A 5′tRNA-Ala-derived small RNA regulates anti-fungal defense in plants
A 5′ tRNA-Ala-derived small RNA regulates anti-fungal defense in plants作者机构:Center for Plant BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China Tsinghua University-Peking University Joint Center for Life SciencesTsinghua UniversityBeijing 100084China
出 版 物:《Science China(Life Sciences)》 (中国科学(生命科学英文版))
年 卷 期:2022年第65卷第1期
页 面:1-15页
核心收录:
学科分类:0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种]
基 金:supported by the National Natural Science Foundation of China(31801074 31788103)
主 题:tRNA-derived small RNAs small RNA sequencing RNase T2 CYP71A13 disease resistance
摘 要:Apart from their primordial role in protein synthesis,t RNAs can be cleaved to produce t RNA-derived small RNAs(ts RNAs).The biological functions of ts RNAs in plants remain largely *** this study,we developed Rtc B ligation-based small RNA(s RNA)sequencing,a method that captures and distinguishes between 3′-2′,3′-cyclic-phosphate(c P)/phosphate(P)-terminated s RNAs and 3′-OH-terminated s RNAs,and profiled 5′ts RNAs and 5′t RNA halves in Arabidopsis *** found that Arabidopsis 5′ts RNAs and 5′t RNA halves predominantly contain a c P at the 3′end and require S-like RNase 1(RNS1)and RNS3 for their *** of the most abundant 5′ts RNAs,5′ts R-Ala,by associating with AGO1,negatively regulates Cytochrome P45071 A13(CYP71 A13)expression and camalexin biosynthesis to repress anti-fungal ***,5′ts R-Ala is downregulated upon fungal *** study provides a global view of 5′ts RNAs and 5′t RNA halves in Arabidopsis and unravels an important role of a 5′ts RNA in regulating anti-fungal defense.