Imaging of apoptotic HeLa cells by using scanning near-field optical microscopy

作     者：Peter von BLANCKENHAGEN 

作者机构：Germany Institute of Nanotechnology Karlsruhe D-76021 Research Center Karlsruhe 

出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))

年 卷 期：2002年第8期

页      面：19-24页

学科分类：070207[理学-光学] 07[理学] 08[工学] 0803[工学-光学工程] 0702[理学-物理学] 

基　　金：Supported by the National Natural Science Foundation of China (Grant Nos. 19890380, 10074002, 30070388) the Research Fund for the Doctoral Program of Higher Education, Ministry of Education, China 

主　　题：scanning near-field optical microscopy HeLa cell apoptosis spectrum imaging tapping mode. 

摘      要：By using scanning near-field optical microscopy (SNOM), HeLa cells in apoptosis process are imaged with a higher optical resolution beyond the diffraction limit. Since SNOM provides both topographic and transmitted light intensity information of a cell, it can correlate the structural characteristics and optical properties with the spatial position of the apoptotic cells. Wavelength imaging by using near-field spectroscopy shows that there is a great difference in light propagation and absorption in the cell. This unique technique can be applied to the super high resolution imaging of different components in the cell. The observations by near-field optical imaging and near-field spectroscopy indicate an inhomogeneous aggregation of the inner structure in the apoptotic HeLa cells and the change of transmission intensity of light with the apoptosis status.