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Natural Science 2013年第5期5卷 599-607页

作者： Kim Z. Gamburg Viktor K. Voinikov Siberian Institute of Plant Physiology and Biochemistry Siberian Branch of the Russian Academy of Sciences Irkutsk Russia

Adventive regenerants of Populus × berolinensis Dipp. were obtained on 1/2 MS salts with sucrose, vitamins, BA, TDZ and NAA using stem, petiole and leaf explants. They differed from each other in plantlet dimensions. More than 50 clones were produced from initial regenerants by excision and rooting of apexes and lateral shoots. Differences in stem length and thickness were observed between 200 field plants produced from in vitro plantlets. Differences in stem length were conditioned mainly due to different number of internodes and not by internodes’ lengths. Plants grown from cuttings excised from highest and smallest plants retained the abilities of mother plants to grow with different rates. It is concluded that somaclonal variability can be used for selection of fast growing poplar plants which are necessary for industrial plantations. These plants can be used for this purpose without the limitations existing for transgenic plants.

关键词： somaclonal Variations Adventive Regenerants Poplar Populus x Berolinensis Dipp. Propagation in Vitro Fast Growing Plants

Functional Analysis for Rolling Leaf of somaclonal Mutants in Rice (Oryza sativa L.)

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American Journal of Plant Sciences 2011年第1期2卷 56-62页

作者： Young-Hie Park Hyun-Suk Lee Gi-Hwan Yi Jae-Keun Sohn Kyung-Min Kim 不详

This study was carried out to facilitate the functional analysis of rice genes. Some 297 insertion plants (1.7%) of the entire lines with the endogenous retrotransposon Tos17 were produced. Phenotypes of these plants in the S2 genera-tion were observed in the field according to different leaf types. Rolling leaf mutants showed thinner sclerenchyma-tous cells, defective arrangement of vascular bundles, and well-formed bulliform cells as compared to the parental cultivar. Two new copies of Tos17 were detected in the rolling leaf type. In the new leaf type, the copy number and activation of Tos12, 15 did not appear as ‘Ilpum’. Flanking sequence tag (FST) analysis of Tos17 in the rolling leaf mutant indicated that new copies of Tos17 were transposed on chromosomes 11 and 12. Annotated homologues of the tagging genes on chromosome 11 were arabinoxylan rabinofuranohydrolase isoenzyme AXAH-I and II. The tagging gene in chromosome 12 was highly correlated with 6 kinds of genes including a transcript regulated factor and a rough sheath 2-like protein. This rolling leaf and flanking sequence data will stimulate the functional analysis of rice genes.

关键词： Tissue Culture somaclonal Variation Mutant Opaque Endosperm Tos Element

RITSD Analysis of the Agronomic Traits of somaclonal in Rice

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Agricultural Science & Technology 2010年第1期11卷 36-41页

作者：谢戎杨正林刘成元李永洪桑贤春何光华四川省农科院水稻高粱研究所四川泸州646000 西南大学水稻研究所重庆400716

[Objective]The aim was to study the sensitive response characteristics of the main agronomic traits of somaclonal and the variation of sensitive response of the lines of somaclonal to sowing date. [Method]The RITSD changing of agronomic traits was studied by using 24 rice somaclonal and its donor parent under two sowing dates. [Result]The average values of RITSD were higher （over one） for the traits of pant height （PH）,density of spikelets （DS）,filled spikelets per panicle （FSP）,spikelets per panicle （SP） and grain weight per plant （GWP）,but lower for days of sowing to heading （DSH）,effective panicles （EP）,panicle length （PL）,seed setting rate （SS） and 1 000-grain weight （1 000-GW）. These showed that PH,DS,FSP,SP and GWP were easily affected by sowing date and tended to higher with the later sowing; the RITSD of somaclonal changed with the agronomic traits. The frequency of RITSD＇ variation of DSH and PH was higher than that of PL. The RITSD＇ value of EP,PL,DS,FSP,SP and SS became higher,and higher or lower for DSH and GWP in different somaclonal; compared with the donor,20 of 24 was significantly different,and the frequency of RITSD variation was over 83. 3%,however,RITSD of the most lines （75%） changed only for 1-2 characters; cluster analysis showed that the RITSD of agronomic traits belonged to different combinations of somaclonal in rice. [Conclusion]the study had provided a theoretical basis for the screening and using of the somaclonal.

关键词： Rice (Oryza sativa L.) somaclonal Respondence Index of Trait to Sowing Date (RITSD) Agronomic traits Variation

HMW-GS variation of somaclones and its variants in Triticum aestivum

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Chinese Science Bulletin 1996年第4期41卷 330-334页

作者：张怀刚陈集贤赵绪兰胡含 Northwest Plateau Institute of Biology Chinese Academy of Sciences Xining 810001 China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics Chinese Academy of Sciences Beijing 100101 China

Research on somacolonal variation in hexaploid wheat (Triticum aestivum L.) has been conducted a lot and morphological, cytological, and biochemical variations have been observed. Evidence for gene mutations has been obtained through the analysis of gliadin and glutenin in wheat grain, but loci of mutations have not been located. The aims of this study are: firstly, to detect mutation loci of specific gene (Glu-1) to provide exact evi-dence for gene mutation by analyzing changes of high-molecular-weight glutenin

关键词： wheat somaclonal variation HMW-GS variant.

Restorable variation of cytoplasmic type of male sterile line in rice by in vitro culture

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Chinese Science Bulletin 2000年第24期45卷 2246-2250页

作者： Dinghou Ling Zhenrong Ma Chinese Acad Sci S China Inst Bot Guangzhou 510650 Peoples R China

Rice male sterile (MS) lines, IR66707A and IR69700A, which possess the cytoplasm of Oryza perennis and O. glumaepatula respectively, belong to the cytoplasmic type. Their sterility could be maintained but not be restored. By somatic cell culture of these two MS lines, 47 somaclones with 465 R1 plants were obtained. All of the 465 R1 plants were sterile in the spring season in Guangzhou. According to the expression of the R1 plants and the level of similarity to their donor parents, they could be divided into three types. The plants of type I were male sterile. The sterility of some somaclones of this type could be restored by the test crossing varieties or alternated to fertile by changes of some environmental conditions. The hybrid F1 of test cross from the MS somaclones in type I was fertile while the hybrid F1 from the donor MS lines was still sterile. The R1 plants of type Ⅱ were similar to the donor parents and also male sterile. The hybrid F1 from all of the plants of type Ⅱ crossed to test

关键词： somaclonal variation cytoplasm-type of MS restorable variation of male sterile rice.

Studies on Immature Embryo Culture In vitro in Wheat:Genetic stability analysis of somatic variation in main agronomic characteristics

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Journal of Northeast Agricultural University(English Edition) 1995年第1期2卷 11-16页

作者： Li Wenxiong Zeng Hanbing and Hu Shanglian(Northeast Agriculture University Harbin 150030, P R C)

The genetic stability of variations of main agronomic characteristics in progenies from the regenerated plants of immature embryo culture in vitro was studied. The results showed that the variations of earliness in maturity, high 1000-grain weight, plant height and grain weight per spike were heritable and tended to be stable in IE3 and completely stable in IE4 The wide variation of main agronomic characteristics induced by embryo culture in vitro provided the probability of selection in wheat improvement. Some somaclonal lines with useful variations could be used directly in wheat production.

关键词： agronomic characteristics embryo culture somaclonal variation,gen etic stability wheat