with the number of culturable and viable cells,intracellular ATP concentrations,cell shape and entocyte,degradation efficiency of polychlorinated biphenyl(PCB)31 and *** number of culturable cells maintained rather stable within the first 10 days,even though the other phenotypes had noticeable alterations,indicating that TG9 possesses certain capacities to survive under acid *** metabolism responses were interpreted based on transcription analyses with four treatments including log phase(LP),acid-induced(PER),early recovery after removing acid(RE)and later recovery(REL).With the overview on the expression regulations among the 4 treatments,the RE sample presented more upregulated and less downregulated genes,suggesting that its metabolism was somehow more active after recovering from acid *** addition,the response mechanism was interpreted on 10 individual metabolism pathways mainly covering protein modification,antioxidation,antipermeability,H+consumption,neutralization and ***,the transcription variations were verified with RT-qPCR on 8 genes with 24-hr,48-hr and 72-hr acid *** together,TG9 possesses comprehensive metabolism strategies defending against acid ***,a model was built to provide an integrate insight to understand the acid resistance/tolerance metabolisms in microorganisms.