检索结果-南通市图书馆

sperm dna damage in men from infertile couples

Asian Journal of Andrology 2008年第5期10卷 786-790页

作者： Juris Erenpreiss Saad Elzanaty Aleksander Giwercman Andrology Laboratory Riga Stradins University LV-1007 Riga Latvia Reproductive Medicine Centre Scanian Andrology Centre Malmo University Hospital Malmo SE 205 02 Sweden Department of General Surgery Urology Section Kristianstad Central Hospital Kristianstad SE 291 85 Sweden

Aim： To investigate the prevalence of high levels of sperm dna damage among men from infertile couples with both normal and abnormal standard semen parameters. Methods： A total of 350 men from infertile couples were assessed. Standard semen analysis and sperm chromatin structure assay （SCSA） were carried out. Results： Ninety-seven men （28% of the whole study group） had a dna fragmentation index （DFI）〉 20%, and 43 men （12%） had a DFI 〉 30%. In the group of men with abnormal semen parameters （n = 224）, 35% had a DFI 〉 20%, and 16% had a DFI 〉 30%, whereas these numbers were 15% and 5%, respectively, in the group of men with normal semen parameters （n = 126）. Men with low sperm motility and abnormal morphology had significantly higher odds ratios （ORs） for having a DFI 〉 20% （4.0 for motility and 1.9 for morphology） and DFI 〉 30% （6.2 for motility and 2.8 for morphology） compared with men with normal sperm motility and morphology. Conclusion： In almost one-third of unselected men from infertile couples, the DFI exceeded the level of 20% above which, according to previous studies, the in vivo fertility is reduced. A significant proportion of men with otherwise normal semen parameters also had high sperm dna damage levels. Thus, the SCSA test could add to explaining causes of infertility in cases where semen analysis has not shown any deviation from the norm. We also recommend running the SCSA test to choose the appropriate assisted reproductive technique （ART）.

关键词： infertility sperm dna damage sperm chromatin structure assay semen quality

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A systematic review and meta-analysis to determine the effect of sperm dna damage on in vitro fertilization and intracytoplasmic sperm injection outcome

引用

Asian Journal of Andrology 2017年第1期19卷 80-90页

作者： Luke Simon Armand Zini Alina Dyachenko Antonio Ciampi Douglas T Carrell Department of Surgery (Urology) University of Utah School of Medicine Salt Lake City UT USA Division of Urology Department of Surgery St. Mary's Hospital Center St. Mary's Hospital 3830 Lacombe Avenue Montreal Quebec H3T 1M5 Canada Department of Human Genetics University of Utah School of Medicine Salt Lake City UT USA Department of Obstetrics and Gynecology University of Utah School of Medicine Salt Lake City UT USA.

sperm dna damage is prevalent among infertile men and is known to influence natural reproduction. However, the impact of sperm dna damage on assisted reproduction outcomes remains controversial. Here, we conducted a meta-analysis of studies on sperm dna damage （assessed by SCSA, TUNEL, SCD, or Comet assay） and clinical pregnancy after IVF and/or ICSI treatment from MEDLINE, EMBASE, and PUBMED database searches for this analysis. We identified 41 articles （with a total of 56 studies） including 16 IVF studies, 24 ICSI studies, and 16 mixed （IVF ＋ ICSI） studies. These studies measured dna damage （by one of four assays： 23 SCSA, 18 TUNEL, 8 SCD, and 7 Comet） and included a total of 8068 treatment cycles （3734 IVF, 2282 ICSI, and 2052 mixed IVF ＋ ICSI）. The combined OR of 1.68 （95% Ch 1.49-1.89; P 〈 0.0001） indicates that sperm dna damage affects clinical pregnancy following IVF and/or ICSI treatment. In addition, the combined OR estimates of IVF （16 estimates, OR = 1.65; 95% CI： 1.34-2.04; P 〈 0.0001）, ICSI （24 estimates, OR = 1.31; 95% Ch 1.08-1.59; P = 0.0068）, and mixed IVF ＋ ICSI studies （16 estimates, OR = 2.37; 95% Ch 1.89-2.97; P〈 0.0001） were also statistically significant. There is sufficient evidence in the existing literature suggesting that sperm dna damage has a negative effect on clinical pregnancy following IVF and/or ICSI treatment.

关键词： assisted reproductive technology outcomes clinical pregnancy meta-analysis sperm dna damage systematic review

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Aberrant protamination in sperm correlates to anomalous nuclear and cytoplasmic architectures in infertile males with sperm dysmorphology

引用

Asian Journal of Andrology 2024年第2期26卷 183-188页

作者： Huan Jiang Chu-Jie Huang Department of Reproductive Endocrinology Longgang District Maternity&Child Healthcare Hospital of Shenzhen City(Longgang Maternity and Child Institute of Shantou University Medical College)Shenzhen 518172China Institute of Reproductive Immunology College of Life Science and TechnologyJinan UniversityGuangzhou 510632China

Aberrant sperm protamination is linked to sperm dysmorphology and nuclear chromatin condensation.Yet,its effects on sperm cytoplasmic maturation remain largely unexplored.The relationships of protamines,sperm morphology,dna damage,and cytoplasmic remodeling were illustrated in this study to provide fresh perspectives on the mechanisms of male infertility.A total of 205 infertile males were allocated into 5 groups according to the percentage of spermatozoa exhibiting abnormal morphology within their samples.sperm concentration,motility,abnormal sperm morphology,cytoplasmic droplets(CDs),and excess residual cytoplasm(ERC)were analyzed according to the World Health Organization manual(2010).sperm nuclear vacuoles(NVs)were determined by propidium iodide(PI)staining.sperm protamine expressions(P1 and P2)were detected by western blot.dna damage was measured by acridine orange test(AOT)to calculate the proportion of sperm with single-strand dna breaks(SSBs).Our data showed that sperm concentration and motility in infertile males significantly decreased with the severity of abnormal sperm morphology(both Psperm dysmorphology,whilst the P2 level decreased(all Psperm dysmorphology and positively correlated with the SSB rate(all Psperm dysmorphology and compromise male fertility by impairing sperm's nucleus and cytoplasm maturation,with the P1/P2 ratio potentially serving as a valuable indicator of sperm quality and male fertility.

关键词： abnormal sperm morphology excess residual cytoplasm male infertility protamines sperm dna damage sperm nuclear vacuoles

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Novel association between sperm deformity index and oxidative stress-induced dna damage in infertile male patients

引用

Asian Journal of Andrology 2005年第2期7卷 121-126,页

作者： Tamer M.Said Nabil Aziz Rakesh K.Sharma Iwan Lewis-Jones AnthonyJ.ThomasJr AshokAgarwal Center for Advanced Research in Human Reproduction Infertility and Sexual FunctionGlickman Urological Institute and Department of Obstetrics-GynecologyThe Cleveland Clinic FoundationClevelandOhio 44195USA Reproduction Medicine Unit Liverpool Women's HospitalLiverpool L87SSSUK

Aim:To investigate the impact of abnormal sperm morphology using the sperm deformity index (SDI) on reactive oxygen species (ROS) production and its correlation with sperm dna damage.Methods:Semen samples were collected from men undergoing infertility screening (n=7) and healthy donors (n=6).Mature spermatozoa were isolated and incubated with 5 mmol/L β-nicotinamide adenine dinucleotide phosphate (NADPH) for up to 24 h to induce ROS.sperm morphology was evaluated using strict Tygerberg's criteria and the SDI.ROS levels and dna damage were assessed using chemiluminescence and terminal deoxynucleotidyl transferase-mediated fluorescein- dUTP nick end labeling (TUNEL) assays,respectively.Results:SDI values (median [interquartiles]) were higher in patients than donors (2 [1.8,2.1] vs.1.53 [1.52,1.58],P=0.008).Aliquots treated with NADPH showed higher ROS levels (1.22 [0.30,1.87] vs.0.39 [0.10,0.57],P=0.03) and higher incidence of dna damage than those not treated (10 [4.69,24.85] vs.3.85 [2.58,5.10],P=0.008).Higher dna damage was also seen following 24 h of incubation in patients compared to donors.SDI correlated with the percentage increase in sperm dna damage following incubation for 24 h in samples treated with NADPH (r=0.7,P=0.008) and controls (r=0.58,P=0.04). Conclusion:SDI may be a useful tool in identifying potential infertile males with abnormal prevalence of oxidative stress (OS)-induced dna damage.NADPH plays a role in ROS-mediated sperm dna damage,which appears to be more evident in infertile patients with semen samples containing a high incidence of morphologically abnormal spermatozoa.

关键词： β-nicotinamide adenine dinucleotide phosphate oxidative stress sperm deformity index sperm dna damage

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Novel insights into the pathophysiology of varicocele and its association with reactive oxygen species and sperm dna fragmentation

引用

Asian Journal of Andrology 2016年第2期18卷 186-193,J0003页

作者： Chak-Lam Cho Sandro C Esteves Ashok Agarwal Division of Urology Department of Surgery Kwong Wah Hospital 25 Waterloo Road Yau Ma Tei Hong Kong ANDROFERT Center for Male Reproduction Av. Dr. Heitor Penteado 1464 campinas SP 13075-460 Brazil American Center for Reproductive Medicine 10681 Carnegie Avenue X-11 Cleveland OH 44195 USA.

Varicocele has been associated with reduced male reproductive potential. With the advances in biomolecular techniques, it has been possible to better understand the mechanisms involved in testicular damage provoked by varicocele. Current evidence suggests the central role of reactive oxygen species （ROS） and the resultant oxidative stress （OS） in the pathogenesis of varicocele-associated male subfertility although the mechanisms have not yet been fully described and it is likely to be multifactorial. Excessive ROS is associated with sperm dna fragmentation, which may mediate the clinical manifestation of poor sperm function and fertilization outcome related to varicocele. Testing of ROS/OS and dna fragmentation has the potential to provide additional diagnostic and prognostic information compared to conventional semen analysis and may guide therapeutic management strategies in individual patient.

关键词： antioxidants infertility male reproduction oxidative stress sperm dna damage sperm dna fragmentation varicocele varicocelectomy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Minimal volume vitrification of epididymal spermatozoa results in successful in vitro fertilization and embryo development in mice

引用

Asian Journal of Andrology 2017年第1期19卷 107-112页

作者： Fabrizzio Horta Hamida Alzobi Sutthipat Jitanantawittaya Sally Catt Penny Chen Mulyoto Pangestu Peter Temple-Smith Education Program in Reproduction and Development Department Obstetrics and Gynaecology Monash University Clayton VlC 3168 Australia Department of Obstetrics and Gynaecology Unit of Reproductive Medicine Clinica Las Condes Lo Fentecilla 441 Las Condes Santiago Region Metropolitana 7550000 Chile.

This study compared three cryopreservation protocols on sperm functions, IVF outcomes, and embryo development. Epididymal spermatozoa cryopreserved using slow-cooling （18% w/v raffinose, RS-C） were compared with spermatozoa vitrified using 0.25 M sucrose （SV） or 18% w/v raffinose （RV）. The motility, vitality, and dna damage （TUNEL assay） of fresh control （FC） spermatozoa were compared with post-thawed or warmed RS-C, RV, and SV samples. Mouse oocytes （n = 267） were randomly assigned into three groups for insemination： RV （n = 102）, RS-C （n = 86）, and FC （n = 79）. The number and the proportion of two-cell embryos and blastocysts from each treatment were assessed. sperm motility （P 〈 0.01） and vitality （P 〈 0.05） were significantly reduced after vitrification compared with slow-cooled spermatozoa. However, dna fragmentation was significantly reduced in spermatozoa vitrified using sucrose （15 - 1.8% [SV] vs 26 - 2.8% [RV] and 27 - 1.2% [RS-C]; P 〈 0.01）. Although the number of two-cell embryos produced by RS-C, RV, and FC spermatozoa was not significantly different, the number of blastocysts produced from two-cell embryos using RV spermatozoa was significantly higher than FC spermatozoa （P = 0.0053）. This simple, small volume vitrification protocol and standard insemination method allows successful embryo production from small numbers of epididymal spermatozoa and may be applied clinically to circumvent the need for ICSI, which has the disadvantage of bypassing sperm selection.

关键词： cryopreservation epididymal sperm in vitro fertilization/intracytoplasmic sperm injection reproductive techniques sperm dna damage vitrification

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Supplementation with L-Carnitine Rescues sperm Epigenetic Changes in Asthenospermic Male Semen with Altered Acetyl-L-Carnitine Levels

引用

Reproductive and Developmental Medicine 2020年第3期4卷 146-155页

作者： Xiao-Hui Jiang Chuan Jiang Lin Yu Xiao-Liang Li Tao Zuo Pei-Fei Gu Fu-Ping Li Wen-Ming Xu Human sperm Bank West China Second University Hospital of Sichuan UniversityChengdu 610041China Key Laboratory of Birth Defects and Related Diseases of Women and Children Ministry of EducationWest China Second University HospitalSichuan UniversityChengdu 610041China Reproductive Endocrinology and Regulation Laboratory West China Second University HospitalSichuan UniversityChengdu 610041China Department of Obstetrics and Gynecology West China Second University HospitalSichuan UniversityChengdu610041China Northeast Pharmaceutical Group Shenyang 110023China Department of Sports Medicine Shenyang Sports UniversityShenyang 110101China

Objective:To investigate the relationship between the concentration of L-carnitine in semen and sperm parameters and investigate the epigenetic profile in sperm cell after L-carnitine usage.Methods:From February 2017 to February 2018,46 semen samples from asthenospermic males and 41 semen samples from healthy donors were acquired.Motility parameters were assessed using computer-assisted sperm analysis(CASA,n=78)and the dna fragmentation index(DFI)was evaluated through flow cytometry(n=86),%DFI=%cells outside main population.Other oxidative stress markers,such as reactive oxygen species(ROS)levels(n=86)and the mitochondria dna copy numbers,were detected(n=78).The concentration of L-carnitine and acetyl-L-carnitine was detected(n=82),and methylation was analyzed(n=30).After that,we collected 13 fresh semen samples from asthenospermic males and 23 fresh semen samples from healthy donors.These samples were used in a freeze-thaw model that was used to determine whether adding L-carnitine could change sperm progressive motility(n=23),apoptosis index(n=9),and methylation analysis(n=7).In total,we have done 13 asthenospermia samples for Western blot,and except for the poor Western result,we analyzed 6 samples for H3K9ac detection,7 samples for H3K9m3 and H3K27m3 detection,and immunofluorescence(n=3).Finally,we had recruited 30 volunteers,and they were given oral administration of L-carnitine for 3 months and then collected semen samples at different time points for methylation analysis.Results:The concentration of acetyl-L-carnitine is negatively correlated with the%DFI value(r^2=0.1090;P=0.0026),and the concentration of acetyl-L-carnitine is positively correlated with sperm forward motility(r^2=0.0543;P=0.0458)and ROS(r^2=0.1854;Pspermia(r^2=0.1701;P=0.0066),and the level of acetyl-L-carnitine in asthenospermic semen is significantly lower than the normal group(P=0.0419).In addition,this study indicates that adding L-carnitine signific

关键词： Acetyl-L-Carnitine Asthenospermia Epigenetic sperm dna damage

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The paternal genome and the health of the assistect reproductive technology child

引用

Asian Journal of Andrology 2015年第4期17卷 616-622页

作者： Sheena EM Lewis Kishlay Kumar Centre for Public Health Queen's University Belfast Grosvenor Road BT12 6BJNI UK.

As a number of children born by assisted reproductive technology （ART） are increasing each year across the developed world, the health of such offspring is a matter of public concern. Does the integrity of the paternal genome impact on offspring health？ In societal terms, as birth rates fall, and the Western population become unsustainable, do the benefits outweigh the costs of creating and providing for this ART conceived subpopulation？ There are little data to date to answer these questions. The long-term health of such children has largely been ignored, and success measured only by early （prebirth） outcomes such as embryo quality or pregnancy. However, there are powerful paradigms such as ageing and smoking that give vital clues as to the potential impact of unhealthy spermatozoa on disease risk, mental and physical health, fertility and mortality of these offspring.

关键词： sperm dna damage paternal genome offspring infertility

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：