检索结果-南通市图书馆

Analysis of Gene Expression Profile Induced by Water Stress in Upland Rice(Oryza sativa ***109)Seedlings using Subtractive Expressed Sequence Tags library

引用

Journal of Integrative Plant Biology 2007年第10期49卷 1455-1463页

作者： Haiguang Wang Hongliang Zhang Zichao Li Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture Key Laboratory of Crop Heterosis and Utilizition of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement China Agricultural University Beijing China100094

To identify the water stress induced genes of upland rice cultivar IRAT109, which is resistant to drought, a subtractive cDNA library was developed from polyethylene glycol- （PEG） treated and non-treated seedlings by suppression subtractive hybridization, from which 2112 recombinant colonies were obtained, Eight hundred clones were selected randomly for sequencing analysis, and 384 unique expressed sequence tags （ESTs） were obtained, They were found to be involved in diverse biological processes, such as metabolism, transcription, signal transduction, protein synthesis and others, Notably a number of known functional genes in drought tolerance, including genes related to biosynthesis of osmoprotectants, defense against active oxygen, removal of toxic compounds, recovery of proteins and reinforcement of cell wall were also found in the study, Several genes related to deleterious responses were upregulated by PEG stress, The differential expression patterns of 11 SSH-derived ESTs were confirmed by real-time polymerase chain reaction.

关键词： expressed sequence tag subtracted library suppression subtractive hybridization upland rice water stress.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：