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Overexpression of a Potato Sucrose Synthase Gene in Cotton Accelerates Leaf Expansion, Reduces seed abortion, and Enhances Fiber Production

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Molecular Plant 2012年第2期5卷 430-441页

作者： Shou-Min Xu Elizabeth Brill Danny J. Llewellyn Robert T. Furbank Yong-Ling Ruan CSIRO Plant Industry GPO Box 1600 Canberra ACT 2601 Australia School of Environmental and Life Sciences University of Newcastle Newcastle NSW 2308 Australia

Sucrose synthase （Sus） is a key enzyme in the breakdown of sucrose and is considered a biochemical marker for sink strength, especially in crop species, based on mutational and gene suppression studies. It remains elusive, however, whether, or to what extent, increase in Sus activity may enhance sink development. We aimed to address this question by expressing a potato Sus gene in cotton where Sus expression has been previously shown to be critical for normal seed and fiber development. Segregation analyses at T1 generation followed by studies in homozygous progeny lines revealed that increased Sus activity in cotton （1） enhanced leaf expansion with the effect evident from young leaves emerging from shoot apex; （2） improved early seed development, which reduced seed abortion, hence enhanced seed set, and （3） promoted fiber elongation. In young leaves of Sus overexpressing lines, fructose concentrations were significantly increased whereas, in elongating fibers, both fructose and glucose levels were increased. Since hexoses contribute little to osmolality in leaves, in contrast to developing fibers, it is concluded that high Sus activity promotes leaf development independently of osmotic regulation, probably through sugar signaling. The analyses also showed that doubling the Sus activity in 0-d cotton seeds increased their fresh weight by about 30%. However, further increase in Sus activity did not lead to any further increase in seed weight, indicating an upper limit for the Sus overexpression effect. Finally, based on the observed additive effect on fiber yield from increased fiber length and seed number, a new strategy is proposed to increase cotton fiber yield by improving seed development as a whole, rather than solely focusing on manipulating fiber growth.

关键词： cotton fiber invertase leaf expansion seed abortion seed development sucrose synthase sugar signaling.

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Comparative proteomic analysis of longan (Dimocarpus longan Lour.) seed abortion

Comparative proteomic analysis of longan (Dimocarpus longan ...

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第三届全国植物蛋白质组学大会

作者： Hao Liu~1,Yan-zhen Liu~1,Shao-quan Zheng~2,Ji-mou Jiang~2,Ping Wang~3,Wei Chen~(1 * *) (1.College of Life Sciences,Fujian Agriculture and Forestry University,350002 Fuzhou 2.Fruit Research Institute,Fujian Academy of Agricultural Sciences,350013 Fuzhou, 3.College of Horticulture Sciences,Fujian Agriculture and Forestry University,50002 Fuzhou)

Two-dimensional gel electrophoresis(2-DE),coupled with mass spectroscopy,was used to study seed abortion in Dimocarpus longan Lour.(*** 64-1) by comparing normal and aborted seeds at three developmental *** than 1,000 protein spots were reproducibly detected in 2-DE gels,with 43 protein spots being significantly altered in their intensity between normal and aborted seeds at least at one ***-five proteins were identified by matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry (MALDI-TOF-MS/MS) analysis and protein database *** of the identified proteins were associated with a variety of functions,including energy and metabolism(30%), programmed cell death(9%),antioxidative processes(14%),chaperonin(23%),cell division, amino acid metabolism,secondary metabolism,and other functional ***,the expression patterns of HSP70 and cytosolic ascorbate peroxidase(cAPX) were validated by immunoblotting *** study provides a novel,global insight into proteomic differences between normal and aborted seeds in *** anticipate that identification of the differentially expressed proteins may lead to a better understanding of the molecular basis for seed abortion in longan.

关键词： Longan seed abortion Proteomics 2-DE MALDI-TOF-MS/MS

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seed loss before seed predation: experimental evidence of the negative effects of leaf feeding insects on acorn production

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Integrative Zoology 2018年第3期13卷 238-250页

作者： Tara CANELO Álvaro GAYTÁN Guillermo GONZÁLEZ-BORNAY Raul BONAL Forest Research Group INDEHESAUniversity of ExtremaduraPlasenciaSpain DITEG Research Group University of Castilla-La ManchaToledoSpain CREAF Cerdanyola del VallèsCataloniaSpain

Insect herbivory decreases plant fitness by constraining plant growth,survival and reproductive *** studies on the effects of herbivory in trees rely on correlational inter-individual comparisons and could,thus,be affected by confounding factors linked to both herbivory and plant *** the Mediterranean Holm oak(Quercus ilex)as a study model,we followed an experimental approach in which leaf-feeding insects(mainly Lepidoptera caterpillars)were excluded from some shoots in all study *** subjected to herbivore exclusion exhibited lower defoliation rates and produced more acorns than control *** constrained shoot growth throughout the study period,but had no effect on the number of female flowers produced per *** production was,however,lower in control shoots due to their higher abortion rates,and also to their greater mortality risk during summer drought,as shoots with fewer leaves were less likely to *** reaction to herbivory inhibits certain physiological pathways involved in plant growth,which,together with the effects of physical damage,reduces the amount and efficiency of the photosynthetic *** increases their vulnerability to environmental stresses,such as water deficit,which limit resource *** is likely a key factor affecting oak regeneration,as it may be a significant source of seed loss prior to pre-dispersal acorn *** experimental studies could help to elucidate its effects in contrasting *** Mediterranean regions,the harsher droughts predicted by climate change models could worsen the effects of insect herbivory on oak reproductive output.

关键词： drought stress insect defoliation oak seed abortion shoot mortality

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Morphological, physiological and molecular characteristics of the seedless 'Hongjiangcheng' sweet orange

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Horticultural Plant Journal 2023年第3期9卷 437-449页

作者： Pei Yin Wenyu Ding Haipeng Zhang Xiao Liu Hongyan Zhang Jiwu Zeng Juan Xu National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070China College of Horticulture Henan Agricultural UniversityZhengzhou 450002China Institute of Fruit Tree Research Guangdong Academy of Agricultural SciencesGuangzhou 510640China

seedless citrus has become one of the breeding goals due to its high edible ratio and convenience in fresh consumption and *** this study, the ‘Hongjiangcheng' sweet orange(WT) and its seedless mutant(MT) after cobalt-60 radiation were selected to study the formation metabolism of citrus seedless phenotype. Compared with WT, the MT had altered primary metabolite contents, as indicated by GC-MS analysis. The mature pollen of the MT was mostly distorted and shrunken, and the orange mutant exhibited significantly lower fertility than the WT. Through pollination experiments and paraffin sectioning of the MT, we observed self-compatibility during pollen tube germination in situ, in combination with the absence of natural parthenocarpy and arrested zygotic embryo development at the fourth week after pollination. From transcriptomic analyses of ovules in the fourth week, 815 differentially expressed genes(DEGs) were ***, according to the annotation of gene function and qRT-PCR analysis, Cs4g10930, Cs5g21900 and orange1.1t02243 were identified as candidate genes that may govern the mechanism of seedlessness. Finally, Agrobacterium-mediated transformation verified that the overexpression of Cs4g10930 and Cs5g21900 in Newhall navel orange calli inhibited embryoid production. This study provides a better understanding of seedless formation in citrus and two key genes that may play an important role in the early selection of seedless lines in citrus breeding programs.

关键词： Citrus sinensis seed abortion Transcriptomic sequencing Morphological aspects Candidate genes

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A Proteomic Approach for Protein Identification and Analysis in Aborted seed of Longan

A Proteomic Approach for Protein Identification and Analysis...

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中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Hao Liu1, Yan-zhen Liu1, Ji-mou Jiang2, Shao-quan Zheng2, Wei Chen1* 1College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China

＜正＞We optimized the longan seed protein extraction method and the electrophoresis conditions, and obtained the high-resolution and reproducible gel maps. At least 1000 protein spots were detected on the gels of longan

关键词： Lonagn seed abortion Proteomics Differential protein

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