检索结果-南通市图书馆

small interfering rna targeting PGC-1α inhibits VEGF expression and tube formation in human retinal vascular endothelial cells

引用

International Journal of Ophthalmology(English edition) 2015年第5期8卷 877-883页

作者： Jian Jiang Lu Zhang Xiao-Bo Xia Department of Ophthalmology Xiangya HospitalCentral South University

AIMTo determine whether small interfering rna (sirna) of PGC-1α could inhibit vascular endothelial growth factor (VEGF) expression and tube formation in human retinal vascular endothelial cells (hRVECs).METHODShRVECs transfected with peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) sirna were incubated for 24h and then placed into a normoxic (20%, O2) or hypoxic (1%, O2) environment for another 16h. PGC-1α mrna and protein levels were detected by real-time PCR and Western blot. VEGF mrna and protein levels were detected by real-time PCR and ELISA. Cell proliferation was evaluated by BrdU incorporation assay. Forty-eight hours after sirna transfection, hRVECs were planted into Matrigel-coated plates and cultured under normoxic (20%, O2) or hypoxic (1%, O2) conditions for another 48h. The tube formation of hRVECs was observed under an optical microscope and quantified by counting the number of branch points and calculating the total tube ***-1α mrna and protein levels were significantly reduced by PGC-1α sirna, and VEGF mrna and protein levels also decreased significantly. The percentage of BrdU-labeled cells in siPGC-1α groups were significantly decreased compared with control sirna groups under normoxia and hypoxia in cell proliferation assay. In the tube formation assay, PGC-1α sirna treated cells formed significantly fewer *** PGC-1α expression can inhibit VEGF expression in hRVECs and inhibit their ability to form tubes under both normoxic and hypoxic conditions.

关键词： peroxisome proliferator-activated receptor-γ coactivator-1α vascular endothelial growth factor small interfering rna retinal vascular endothelial cell tube formation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

small interfering rna-mediated inhibition of hepatitis G virus gene expression in human hepatoma cell Huh-7

引用

Science China(Life Sciences) 2005年第1期48卷 61-69页

作者： CAO Mingmei REN Hao ZHAO Ping PAN Wei ZHAO Lanjuan QI Zhongtian Department of Microbiology Second Military Medical UniversityShanghai200433China

The rna interference(rnai)phenomenon is a recently observed process in which the introduction of a double-stranded,small interfering rna(sirna)into a cell causes the specific degradation of a homologous single-stranded *** represents an exciting new technology that could have therapeutic applications for the treatment of viral *** hepatitis G virus(HGV)genome is a positive-sense single-stranded rna,the replication of HGV does not lead to an integrated DNA genome,suggesting a particularly attractive target for rnai study that could eliminate viral rna from infected *** eukaryotic expression vector *** containing the cDNA sequences of the entire HGV structural genes and hygromycin resistance gene downstream from the encephalomyocarditis virus(ECMV)internal ribosome entry site(IRES)was constructed and transfected into human hepatoma cell *** modified cleavage products of the structural proteins of HGV expressed in hygromycin-resistant cell line Huh-7-EH were confirmed by RT-PCR and Western blot *** specific HGV E2 sirnas(1-E2sirna,2-E2 sirna)synthesized with T7 rna polymerase by transcription in vitro were transfected into the Huh-7-EH *** the analyses of Western blot and the formation of hygromycin-resistant colonies,the inhibitions of expression of HGV structural protein by two HGV E2sirnas were detected and found lasting at least one *** inhibition of 2-E2 sirna was stronger and only 1%of the cells treated with 2-E2 sirna formed hygromycin-resistant *** results support that specific HGV 2-E2 sirnas mediate the degradation of mrna spanning from HGV structural gene cDNA to hygromycin resistance gene in a majority of *** conclusion,the Huh-7-EH cells expressing HGV structural proteins stably can be used as a cell model for studying the replication of HGV and rnai and the enlargement of rnai may exist,in mammalian cells.

关键词： rna interference small interfering rna hepatitis G virus structural gene cell model.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Suppression of Type 1 Insulin-like Growth Factor Receptor Expression by small interfering rna Inhibits A549 Human Lung Cancer Cell Invasion in vitro and Metastasis in Xenograft Nude Mice

引用

Acta Biochimica et Biophysica Sinica 2007年第2期39卷 137-147页

作者： Jianfang QIAN~1 Aiqiang DONG~1 Minjian KONG~1 Zhiyuan MA~2 Junqiang FAN~1 and Guanyu JIANG~3 ~1Department of Cardiothoracic Surgery,Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China ~2Department of Cardiovascular Surgery,Shanghai Jiaotong University Affiliated First People's Hospital,Shanghai 200080,China ~3Department of Emergency,Second Affiliated Hospital School of Medicine,Zhejiang University,Hangzhou 310009,China Department of Cardiothoracic Surgery Second Affiliated Hospital School of Medicine Zhejiang UniversityHangzhou 310009 China Department of Cardiovascular Surgery Shanghai Jiaotong University Affiliated First People's HospitalShanghai 200080 China Department of Emergency Second Affiliated Hospital School of Medicine Zhejiang UniversityHangzhou 310009 China

Cancer invasion and metastasis, involving a variety of pathological processes andcytophysiological changes,contribute to the high mortality of lung *** type 1 insulin-like growthfactor receptor (IGF-1R),associated with cancer progression and invasion,is a potential anti-invasion andanti-metastasis target in lung *** inhibit the invasive properties of lung cancer cells,we successfullydown-regulated IGF-1R gene expression in A549 human lung cancer cells by small interfering rna (sirna)technology,and evaluated its effects on invasion-related gene expression,tumor cell in vitro invasion,andmetastasis in xenograft nude mice.A549 cells transfected with a plasmid expressing hairpin sirna forIGF-1R showed a significantly decreased IGF-1R expression at the mrna level as well as the *** biological assays,transfected A549 cells showed a significant reduction of cell-matrix adhesion,migration and *** with these results,we found that down-regulation of IGR-1Rconcomitantly accompanied by a large reduction in invasion-related gene expressions,including MMP-2,MMP-9,u-PA,and IGF-1R specific downstream *** tail vein injections of plasmid expressinghairpin sirna for IGF- 1R significantly inhibited the formation of lung metastases in nude *** resultsshowed the therapeutic potential of sirna as a method for gene therapy in inhibiting lung cancer invasionand metastasis.

关键词： lung cancer invasion metastasis type 1 insulin-like growth factor receptor small interfering rna

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

small interfering rna targeting of keratin 17 reduces inflammation in imiquimod-induced psoriasis-like dermatitis

引用

Chinese Medical Journal 2020年第24期133卷 2910-2918页

作者： Chun-Ying Xiao Zhen-Lai Zhu Chen Zhang Meng Fu Hong-Jiang Qiao Gang Wang Er-Le Dang Department of Dermatology Xijing HospitalFourth Military Medical UniversityXi’anShaanxi 710032China

Background:Psoriasis is a common chronic inflammatory skin disease with 2%to 3%prevalence worldwide and a heavy social-psychological burden for patients and their *** the exact pathogenesis of psoriasis is still unknown,the current treatment is far from ***,there is an urgent need to find a more effective therapy for this *** 17(K17),a type I intermediate filament,is overexpressed in the psoriatic epidermis and plays a critical pathogenic role by stimulating T cells in ***,we hypothesized that inhibiting K17 may be a potential therapeutic approach for *** study aimed to investigate the therapeutic effect of K17-specific small interfering rna(sirna)on mice with imiquimod(IMQ)-induced psoriasis-like ***:Eight-week-old female BALB/c mice were administered a 5%IMQ cream on both ears to produce psoriatic *** day 3,K17 sirna was mixed with an emulsion matrix and applied topically to the left ears of the mice after IMQ application every day for 7 *** right ears of the mice were treated in parallel with negative control(NC)*** was evaluated by gross ear thickness,histopathology,the infiltration of inflammatory cells(CD3+T cells and neutrophils)using immunofluorescence,and the expression of cytokine production using real-time quantitative polymerase chain *** obtained data were statistically evaluated by unpaired t-tests and a one-way analysis of ***:The severity of IMQ-induced dermatitis on K17 sirna-treated mice ears was significantly lower than that on NC sirna-treated mice ears,as evidenced by the alleviated ear inflammation phenotype,including decreased ear thickness,infiltration of inflammatory cells(CD3+T cells and neutrophils),and inflammatory cytokine/chemokine expression levels(interleukin 17[IL-17],IL-22,IL-23,C-X-C motif chemokine ligand 1,and C-C motif chemokine ligand 20)(Prna groups).Compared to the NC sirna

关键词： Psoriasis Keratin 17 small interfering rna Imiquimod Inflammation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

small interfering rna of cyclooxygenase-2 induces growth inhibition and apoptosis independently of Bcl-2 in human myeloma RPMI8226 cells

引用

Acta Pharmacologica Sinica 2007年第7期28卷 1031-1036页

作者： Qiu-bai LI Zhi-chao CHEN Yong YOU Ping ZOU Department of Hematology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China Department of Hematology Union Hospital Tongji Medical College HuazhongUniversity of Science and Technology Wuhan 430022 China. doctorlee007@***.

Aim:To investigate the effects of small interfering rna of cyclooxygenase-2(COX-2) on the proliferation and apoptosis of human multiple myeloma RPMI8226cells and its relation with the Bcl-2 family in ***:Transcription andexpression of COX-2 in human myeloma RPMI8226 cells were checked by RT-PCRand Western blot analysis,*** COX-2 sirna fragment targetingexon 5 of COX-2 gene was transfected into the cells with the Amaxa *** inhibition of cell growth was detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium (MTT) *** was estimatedby Annexin-V/propidium iodide double-labeled cytometry and confirmed by termi-nal deoxynucleotidyl transferase-mediated dUTP nick end-labeling ***-2and Bax expression was evaluated by Western blot ***:The COX-2sirna fragment could be successfully transfected into RPMI8226 cells,whichresulted in the significant inhibition of transcription and expression of COX-2 inthe myeloma *** of the transfected cells was inhibited andapoptosis was induced (6.52%±0.32%,12.53%±2.52%,24.39%±3.51% and36.48%±4.96% for 0,12,24,and 48 h,respectively) in a time-dependent manner(Prna transfectioncan suppress COX-2 expression in human myeloma RPMI8226 cells,which leadsto growth inhibition and apoptosis independent of Bcl-2.

关键词： RPMI8226 small interfering rna apoptosis proliferation

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

small interfering rna-mediated knockdown of Notch1 in lung T cells of asthmatic mice affects T cell differentiation

引用

Chinese Medical Journal 2009年第21期122卷 2647-2651页

作者： GUO Xue-jun ZHOU Min REN Lian-ping YANG Min HUANG Shao-guang XU Wei-guo Department of Respiratory Medicine Ruijin Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200025 China Department of Respiratory Medicine Xinhua Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200092 China Department of Respiratory Medicine Shanghai Third Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200092 China

Background The immunologic response to allergens mediated by T lymphocytes is an incipient key element in the pathogenesis of asthma, and Thl/Th2 balance is regarded as the core of asthma pathogenesis. Notch is a single-pass transmembrane receptor protein that regulates differentiation, proliferation and apoptosis in a broad range of cells. It is considered that the Notch signal pathway works in every stage of T cell development and differentiation. Whether the pathway of asthma pathogenesis is related to Notch1 remains unknown. This study is aimed to investigate whether the pathway of asthma pathogenesis is related to Notch1 by examining the effect of knockdown of the Notch1 gene by small interfering rna on T cell differentiation. Methods An OVA-induced asthma mouse model was established. The expression of Notch1 in the tissue and T cells of the lung from asthmatic mice was detected by RT-PCR and Western blotting. The expression of Notch1 and cytokine interleukin （IL）-4 and interferon （IFN）-γ in activated lung T cells was detected by RT-PCR and enzyme-linked immunosorbent assay after blocking Notch1 by small interfering rna. Results The mrna and protein expression of Notch1 increased significantly both in the lung tissue and lung T cells of asthmatic mice （both P 〈0.05）. IL-4 decreased and IFN-y increased significantly in active lung T cells after Notch1 was blocked by Notchl-specific small interfering rna （IL-4：（2.51±0.51） pg/ml vs 0.64±0.27） pg/ml protein; IFN-γ：（21.72±4.24） pg/ml vs （39.79±4.09） pg/ml protein, P 〈0.05）. Conclusion This study demonstrated that the Notch1 signal might play a role in the pathogenesis of asthma by its involvement in Thl/Th2 differentiation.

关键词： asthma Notchl receptor T-lymphocytes small interfering rna

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Progress of Targeting Transforming Growth Factor-β1 small interfering rna in Liver Fibrosis

引用

Chinese Medical Sciences Journal 2014年第4期29卷 231-235页

作者： Xuan Zhou Xue-feng Yang Department of Gastroenterology Affiliated Nanhua Hospital University of South China

Liver fibrosis is a common pathological consequence of a variety of chronic stimuli, including viral, autoimmune, drug-induced, cholestatic and metabolic diseases. Fibrosis is driven by a dynamic process involving increased synthesis of matrix components and a failure of physiological mechanisms of matrix turnover. Activation of hepatic stellate cells(HSCs) remains a central event in fibrosis. HSCs are the main source of extracellular matrix(ECM). Transforming growth factor-beta(TGF-β), which is the fibrogenic master cytokine, can induce the activation of HSCs to produce a large amount of ECM, and is capable of inducing apoptosis of liver cells. rna interference(rnai) is a novel gene disruption technology. Studies have shown that small interfering rna(si rna) targeting TGF-β1 may inhibit the activation and proliferation of HSCs, suppress ECM synthesis and block liver fibrosis. TGF-β1 si rna-mediated gene silencing therapy provides a new avenue for liver fibrosis. This review summarizes recent progresses in research on HSCs, TGF-β1 and TGF-β1 si rna in liver fibrosis.

关键词： liver fibrosis hepatic stellate cells transforming growth factor-β small interfering rna

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

BcI-XL small interfering rna Sensitizes Cisplatin-resistant Human Lung Adenocarcinoma Cells

引用

Acta Biochimica et Biophysica Sinica 2007年第5期 344-350页

作者： Xiaoyong LEI~*, Zexiang HUANG, Miao ZHONG, Bingyang ZHU, Shengsong TANG, and Duanfang LIAO Institute of Pharmacy and Pharmacology University of South China, Hengyang 421001, China

Bcl-ⅩL is overexpressed in a variety of human tumors and is involved in tumorigenesis and chemoresistance. This study investigated the inhibitory effect of the hairpin Bcl-ⅩL small interfering rna (sirna) on the expression of the Bcl-ⅩL gene in the cisplatin (DDP)-resistant human lung adenocarcinoma cell line A549/DDR and the effect of Bcl-ⅩL sirna on drug sensitization in A549/DDP cells. Bcl-ⅩL sirna and negative sirna plasmids were constructed and stably transfected into A549/DDP cells. Reverse transcription-polymerase chain reaction and Western blot analysis were used to detect the target gene expression. Spontaneous apoptosis of cells was detected by acridine orange and ethidium bromide staining. Drug sensitivity of the cells to DDP was analyzed with dimethylthiazol-diphenyltetrazolium bromide (MTT) and flow cytometry. Expression levels of Bcl-ⅩL mrna and protein in sirna stable transfectants were clearly reduced as compared with negative sirna transfectants and untreated cells. MTT results indicated that Bcl-ⅩL transfectants had a higher cell inhibition rate than the negative vector or untreated cells after treatment with 0.2-200μg/ml DDP. Flow cytometry revealed increased apoptosis in BcI-XL sirna cells. After the addition of 20μg/ml DDR sirna targeting of the Bcl-ⅩL gene specifically down-regulated gene expression in A549/DDP cells, increased spontaneous apoptosis, and sensitized cells to DDP. The results showed that Bcl-ⅩL sirna contributed to an increase of DDP-induced cell death in non-small-cell lung cancer and sensitized cells to DDP, leading to increased the effectiveness of the drug in treating non-small-cell lung cancer.

关键词： apoptosis small interfering rna adenocarcinoma Bcl-XL A549/DDP

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

KNOCKDOWN OF SURVIVIN EXPRESSION BY small interfering rna SUPPRESSES PROLIFERATION OF TWO HUMAN CANCER CELL LINES

引用

Chinese Medical Sciences Journal 2006年第2期21卷 115-119页

作者： Hai-tao Guan Xing-huan Xue Xi-jing Wang Ang Li Zhao-yin Qin Department of Ontological Surgery the Second AffiliatextHospital Medical College of Xi＇an Jiaotong University Xi＇an 710004 Medical Research Center Affiliated Stomatological l-Iospital Medical College of Xi＇an Jiaotong University Xi＇an 710004 Department of General Surgery the Second Affiliat~iHospital Medical College of Xi＇an Jiaotong University Xi＇an 710004

Objective To construct an expression vector of small interfering rna （sirna） against survivin and observe its effects on survivin expression and proliferation of human pancreatic cancer cell line PC-2 and breast cancer cell line MCF-7. Methods Constructed an expression vector of sirna against survivin and transfected it into PC-2 and MCF-7 cells using lipofectamine^TM 2000. The expression of survivin was detected by semi-quanfifive RT-PCR and immunohistochemistry, and its effects on proliferation of PC-2 and MCF-7 cells were detected by MTT assay. Results The introduction of sequence-specific sirna could efficiently suppress survivin expression at both mrna and protein levels in the two cancer cell lines. In PC-2 cells, the expression inhibition rates were 81.25% at mrna level and 74.24% at protein level In MCF-7 cells, the expression inhibition rates were 64.91% at mrna level and 79. 72% at protein level The proliferation of PC-2 and MCF-7 cells was also suppressed, and24 and 48 hours after the cells were reseeded, the proliferation inhibition rates of PC-2 cells were 28. 00% and 33. 38%, and that of MCF-7 cells were 31.58% and 33.02%, respectively. Conclusions The expression vector of sirna against survivin can block survivin expression in PC-2 and MCF-7 cells efficiently and specifically. Down regulation of survivin expression can suppress proliferation of PC-2 and MCF-7 cells. Survivin rnai may have potential value in gene therapy of human cancers.

关键词： rna interference small interfering rna survivin pancreatic cancer breast cancer

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Impact of Bovine Skeletal Muscle Satellite Cell Differentiation by small interfering rna Targeting Myogenin Gene

引用

Journal of Northeast Agricultural University(English Edition) 2013年第2期20卷 32-37,F0003页

作者： Liu Cong-cong Zhao Dan-dan Tong Hui-li Ye Feng Yang Yue Li Shu-feng Jia Ming-yu Yan Yun-qin College of Life Sciences Northeast Agricultural University

To examine the effect of myogenin gene on the differentiation of bovine skeletal muscle satellite cell, we constructed small interfering rna plasmid vector to obtain myogenin knockdown bovine skeletal muscle cells, then used cell transfection, real time RCR and Western Blot to detect the influence of myogenin to cell differentiation. Results showed that the knockdown of myogenin significantly decreased its expression and other muscle-specific genes. Compared to the control, it could differentiate into few myotubes when challenged by low serum in the medium. These findings provided an important theoretical basis for further explore of the genetic mechanism in adult skeletal muscle, the remedy of muscle injuries and the cultivation of high-yield transgenic cattle.

关键词： myogenin small interfering rna adult bovine skeletal muscle satellite cell differentiation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：