检索结果-南通市图书馆

Molecular Plant 2009年第6期2卷 1289-1297页

作者： Marco Schottkowski Janina Ratke Ulrike Oster Marc Nowaczyk Jorg Nickelsen Molekulare Pflanzenwissenschaften Biozentrum LMU Munchen Groβhaderner Str. 2-4 82152 Planegg-Martinsried Germany Biochemie und Physiologie der Pflanzen Biozentrum LMU Munchen Groβhaderner Str. 2-4 82152 Planegg-Martinsried Germany LS Biochemie der Pflanzen Ruhr-Universitait Bochum Universitatsstr. 150 44780 Bochum Germany

Biogenesis of photosynthetic pigment/protein complexes is a highly regulated process that requires various assisting factors. Here, we report on the molecular analysis of the Pitt gene （sir1644） from the cyanobacterium Synechocystis sp. PCC 6803 （Synechocystis 6803） that encodes a membrane-bound tetratricopeptide repeat （TPR） protein of formerly unknown function. Targeted inactivation of Pitt affected photosynthetic performance and light-dependent chlorophyll synthesis. Yeast two-hybrid analyses and native PAGE strongly suggest a complex formation between Pitt and the light-dependent protochlorophyllide oxidoreductase （POR）. Consistently, POR levels are approximately threefold reduced in the pitt insertion mutant. The membrane sublocalization of Pitt was found to be dependent on the presence of the periplasmic photosystem Ⅱ （PSⅡ） biogenesis factor PratA, supporting the idea that Pitt is involved in the early steps of photosynthetic pigment/protein complex formation.

关键词： Synechocystis TPR protein POR thylakoid membrane chlorophyll.

Overaccumulation of glycine betaine makes the function of the thylakoid membrane better in wheat under salt stress

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Crop Journal 2017年第1期5卷 73-82页

作者： Fengxia Tian Wenqiang Wang Chao Liang Xin Wang Guiping Wang Wei Wang State Key Laboratory of Crop Biology Shandong Key Laboratory of Crop BiologyCollege of Life SciencesShandong Agricultural University College of Life Science and Technology Nanyang Normal University

Wheat(Triticum aestivum L.) lines T1, T4, and T6 were genetically modified to increase glycine betaine(GB) synthesis by introduction of the BADH(betaine aldehyde dehydrogenase, BADH)gene from mountain spinach(Atriplex hortensis L.). These transgenic lines and WT of wheat(T. aestivum L.) were used to study the effect of increased GB synthesis on wheat tolerance to salt stress. Salt stress due to 200 mmol L-1Na Cl impaired the photosynthesis of the four wheat lines, as indicated by declines in net photosynthetic rate(Pn), stomatal conductance(Gs),maximum photochemical efficiency of PSII(Fv/Fm), and actual photochemical efficiency of PSII(ФPSII) and an increase in intercellular CO2concentration(Ci). In comparison with WT, the effect of salinity on the three transgenic lines was mild. Salt stress caused disadvantageous changes in lipids and their fatty acid compositions in the thylakoid membrane of the transgenic lines and WT. Under salt stress, the three transgenic lines showed slightly higher chlorophyll and carotenoid contents and higher Hill reaction activities and Ca2+-ATPase activity than WT. All the results suggest that overaccumulation of GB resulting from introduction of the BADH gene can enhance the salt tolerance of transgenic plants, especially in the protection of the components and function of thylakoid membranes, thereby making photosynthesis better. Changes in lipids and fatty acid compositions in the thylakoid membrane may be involved in the increased salt stress tolerance of the transgenic lines.

关键词： Transgenic wheat Glycine betaine Photosynthesis Salt stress thylakoid membrane

A thylakoid membrane Protein Functions Synergistically with GUN5 in Chlorophyll Biosynthesis

在线全文

学校读者我要写书评

暂无评论

Plant Communications 2020年第5期1卷 100-110页

作者： Chi Zhang Bin Zhang Baicong Mu Xiaojiang Zheng Fugeng Zhao Wenzhi Lan Aigen Fu Sheng Luan The Key Laboratory of Western Resources Biology and Biological Technology College of Life SciencesNorthwest UniversityXi’an 710069China Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology College of Life SciencesNanjing UniversityNanjing 210093China Temasek Life Sciences Laboratory Singapore 117604Republic of Singapore Department of Plant and Microbial Biology University of CaliforniaBerkeleyCA 94720USA

Chlorophyll(Chl)is essential for photosynthetic reactions and chloroplast *** the enzymatic pathway for Chl biosynthesis is well established,the regulatory mechanism underlying the homeostasis of Chl levels remains largely *** this study,we identified CBD1(Chlorophyll Biosynthetic Defect1),which functions in the regulation of chlorophyll *** CBD1 gene was expressed specifically in green tissues and its protein product was embedded in the thylakoid ***,CBD1 was precisely co-expressed and functionally correlated with GUN5(Genome Uncoupled 5).Analysis of chlorophyll metabolic intermediates indicated that cbd1 and cbd1gun5 mutants over-accumulatedmagnesium protoporphyrin Ⅸ(Mg-Proto Ⅸ).In addition,the cbd1 mutant thylakoid contained less Mg than the wild type not only as a result of lower Chl content,but also implicating CBD1 in Mg *** was supported by the finding that CBD1 complemented a Mg^(2+)uptake-deficient Salmonella strain under low Mg *** together,these results indicate that CBD1 functions synergistically with CHLH/GUN5 in Mg-Proto IX processing,and may serve as a Mg-transport protein to maintain Mg homeostasis in the chloroplast.

关键词： chlorophyll biosynthesis magnesium thylakoid membrane GUN5 magnesium protoporphyrin

A Novel Two Domain-Fusion Protein in Cyanobacteria with Similarity to the CAB/ELIP/HLIP Superfamily： Evolutionary Implications and Regulation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2008年第1期1卷 155-166页

作者： Oliver Kilian Anne Soisig Steunou Arthur R. Grossman Devaki Bhaya Department of Plant Biology The Carnegie Institution Stanford CA 94305 USA

Vascular plants contain abundant, light-harvesting complexes in the thylakoid membrane that are non-covalently associated with chlorophylls and carotenoids. These light-harvesting chlorophyll a/b binding （LHC） proteins are members of an extended CAB/ELIP/HLIP superfamily of distantly related polypeptides, which have between one and four transmembrane helices （TMH）. This superfamUy includes the single TMH, high-light-inducible proteins （Hlips）, found in cyanobacteria that are induced by various stress conditions, including high light, and are considered ancestral to the LHC proteins. The roles of, and evolutionary relationships between, these superfamily members are of particular interest, since they function in both light harvesting and photoprotection and may have evolved through tandem gene duplication and fusion events. We have investigated the Hlips （hli gene family） in the thermophilic unicellular cyanobacterium Synechococcus OS-B＇. The five hli genes present on the genome of Synechococcus OS-B＂ are relatively similar, but transcript analyses indicate that there are different patterns of transcript accumulation when the cells are exposed to various growth conditions, suggesting that different Hlips may have specific functions. Hlip5 has an additional TMH at the N-terminus as a result of a novel fusion event. This additional TMH is very similar to a conserved hypothetical, single membrane-spanning polypeptide present in most cyanobacteria. The evolutionary significance of these results is discussed.

关键词： fusion proteins Cyanobacteria N-Terminus stress conditions Large Hadron Collider Growth conditions photostability Regulation (biology) thylakoid membrane Tracheobionta gene duplication Analogy superfamily Chlorophyll Evolutionary

Towards Characterization of the Chloroplast NAD（P）H Dehydrogenase Complex

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2009年第6期2卷 1127-1140页

作者： Marjaana Suorsa Sari Sirpio Eva-Mari Aro Department of Biology Plant Physiology and Molecular Biology University of Turku FI-20014 Turku Finland

The NAD（P）H dehydrogenase （NDH） complex in chloroplast thylakoid membranes functions in cyclic electron transfer, and in chlororespiration. NDH is composed of at least 15 subunits, including both chloroplast- and nuclear-encoded proteins. During the past few years, extensive proteomic and genetic research on the higher plant NDH complex has been carried out, resulting in identification of several novel nuclear-encoded subunits. In addition, a number of auxiliary proteins, which mainly regulate the expression of chloroplast-encoded ndh genes as well as the assembly and stabilization of the NDH complex, have been discovered and characterized. In the absence of detailed crystallographic data, the structure of the NDH complex has remained obscure, and therefore the role of several NDH-associated nuclear-encoded proteins either as auxiliary proteins or structural subunits remains uncertain. In this review, we summarize the current knowledge on the subunit composition and assembly process of the chloroplast NDH complex. In addition, a novel oligomeric structure of NDH, the PSI/NDH supercomplex, is discussed.

关键词： Arabidopsis maize tobacco NAD(P)H dehydrogenese chloroplast thylakoid membrane cyclic electron transfer photosystem I.

Dissecting the Native Architecture and Dynamics of Cyanobacterial Photosynthetic Machinery

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2017年第11期10卷 1434-1448页

作者： Selene Casella Fang Huang David Mason Guo-Yan Zhao Giles N. Johnson Conrad W. Mullineaux Lu-Ning Liu Institute of Integrative Biology University of Liverpool Crown Street Liverpool L69 7ZB UK Centre for Cell Imaging University of Liverpool Crown Street Liverpool L69 7ZB UK College of Life Science Shandong Normal University Jinan 250014 P. R. China School of Earth and Environmental Sciences University of Manchester Oxford Road Manchester M13 9PT UK School of Biological and Chemical Sciences Queen Mary University of London Mile End Road London E1 4NS UK

The structural dynamics and flexibility of cell membranes play fundamental roles in the functions of the cells, i.e., signaling, energy transduction, and physiological adaptation. The cyanobacterial thylakoid membrane represents a model membrane that can conduct both oxygenic photosynthesis and respiration simultaneously. In this study, we conducted direct visualization of the global organization and mobility of photosynthetic complexes in thylakoid membranes from a model cyanobacterium, Synechococcus elongatus PCC 7942, using high-resolution atomic force, confocal, and total internal reflection fluorescence microscopy. We visualized the native arrangement and dense packing of photosystem I （PSI）, photosystem II （PSlI）, and cytochrome （Cyt） befwithin thylakoid membranes at the molecular level. Furthermore, we func- tionally tagged PSI, PSlI, Cyt bef, and ATP synthase individually with fluorescent proteins, and revealed the heterogeneous distribution of these four photosynthetic complexes and determined their dynamic features within the crowding membrane environment using live-cell fluorescence imaging. We characterized red light-induced clustering localization and adjustable diffusion of photosynthetic complexes in thylakoid membranes, representative of the reorganization of photosynthetic apparatus in response to environmental changes. Understanding the organization and dynamics of photosynthetic membranes is essential for rational design and construction of artificial photosynthetic systems to undarpin bioenergy development. Knowledge of cyanobacterial thylakoid membranes could also be extended to other cell membranes, such as chloroplast and mitochondrial membranes.

关键词： cyanobacteria thylakoid membrane atomic force microscopy photosynthesis fluorescence imaging protein dynamics

Induction of Apoptosis in Purified Nuclei from Tobacco-Suspension Cells by Cytochrome b_6/f Complex

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tsinghua Science and Technology 2004年第4期9卷 391-396页

作者：张贵友李萍朱瑞宇田瑞华戴尧仁 Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084China

An apoptotic cell-free system containing cytosol and nuclei from normally cultured tobacco sus-pension cells was used to show that a spinach chloroplast preparation can induce apoptosis in nuclei, evi-denced by DNA electrophoresis and fluorescence microscopy observations. Further study showed that the chloroplast preparation or its pellet (thylakoid membrane) after hypoosmotic or supersonic treatment still ex-hibited the apoptosis-inducing activity, but the supernatant had no effect, which indicates that the apoptosis-inducing effector in the chloroplast preparation is water-insoluble. The induction of apoptosis by chloroplast preparation could be attenuated by Ac-DEVD-CHO, the specific inhibitor of Caspase-3, implying involve-ment of a Caspase-3-like protease during the process. Furthermore, extensive apoptosis in nuclei was in-duced by cytochrome b6/f on the thylakoid membrane, indicating that this important cytochrome complex may have an important role in the chloroplast-related apoptotic pathway.

关键词： apoptosis chloroplast cytosol thylakoid membrane cytochrome b6 / f complex

Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第7期18卷 635-648页

作者： Tao LU Jie-wei SHI Zhou-ping SUN Ming-fang QI Yu-feng LIU Tian-lai LI College of Horticulture Shenyang Agricultural University Shenyang 110866 China Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province Shenyang 110866 China Collaborative Innovation Center of Protected Vegetable Surrounds Bohai Gulf Region Shenyang 110866 China

Objective： To evaluate the possible photoprotection mechanisms of cyclic and linear electron flux （CEF and LEF） under specific high temperature and high light （HH） stress. Methods： Six-leaf-stage tomato seedlings （＂Liaoyuanduoli＂, n=160） were divided into four parts： Part 1, served as control under 25 ℃, 500 pmol/（m2-s）; Part 2, spayed with distilled water （H20） under 35 ℃, 1000 pmol/（m2.s）（HH）; Part 3, spayed with 100 μmol/L diuron （DCMU, CEF inhibitor） under HH; Part 4, spayed with 60 pmol/L methyl viologen （MV, LEF inhibitor） under HH. Energy conversion, photosystem I （PSI）, and PSII activity, and trans-thylakoid membrane proton motive force were monitored during the treatment of 5 d and of the recovering 10 d. Results： HH decreased photochemical reaction dissipation （P） and the maximal photochemical efficiency of PSII （Fv/Fm）, and increased the excitation energy distribution coefficient of PSII （,8）; DCMU and MV aggravated the partition imbalance of the excitation energy （y） and the photoinhibition degree. With prolonged DCMU treatment time, electron transport rate and quantum efficiency of PSI （ETR= and Y1） significantly decreased whereas acceptor and donor side limitation of PSI （YNA and YND） increased. MV led to a significant decline and accession of yield of regulated and non-regulated energy Y NPQ and YNO, respectively. membrane integrity and ATPase activity were reduced by HH stress, and DCMU and MV enhanced inhibitory actions. Conclusions： The protective effects of CEF and LEF were mediated to a certain degree by meliorations in energy absorption and distribution as well as by maintenance of thylakoid membrane integrity and ATPase activity.

关键词： Photoinhibition Photoprotection thylakoid membrane Tomato

Chloroplast Composition and Structural Differences in a Chlorophyll-reduced Mutant of Oilseed Rape Seedlings

在线全文

学校读者我要写书评

暂无评论

Acta Botanica Sinica 2001年第8期43卷 877-880页

作者：赵云杜林方杨胜洪李世崇张义正四川大学生命科学学院成都610064

对黄化突变体Cr352 9和野生型油菜 (BrassicanapusL .) 352 9叶绿体的超微结构和组成进行了比较。与野生型相比 ,突变体Cr352 9叶片具有较少的类囊体、较少的垛叠膜区和较少的叶绿素含量。突变体的Chla/Chlb比值较高 ,是野生型的 2倍。... 详细信息

对黄化突变体Cr352 9和野生型油菜 (BrassicanapusL .) 352 9叶绿体的超微结构和组成进行了比较。与野生型相比 ,突变体Cr352 9叶片具有较少的类囊体、较少的垛叠膜区和较少的叶绿素含量。突变体的Chla/Chlb比值较高 ,是野生型的 2倍。电泳结果表明 ,突变体类囊体膜中LHCⅡ和其三聚体LHCⅡ 的含量减少。SDS_PAGE分析显示 ,LHCⅡ的脱辅基蛋白在突变体类囊体膜中明显减少。免疫印迹进一步表明 ,所有LHCⅡ组分的含量仅为野生油菜的类囊体膜的 1 / 3。突变体Cr352 9的天线系统比野生型 352 9的小。

关键词： oilseed rape thylakoid membrane pigment protein chlorophyll_reduced mutant