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On Subversion of Traditional Male and Female Images in Angela Cater's Modern Fairy tales

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海外英语 2020年第5期 192-197,201页

作者：陶羽洁上海师范大学人文与传播学院上海200234

Angela Carter is a famous contemporary British female writer with a variety of creative styles, famous for Gothic style, magic realism and new feminism. As one of the representatives of feminist writers in the last century, Angela Carter devoted her life to prosecuting patriarchal society from the perspective of women, calling on women to find themselves and realize personality libera-tion. At the same time, she is also a rational feminist, advocating the establishment of harmonious rather than antagonistic social re-lations with men and *** article will mainly use text analysis and contrast and comparison to study the image of the male and female image in Angela Carter's modern fairy tale, and explore the reasons. On the basis of the text analysis, this paper will study the transformation of female image from the change of female status and personality. The laws of comparison and comparison will be used to explore the differences between men and women in the traditional fairy tale and the modern fairy tale of Angela Cart-er. The study found that in Carter's reconstruction of the fairy tale, the female image dares to break through the inherent status, and constantly launches the impact on the men's rights, seeking individual independence and self-liberation, and the male image is more diversified and more realistic and vivid. Through this study, it is hoped that the reader will have a deeper and more compre-hensive understanding of Angela Carter and his work, and find out the new feminist connotation contained in his work, thus ending the dualistic confrontation of the male and female and establishing a harmonious relationship.

关键词： subversion traditional male and female image Angela Carter modern fairy tale

Subverting the Traditional Function of Fairy tales

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海外英语 2012年第1X期 191-192页

作者：李冬艳榆林学院外语系陕西榆林719000

Through analyzing Carter' s The Bloody Chamber and Other Stories,it is meant to expose the fact that the traditional function of fairy tales,namely the vehicle to enslave and indoctrinate children,has been subverted as a " loudspeaker" for long restrained,voiceless fe males to speak out their rights and identities,so as to dismantle the deeply-rooted patriarchy.

关键词： fairy tale function subversion

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甜橙Cstale家族基因鉴定与表达分析

甜橙CsTALE家族基因鉴定与表达分析

作者：杨洋湖南农业大学

学位级别：硕士

Three-amino-acid-loop-extension(tale)家族是植物中最重要的转录因子家族之一,主要在调控植物生长发育、参与植物应激反应等方面发挥着重要的作用。尽管甜橙(Citrus sinensis)是世界上最具商业价值的水果作物之一,但对该物种的tale基... 详细信息

Three-amino-acid-loop-extension(tale)家族是植物中最重要的转录因子家族之一,主要在调控植物生长发育、参与植物应激反应等方面发挥着重要的作用。尽管甜橙(Citrus sinensis)是世界上最具商业价值的水果作物之一,但对该物种的tale基因的功能研究相对较少。在本研究中,对甜橙Cstale家族基因18个成员进行鉴定与表达分析。本研究可以为阐明甜橙中tale家族基因的生物学功能奠定基础。主要结果如下:1.从甜橙基因组中鉴定到18个Cstale基因,通过与拟南芥、杨树与水稻中的tale基因构建系统发育树,发现18个Cstale基因主要分为两个亚家族,即KNOX和BEL。2.根据在染色体上的位置分析发现,除了9号染色体之外,18个Cstale基因在所有染色体上均有分布。并且在3号染色体上,Cstale4和Cstale5发生了串联重复事件。进一步利用共线性和重复对分析发现,10个Cstale基因具有节段性重复。除了4号和10号染色体之外,其他染色体都发生了节段性重复,Cstale3、Cstale9、Cstale11这三个基因分别发生了三个重复事件。与模式植物拟南芥之间共鉴定出15个共线基因对。***两个亚家族都含有Homeobox＿KN结构域。而POX结构域只出现BEL亚家族中,KNOX1和KNOX2结构域出现在KNOX亚家族。除此之外,还分析了基因结构以及保守基序。基因启动子区域的顺式元件分析显示了Cstale基因在甜橙发育、生物或非生物胁迫中具有潜在功能。4.为分析Cstale的组织特异性,对甜橙茎、叶、花和果实进行q RT-PCR检测,发现除Cstale12/14在所有组织中均表达量较低外,其他的Cstale基因都在不同的组织中有不同程度的表达。这也表明Cstale基因家族在调控甜橙的生长发育过程中功能具有多样性。***在不同的生物和非生物胁迫下具有不同的表达模式。Cstale基因在非生物胁迫中(盐、高温、低温和创伤),8个Cstale对盐胁迫有反应,10个Cstale响应高温胁迫,4个Cstale参与低温胁迫,4个Cstale对创伤有反应。在生物胁迫下,柑橘分别接种柑橘砂皮病菌(Diaporthe citri)和柑橘黄龙病菌(Candidatus Liberibacter asiaticus)后,8个Cstale基因在***接种后显著上调,5个Cstale基因被明显抑制;大多数Cstale基因在接种Clas后上调,而Cstale15/17的表达被强烈抑制。6.对Cstale家族蛋白Cstale3/10进行了亚细胞定位分析,结果显示Cstale3/10均定位在细胞核内。7.通过酵母自激活验证Cstale蛋白的转录活性,发现只有Cstale14具有转录活性,进一步进行酵母双杂交验证了Cstale3/Cstale8、Cstale3/Cstale11、Cstale10/Cstale12、Cstale14/Cstale8、Cstale14/Cstale11的相互作用关系。

关键词： tale 转录因子柑橘表达分析转录激活

Xa7, a new executor R gene that confers durable and broad-spectrum resistance to bacterial blight disease in rice

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Plant Communications 2021年第3期2卷 68-81页

作者： Xifeng Chen Pengcheng Liu Le Mei Xiaoling He Long Chen Hui Liu Shurong Shen Zhandong Ji Xixi Zheng Yuchen Zhang Zhenyu Gao Dali Zeng Qian Qian Bojun Ma College of Chemistry and Life Sciences Zhejiang Normal UniversityJinhua 321004China China National Rice Research Institute Chinese Academy of Agricultural SciencesHangzhou 310006China

Bacterial blight(BB)is a globally devastating rice disease caused by Xanthomonas oryzae ***(Xoo).The use of disease resistance(R)genes in rice breeding is an effective and economical strategy for the control of this ***,a majority of R genes lack durable resistance for long-term use under global warming ***,we report the isolation of a novel executor R gene,Xa7,that confers extremely durable,broad-spectrum,and heat-tolerant resistance to *** expression of Xa7 was induced by incompatible Xoo strains that secreted the transcription activator-like effector(tale)AvrXa7 or PthXo3,which recognized effector binding elements(EBEs)in the Xa7 ***,Xa7 induction was faster and stronger under high *** of Xa7 or co-transformation of Xa7 with avrXa7 triggered a hypersensitive response in *** expression of Xa7 activated a defense response in the absence of Xoo but inhibited the growth of transgenic rice *** addition,analysis of over 3000 rice varieties showed that the Xa7 locuswas found primarily in the indica and aus subgroups.A variation consisting of an 11-bp insertion and a base substitution(G to T)was found in EBEAvrXa7 in the tested varieties,resulting in a loss of Xa7 BB *** a decade of effort,we have identified an important BB resistance gene and characterized its distinctive interaction with Xoo strains;these findings will greatly facilitate research on the molecular mechanism of Xa7-mediated resistance and promote the use of this valuable gene in breeding.

关键词： Xa7 executor R gene durable resistance tale bacterial blight rice

HOXB13 mutations and binding partners in prostate development and cancer:Function,clinical significance, and future directions

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Genes & Diseases 2017年第2期4卷 75-87页

作者： Hannah Brechka Raj R.Bhanvadia Calvin VanOpstall Donald J.Vander Griend The Committee on Cancer Biology The University of ChicagoChicagoILUSA The Pritzker School of Medicine The University of ChicagoChicagoILUSA Department of Surgery Section of UrologyThe University of ChicagoChicagoILUSA

The recent and exciting discovery of germline HOXB13 mutations in familial prostate cancer has brought HOX signaling to the forefront of prostate cancer *** enhanced understanding of HOX signaling,and the co-factors regulating HOX protein specificity and transcriptional regulation,has the high potential to elucidate novel approaches to prevent,diagnose,stage,and treat prostate *** our understanding of HOX biology in prostate development and prostate cancer,basic research in developmental model systems as well as other tumor sites provides a mechanistic framework to inform future studies in prostate *** we describe our current understanding of HOX signaling in genitourinary development and cancer,current clinical data of HOXB13 mutations in multiple cancers including prostate cancer,and the role of HOX protein co-factors in development and *** data highlight numerous gaps in our understanding of HOX function in the prostate,and present numerous potentially impactful mechanistic and clinical opportunities for future investigation.

关键词： Androgen receptor HOXB13 HOXB13(G84E) MEIS1 MEIS2 PBX Prostate tale

Illuminating the structure and dynamics of chromatin by fluorescence labeling

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Frontiers in Biology 2017年第4期12卷 241-257页

作者： Shipeng Shao Lei Chang Yingping Hou Yujie Sun State Key Laboratory of Membrane Biology Biodynamic Optical Imaging Center (BIOPIC) School of Life Sciences Peking UniversityBeijing 100871 China

BACKGROUND： Visualization of chromosomal loci location and dynamics is crucial for understanding many fundamental intra-nuclear processes such as DNA transcription, replication, and repair. OBJECTIVE： Here, we will describe the development of fluorescence labeling methods for chromatin imaging, including traditional as well as emerging chromatin labeling techniques in both fixed and live cells. We will also discuss current issues and provide a perspective on future developments and applications of the chromatin labeling technology. METHODS： A systematic literature search was performed using the PubMed. Studies published over the past 50 years were considered for review. More than 100 articles were cited in this review. RESULTS： Taking into account sensitivity, specificity, and spatiotemporal resolution, fluorescence labeling and imaging has been the most prevalent approach for chromatin visualization. Among all the fluorescent labeling tools, the adoption ofgenome editing tools, such as tale and CRISPR, have great potential for the labeling and imaging of chromatin. CONCLUSION： Although a number of chromatin labeling techniques are available for both fixed and live cells, much more effort is still clearly required to develop fluorescence labeling methods capable of targeting arbitrary sequences non-intrusively to allow long-term, multiplexing, and high-throughput imaging of genomic loci and chromatin structures. The emerging technological advances will outline a next-generation effort toward the comprehensive delineation of chromatin at single-cell level with single-molecule resolution.

关键词： chromatin structure and dynamics FROS FISH tale CRISPR/Cas9 single-guide RNA Suntag,super-resolution imaging

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水稻白叶枯病抗病机制与抗病育种展望

植物生理学报 2020年第12期56卷 2533-2542页

作者：陈复旦颜丙霄何祖华浙江大学杭州310058 中国科学院大学北京100049 中国科学院分子植物科学卓越创新中心植物分子遗传国家重点实验室上海200032

水稻白叶枯病抗病基因的发掘与育种利用是目前针对白叶枯病害(Xoo)最环保和有效的手段。迄今,已报道至少45个抗白叶枯病抗性基因(Xa),本文对已报道基因的发掘和定位进行了总结,并根据蛋白结构与抗病机制对其中已克隆的15个Xa基因进行了... 详细信息

水稻白叶枯病抗病基因的发掘与育种利用是目前针对白叶枯病害(Xoo)最环保和有效的手段。迄今,已报道至少45个抗白叶枯病抗性基因(Xa),本文对已报道基因的发掘和定位进行了总结,并根据蛋白结构与抗病机制对其中已克隆的15个Xa基因进行了分类,包括:(1)编码蛋白激酶受体的抗性基因,如RLK(receptor-like kinase);(2)编码NLR (nucleotide-binding leucine-rich repeat)免疫受体的抗性基因;(3)糖转运蛋白SWEET编码基因;(4)抗性执行基因(executor);(5)其他类型基因。Xa21作为第一个被克隆的白叶枯抗性基因,其编码蛋白RLK所介导的抗病机制已较为清晰。研究发现,除编码RLK的Xa基因外,大部分Xa发挥功能依赖于Xoo III型分泌系统分泌的类转录激活效应因子(transcriptionalactivator-likeeffectors,tale)。很多受白叶枯菌分泌的tale效应因子调控表达的水稻Xa基因实际为感病基因,其隐性突变产生抗病性。目前为止,白叶枯病non-tale效应因子与抗病蛋白互作尚无报道,因此,突破口将是对编码NLR的Xa基因的抗病机制的研究。本文结合Xa的来源、抗谱、机制的研究及当前抗病育种存在的问题,针对白叶枯病广谱抗病分子育种提出了展望。

关键词：白叶枯抗病基因抗病机制 RLK NLR SWEET tale

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Number One Love

The World of Chinese 2019年第4期 82-84页

作者： TINA XU 不详

Every life can be defined by two great love stories, says philosopher Alain de Botton. The first, the quest for sexual love, is the staple of music and literature."The second-the story of our quest for love from the world-is a more secret and shameful tale, yet this second love story is no less intense than the first. There is heartbreak here too."

关键词： Number One Love shameful tale heartbreak

Loss-of-susceptibility enables rice resistance to bacterial leaf blight and bacterial Leaf Streak

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Loss-of-susceptibility enables rice resistance to bacterial ...