A 5′tRNA-Ala-derived small RNA regulates anti-fungal defense in plants

作     者：Hanqing Gu Bi Lian Yuxiang Yuan Ci Kong Yan Li Chang Liu Yijun Qi Hanqing Gu;Bi Lian;Yuxiang Yuan;Ci Kong;Yan Li;Chang Liu;Yijun Qi

作者机构：Center for Plant BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China Tsinghua University-Peking University Joint Center for Life SciencesTsinghua UniversityBeijing 100084China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2022年第65卷第1期

页      面：1-15页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：supported by the National Natural Science Foundation of China(31801074 31788103) 

主　　题：tRNA-derived small RNAs small RNA sequencing RNase T2 CYP71A13 disease resistance 

摘      要：Apart from their primordial role in protein synthesis,t RNAs can be cleaved to produce t RNA-derived small RNAs(ts RNAs).The biological functions of ts RNAs in plants remain largely *** this study,we developed Rtc B ligation-based small RNA(s RNA)sequencing,a method that captures and distinguishes between 3′-2′,3′-cyclic-phosphate(c P)/phosphate(P)-terminated s RNAs and 3′-OH-terminated s RNAs,and profiled 5′ts RNAs and 5′t RNA halves in Arabidopsis *** found that Arabidopsis 5′ts RNAs and 5′t RNA halves predominantly contain a c P at the 3′end and require S-like RNase 1(RNS1)and RNS3 for their *** of the most abundant 5′ts RNAs,5′ts R-Ala,by associating with AGO1,negatively regulates Cytochrome P45071 A13(CYP71 A13)expression and camalexin biosynthesis to repress anti-fungal ***,5′ts R-Ala is downregulated upon fungal *** study provides a global view of 5′ts RNAs and 5′t RNA halves in Arabidopsis and unravels an important role of a 5′ts RNA in regulating anti-fungal defense.