检索结果-南通市图书馆

A 5′trna-Ala-derived small RNA regulates anti-fungal defense in plants

Science China(Life Sciences) 2022年第1期65卷 1-15页

作者： Hanqing Gu Bi Lian Yuxiang Yuan Ci Kong Yan Li Chang Liu Yijun Qi Center for Plant Biology School of Life SciencesTsinghua UniversityBeijing 100084China Tsinghua University-Peking University Joint Center for Life Sciences Tsinghua UniversityBeijing 100084China

Apart from their primordial role in protein synthesis,t rnas can be cleaved to produce t RNA-derived small rnas(ts rnas).The biological functions of ts rnas in plants remain largely unknown.In this study,we developed Rtc B ligation-based small RNA(s RNA)sequencing,a method that captures and distinguishes between 3′-2′,3′-cyclic-phosphate(c P)/phosphate(P)-terminated s rnas and 3′-OH-terminated s rnas,and profiled 5′ts rnas and 5′t RNA halves in Arabidopsis thaliana.We found that Arabidopsis 5′ts rnas and 5′t RNA halves predominantly contain a c P at the 3′end and require S-like rnase 1(RNS1)and RNS3 for their production.One of the most abundant 5′ts rnas,5′ts R-Ala,by associating with AGO1,negatively regulates Cytochrome P45071 A13(CYP71 A13)expression and camalexin biosynthesis to repress anti-fungal defense.Interestingly,5′ts R-Ala is downregulated upon fungal infection.Our study provides a global view of 5′ts rnas and 5′t RNA halves in Arabidopsis and unravels an important role of a 5′ts RNA in regulating anti-fungal defense.

关键词： trna-derived small rnas small RNA sequencing rnase T2 CYP71A13 disease resistance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Development of a trna-derived small RNA diagnostic and prognostic signature in liver cancer

引用

Genes & Diseases 2022年第2期9卷 393-400页

作者： Yi Zuo Shaoqiu Chen Lingling Yan Ling Hu Scott Bowler Emory Zitello Gang Huang Youping Deng Tianyou Hospital Affiliated to Wuhan University of Science and TechnologyWuhan 430064PR China Department of Quantitative Health Sciences John A.Burns School of MedicineUniversity of Hawaii at ManoaHonoluluHI 96813USA Molecular Biosciences and Bioengineering Program College of Tropical Agriculture and Human ResourcesUniversity of Hawaii at ManoaHonoluluHI 96822USA Shanghai Key Laboratory for Molecular Imaging Shanghai University of Medicine and Health SciencesShanghai 201318PR China

Liver cancer presents divergent clinical behaviors.There remain opportunities for molecular markers to improve liver cancer diagnosis and prognosis,especially since trna-derived small rnas(tsRNA)have rarely been studied.In this study,a random forests(RF)diagnostic model was built based upon tsRNA profiling of paired tumor and adjacent normal samples and validated by independent validation(IV).A LASSO model was used to developed a seven-tsRNA-based risk score signature for liver cancer prognosis.Model performance was evaluated by a receiver operating characteristic curve(ROC curve)and Precision-Recall curve(PR curve).The five-tsRNA-based RF diagnosis model had area under the receiver operating characteristic curve(AUROC)88%and area under the precision–recall curve(AUPR)87%in the discovery cohort and 87%and 86%in IV-AUROC and IV-AUPR,respectively.The seven-tsRNA-based prognostic model predicts the overall survival of liver cancer patients(Hazard Ratio 2.02,95%CI 1.36–3.00,P<0.001),independent of standard clinicopathological prognostic factors.Moreover,the model successfully categorizes patients into high-low risk groups.Diagnostic and prognostic modeling can be reliably utilized in the diagnosis of liver cancer and high-low risk classification of patients based upon tsRNA characterization.

关键词： Diagnosis Liver cancer Prognosis Random forests trna-derived small rnas

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

5′-tRF-GlyGCC: a trna-derived small RNA as a novel biomarker for colorectal cancer diagnosis

5′-tRF-GlyGCC: a tRNA-derived small RNA as a novel biomarke...

引用

2021年中国肿瘤标志物学术大会暨第十五届肿瘤标志物青...

作者： Jiexin Li Sun Yat-sen University

Background: trna-derived small rnas（tD Rs）, which are widely distributed in human tissues including blood and urine, play an important role in the progression of cancer. However, the expression of tD Rs in colorectal cancer（CRC） plasma and their potential diagnostic values have not been systematically explored.Methods: The expression profiles of tD Rs in plasma of CRC and health controls（HCs） are investigated by small RNA sequencing. The level and diagnostic value of 5′-tRF-GlyGCC are evaluated by quantitative PCR in plasma samples from 105 CRC patients and 90 HCs. The mechanisms responsible for biogenesis of 5′-tRF-GlyGCC are checked by in vitro and in vivo models.Results: 5′-tRF-GlyGCC is dramatically increased in plasma of CRC patients compared to that of HCs.The area under curve（AUC） for 5′-tRF-GlyGCC in CRC group is 0.882. The combination of carcinoembryonic antigen（CEA） and carbohydrate antigen 199（CA199） with 5′-tRF-GlyGCC improves the AUC to 0.926. Consistently, the expression levels of 5′-tRF-GlyGCC in CRC cells and xenograft tissues are significantly greater than that in their corresponding controls. Blood cells cocultured with CRC cells or mice xenografted with CRC tumors show increased levels of 5′-tR FGlyGCC. In addition, we find that the increased expression of 5′-tRF-GlyGCC is dependent on the upregulation of AlkB homolog 3（ALKBH3）, a trna demethylase which can promote trna cleaving to generate tD Rs.Conclusions: The level of 5′-tRF-GlyGCC in plasma is a promising diagnostic biomarker for CRC diagnosis.

关键词： 5′-tRF-GlyGCC CRC ALKBH3 Plasma trna-derived small rnas

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：