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Arbuscular mycorrhiza and plant growth promoting endophytes facilitates accumulation of saponin under moderate drought stress

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Chinese Herbal Medicines 2024年第2期16卷 214-226页

作者： Shubiao Qian Qing Zhang Sha Li Rui Shi Xiahong He Shuhui Zi Tao Liu Yunnan Agricultural University Kunming 650201China National&Local Joint Engineering Research Center on Gemplasm Utilization&Innovation of Chinese Medicinal Materials in Southwest China Yunnan Agricultural UniversityKunming 650201China Institute of Sericulture and Honeybee Honghe 661101China Southwest Forestry University Kunming 650201China

Objective: Paris polyphylla var. yunnanensis, one of the important medicinal plant resources in Yunnan,China, usually takes 6–8 years to be harvested. Therefore, it is urgent to find a method that can not only shorten its growth years, but also improve its quality. In this study, we examined the effects of a combination treatment of arbuscular mycorrhizal fungi(AMF) and plant growth-promoting endophytes(PGPE)and drought stress on the accumulation of saponins in ***: P. polyphylla var. yunnanensis was infected with a mixture of AMF and PGPE under drought stress. The content of saponins, as well as morphological, physiological, and biochemical indicators, were all measured. The UGTs gene related to saponin synthesis was obtained from transcriptome data by homologous comparison, which were used for RT-PCR and phylogenetic ***: Regardless of water, AMF treatment could infect the roots of P. polyphylla var. yunnanensis, however double inoculation with AMF and PGPE(AMF + PGPE) would reduce the infection rate of AMF. Plant height, aboveground and underground fresh weight did not differ significantly between the single inoculation AMF and the double inoculation treatment under different water conditions, but the inoculation treatment significantly increased the plant height of P. polyphylla var. yunnanensis compared to the noninoculation treatment. Single inoculation with AMF considerably increased the net photosynthetic rate,stomatal conductance, and transpiration rate of P. polyphylla var. yunnanensis leaves under various water conditions, but double inoculation with AMF + PGPE greatly increased the intercellular CO2concentration and chlorophyll fluorescence parameter(Fv/Fm). Under diverse water treatments, single inoculation AMF had the highest proline content, whereas double inoculation AMF + PGPE may greatly improve the amount of abscisic acid(ABA) and indoleacetic acid(IAA) compared to normal water under moderate drought. Double inoculation AMF + P

关键词： arbuscular mycorrhizal fungi gene expression Paris polyphylla Smith var.yunnanensis (Franch.)Hand.-Mazz plant growth-promoting endophytes steroidal saponin

Studies on New steroidal saponins from Allii macrostemonis Bulbus and Their Antitumor Activities

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Chemical Research in Chinese Universities 2018年第1期34卷 61-66页

作者： YAO Hua WANG Tong WU Qian LIU Ying LI Peng LI Xuwen JIN Yongri Scienttfic Research Center China-Japan Union Hospital of Jilin University Changchun 130033 P. R. China College of Chemistry Jilin University Changchun 130012 P. R. China

Two new steroidal saponms（2, 3） and two known compounds（1, 4） were isolated from 75% ethanol extract ofAllii macrostemonis bulbus by various spectral methods. The two new steroidal saponins were respectively elucidated as （25S）-26-O-β-D-glucopyranosyl-5α-furostanol-2α,3β,22,26-tetrol-3-O-β-D-glucopyranosyl（1→2）-[β-D- glucopyranosyl（1→3）]-β-D-glucopyranosyl（1→4 ）-β-D-galactopyranoside（2 ） and 26-O-β-D-glucopyranosyl-5α- furostanol-25（ 27）-ene-3β,22,26-tetrol-3-O-β-D-glucopyranosyl（ 1→2 ）-[β-D-glucopyranosyl（ 1→3 ）] -β-D-glucopyranosyl- （1→4）-β-D-galactopyranoside（3）. Compound 4 was obtained from this medical plant for the first time. The activity experiments of proliferation inhibition on tumor cells were performed by cell counting kit-8（CCK-8） method for compound 2（25S-configuration） and its isomer（compound 1, 25R-configttration）. The results show that compounds 1 and 2 have weak inhibition on lung cancer A549 cells, melanoma A375 cells and breast cancer MCF-7 cells as well as certain inhibitory effect on liver cancer HepG2 cells, and the inhibitory effect of compound 1 is stronger than that of compound 2.

关键词： Allii macrostemonis bulbus steroidal saponin Structure identification Proliferation inhibition Cell counting kit-8(CCK-8) method

Two new steroidal saponins isolated from Anemarrhena asphodeloides

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Chinese Journal of Natural Medicines 2017年第3期15卷 220-224页

作者： SUN Xing-Huan ZHU Fu-Tao ZHANG Yu-Wei CHEN Fang-Fang YU Yun SONG Ning-Ning HUANG Xue-Feng State Key Laboratory of Natural Medicines Department of Natural Medicinal ChemistryChina Pharmaceutical UniversityNanjing 210009China

Two new steroidal saponins,named timosaponin P(1) and timosaponin Q(2),were isolated from the rhizome parts of Anemarrhena asphodeloides Bunge using various chromatographic *** structures and absolute configurations were elucidated by a combination of spectroscopic and spectrometric data,including 1D,2D NMR,HR-ESI-MS and ECD calculations,and this is the first time the absolute configuration of C-23 of steroidal saponin was confirmed by ECD calculations.

关键词： Anemarrhena asphodeloides Timosaponin P Timosaponin Q ECD steroidal saponin Absolute configuration

The structural elucidation of two new artificial steroidal saponins

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Chinese Chemical Letters 2012年第3期23卷 332-334页

作者： Bin Wu Zhi Yu Liu Ming Song Fan Zhao Lin Sun Wei Xin Jiang Cheng Gang Huang Shanghai Institute of Materia Medica Chinese Academy of SciencesShanghai 201203China Harbin University of Commerce Harbin 150076China

Two novel steroidal saponins（timosaponin BⅢ-a,timosaponin BⅢ-b） together with a known steroidal saponin（timosaponin BⅡ-b） were prepared by the dilute acid hydrolysis of timosaponin BⅢ.Their structures were elu... 详细信息

关键词： Rhizoma anemarrhenae steroidal saponin Timosaponin BⅢ Acid hydrolysis NMR

Two New steroidal saponins from Diuranthera inarticulata

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Chinese Chemical Letters 2001年第2期12卷 143-144页

作者： Wei Li YANG Jun TIAN Qiang ZHANG Shu Lin PENG Li Sheng DING Chengdu Institute of Biology Chinese Academy of SciencesChengdu 610041 School of Pharmacy West China University of Medical SciencesChengdu 610041

Two new steroidal saponins, diuranthosides D and E, were isolated from the whole plant of Diuranthera inarticulata Wang et K. Y. Lang. By means of spectral and chemical analysis, the structure of the new compounds were established as neotigogenin-3-O-(-D-glucopyranosyl (1?3)-(-D-xylopyranosyl-(1?3)-[(-D-glucopyranosyl(1?2)]-(-D-glucopyranosyl(1?4)-(-D-galactopyranoside (1) and neotigogenin-3-O-(-D-glucopyranosyl(1?3)-(-D-glucopyranosyl (1?3)-(-D-xylopyranosyl(1?3)-[(-D-glucopyranosyl(1?2)]-(-D-glucopyranosyl(1?4)-(-D-galactopyranoside (2) respectively.

关键词： Diuranthera inarticulata Liliaceae steroidal saponin diuranthosides D and E

Simultaneous determination of six steroidal saponins and one ecdysone in Asparagus filicinus using high performance liquid chromatography coupled with evaporative light scattering detection

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Acta Pharmaceutica Sinica B 2012年第3期2卷 267-273页

作者： Libo Zhou Zhihong Cheng Daofeng Chen Department of Pharmacognosy School of PharmacyFudan UniversityShanghai 201203China

A high-performance liquid chromatography coupled with an evaporative light scattering detector(HPLC-ELSD)has been developed to evaluate the quality of Asparagus filicinus through a simultaneous determination of six steroidal saponins and one ecdysone,including aspafiliosides A,B,C,E,G,filiasparoside A and *** a C_(18) analytical column,the seven analytes were separated efficiently using acetonitrile-water as the mobile phase in a gradient *** method limits of detection ranged 0.1250.225μg,and the method limits of quantitation ranged 0.408-0.720μg,*** intra-and inter-day precisions of the method were evaluated and were all less than 3%.All the recoveries for the spiked analytes ranged 95.16%-100.61%.The proposed method was succesfully applied to quantify the seven components in thirteen samples from different localities in China.

关键词： HPLC-ELSD Asparagus filicinus steroidal saponin Ecdysone Aspafiliosides Filiasparoside A 20-Hydroxyecdysone Quality evaluation

A New Furostanol saponin from the Water-extract of Dioscorea nipponica Mak.,the Raw Material of the Traditional Chinese Herbal Medicine Wei Ao Xin

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Chinese Chemical Letters 2004年第10期15卷 1191-1194页

作者： ChengBinCUI ChiXU QianQunGU ShiDongCHU HaiHongJI GangJING MarineDrugandFoodInstitute OceanUniversityofChinaQingdao266003 TOPSUNGroupCO. LTDXi＇an710075

26-O-β-D-Glucopyranosyl-furost-5(6),20(22)-dien-3β,26-diol 1, a new furostanol saponin, was isolated from the water-extract of Dioscorea nipponica Mak., the raw material of a traditional Chinese herbal medicine Wei Ao Xin. The structure of 1 was determined on the basis of its spectral data especially by NMR spectroscopy. The result provides the first example of naturally occurring furostanol saponins with a single saccharide chain at the C-26 position.

关键词： O-a-D-Glucopyranosyl-furost-5(6) 20(22)-dien-3a 26-diol furostanol saponin steroidal saponin structure NMR Dioscorea nipponica Dioscoreaceae.

Chemical Constituents of the Roots of Vernonia cumingiana Benth.

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Journal of Integrative Plant Biology 2005年第8期47卷 1016-1020页

作者： Qing-Hua LIU Jing-Quan YUAN Mao-Rong SUO Jun-Shan YANG Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100094 China Guangxi Botanical Garden of Medicinal Plants Nanning 530023 China

To search for new and bioactive constituents from traditional Chinese medicines, a new steroidal saponin, named vernonioside G （1）, was isolated from the roots of Vernonia cumingiana Benth. （Compositae）. The structure of vernonioside G was elucidated using spectral methods, particularly two-dimensional nuclear magnetic resonance analysis. Together with the new compound, eight known compounds were also isolated and identified from the roots of V. cumingiana, among which, VE-1 （2） and 24-methylenelanost-9（11）- en-3β-ol acetate （3） were assigned NMR data for the first time and compound 3 was obtained as a natural product from a plant for the first time.

关键词： Compositae steroidal saponin Vernonia cumingiana vernonioside G.

Polyphyllin Ⅰ: A new potential antitumor agent from Rhizoma Paridis

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Polyphyllin Ⅰ: A new potential antitumor agent from Rhizoma...