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imaging of apoptotic HeLa cells by using scanning near-field optical microscopy

Progress in Natural Science:Materials International 2002年第8期 19-24页

作者： Peter von BLANCKENHAGEN Germany Institute of Nanotechnology Karlsruhe D-76021 Research Center Karlsruhe

By using scanning near-field optical microscopy (SNOM), HeLa cells in apoptosis process are imaged with a higher optical resolution beyond the diffraction limit. Since SNOM provides both topographic and transmitted light intensity information of a cell, it can correlate the structural characteristics and optical properties with the spatial position of the apoptotic cells. Wavelength imaging by using near-field spectroscopy shows that there is a great difference in light propagation and absorption in the cell. This unique technique can be applied to the super high resolution imaging of different components in the cell. The observations by near-field optical imaging and near-field spectroscopy indicate an inhomogeneous aggregation of the inner structure in the apoptotic HeLa cells and the change of transmission intensity of light with the apoptosis status.

关键词： scanning near-field optical microscopy HeLa cell apoptosis spectrum imaging tapping mode.

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Ghost imaging for online angiography

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Translational Neuroscience and Clinics 2017年第2期3卷 116-120页

作者： Zhaohua Yang Nan Zhang Yuzhe Sun Yuanjin Yu School of Instrumentation Science and Opto-electronics Engineering Beihang University Department of Neurosurgery Beijing Anzhen Hospital Capital Medical University

Ghost imaging(GI) has characteristics that make it promising for applications in life sciences and other fields, such as its high sensitivity and strong anti-interference compared with traditional imaging. This paper presents a new approach for online angiography using GI. Two signals are correlation-calculated to detect the object image: one of them is the random light field generated by a computer, and the other enters the optical fiber path after being transmitted to the detected object via a modulator. A new approach for the real-time imaging of intravascular flow, vascular wall structures, and components of atherosclerotic plaque is proposed, which has the advantages of a high sensitivity and anti-interference.

关键词： cerebrovascular diseases cardiovascular diseases angiography ghost imaging computational ghost imaging spectrum imaging

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