Morphological, physiological and molecular characteristics of the seedless 'Hongjiangcheng' sweet orange

作     者：Pei Yin Wenyu Ding Haipeng Zhang Xiao Liu Hongyan Zhang Jiwu Zeng Juan Xu Pei Yin;Wenyu Ding;Haipeng Zhang;Xiao Liu;Hongyan Zhang;Jiwu Zeng;Juan Xu

作者机构：National Key Laboratory for Germplasm Innovation&Utilization of Horticultural CropsCollege of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070China College of HorticultureHenan Agricultural UniversityZhengzhou 450002China Institute of Fruit Tree ResearchGuangdong Academy of Agricultural SciencesGuangzhou 510640China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2023年第9卷第3期

页      面：437-449页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by the National Key Research and Development Program (Grant No. 2018YFD1000200) Special Fund for Basic Research Funds in Central Universities (Grant No. 2662018PY072) 

主　　题：Citrus sinensis Seed abortion Transcriptomic sequencing Morphological aspects Candidate genes 

摘      要：Seedless citrus has become one of the breeding goals due to its high edible ratio and convenience in fresh consumption and *** this study, the ‘Hongjiangcheng sweet orange(WT) and its seedless mutant(MT) after cobalt-60 radiation were selected to study the formation metabolism of citrus seedless phenotype. Compared with WT, the MT had altered primary metabolite contents, as indicated by GC-MS analysis. The mature pollen of the MT was mostly distorted and shrunken, and the orange mutant exhibited significantly lower fertility than the WT. Through pollination experiments and paraffin sectioning of the MT, we observed self-compatibility during pollen tube germination in situ, in combination with the absence of natural parthenocarpy and arrested zygotic embryo development at the fourth week after pollination. From transcriptomic analyses of ovules in the fourth week, 815 differentially expressed genes(DEGs) were ***, according to the annotation of gene function and qRT-PCR analysis, Cs4g10930, Cs5g21900 and orange1.1t02243 were identified as candidate genes that may govern the mechanism of seedlessness. Finally, Agrobacterium-mediated transformation verified that the overexpression of Cs4g10930 and Cs5g21900 in Newhall navel orange calli inhibited embryoid production. This study provides a better understanding of seedless formation in citrus and two key genes that may play an important role in the early selection of seedless lines in citrus breeding programs.