Impaired SWEET-mediated sugar transportation impacts starch metabolism in developing rice seeds

作     者：Pei Li Lihan Wang Hongbo Liu Meng Yuan Pei Li;Lihan Wang;Hongbo Liu;Meng Yuan

作者机构：National Key Laboratory of Crop Genetic ImprovementNational Center of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWuhan 430070HubeiChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2022年第10卷第1期

页      面：98-108页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 0902[农学-园艺学] 

基　　金：supported by the National Natural Science Foundation of China(31821005,31822042,and 31871946) the Natural Science Foundation of Hubei Province(2020CFA058) the Fundamental Research Funds for the Central Universities(2662019FW006)。 

主　　题：Oryza sativa Sugar transporter SWEET Sugar Starch 

摘      要：Sugar transportation and sugar-to-starch metabolism are considered important processes in seed development and embryo viability.A few plant SWEET proteins acting as sugar transporters have been reported to function in inflorescence and/or seed development.Here,we identified seven members of the 21 Os SWEET genes in rice that play essential roles in sugar transportation and sugar-to-starch conversion in seed development.Nineteen Os SWEET genes exhibiting different expression patterns during inflorescence and seed development were knocked out individually by CRISPR/Cas9.One third of the mutants showed decreased fertile pollen viability and shriveled mature caryopses,resulting in weakened seed traits.Grain fill-related genes but not representative grain shape-regulating genes showed attenuated expression in the mutants.Seed of each of these mutants accumulated more sucrose,glucose or fructose but less starch.Among all Os SWEET genes,Os SWEET4 and Os SWEET11 had major effects on caryopsis development.The sugar-to-starch metabolic pathway was significantly altered in ossweet11 mutants based on differential expression analysis in RNA sequencing assays,confirming that Os SWEET11 functions as a sugar transporter with a key role in seed development.These results help to decipher the multiple functions of Os SWEET genes and to show how they might be used in genetic improvement of rice.