Analysis of Gene Expression Profile Induced by Water Stress in Upland Rice(Oryza sativa ***109)Seedlings using Subtractive Expressed Sequence Tags Library

作     者：Haiguang Wang Hongliang Zhang Zichao Li 

作者机构：Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture Key Laboratory of Crop Heterosis and Utilizition of Ministry of Education and Beijing Key Lab of Crop Genetic Improvement China Agricultural University Beijing China100094 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2007年第49卷第10期

页      面：1455-1463页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：Supported by the Specialized Research Fund for the Doctoral Program of Higher Education of the Ministry of Education of China(20030019004) the National Natural Science Foundation of China(30250009) 

主　　题：expressed sequence tag subtracted library suppression subtractive hybridization upland rice water stress. 

摘      要：To identify the water stress induced genes of upland rice cultivar IRAT109, which is resistant to drought, a subtractive cDNA library was developed from polyethylene glycol- （PEG） treated and non-treated seedlings by suppression subtractive hybridization, from which 2112 recombinant colonies were obtained, Eight hundred clones were selected randomly for sequencing analysis, and 384 unique expressed sequence tags （ESTs） were obtained, They were found to be involved in diverse biological processes, such as metabolism, transcription, signal transduction, protein synthesis and others, Notably a number of known functional genes in drought tolerance, including genes related to biosynthesis of osmoprotectants, defense against active oxygen, removal of toxic compounds, recovery of proteins and reinforcement of cell wall were also found in the study, Several genes related to deleterious responses were upregulated by PEG stress, The differential expression patterns of 11 SSH-derived ESTs were confirmed by real-time polymerase chain reaction.