A fragment of a 70-kDa Heterodera glycines heat shock protein(HgHSP70)interacts with soybean cyst nematode-resistant GmSHMT08

作     者：LIU Zhi ZHANG Liu-ping ZHAO Jie JIAN Jin-zhuo PENG Huan HUANG Wen-kun KONG Ling-an PENG De-liang LIU Shi-ming LIU Zhi;ZHANG Liu-ping;ZHAO Jie;JIAN Jin-zhuo;PENG Huan;HUANG Wen-kun;KONG Ling-an;PENG De-liang;LIU Shi-ming

作者机构：State Key Laboratory for Biology of Plant Diseases and Insect PestsInstitute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China MARA–CABI Joint Laboratory for Bio-safetyInstitute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2022年第21卷第10期

页      面：2973-2983页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported by the National Natural Science Foundation of China(31972248) the Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences,China(ASTIP-02IPP-04) 

主　　题：soybean cyst nematode Heterodera glycines GmSHMT08 HgHSP70 interaction resistance 

摘      要：Soybean cyst nematode(SCN)Heterodera glycines is considered as the major constraint to soybean *** SHMT08 at Rhg4 locus on chromosome 08,encoding a serine hydroxylmethyltransferase,is a major gene underlying resistance against *** in Peking-type ***,the molecular mechanism underpinning this resistance is less well characterized,and whether Gm SHMT08 could interact with proteins in *** remains *** this study,yeast two-hybrid screening was conducted using Gm SHMT08 as a bait protein,and a fragment of a 70-kDa heat shock protein(Hg HSP70)was screened from *** that exhibited interaction with Gm *** interaction was verified by both GST pull-down and bimolecular fluorescence complementation *** finding reveals Hg HSP70 could be applied as a potential candidate gene for further exploring the mechanism on Gm SHMT08-mediated resistance against SCN ***.