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Bioinformatics-led design of single-chain antibody molecules targeting DNA sequences for retinoblastoma

International Journal of Ophthalmology(English edition) 2011年第1期4卷 8-13页

作者： Guo-Gang Shang, Jun Yun Department of Radiotherapy Zhengzhou People's Hospital Zhengzhou 450052 Henan Province China The Faculty of Biomedical Engineering of Zhengzhou University Zhengzhou 450001 Henan Province China Department of Vascular and Endocrine Surgery Xijing Hospital Fourth Military Medical University Xi'an 710032 Shaanxi Province China

The development of single-chain Fv antibody (scFv) by recombinant gene expression is an important milestone for cancer therapy. single-chain antibodies are reconstructed for cancer-targeted therapy to provide good penetration into tumor tissue and to improve their pharmacokinetics in vivo, offering a clinically valuable application. The relationship needs to be analyzed that there may be some variations between the structure and function of the fusion proteins, and the relationship between the structure and function of protein molecules was obtained through analyzing relevant literature at home and abroad as well as modeling analysis. Through our analysis of the interaction region between antibody and antigen, and of the binding sites for molecular conformation, it is clear that existing antibodies need to be modified at the DNA sequence level, enhancing the biological activity of the antibodies. Based on the view that bio-molecular computer models are closely integrated with biological experiments, a bio-molecular structure-activity relationship model can be established in terms of molecular conformation, physical and chemical properties and the biological activity of single-chain antibodies. Two enlightenments are obtained from our analysis. On one hand, the structure-activity relationship is clear for new immune molecules at the gene expression level. On the other hand, a single-chain antibody molecule can be designed and optimized for the cancer-oriented treatment. In this article, we provided the theoretical and experimental basis for the development of single-chain antibodies appropriate for retinoblastoma therapy.

关键词： single-chain antibody bioinformatics retinoblastoma structure-activity relationship

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Anti-amyloid beta single-chain Fv ameliorates behavioral impairment in Alzheimer's disease mice via adeno-associated virus delivery

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Neural Regeneration Research 2011年第2期6卷 96-100页

作者： Jiong Cai Yanwei Zhong Fang Li Shizhen Wang Department of Nuclear Medicine Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union MedicalCollege Beijing 100730 China Viral Hepa-titis Research Laboratory Institute of Infectious Diseases the 302 Hospital of Chinese PLA Beijing 100039 China

Intracranial delivery of human Fc-deleted antibody specific to amyloid-β peptide （Aβ, anti-Aβ single-chain Fv, scFv） via adeno-associated virus （AAV） inhibits amyloid deposition in transgenic mice. However, the effects of AAV-mediated Fc-deleted antibody on animal behavior remain unclear. In this study, the anti-Aβ scFv antibody gone, isolated from phage display, was fused to the 5＇ end of the scFv antibody gone for antibody secretion by 2 rounds of polymerase chain reaction amplification. The fused antibody cDNA was cloned into a pSNAV2 plasmid under the control of the cytomegalovirus promoter. The sequence verified expression vector pSNAV2/scFv was transferred to BHK-21 ceils, and stable transfected BHK-21/scFv cells were established by G418 selection and infected with the recombinant herpes simplex virus rHSV/repcap for AAV production. Recombinant AAV was injected into the left quadriceps femoris of PDAPP transgenic mice. After 3 months, Morris water-maze results confirmed significantly improved cognitive function in a mouse model of Alzheimer＇s disease. Key Words： Alzheimer＇s disease; adeno-associated virus; amyloid-β peptide; single-chain antibody; neurodegenerative diseases; neural regeneration

关键词： Alzheimer's disease adeno-associated virus amyloid-13 peptide single-chain antibody neurodegenerative diseases neural regeneration

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Generation of high affinity human single-hain antibody against PreSl of hepatitis B virus from immune phage-display antibody library

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Hepatobiliary & Pancreatic Diseases International 2004年第1期3卷 77-81页

作者： Zhi-Chao Zhang, Xue-Jun Hu and Qing Yang Dalian, China State Key Laboratory of Fine Chemicals, Dalian Uni- versity of Technology, Dalian 116012, China

BACKGROUND: A single-chain antibody ( ScFv) phage display library was created by cloning antigen-binding re- gions of VH (variable domain) and VL gene repertoires as fusion proteins with a minor coat protein of filamentous phage, from which high affinity completely humanized ScFv against PreS1 of hepatitis B virus could be screened and characterized. METHODS: A combinatorial library of phage-display hu- man ScFv genes, which were derived from peripheral blood lymphocytes immunized by peptide PreS1 in vitro, was constructed. The library contained 7 × 108 clones. RESULTS: After 3 rounds panning, a high affinity (K = 10-7-10-8 mol/L) ScFv specific to PreS1 was obtained. Sequence analysis showed that the VH belonged to the VH4 family and Vλ to Vλ4. CONCLUSIONS: The described ScFv may provide a more satisfactory therapy. This application further illustrates that the method of in vitro antigen stimulation is expeditious for the source of human immune antibody library.

关键词： hepatitis B virus PreS1 single-chain antibody immune antibody library panning

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