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Journal of Integrative Plant Biology 2006年第6期48卷 722-731页

作者： Yan-Song Ma Wen-Hui Wang Li-Xia Wang Feng-Ming Ma Pei-Wu Wang Ru-Zhen Chang Li-Juan Qiu Institute of Crop Science Chinese Academy of Agricultural Sciences National Key Facility of Crop Gene Resources and Genetic Improvement Crop Germplasm and Biotechnology Ministry of Agriculture Beijing 100081 China Jilin Agricultural University Changchun 130118 China Northeast of Agricultural University Harbin 150030 China

soybean cyst nematode （SCN; Heterodera glycines） Is one of the most Important pests affecting soybean production. The best method of control of SCN is through the development of resistant cultlvars. However, limited progress has been made in soybean breeding In China because most modern cultlvars have no resistance to SCN. The distribution and phenotype of 432 immune or highly resistant Chinese accessions were surveyed and a primary core collection was selected as a representative sample for further analyses. Using evenly distributed simple sequence repeat markers, five selection methods were applied to the primary core collection and the optimal method was chosen to establish a core collection, which consisted of 28 accessions. These encompassed 70.8% of the ailelic variation present in the overall resistant collection. The 28 accessions differed from the reference resistant accessions at the genomlc level, Indicating that Chinese resistant accessions are distinct from known resistant accessions. This applied core collection provides a rational framework for undertaking diversity surveys, using genetic variation for the investigation of complex traits and for the discovery of novel traits.

关键词： applied core collection resistance simple sequence repeat marker soybean soybean cyst nematode

A fragment of a 70-kDa Heterodera glycines heat shock protein(HgHSP70)interacts with soybean cyst nematode-resistant GmSHMT08

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Journal of Integrative Agriculture 2022年第10期21卷 2973-2983页

作者： LIU Zhi ZHANG Liu-ping ZHAO Jie JIAN Jin-zhuo PENG Huan HUANG Wen-kun KONG Ling-an PENG De-liang LIU Shi-ming State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China MARA–CABI Joint Laboratory for Bio-safety Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

soybean cyst nematode(SCN)Heterodera glycines is considered as the major constraint to soybean *** SHMT08 at Rhg4 locus on chromosome 08,encoding a serine hydroxylmethyltransferase,is a major gene underlying resistance against *** in Peking-type ***,the molecular mechanism underpinning this resistance is less well characterized,and whether Gm SHMT08 could interact with proteins in *** remains *** this study,yeast two-hybrid screening was conducted using Gm SHMT08 as a bait protein,and a fragment of a 70-kDa heat shock protein(Hg HSP70)was screened from *** that exhibited interaction with Gm *** interaction was verified by both GST pull-down and bimolecular fluorescence complementation *** finding reveals Hg HSP70 could be applied as a potential candidate gene for further exploring the mechanism on Gm SHMT08-mediated resistance against SCN ***.

关键词： soybean cyst nematode Heterodera glycines GmSHMT08 HgHSP70 interaction resistance

Comparative Analysis of Gene Expression Profiling Between Resistant and Susceptible Varieties Infected With soybean cyst nematode Race 4 in Glycine max

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Journal of Integrative Agriculture 2014年第12期13卷 2594-2607页

作者： LI Bin SUN Jun-ming WANG Lan ZHAO Rong-juan WANG Lian-zheng The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Laboratory of soybean Biology (Beijing) MOA/Institute of Crop Sciences Chinese Academy of Agricultural Sciences

soybean cyst nematode （SCN） is one of the most devastating pathogen for soybean. Therefore, identiifcation of resistant germplasm resources and resistant genes is needed to improve SCN resistance for soybean. soybean varieties Huipizhiheidou and Wuzhaiheidou were distributed in China and exhibited broad spectrums of resistance to various SCN races. In this study, these two resistant varieties, combined with standard susceptible varieties （Lee and Essex）, were utilized to identify the differentially expressed transcripts after infection with SCN race 4 between resistant and susceptible reactions by using the Affymetrix soybean Genome GeneChip. Comparative analyses indicated that 21 common genes changed signiifcantly in the resistant group, of which 16 increased and 5 decreased. However, 12 common genes changed signiifcantly in the susceptible group, of which 9 increased and 3 decreased. Additionally, 27 genes were found in common between resistant and susceptible reactions. The 21 signiifcantly changed genes in resistant reaction were associated with disease and defense, cell structure, transcription, metabolism, and signal transduction. The fold induction of 4 from the 21 genes was conifrmed by quantitative RT-PCR （qRT-PCR） analysis. Moreover, the gene ontology （GO） enrichment analyses demonstrated the serine family amino acid metabolic process and arginine metabolic process may play important roles in SCN resistance. This study provided a new insight on the genetic basis of soybean resistance to SCN race 4, and the identiifed resistant or resistant-related genes are potentially useful for SCN-resistance breeding in soybean.

关键词： soybean soybean cyst nematode Affymetrix soybean Genome GeneChip gene ontology

Identification of Endophytic Bacillus BHL3501 from Wild soybean and Its Inhibitory Effect against soybean cyst nematode (Heterodera glycines Ichinohe)

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Plant Diseases and Pests 2011年第5期2卷 52-54,58页

作者：朱英波史凤玉李建英龙茹武云鹏乔亚科河北科技师范学院生命科技学院河北秦皇岛066600 黑龙江农业科学院大庆分院黑龙江大庆163316

[ Objective] The paper was to identify endophytic bacillus BHL3501 from wild soybean and its inhibitory effect against soybean cyst nematode. [ Meth- od] Through morphological characteristic, physiological and biochemical characteristics and 16S rDNA analysis, a strain of endophytic bacterium BHL3501 with ne- maticidal activity isolated and screened from the root of wild soybean was identified, the effects of BHL3501 metabolite solutions with different dilution multiples on egg hatching and activity of 2rd instar juvenile of soybean cyst nematode were also studied. [ Result ] BHL3501 strain was preliminarily identified to be Bacillus sp.. The relative inhibitory rate of its original fermentation broth on egg hatching of soybean cyst nematode after 24 h was 98.3% ; the relative inhibitory rate under 10- time diluted solution treatment was 79.5%, which had significant difference with sterile water control. The corrected mortality rate of 24 instar juvenile was 93.1% after treated by original fermentation broth for 24 h, the treatments of all diluted solutions had significant difference with sterile water control. [ Conclusion ] BHL3501 metabolites had strong inhibitory effect against egg hatching of soybean cyst nematode, which also had strong toxic effect on its 2nd instar juvenile.

关键词： Wild soybean soybean cyst nematode Biological control Bacillus sp. 16S rDNA China

Klebiella penumoniae SnebYK Induced System Resistance in soybean Against Drought and soybean cyst nematode

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Klebiella penumoniae SnebYK Induced System Resistance in Soy...

The prevalence of deleterious mutations during the domestication and improvement of soybean

中国植物病理学会2016年学术年会

作者： LIU Dan ZHAO Jing WANG Yuan-yuan ZHU Xiao-feng LIU Xiao-yu CHEN Li-jie DUAN Yu-xi Nematology Institute of Northern China College Plant ProtectionShenyang Agricultural University

soybean（Glycine max） is an important economic crop around the world due to its nutritive and commercial *** the one hand,soybean provides a large source of protein and *** the other hand,it can be ideal material to produce *** the field conditions,soybean is subjected to various biotic and abiotic stresses,such as drought and soybean cyst *** stresses will interfere with plant growth,result in a severe diminished output on soybean,and therefore its prevention is *** addition,Klebiella *** been reported to can induce system resistance against biotic and abiotic stresses in some *** aim of this research was to verify *** strain SnebYK can elicit induced system resistance of soybean against drought and soybean cyst *** drought stress,relative water content,growth parameter,and gas exchange of SnebYK-treated and untreated soybean were ***-root system was used to study SnebYK inducing system resistance against soybean cyst *** results showed that soybean treated by SnebYK in drought showed higher relative water content（4.75%）,root-top ratio（16.38%）,assimilation rate（134.06%） and water use efficiency（92.70%） than untreated *** SnebYK caused 67.05%reduction in total soybean cyst nematode ***,*** SnebYK are potential to protect soybean from drought and soybean cyst nematode in an environmentally friendly way,and the biochemical and molecular mechanism of them is worth studying.

关键词： Klebiella penumoniae soybean Induced system resistance Drought soybean cyst nematode

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The Crop Journal 2023年第2期11卷 523-530页

作者： Shichao Sun Yumin Wang He Wei David E.Hufnagel Ya Wang Shiyu Guo Yinghui Li Li Wang Li-juan Qiu Shenzhen Branch Guangdong Laboratory for Lingnan Modern Agriculture/Genome Analysis Laboratory of the Ministry of Agriculture/Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural SciencesShenzhen 518000GuangdongChina soybean Research Institute Jilin Academy of Agricultural SciencesChangchun 130033JilinChina Henan Academy of Crops Molecular Breeding Henan Academy of Agricultural Sciences/National Centre for Plant BreedingZhengzhou 450002HenanChina Virus and Prion Research Unit National Animal Disease CenterThe Agricultural Research Service(ARS)of the United States Department of Agriculture(USDA)AmesIA 50010USA The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/Key Laboratory of Crop Gene Resource and Germplasm Enhancement(MOA)/Key Laboratory of soybean Biology(Beijing)(MOA) Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Kunpeng Institute of Modern Agriculture at Foshan Foshan 528200GuangdongChina

soybean(Glycine max L.)is a protein and oil crop grown *** fitness may be reduced by deleterious mutations,whose identification and purging is desirable for crop *** the published whole-genome re-sequenced data of 2214 soybean accessions,including 221 wild soybean,1132 landrace cultivars and 861 improved soybean lines,we identified 115,275 deleterious single-nucleotide polymorphisms(SNPs).Numbers of deleterious alleles increased from wild soybeans to landraces and decreased from landraces to modern improved *** in selective-sweep regions showed fewer deleterious mutations than the remaining *** mutations explained 4.3%-48%more phenotypic variation than randomly selected SNPs for resistance to soybean cyst nematode race 2(SCN2),soybean cyst nematode race 3(SCN3)and soybean mosaic virus race 3(SMV3).These findings illustrate how mutation load has shifted during soybean domestication,expansion and improvement and provide candidate sites for breeding out deleterious mutations in soybean by genome editing and/or conventional breeding focused on the selection of progeny with fewer deleterious alleles.

关键词： Deleterious mutations Domestication cost Expansion load BAD_Mutations soybean cyst nematode

Functional response of the fungus Hirsutella rhossiliensis to the nematode, Heterodera glycines

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Science China(Life Sciences) 2015年第7期58卷 704-712页

作者： SHU Chi LAI YiLing YANG EnCe CHEN SenYu XIANG MeiChun LIU XingZhong State Key Laboratory of Mycology Institute of Microbiology Chinese Academy of Sciences University of Chinese Academy of Sciences Southern Research and Outreach Center University of Minnesota

Functional response is a key index in determining the population fluctuation in predation. However, the lack of operable research system limits the studies on functional response of fungal predators. Hirsutella rhossiliensis is a dominant parasite of the soybean cyst nematode, Heterodera glycines. In a soil microcosm bioassay, we determined fungal biomass at different days within 21 days after inoculation, and parasitism rate of H. glycines by the fungus was determined. The functional response of H. rhossiliensis to H. glycines was established and found to be Holling＇s type Ill, which was influenced by mycelial densities. Meanwhile, we conducted anti-fungal analysis of metabolic fractions extracted from H. rhossiliensis to explain the potential mechanism of the intraspecific competition illustrated by functional response. The result of anti-fungal experiments indicated that the fungal predators had more complicated interaction at population level than expected, which might be regulated by self-inhibition metabolite（s）. This study was the first functional response study of fungal predators in microcosm. With the in- creasing recognition of emerging fungal threats to animal, plant, and ecosystem health, the methodologies and hypotheses proposed in this study might inspire further research in fungal ecology.

关键词： fungal predator predation index self-inhibition composition soybean cyst nematode

Current Research Status of Heterodera glycines Resistance and Its Implication on soybean Breeding

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Engineering 2018年第4期4卷 534-541页

作者： Guiping Yan Richard Baidoo Department of Plant Pathology North Dakota State University Fargo ND 58108 USA

Heterodera glycines （i.e., soybean cyst nematode, SCN） is the most damaging nematode pest affecting soybean crop worldwide. This nematode is managed by means of crop rotation with selected resistant sources. With increasing reports of virulent SCN populations that are able to break the resistance within commonly used sources, there is an increasing need to find new sources of resistance or to broaden the resistance background. This review summarizes recent findings about the genes controlling SCN resis- tance in soybean, and about how these genes interact to confer resistance against SCN in soybean. It also provides an update on molecular mapping and molecular markers that can be used for the mass selection and differentiation of different resistance lines and cultivars in order to expedite conventional breeding programs. In-depth knowledge of SCN parasitism proteins and soybean resistance responses to the pathogen is critical for the diversification of resistant sources through gene modification, gene stacking, or incorporation of novel sources of resistance through backcrossing or genetic engineering.

关键词： soybean cyst nematode Heterodera glycines Resistance Molecular breeding

The Heterodera glycines effector Hg16B09 suppresses plant innate immunity

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The Heterodera glycines effector Hg16B09 suppresses plant in...

大豆孢囊线虫抗性相关基因的比较作图(英文)

中国植物病理学会2021年学术年会

作者：王宇 YOU Jia CHEN Aoshuang XU Lijian 胡岩峰 College of Modern Agriculture and Ecological Environment Heilongjiang University Northeast Institute of Geography and Agroecology Chinese Academy of Sciences Institute of Pratacultural Science Heilongjiang Academy of Agricultural Science

The soybean cyst nematode（SCN）,Heterodera glycines is one of the most destructive pathogens of soybean *** establish specific interactions with its hosts by using stylet to secrete effectors into host *** secreted by SCN,effectors perform their biological functions through manipulating important physiological metabolism pathways and defense responses in host *** each of these effectors to elucidate the underlying mechanistic data for SCN parasitism will aid developing novel strategies for SCN *** previously provided evidences that the effector Hgl6 B09 plays an important role in the parasitic process of *** this study,we use Nicotiana benthamiana to transiently express recombinant Hgl6 B09 plasmid,and immune elicitors which can trigger PTI and ETI defense ***,the effects of Hg 16 B09 on PTI and ETI suppression were investigated by determining the ROS burst,hypersensitive response（HR）,and the expression of defense marker *** expression of Hgl6 B09 in *** leaves suppressed significantly the burst of hydrogen peroxide and the expression levels of defense-related genes（PT15,WRKY22-A,WRKY22-B,and ACRE31） triggered by *** also found that Hgl6 B09 could also inhibit HR induced by Pseudomonas syringae DC 3000 and an avirulent effector ***-PCR analysis further showed that Hgl6 B09 caused significant down-regulation of defense genes（PR1 a,PR2,PI1,and WRKY51） in salicylic acid signaling during Pst DC 3000 *** results suggested that Hgl6 B09 acts as PTI and ETI suppressors during SCN-host interactions.

关键词： soybean soybean cyst nematode Effector PAMP-triggered immunity(PTI) Effectors-triggered immunity(ETI)

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大豆孢囊线虫抗性相关基因的比较作图(英文)