检索结果-南通市图书馆

Fundamental Research 2023年第3期3卷 459-468页

作者： Wenli Wang Dingqiang Lu Qiuda Xu Yulian Jin Guangchang Pang Yuan Liu Department of Food Science&Technology School of Agriculture&BiologyShanghai Jiao Tong UniversityShanghai 200240China College of Biotechnology&Food Science Tianjin University of CommerceTianjin 300134China

sweet and umami tastes are elicited by sweet and umami receptors on the tongue and palate epithelium,respectively.However,the molecular machinery allowing the taste reaction remains incompletely understood.Through a phosphoproteomic approach,we identified the key proteins that trigger taste mechanisms based on phosphorylation cascades.Ryanodine receptor isoform 1(RYR1)was further verified by sensory and behavioral assays.We propose a model of RYR1-mediated sweet/umami signaling in which the RYR1 channel,which mediates Ca^(2+)release from the endoplasmic reticulum,is closed by dephosphorylation in bud tissue after sweet/umami treatment.The alteration in Ca^(2+)content in the cytosol induces transient membrane depolarization and generates a cell current for taste signal transduction.We demonstrate that RYR1 is a new channel involved in the regulation of sweet/umami signal transduction and propose a“metabolic clock”notion based on sweet/umami sensing.Our study provides a valuable foundation for a system-level understanding of the taste perception mechanism.

关键词： Umami sweet Phosphoproteomic Ryanodine receptor isoform 1 Metabolic clock

Simultaneous changes in seed size, oil content and protein content driven by selection of sweet homologues during soybean domestication

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

National Science Review 2020年第11期7卷 1776-1786页

作者： Shoudong Wang Shulin Liu Jie Wang Kengo Yokosho Bin Zhou Ya-Chi Yu Zhi Liu Wolf B.Frommer Jian Feng Ma Li-Qing Chen Yuefeng Guan Huixia Shou Zhixi Tian State Key Laboratory of Plant Physiology and Biochemistry College of Life sciences Zhejiang University State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Innovation Academy for Seed Design Chinese Academy of Sciences University of Chinese Academy of Sciences College of Resources and Environment Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology Fujian Agriculture and Forestry University Institute of Plant Science and Resources Okayama University Institute of Crop Science Anhui Academy of Agricultural Sciences Department of Plant Biology School of Integrative Biology University of Illinois at Urbana-Champaign Institute for Molecular Physiology and Cluster of Excellence on Plant Sciences (CEPLAS) Heinrich Heine University of Düsseldorf FAFU-UCR Joint Center for Horticultural Plant Biology and Metabolomics Haixia Institute of Science and Technology Fujian Agriculture and Forestry University

Soybean accounts for more than half of the global production of oilseed and more than a quarter of the protein used globally for human food and animal feed. Soybean domestication involved parallel increases in seed size and oil content, and a concomitant decrease in protein content. However, science has not yet discovered whether these effects were due to selective pressure on a single gene or multiple genes. Here,re-sequencing data from>800 genotypes revealed a strong selection during soybean domestication on Gm sweet10a. The selection of Gm sweet10a conferred simultaneous increases in soybean-seed size and oil content as well as a reduction in the protein content. The result was validated using both near-isogenic lines carrying substitution of haplotype chromosomal segments and transgenic soybeans. Moreover,Gm sweet10b was found to be functionally redundant with its homologue Gm sweet10a and to be undergoing selection in current breeding, leading the the elite allele Gm sweet10b, a potential target for present-day soybean breeding. Both Gmsweet10a and Gmsweet10b were shown to transport sucrose and hexose, contributing to sugar allocation from seed coat to embryo, which consequently determines oil and protein contents and seed size in soybean. We conclude that past selection of optimal Gm sweet10a alleles drove the initial domestication of multiple soybean-seed traits and that targeted selection of the elite allele Gm sweet10b may further improve the yield and seed quality of modern soybean cultivars.

关键词： soybean domestication yield seed quality sweet

Rice Transcription Factor OsDOFll Modulates Sugar Transport by Promoting Expression of Sucrose Transporter and sweet Genes

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2018年第6期11卷 833-845页

作者： Yunfei Wu Sang-Kyu Lee Youngchul Yoo Jinhuan Wei Suk-Yoon Kwon Sang-Won Lee Jong-Seong Jeon Gynheung An Crop Biotech Institute and Graduate School of Biotechnology Kyung Hee University Yongin 17104 Korea Plant Systems Engineering Research Center Korea Research Institute of Bioscience and Biotechnology Daejeon 34141 Korea

Sucrose is produced in mesophyll cells and transferred into phloem cells before it is delivered long- distance to sink tissues. However, little is known about how sucrose transport is regulated in plants. Here, we identified a T-DNA insertional mutant of Oryza sativa DNA BINDING WITH ONE FINGER 11 （OsDOF11）, which is expressed in the vascular cells of photosynthetic organs and in various sink tissues. The osdofll mutant plants are semi-dwarf and have fewer tillers and smaller panicles as compared with wild-type （WT） plants. Although sucrose enhanced root elongation in young WT seedlings, this enhance- ment did not occur in osdof11 seedlings due to reduced sucrose uptake. Sugar transport rate analyses revealed that less sugar was transported in osdofll plants than in the WT. Expression of four Sucrose Transporter （SUT） genes-OsSUT1, OsSUT3, OsSUT4, and OsSUT5-as well as two Sugars Will Eventually be Exported Transporters （sweet） genes, Ossweetll and Ossweet14, was altered in various organs of the mutant, including the leaves. Chromatin immunoprecipitation assays showed that OsDOFll directly binds the promoter regions of SUT1, Ossweetll, and Ossweet14, indicating that the expression of these transporters responsible for sucrose transport via apopiastic loading is coordinately controlled by OsDOFll. We also observed that osdofll mutant plants were less susceptible to infection byXanthomonas oryzae pathovar oryzae, suggesting that OsDOFll participates in sugar distribution during pathogenic in- vasion. Collectively, these results suggest that OsDOFll modulates sugar transport by regulating the expression of both SUT and sweet genes in rice.

关键词： OsDOF11 phloem loading rice SUT sweet Xanthomonas oryzae

油茶sweet基因家族的全基因组鉴定及表达分析

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

生物技术通报 2024年第5期40卷 179-190页

作者：杜兵帅邹昕蕙王子豪张馨元曹一博张凌云林木资源高效生产全国重点实验室森林培育与保护教育部重点实验室北京林业大学林学院北京100083

【目的】挖掘参与油茶糖代谢及逆境响应的糖外排转运子(sugars will eventually be exported transporters,sweets)。【方法】利用生物信息学方法分析油茶sweets家族的基因结构、蛋白基序、染色体定位、共线性关系、启动子区顺式作用元... 详细信息

【目的】挖掘参与油茶糖代谢及逆境响应的糖外排转运子(sugars will eventually be exported transporters,sweets)。【方法】利用生物信息学方法分析油茶sweets家族的基因结构、蛋白基序、染色体定位、共线性关系、启动子区顺式作用元件及上游调控因子等,并利用RT-qPCR分析Cosweets在不同时期、不同组织及不同逆境胁迫下的基因表达情况。【结果】从油茶中鉴定得到14个Cosweets基因,不均匀分布于10条染色体上,不同成员间内含子-外显子数目存在差异。根据系统进化关系,14个Cosweets可分为 4个分支,均具有1-2个MtN3 保守结构域,同一分支具有相似的基因结构和基序。根据启动子顺式作用元件和上游转录因子预测的分析结果,Cosweets启动子中含有多个与生长发育、植物激素和应激相关的调节元件,其表达可能受到ERF、DOF、BBR-BPC、MYB等转录因子的调控。RT-qPCR分析表明大部分Cosweets成员在果实和根中高表达,在种子中的表达水平与发育时期相关,并根据低温、高盐和干旱等非生物胁迫下Cosweets的表达模式挖掘出Cosweet1、Cosweet2、Cosweet17等响应油茶低温、干旱或高盐胁迫的基因。【结论】Cosweet基因的表达受到多种激素及转录因子调控,并在油茶种子发育与逆境胁迫响应中发挥重要作用。

关键词：油茶 sweet 蛋白结构表达模式分析非生物胁迫

中国水仙sweet基因在开花及鳞茎膨大过程的表达分析

在线全文

学校读者我要写书评

暂无评论

分子植物育种 2024年第7期22卷 2119-2126页

作者：徐静魏培宁杨静文吕美玲伍炳华袁媛福建农林大学园艺学院福州350002 福建农林大学生命科学学院福州350002

sweet转运蛋白通过交替转运机制介导单糖和二糖双向跨膜运输,在植物的生长发育过程中糖的分配和利用起关键作用。本研究基于水仙(Narcissus tazetta var. chinensis)转录组分析获得的9条在花器官中表达量相对较高的9个SWEEET基因,这些Nt... 详细信息

sweet转运蛋白通过交替转运机制介导单糖和二糖双向跨膜运输,在植物的生长发育过程中糖的分配和利用起关键作用。本研究基于水仙(Narcissus tazetta var. chinensis)转录组分析获得的9条在花器官中表达量相对较高的9个SWEEET基因,这些Ntsweets在sweet家族的4个分支中皆有分布。在水仙开花过程中,Ntsweet1a、Ntsweet1b和Ntsweet4b基因在花被片中的表达水平随着花朵的开放表达量逐渐上升,其他基因则无明显规律;在花芽分化过程中,Ntsweet1a、Ntsweet1b和Ntsweet13既有较高的表达水平,又在鳞茎中各组织里的表达水平随着花芽分化而逐渐上升;在水仙鳞茎膨大期间,Ntsweet1a在鳞茎膨大期间保持较高的表达量,且随着鳞茎的膨大表达水平上升。进一步利用酵母功能互补实验分析这些sweet蛋白对葡萄糖、半乳糖、甘露糖、果糖、蔗糖的转运功能,发现Ntsweet1a可以转运葡萄糖和半乳糖,暗示Ntsweet1a在开花、花芽分化及鳞茎膨大过程中参与了糖的转运,特别是葡萄糖和半乳糖的转运有重要作用。

关键词：中国水仙 sweet 转运蛋白开花鳞茎生长

Impaired sweet-mediated sugar transportation impacts starch metabolism in developing rice seeds

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2022年第1期10卷 98-108页

作者： Pei Li Lihan Wang Hongbo Liu Meng Yuan National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWuhan 430070HubeiChina

Sugar transportation and sugar-to-starch metabolism are considered important processes in seed development and embryo viability.A few plant sweet proteins acting as sugar transporters have been reported to function in inflorescence and/or seed development.Here,we identified seven members of the 21 Os sweet genes in rice that play essential roles in sugar transportation and sugar-to-starch conversion in seed development.Nineteen Os sweet genes exhibiting different expression patterns during inflorescence and seed development were knocked out individually by CRISPR/Cas9.One third of the mutants showed decreased fertile pollen viability and shriveled mature caryopses,resulting in weakened seed traits.Grain fill-related genes but not representative grain shape-regulating genes showed attenuated expression in the mutants.Seed of each of these mutants accumulated more sucrose,glucose or fructose but less starch.Among all Os sweet genes,Os sweet4 and Os sweet11 had major effects on caryopsis development.The sugar-to-starch metabolic pathway was significantly altered in ossweet11 mutants based on differential expression analysis in RNA sequencing assays,confirming that Os sweet11 functions as a sugar transporter with a key role in seed development.These results help to decipher the multiple functions of Os sweet genes and to show how they might be used in genetic improvement of rice.

关键词： Oryza sativa Sugar transporter sweet Sugar Starch

花椰菜糖转运基因sweet的克隆和功能鉴定

在线全文

学校读者我要写书评

暂无评论

分子植物育种 2023年

作者：文正华张伟单晓政江汉民孙德岭张小丽天津科润农业科技股份有限公司蔬菜研究所天津市蔬菜遗传育种企业重点实验室天津市农业科学院蔬菜生物育种全国重点实验室

sweet是一种介导葡萄糖、蔗糖等从韧皮部薄壁细胞向质外体运输的糖转运体，在植物抵御生物胁迫中发挥重要作用。本研究从花椰菜C-8基因组中鉴定出30个sweet基因，其中28个基因编码蛋白包含2个MtN3＿slv结构域和1个跨膜结构区域。利用转... 详细信息

sweet是一种介导葡萄糖、蔗糖等从韧皮部薄壁细胞向质外体运输的糖转运体，在植物抵御生物胁迫中发挥重要作用。本研究从花椰菜C-8基因组中鉴定出30个sweet基因，其中28个基因编码蛋白包含2个MtN3＿slv结构域和1个跨膜结构区域。利用转录组数据分析了sweet基因在根肿菌不同侵染时期、抗感材料中的表达量差异，结果显示绝大多数基因在感病材料CS45 T1或T2时期的表达量明显高于抗病材料CR12。利用CRISPR-Cas9技术对花椰菜BOBsweet11和BOBsweet12基因进行定向编辑，共有14株T0代转基因阳性株系发生了编辑事件，其中3个植株2个基因均发生了编辑；进一步筛选出T1-#11和T1-#14纯合双编辑植株，并对其T2代株系sweet11;12-#11和sweet11;12-#14进行根肿病抗性鉴定，结果显示，与野生型相比，纯合双编辑单株均表现出相对较强的根肿病抗性。本研究表明，通过CRISPR-Cas9技术对花椰菜糖转运基因sweet11和sweet12进行编辑可创制抗根肿病新种质。

关键词：花椰菜 sweet 根肿病 CRISPR-Cas9基因编辑功能分析

植物 sweet 基因家族促进病害发生的研究进展

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

江苏农业学报 2022年第5期38卷 1411-1420页

作者：沈川李夏安康学院陕南生态经济研究中心陕西安康725000 安康市农业科学研究院陕西安康725000

糖的运输和分配在调节植物的生长发育和应对生物和非生物胁迫中起着关键作用。在植物-病原菌相互作用过程中,存在着对糖的竞争,这种竞争由膜运输体控制且对植物和病原互作的结果具有决定性作用。sweet糖转运蛋白是细胞外病原菌的靶向目... 详细信息

糖的运输和分配在调节植物的生长发育和应对生物和非生物胁迫中起着关键作用。在植物-病原菌相互作用过程中,存在着对糖的竞争,这种竞争由膜运输体控制且对植物和病原互作的结果具有决定性作用。sweet糖转运蛋白是细胞外病原菌的靶向目标,病原菌通过修改其表达水平以获得生长所需的糖分营养。本文阐述了植物中sweet家族的分布和结构,归纳总结了sweet在细菌、真菌和卵菌入侵寄主植物过程中所扮演的角色,最后展望了通过基因工程手段操纵sweet基因的表达对于开发抗病栽培品种的前景。

关键词： sweet 糖转运蛋白植物-微生物互作基因工程生物胁迫

Chromosome-scale genome assembly of sweet cherry(Prunus avium L.)cv.Tieton obtained using long-read and Hi-C sequencing

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2020年第1期7卷 1214-1224页

作者： Jiawei Wang Weizhen Liu Dongzi Zhu Po Hong Shizhong Zhang Shijun Xiao Yue Tan Xin Chen Li Xu Xiaojuan Zong Lisi Zhang Hairong Wei Xiaohui Yuan Qingzhong Liu Shandong Key Laboratory of Fruit Biotechnology Breeding Shandong Institute of PomologyTaianShandong 271000China School of Computer Science and Technology Wuhan University of TechnologyWuhanHubei 430070China State Key Laboratory of Crop Biology Shandong Agricultural UniversityTaianShandong 271018China Gooal Gene WuhanHubei 430070China

sweet cherry(Prunus avium)is an economically significant fruit species in the genus Prunus.However,in contrast to other important fruit trees in this genus,only one draft genome assembly is available for sweet cherry,which was assembled using only Illumina short-read sequences.The incompleteness and low quality of the current sweet cherry draft genome limit its use in genetic and genomic studies.A high-quality chromosome-scale sweet cherry reference genome assembly is therefore needed.A total of 65.05 Gb of Oxford Nanopore long reads and 46.24 Gb of Illumina short reads were generated,representing~190x and 136x coverage,respectively,of the sweet cherry genome.The final de novo assembly resulted in a phased haplotype assembly of 344.29 Mb with a contig N50 of 3.25 Mb.Hi-C scaffolding of the genome resulted in eight pseudochromosomes containing 99.59%of the bases in the assembled genome.Genome annotation revealed that more than half of the genome(59.40%)was composed of repetitive sequences,and 40,338 protein-coding genes were predicted,75.40%of which were functionally annotated.With the chromosomescale assembly,we revealed that gene duplication events contributed to the expansion of gene families for salicylic acid/jasmonic acid carboxyl methyltransferase and ankyrin repeat-containing proteins in the genome of sweet cherry.Four auxin-responsive genes(two GH3s and two SAURs)were induced in the late stage of fruit development,indicating that auxin is crucial for the sweet cherry ripening process.In addition,772 resistance genes were identified and functionally predicted in the sweet cherry genome.The high-quality genome assembly of sweet cherry obtained in this study will provide valuable genomic resources for sweet cherry improvement and molecular breeding.

关键词： sweet bases assembly

Gibberellin induced shot berry formation in cv.Early sweet is a direct consequence of high fruit set

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2020年第1期7卷 733-744页

作者： Etti Or Orly Oren Tamar Halaly-Basha Padmalatha Koilkonda Zhaowan Shi Chuanlin Zheng Atiako Kwame Acheampong Institute of Plant Sciences Department of Fruit Tree SciencesAgricultural Research OrganizationVolcani CenterRishon LeZion 7528809Israel Division of Crop Sciences ICAR-Central Research Institute for Dryland AgricultureSantoshnagarHyderabadTelangana 500059India Department of Fruit Tree Sciences College of HorticultureChina Agricultural UniversityBeijing 100193China Biology department University of North CarolinaChapel HillNC 27514USA

The‘seedless’table grape industry relies mainly on stenospermocarpic cultivars,in which endosperm abortion results in berries with seed rudiments and low levels of bioactive gibberellin(GA).Application of GA to enhance berry sizing in these cultivars is often accompanied by adverse effects,one of which is increased proportions of very small berries(termed shot berries).Manual removal of these berries,which is essential to improve uniformity and market value,increases production cost and exposes the cluster to damage.Unraveling the physiological causes of shot berry formation is thus of both scientific and practical value.This study focuses on understanding the GA-mediated regulation of shot berry formation in Vitis vinifera cv.Early sweet,known for a high proportion of shot berries,which severely damage cluster appearance.As GA is known to induce the parthenocarpic fruit set,we first tested the assumption that the parthenocarpic nature of a fruitlet is a primary cause for shot berry development.We then examined the consequence of the flower load on the proportion of shot berries in the cluster.Our data suggests that:(1)contrary to prior assumptions,the parthenocarpic nature of a fruitlet is not the primary cause for shot berry development,demonstrated by the fact that parthenocarpic fruitlets develop into a full-size berries;(2)the proportion of shot berries on a cluster is a function of the initial flower load on the inflorescence,with high initial flower load resulting in greater shot berry percentage in the cluster;(3)GA treatment bypasses the natural regulation of flower load,resulting in high fruitlet density and increased competition among fruitlets;(4)variation of flower load within the cluster influences berry size uniformity to a greater extent than does the variation in number of cluster per vine.The identity of the factors that determine the fate of a given flower on a high-load cluster remains an open question.

关键词： sweet consequence cluster