Pitt, a Novel Tetratricopeptide Repeat Protein Involved in Light-Dependent Chlorophyll Biosynthesis and Thylakoid Membrane Biogenesis in Synechocystis sp. PCC 6803

作     者：Marco Schottkowski Janina Ratke Ulrike Oster Marc Nowaczyk Jorg Nickelsen 

作者机构：Molekulare Pflanzenwissenschaften Biozentrum LMU Munchen Groβhaderner Str. 2-4 82152 Planegg-Martinsried Germany Biochemie und Physiologie der Pflanzen Biozentrum LMU Munchen Groβhaderner Str. 2-4 82152 Planegg-Martinsried Germany LS Biochemie der Pflanzen Ruhr-Universitait Bochum Universitatsstr. 150 44780 Bochum Germany 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2009年第2卷第6期

页      面：1289-1297页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0903[农学-农业资源与环境] 090302[农学-植物营养学] 

基　　金：德国德意志研究联合会(DFG)资助 

主　　题：Synechocystis TPR protein POR thylakoid membrane chlorophyll. 

摘      要：Biogenesis of photosynthetic pigment/protein complexes is a highly regulated process that requires various assisting factors. Here, we report on the molecular analysis of the Pitt gene （sir1644） from the cyanobacterium Synechocystis sp. PCC 6803 （Synechocystis 6803） that encodes a membrane-bound tetratricopeptide repeat （TPR） protein of formerly unknown function. Targeted inactivation of Pitt affected photosynthetic performance and light-dependent chlorophyll synthesis. Yeast two-hybrid analyses and native PAGE strongly suggest a complex formation between Pitt and the light-dependent protochlorophyllide oxidoreductase （POR）. Consistently, POR levels are approximately threefold reduced in the pitt insertion mutant. The membrane sublocalization of Pitt was found to be dependent on the presence of the periplasmic photosystem Ⅱ （PSⅡ） biogenesis factor PratA, supporting the idea that Pitt is involved in the early steps of photosynthetic pigment/protein complex formation.