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Alteration of tumor suppressor gene p16 and Rb in gastric cancinogesis

World Journal of Gastroenterology 1997年第4期3卷 64-64页

作者： ZHOU Qi1, ZOU JianXiang2, CHEN YuLong2, YU HuiZhen3,WANG LiDong1, LI YongXin1, GUO HuaQin1, GAO ShanShan1, and QIU SongLian11Laboratory for Cancer Research, Medical Experimental Center, 2Department of Gas

IM To study the alterations of tumor suppressor gene p16 and Rb in the carcinogenesis of the stomach. METHODS Different mucosal biopsies were endoscopically obtained, all samples were immediately fixed with 10% buffered formalin, embedded with paraffin and sectioned serielly. Alterations of p16 and Rb protein in 12 cases of superficial gastritis, 15 atrophic gastritis, 20 atypical hyperplasia and 40 cancerous tissues were detected by the immunohistochemical method (ABC). RESULTS Different degrees of nuclear immunostaining of p16 and Rb occurred on gastric epithelium in different stages of lesions. With the lesions progressing, the positive immunostaining rate of p16 protein had a decreasing tendency (833%→733%→300%→275%), and on the other hand, that of Rb protein had an increasing tendency (250%→467%→600%→675%). A negative correlationship was found between these two parameters in the gastric cancer. Of 40 cases of gastric cancer, a negative relationship was observed in 20 cases. In comparison with both positive (9 cases) and both negative tissues (11 cases), there was a significant difference (500%,225%,275%) (P<005).CONCLUSION Abnormal expression of p16 and Rb plays an important role in gastric carcinogenesis.

关键词： genes, suppressor, tumor gene expression retinoblastoma protein/metabolism stomach neoplasms/metabolism carcinoma/metabolism

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Changes　of　p53　and　Waf1p21　and　cell　proliferation　in　esophageal　carcinogenesis

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World Journal of Gastroenterology 1997年第2期3卷 30-32页

作者： WANG Li Dong 1, YANG Wan Cai 1, ZHOU Qi 1, XING Ying 1,JIA Yun Ying 2 and ZHAO Xin 1

AIM To study the correlationship between the changes of p53, Waf1p21 and the cell proliferation determined by PCNA at different stages of human esophageal carcinogenesis. METHODS Biopsied and resected esophageal tissues from a high risk population for esophageal cancer in northern China were used in this study. All the specimens were fixed with 85% alcohol and further processed with routine histology. The avidin biotin peroxidase complex (ABC) method was used for the detection of p53, Waf1p21 and PCNA. RESULTS The strong nuclear staining for p53, Waf1p21 and PCNA was observed in the normal esophageal epithelium and the epithelia with different severities of lesions. As the lesions progressed to dysplasia (DYS) and to esophageal squamons cell carcinoma (SCC), the percentage of Waf1p21 immunoreactivity decreased. The number of Waf1p21 immunostaining positive cells increased slightly from normal to basal cell hyperplasia (BCH), but there was no further increase in DYS and in SCC. The total number of positive cells for Waf1p21 stain appeared to be lower than that of p53 in normal and BCH esophageal epithelia and much lower in DYS and SCC. The Waf1p21 positive immunostaining cells were located at the third and forth cell layers in half of the samples examined, which was 2～4 cell layers higher than that of PCNA and p53 in the same histological categories of normal, BCH and DYS. CLNCLUSION The low levels of Waf1p21 at the stage of DYS may be related to a functional loss of p53. Other mechanisms may also be responsible to the lack of Waf1p21 expression in DYS and SCC.

关键词： esophageal neoplasms precancerous conditions p53 genes Waf1p21 genes, suppressor, tumor

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A preliminary study on the loss of heterozygosity at 17p13 in gastric and colorectal cancers

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World Journal of Gastroenterology 1997年第3期3卷 32-34页

作者： WU Guo Jun 1, SHAN Xiang Nian 2, LI Ming Fa 2, SHI Shao Lin 1, ZHENG Qi Ping 1, YU Long 1 and ZHAO Shou Yuan 1

Ａｐｒｅｌｉｍｉｎａｒｙｓｔｕｄｙｏｎｔｈｅｌｏｓｓｏｆｈｅｔｅｒｏｚｙｇｏｓｉｔｙａｔ１７ｐ１３ｉｎｇａｓｔｒｉｃａｎｄｃｏｌｏｒｅｃｔａｌｃａｎｃｅｒｓＷＵＧｕｏＪｕｎ１，ＳＨＡＮＸｉａｎｇＮｉａｎ２，ＬＩＭ...

Ａｐｒｅｌｉｍｉｎａｒｙｓｔｕｄｙｏｎｔｈｅｌｏｓｓｏｆｈｅｔｅｒｏｚｙｇｏｓｉｔｙａｔ１７ｐ１３ｉｎｇａｓｔｒｉｃａｎｄｃｏｌｏｒｅｃｔａｌｃａｎｃｅｒｓＷＵＧｕｏＪｕｎ１，ＳＨＡＮＸｉａｎｇＮｉａｎ２，ＬＩＭｉｎｇＦａ２，ＳＨＩＳｈａｏＬ...

关键词： stomach neoplasms colorectal neoplasms p53 gene heterozygosity loss genes, suppressor, tumor

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