Restorable variation of cytoplasmic type of male sterile line in rice by in vitro culture
Restorable variation of cytoplasmic type of male sterile line in rice by in vitro culture作者机构:Chinese Acad Sci S China Inst Bot Guangzhou 510650 Peoples R China
出 版 物:《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)
年 卷 期:2000年第45卷第24期
页 面:2246-2250页
核心收录:
基 金:Rockefeller Foundation: 1994-001-02402 Natural Science Foundation of Guangdong Province: 990771
主 题:somaclonal variation cytoplasm-type of MS restorable variation of male sterile rice.
摘 要:Rice male sterile (MS) lines, IR66707A and IR69700A, which possess the cytoplasm of Oryza perennis and O. glumaepatula respectively, belong to the cytoplasmic type. Their sterility could be maintained but not be restored. By somatic cell culture of these two MS lines, 47 somaclones with 465 R1 plants were obtained. All of the 465 R1 plants were sterile in the spring season in Guangzhou. According to the expression of the R1 plants and the level of similarity to their donor parents, they could be divided into three types. The plants of type I were male sterile. The sterility of some somaclones of this type could be restored by the test crossing varieties or alternated to fertile by changes of some environmental conditions. The hybrid F1 of test cross from the MS somaclones in type I was fertile while the hybrid F1 from the donor MS lines was still sterile. The R1 plants of type Ⅱ were similar to the donor parents and also male sterile. The hybrid F1 from all of the plants of type Ⅱ crossed to test