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Asian Journal of Andrology 2022年第1期24卷 62-66页

作者： Yong Zhu Feng Zhang Hua Cheng Xiao-Xi Sun Feng Jiang Obstetrics and Gynecology Hospital Fudan UniversityShanghai 200011China Shanghai Ji Ai Genetics and IVF Institute Obstetrics and Gynecology HospitalFudan UniversityShanghai 200011China

For infertility treatment,the selection of in vitro fertilization(IVF)or intracytoplasmic sperm injection(ICSI)is decided by multiplying indicators(including fallopian tube factors,semen count,and semen motility),except for sperm morphology.In this study,we conducted a retrospective analysis,from implantation to birth,over a period of 5 years.A total of 1873 couples with primary or secondary fallopian tube factors and an increased defective sperm morphology rate(DSMR)were divided into different groups to receive IVF or ICSI cycles.By comparing the outcomes,we found that the F1 group(DSMR98%,IVF group 3;P98%,ICSI group 3)had higher cleavage rate(Psperm morphology should also be considered when selecting IVF or ICSI combined with other semen parameters before the first assisted reproductive technologies(ART)cycle,especially for males with severe sperm defects.

关键词： clinical outcome in vitro fertilization intracytoplasmic sperm injection sperm morphology

Semen analysis with regard to and functional aspects sperm number, sperm morphology

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Asian Journal of Andrology 2010年第1期12卷 26-32页

作者： Rune Eliasson Bjornstigen 13 SE 165 71 HASSELBY Sweden

The new World Health Organization （WHO） Manual for Semen Analysis contains several improvements. One is that the 20 million spermatozoa per mL paradigm has been ousted in favour of proper calculations of lower reference limits for semen from men, whose partners had a time-to-pregnancy of 12 months or less. The recommendation to grade the progressive motility as described in the third and fourth editions of the WHO manual was not evidence-based, and WHO was therefore motivated to abandon it. However, the new recommendation is not evidence-based either, and it is difficult to understand the rational for the new assessment. It may have been a compromise to avoid returning to the rather robust system recommended in the first edition （1980）. The unconditional recommendation of the ＇Tygerberg strict criteria＇ is not evidence-based, and seems to be the result of an unfortunate bias in the composition of the Committee in favour of individuals known to support the ＇strict criteria＇ method. This recommendation will have negative effects on the develop- ment ofandrology as a scientific field. Given the importance of the WHO manual, it is unfortunate that the recommenda- tions for such important variables, as motility and morphology, lack evidence-based support.

关键词： evaluation report semen analysis spermatozoa sperm morphology sperm motility

Effect of lead on survival, locomotion and sperm morphology of Asian earthworm, Pheretima guillelmi

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Journal of Environmental Sciences 2009年第5期21卷 691-695页

作者： ZHENG Rongquan, LI Canyang College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China. College of Chemistry and Life Science Zhejiang Normal University Jinhua 321004 China

To provide basic toxicity data for formulating risk characterization benchmarks, the effects of lead on survival, locomotion, and sperm morphology were investigated in the Asian earthworm Pheretima guillelmi. The LC50 of P. guiUelmi for 7 and 14 d were 4285＋339 mg/kg and 3207＋248 mg/kg, which shows P. guillelmi can tolerate a higher concentration of lead nitrate. The average weight of the surviving earthworms decreased at concentration of 2800 mg Pb/kg soil, and the locomotor ability of earthworms exposed to a range of soil Pb concentrations showed a general decrease with increasing Pb concentrations. We also presented data depicting the sperm morphology of earthworms, which shows potential as a sensitive biomarker for measuring the effects of heavy metal on reproduction.

关键词： lead Pheretima guillelmi sperm morphology locomotion

sperm collection in Black-legged Kittiwakes and characterization of sperm velocity and morphology

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Avian Research 2019年第1期10卷 89-100页

作者： Ségolène Humann?Guilleminot Pierre Blévin Antonin Azou?Barré Agathe Yacoumas Geir Wing Gabrielsen Olivier Chastel Fabrice Helfenstein Laboratory of Evolutionary Ecophysiology Institute of BiologyUniversity of Neuchatel Centre d'Etudes Biologiques de Chizé(CEBC) UMR 7372-CNRSUniversité de La Rochelle Norwegian Polar Institute Fram Centre

Background:Collecting and studying live sperm is central to many important fields of biology.Yet,a simple method to collect live sperm is lacking in wild seabird species.Here,we describe a non?invasive method to collect viable sperm samples based on a simple massage technique applied to male Black?legged Kittiwakes(Rissa tridactyla).Methods:We studied a colony breeding at Kongsfjorden,Svalbard and successfully obtained sperm samples from 32 males.With a subset of samples(n = 12 males),we compared the suitability of several extenders(0.9% NaCl,PBS,Earle's balance salt solution,Dulbecco's modified Eagle medium) in maintaining sperm alive long enough for analyses.With another 18 ejaculates,we conducted computer assisted sperm analyses using the CASA plugin for ImageJ.We provide details about the settings to be used for such analyses.Lastly,droplets from 20 ejaculates were smeared on glass slides and preserved with formalin to characterize sperm morphology in terms of total sperm length,sperm head length,midpiece length and flagellum length,and percentage of abnormal sperm.Results:With this method and under field conditions,we were able to obtain sufficient amounts of live sperm to assess traits related to sperm quality(e.***.sperm morphology,percentage of motile sperm,sperm velocity).We found that two extenders,Earle's balanced salt solution and Dulbecco modified Eagle's medium,yielded similarly good results.Additionally,we investigated whether specific behaviours were associated with successful sperm collection and whether sperm collection success depended on how long before laying sperm collection was attempted.Finally,we provide mean values for sperm morphology,sperm swimming ability and percentage of motile sperm,which may prove useful for future comparative analyses,and we report high levels of sperm abnormality and within?ejaculate variation in sperm morphology.Conclusions:We discuss the high percentage of abnormal sperm and high within?ejaculate variation in sperm morphology in light of sperm competition theory

关键词： Black-legged Kittiwakes sperm spermatozoa Semen collection Non?invasive method Larids sperm velocity sperm morphology Abnormal sperm

The influence of Mycoplasma species on human and canine semen quality:a review

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Asian Journal of Andrology 2023年第1期25卷 29-37页

作者： Kinga Domrazek Ilona Kaszak Szymon Kanafa Mariusz Sacharczuk Piotr Jurka Laboratory of Small Animal Reproduction Institute of Veterinary MedicineWarsaw University of Life SciencesNowoursynowska 159C StreetWarsaw 02-787Poland Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences Jastrz biec Postepu Street 36AMagdalenka 05-552Poland

Mycoplasma species(spp.)are bacteria that are difficult to detect.Currently,the polymerase chain reaction(PCR)is considered the most effective diagnostic tool to detect these microorganisms in both human and veterinary medicine.There are 13 known species of human Mycoplasma and 15 species of canine Mycoplasma.Owing to the difficulties in identifying the individual species of Mycoplasma,there is a lack of information regarding which species are saprophytic and which are pathogenic.The prevalence of the individual species is also unknown.In addition,in both humans and dogs,the results of some studies on the impact of Mycoplasma are conflicting.The presence of Mycoplasma spp.on the epithelium of reproductive tract is often associated with infertility,although they are also detected in healthy individuals.The occurrence of Mycoplasma spp.is more common in dogs(even 89%)than in humans(1.3%–4%).This is probably because the pH of a dog's genital is more conducive to the growth of Mycoplasma spp.than that of humans.Phylogenetically,human and canine Mycoplasma are related,and majority of them belong to the same taxonomic group.Furthermore,40%of canine Mycoplasma spp.are placed in common clusters with those of human.This suggests that species from the same cluster can play a similar role in the canine and human reproductive tracts.This review summarizes the current state of knowledge about the impact of Mycoplasma on canine and human male fertility as well as the prospects of further development in this field.

关键词： Mycoplasma sperm morphology sperm motility

The effect of age and abstinence time on semen quality:a retrospective study

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Asian Journal of Andrology 2022年第1期24卷 73-77页

作者： Gang-Xin Chen Hai-Yan Li Yun-Hong Lin Zhi-Qing Huang Peng-Yu Huang Lin-Cui Da Hang Shi Lei Yang Ye-Bin Feng Bei-Hong Zheng Center of Reproductive Medicine Fujian Maternity and Child Health HospitalAffiliated Hospital of Fujian Medical UniversityFuzhou 350001China

This study analyzed the effects of male age and abstinence time on semen quality and explored the best abstinence time for Chinese males among different age groups.Semen parameters,including sperm kinetics,morphology,and DNA fragmentation index(DFI),were reviewed from 2952 men.Samples were divided into six age groups(≤25 years,26–30 years,31–35 years,36–40 years,41–45 years,and>45 years)and were divided into six groups according to different abstinence time(2 days,3 days,4 days,5 days,6 days,and 7 days).The differences in semen quality between the groups were compared,and the effect of age and abstinence time on semen quality was analyzed.Significant differences were observed in semen volume,progressive motility(PR),and DFI among the age groups(all Psperm morphological parameters(all P>0.05).There were significant differences in semen volume,PR,and DFI among different abstinence time groups(all Psperm morphological parameters(all P>0.05).Pearson analysis showed that male age and abstinence time were both significantly correlated with sperm kinetics and DFI(both Psperm morphological parameters(all P>0.05).The box plots and histograms of men’s age,abstinence time,and semen quality show that most semen quality parameters differ significantly between the 2 days and 7 days abstinence groups and other groups at different ages.Except for the sperm morphology parameters,sperm kinetic parameters and sperm DFI are linearly related to male age and abstinence time.

关键词： abstinence time age DNA fragmentation index sperm kinetics sperm morphology

The relationship between DNA fragmentation and the intensity of morphologically abnormal human spermatozoa

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Asian pacific Journal of Reproduction 2024年第1期13卷 22-27页

作者： Mercedes González-Martínez Pascual Sánchez-Martín Carmen López-Fernández Stephen D.Johnston Jaime Gosálvez Clínica Ginemed Unidad de ReproducciónSevillaSpain Department of Biology Universidad Autónoma de MadridCantoblancoMadridSpain School of Environment The University of QueenslandGattonAustralia School of Veterinary Science The University of QueenslandGattonAustralia

Objective:To determine the relationship between teratozoospermia and sperm DNA fragmentation(SDF)in the human ejaculate.Methods:This retrospective study included 100 normozoospermic men as a control cohort(abnormal forms>14%),210 patients with a high level of abnormal forms(≤4%)and 65 patients presenting with a moderate level of abnormal forms(>4%to≤14%)based on the World Health Organization definitions.sperm morphology was assessed using bright field microscopy.sperm DNA fragmentation was assessed using the sperm chromatin dispersion assay.Non-parametric analyses were conducted to examine the relationship between abnormal sperm morphology and sperm DNA fragmentation;receiver operating characteristic(ROC)analyses were conducted to assess sensitivity and specificity of this relationship.Results:A correlation analysis revealed that the higher the proportion of abnormal spermatozoa in the ejaculate,the higher the level of SDF(Spearman's Rho=-0.230;Pspermia would help further clarify any causal relationship with SDF,there is clearly a synergistic or coincident affiliation between these variables that needs to be acknowledged by the clinician when interpreting the spermiogram.

关键词： Teratozoospermia sperm morphology sperm DNA fragmentation Male factor Human reproduction Human fertility

Ameliorative effect of Punica granatum on sperm parameters in rats exposed to mobile radioelectromagnetic radiation

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Asian pacific Journal of Reproduction 2021年第5期10卷 225-231页

作者： Anjaneyababu Naik Banavath Sridevi Nangali Srinivasa Department of Anatomy Sri Devaraj Urs Medical CollegeSri Devaraj Urs Academy of Higher Education and ResearchTamakaKolarKarnatakaIndia

Objective:To study the effect of mobile emitted radio frequency electromagnetic radiation(RF-EMR)on sperm parameters(sperm count,viability,motility,progressivity,and morphology)and ameliorative effect of pomegranate juice in rats.Methods:Thirty male Sprague Dawley rats were divided into five groups(n=6 in each group).GroupⅠwas neither exposed to RF-EMR nor given pomegranate juice,groupⅡ,ⅢandⅣwere exposed to mobile emitted RF-EMR for 60 min/day for 90 days.After 90-day exposure to RF-EMR,groupⅢwas supplemented with pomegranate juice for 90 days(1 mL/day)and groupⅣwas allowed to recover for 90 days without supplementation of pomegranate juice.GroupⅤwas supplemented with pomegranate juice for 90 days without exposure to RF-EMR.At the end of intervention,epididymal sperm parameters(sperm count,viability,motility,progressivity,and morphology)were measured.Results:The microscopic examination of sperm parameters such as sperm count,sperm viability,sperm motility,progressivity were significantly decreased in groupⅡ(the mobile RF-EMR group)compared to groupⅠ(the control group)(Psperm morphology was also significantly altered(abnormal)in groupⅡcompared to groupⅠ(Psperm parameters including sperm morphology were significantly altered in groupⅢ(mobile RF-EMR+pomegranate juice)compared to groupⅡ(Psperm parameters including sperm morphology were not significantly altered in groupⅣ(the mobile RF-EMR recovery group)compared to groupⅡ(P>0.05).The sperm parameters were non-significantly increased in group V(the pomegranate juice group)compared to groupⅠ(P>0.05).Conclusions:Mobile RF-EMR exposure reduces the sperm parameters,which,however,can be reversed by treatment with pomegranate juice,indicating that pomegranate juice can be used as a nutritional supplement to improve sperm quality.

关键词： Mobile radioelectromagnetic radiation Pomegranate juice sperm parameters Male infertility Anthocyanin spermatocytogenesis Epididymis sperm morphology

Practical semen analysis： from A to Z

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Asian Journal of Andrology 2010年第1期12卷 14-20页

作者： Charlene Brazil Center for Health and the Environment University of California Davis CA 95616 USA

Accurate semen analysis is critical for decisions about patient care, as well as for studies addressing overall changes in semen quality, contraceptive efficacy and effects of toxicant exposure. The standardization of semen analysis is very difficult for many reasons, including the use of subjective techniques with no standards for comparison, poor technician training, problems with proficiency testing and a reluctance to change techniques. The World Health Organization （WHO） Semen handbook （2010） offers a vastly improved set of standardized procedures, all at a level of detail that will preclude most misinterpretations. However, there is a limit to what can be learned from words and pictures alone. A WHO- produced DVD that offers complete demonstrations of each technique along with quality assurance standards for motility, morphology and concentration assessments would enhance the effectiveness of the manual. However, neither the manual nor a DVD will help unless there is general acknowledgement of the critical need to standardize techniques and rigorously pursue quality control to ensure that laboratories actually perform techniques ＇according to WHO＇ instead of merely reporting that they have done so. Unless improvements are made, patient results will continue to be compromised and comparison between studies and laboratories will have limited merit.

关键词： andrology laboratory quality assurance semen analysis sperm concentration sperm morphology sperm motility

Impact of chronological ageing on semen parameters in southern Indian men visiting infertility centre:A retrospective study

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Asian pacific Journal of Reproduction 2023年第1期12卷 10-15页

作者： Muhammed Asif Asha S.Vijay Maheshwari Syed Fyzullah Usha Rani Swathi R Damodara Gowda KM GarbhaGudi Institute of Reproductive Health and Research(GGIRHR) HanumanthnagarBangaloreKarnataka-560004India Nitte(Deemed to be University) KS Hegde Medical AcademyDepartment of PhysiologyMangalore-575018KarnatakaIndia

Objective:To investigate the association between age and semen parameters among male partners of subfertile couples.Methods:This retrospective study analyzed the semen of 1523 infertile men aged 26 to 50 years.Data were extracted from GarbhaGudi IVF Centre database from January 2019 to September 2020.The basic semen parameters were interpreted according to the WHO manual 2021,6th edition.Semen parameters in different age groups were compared.Results:Total and progressive motile sperms were significantly higher in the age group of 26-30 years compared to other age groups(Psperm count was significantly higher in the age group of 26-30 years compared to the age groups of 41-45 years and>46 years(P=0.001).However,sperm head defects,neck and midpiece defects,tail defects,and cytoplasmic droplets showed statistically insignificant difference in all the age groups(P>0.05).Semen viscosity showed no statistical difference in all the age groups compared to the reference age group of 26 to 30 years.Conclusions:Higher age can lead to a significant decrease in normal sperms and motility in subfertile men.Hence,male partner age should be considered as one of the major determining factors for reproductive outcomes.

关键词： Age Semen analysis sperm count sperm morphology Motility spermatozoa Infertility Male