Anti-amyloid beta single-chain Fv ameliorates behavioral impairment in Alzheimer's disease mice via adeno-associated virus delivery

作     者：Jiong Cai Yanwei Zhong Fang Li Shizhen Wang 

作者机构：Department of Nuclear Medicine Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union MedicalCollege Beijing 100730 China Viral Hepa-titis Research Laboratory Institute of Infectious Diseases the 302 Hospital of Chinese PLA Beijing 100039 China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2011年第6卷第2期

页      面：96-100页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071006[理学-神经生物学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：the National Natural Science Foundation of China No. 30670741 

主　　题：Alzheimer's disease adeno-associated virus amyloid-13 peptide single-chain antibody neurodegenerative diseases neural regeneration 

摘      要：Intracranial delivery of human Fc-deleted antibody specific to amyloid-β peptide （Aβ, anti-Aβ single-chain Fv, scFv） via adeno-associated virus （AAV） inhibits amyloid deposition in transgenic mice. However, the effects of AAV-mediated Fc-deleted antibody on animal behavior remain unclear. In this study, the anti-Aβ scFv antibody gone, isolated from phage display, was fused to the 5＇ end of the scFv antibody gone for antibody secretion by 2 rounds of polymerase chain reaction amplification. The fused antibody cDNA was cloned into a pSNAV2 plasmid under the control of the cytomegalovirus promoter. The sequence verified expression vector pSNAV2/scFv was transferred to BHK-21 ceils, and stable transfected BHK-21/scFv cells were established by G418 selection and infected with the recombinant herpes simplex virus rHSV/repcap for AAV production. Recombinant AAV was injected into the left quadriceps femoris of PDAPP transgenic mice. After 3 months, Morris water-maze results confirmed significantly improved cognitive function in a mouse model of Alzheimer＇s disease. Key Words： Alzheimer＇s disease; adeno-associated virus; amyloid-β peptide; single-chain antibody; neurodegenerative diseases; neural regeneration