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Comparative quantitative analysis of fruit oil from Hippophae rhamnoides(seabuckthorn) by qNMR, FTIR and GC–MS

Chinese Herbal Medicines 2023年第4期15卷 607-613页

作者： Dattatraya Dinkar Gore Furkan Ahmad Kulbhushan Tikoo Arvind Kumar Bansal Dinesh Kumar Inder Pal Singh National Institute of Pharmaceutical Education and Research(NIPER) S.A.S.Nagar 160062India CSIR-Institute of Himalayan Bioresource and Technology Palampur 176061India

Objective: To develop a qNMR method for quantitative analysis of triacylglycerols in fruit oil of Hippophae rhamnoides(seabuckthorn, SBT) and analyze commercial samples of SBT oils using GC–MS and ***: SBT fruit oil(IPHRFH) was extracted with hexane and the triglyceride(TAG) was isolated by vacuum liquid chromatography. Six different branded SBT oils purchased from e-commerce suppliers(Amazon) and in-house prepared SBT oil was analyzed by qNMR and fatty acyl composition of TAGs determined by using NMR. In-house oil was also analysed by GC–MS and FTIR ***: The qNMR results showed that the oil contained 80.3% of triacylglycerol(TAG). The SBT oil TAGs comprised of linolenate 6.6%, palmitoleate/oleate 65.4%, and total saturated fatty acyl chain including palmitate 28% as determined by qNMR. GC–MS analysis revealed that the major acyl functionalities present in the TAG were palmitoleic acid 36.5%, oleic acid 12.9%, palmitic acid 21.2%, and linoleic acid 18%. Of the six commercial samples analyzed, samples from only one supplier(SW) were fruit oil;All others were the seed oils or mix of fruit oil and seed oil. The labels for samples except for the SW did not indicate whether it was fruit oil or seed ***: The results suggest that SBT oil should be analyzed by combination of GC–MS, FTIR and qNMR for factual content of free fatty acid or TAGs, which are chemically different in nature and affect the quality of oil. GC–MS showed the content of omega free fatty acids after hydrolysis, while qNMR and FTIR showed the content of TAGs. The major acyl functionalities found in SBT fruit oil TAGs are palmitoleate/palmitate/oleate, while linoleate and linonelate make up a minor fraction. Furthermore,analysis of commercial samples showed discrepancies between label claims and actual content.

关键词： Hippophae rhamnoides L. omega fatty acids qNMR seabuckthorn triacylglycerols

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Prediction of Landscape Pattern of Soft Sandstone Area (SSA) after seabuckthorn Planting

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American Journal of Plant Sciences 2012年第1期3卷 171-176页

作者： Jianzhong Hu 不详

As a pioneer plant in the gully slopes in the Soft Sandstone Area (SSA) for eco-economical consideration, ten years (1999-2008) planting of seabuckthorn has made 1642.83 km2, or 9.84%, of the total area of SSA change into seabuckthorn coverage. In SSA the landscape has been divided into 9 types, such as seabuckthorn, sand, water, settlement, bush, open vegetation, forest, grassland and unused land. seabuckthorn type is separated from the bush type for estimating the role of seabuckthron planting. By means of the Markov model, the developing trends of every landscape types can be determined to support the seabuckthorn project which influences the landscape pattern deeply in SSA. The prediction shows that the optimism ratio of seabuckthorn in the future should be 10.21%, the open vegetation 32.25%, and the forest percentage under 10%, which is a very wise tactics to avoid the serious death of various vegetations in SSA to match the local arid eco-environment.

关键词： Landscape Pattern Markov Model Prediction seabuckthorn Soft Sandstone Area (SSA)

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Demarcation of seabuckthorn Plantations in Three Northern Areas of China

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Forestry Studies in China 2005年第4期7卷 41-48页

作者： Hu Jian-zhong China Administration Center for Seabuckthom Development Beijing 100038 P. R. China

seabuckthorn （Hippophae rhamnoides） planting areas in the three northern areas （north, northeast and northwest） of China are divided into five planting zones： the semi-humid forest prairie climate zone for ecological and economic types of seabuckthorn plantations in the southern part of the Loess Plateau; the semi-arid steppe climate zone for similar types of plantations in the central part of the Loess Plateau; the arid desert steppe climate zone for ecological type of seabuckthorn plantations in the northern part of the Loess Plateau; the semi-arid and semi-humid steppe climate zone again for ecological and economic types of plantations in northern Hebei and western Liaoning and the cold humid steppe climate zone for economic types of plantations in the northern part of northeast China. The aim of this demarcation is to avoid a random introduction of seabuckthorn. In each of the five zones, objectives should be set and suitable seabuckthorn species, subspecies and varieties should be planted according to site conditions, seed sources and methods of tree breeding. The cultivation centers, bases, stations, or units should be established and successful models of seedling and planting methods should be encouraged. The principle of matching trees with suitable site conditions and adjusting measures to local conditions should be practiced. From a strategic viewpoint of solving ecological and economic problems of seabuckthorn development in the three northern areas, every seabuckthorn center must have its own germplasm nursery, standard plantation for popularizing, excellent seed and seedling nurseries and sufficient afforestation areas for demonstration and propaganda purposes. These measures would improve the ecological environment and promote economic and social development in the three northern areas of China.

关键词： demarcation plantation planting model seabuckthorn three northern areas

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Evaluation of nutrient value of seabuckthorn in north China

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Forestry Studies in China 2006年第1期8卷 50-52页

作者： Hu Jian-zhong Guo Xiao-feng China Administration Center for Seabuckthom Development Beijing 100038 E R. China Zhejiang Senhe Seed Co. Ltd. Hangzhou 310020 P. R. China

Five minerals or dements, crude protein, lysine, methionine＋cystine, calcium and phosphorus, were selected for evaluation of the food value of seabuckthoru （Hippophae rhamnoides ssp. sinensis） using the method of ＂food analysis＂. The decision values （δi） of seabuckthoru seeds, leaves and fruit residues rank in the fifth, sixth and ninth position among the 16 types of fodder. Seeds, leaves and fruit residues of seabuckthoru are suitable for livestock and poultry fodder. The weights of livestock and poultry are considerably increased after feeding with seabuckthoru. The development of seabuckthoru should have great potentials as food, pre-food and food additives for livestock and poultry in north China, Processing facilities should pay more attention to seabuckthoru food development methods and strategies and the seabuckthorn fodder industry should be promoted.

关键词： seabuckthorn food analysis evaluation north China

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Evaluation on Environmental Resource Value of Artificial seabuckthorn Forest in Sandstone Area during 1999-2008

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American Journal of Plant Sciences 2013年第12期4卷 2314-2317页

作者： Jianzhong Hu China National Administration Center for seabuckthorn Development Beijing China

China seabuckthorn Ecological Project has been implemented for a decade (from 1999 to 2008) with an increasing seabuckthorn area of 1642.83 km2 in?the Sandstone Areas of Shanxi, Shaanxi and Inner Mongolia—the most serious soil erosion area in China. The vast artificial seabuckthorn forest’s value in environmental resource is assessed via 7 indicators on first-level, 15 indicators on secondary level and 35 indicators on third level in this research. As of 2008, the seabuckthorn environmental resource value is 10.017 billion RMB Yuan, which equals to 37.1 times of the project’s total investment of 0.27 billion RMB Yuan. It is proposed in this research that the external economical values such as soil and water conservation, windbreak and sand-fixation can be realized through relative national policies and taxes. Carbon-fixation and oxygen-producing value can be achieved through the international carbon trade.

关键词： seabuckthorn Ecological Project Environmental Resource Value Evaluation Sandstone Areas China

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Assessing Anthropogenic Pressure and Its Impact on Hippophae salicifolia Pockets in Central Himalaya,Uttarakhand

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Journal of Mountain Science 2013年第3期10卷 464-471页

作者： Deepak DHYANI Shalini DHYANI RK MAIKHURI G.B.Pant Institute of Himalayan Environment and Development Garhwal UnitSrinagar GarhwalUttarakhandIndia

Natural habitat ofHippophae salicifolia in Central Himalaya is continuously being degraded due to habitat destruction and harvesting. Although logging is prohibited, habitat destruction has increased because of regular road construction, repairing and broadening activities. In addition, Hippophae resources are continuously being harvested by lopping （both partial and complete） for fuelwood, fodder and fruits in higher Himalayan region. This paper presents a detailed analysis of relationship between density, demographic structure, and harvesting of H. salicifolia growing pockets in the five major valleys （Gangotri, Yamunotri, Niti, Mana and Bhyundhar） of Uttarakhand in Central Himalaya, India. A total of 12o quadrats were laid randomly to study population structure, regeneration, sex ratio and lopping using quadrats of lOO m2 （a4 in each valley） in Hippophae growing patches. Our study shows that the density, size distribution, and regeneration of Hippophae vary considerably among the major valleys. Trees in the Yamunotri valley have the highest density of large trees but the lowest density of seedlings. In contrast, there are few large trees but many seedlings in the Mana valley. The number and size of lopped trees also varied among the valleys. Lopping was greatest in Bhyundhar （11.4%） and Yamunotri （19.7%） and least in Niti （3.9%）. The size of lopped trees differed substantially as well. In Bhyundhar, the largest trees were taken while saplings were taken in Yamunotri. Our study revealed that unsustainable harvesting from plants for fuel, fencing and fruits along with road broadening activities in Central Himalaya are the main cause ofhabitat destruction. Our research highlights the urgent need for in-situ and ex-situ conservation of Hippophae salicifolia so that it＇s potential can be harnessed sustainably by rural hill societies for their socio-economic development.

关键词： Central Himalaya seabuckthorn Dioecious Disturbance Habitat Conservation

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Pisha sandstone: Causes, processes and erosion options for its control and prospects

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International Soil and Water Conservation Research 2019年第1期7卷 1-8页

作者： Zhishui Liang Zhiren Wu Wenyi Yao Mohammad Noori Caiqian Yang Peiqing Xiao Yuanbao Leng Lin Deng School of Civil Engineering Southeast UniversityNanjing 210096China School of the Environment and Safety Engineering Jiangsu UniversityZhenjiang 212013China Key Laboratory of Soil and Water Loess Process and Control on Loess Plateau of MWR Yellow River Institute of Hydraulic Research45 Shunhe RoadZhengzhou 450003China International Institute for Urban Systems Engineering Southeast UniversityNanjing 210096China

Pisha sandstone is a special kind of soft rock in the semiarid region along the up and middle reaches of Yellow River and Loess Plateau and is severely eroded by wind,rainfall and gravity *** sandstone region is called 'the most severe water loss and soil erosion in the world' and referred to as the 'Earth Cancer' by local residents and experts both at home and *** plan for the control of Pisha sandstone started in the *** then a series of projects have been implemented,both engineering and vegetation measures have been developed and widely used to protect Pisha sandstone from *** seabuckthom was introduced as a viable approach for soil erosion protection and its implementation resulted in a breakthrough to control Loess Plateau,it was considered as an important and efficient *** the seabuckthom flexible dam was proposed for coarse sediment retention and fine sediment *** then a new comprehensive control model using consolidation materials combined with vegetation was proposed and promising results were achieved both in laboratory and field *** the future,the complex erosion mechanism and ecological carrying capacity should he studied further and more efficient and practical comprehensive measures shall be ***,monitoring systems shall be used to predict and detect the changes in the Pisha sandstone slope and structure.

关键词： Water loss and soil erosion Land degradation Ecological restoration seabuckthorn Comprehensive measure

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Comparative antitumor and anti-proliferative activities of Hippophae rhamnoides L. leaves extracts

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Journal of Coastal Life Medicine 2015年第3期3卷 228-232页

作者： Javid Ali Bashir Ahmad PCSIR Laboratories Complex Jamrude Road PeshawarKPK-Pakistan Center of Biotechnology and Microbiology University of PeshawarKPK-Pakistan

Objective:To evaluate the antitumor and anti-proliferative activities of methanol,aqueous,acetone,ethyl acetate,ethanol,chloroform and n-hexane extracts of Hippophae rhamnoides ***:Antitumor activities were evaluated by using the antitumor potato disc assay by using inoculums(Agrobacterium tumefaciens)with three different concentrations of test samples(10,100 and 1000 mg/L).Anti-proliferative activity was evaluated by the given method of methyl thiazolyl tetrazolium *** concentrations of the extract ranging from 0.039 to 10 mg/mL were tested against HeLa ***:Highest tumors inhibition activity(60.9%and 55.8%)was shown by methanol and ethanol extracts,with EC_(50) values of 424.41 and 434.61 mg/L *** 10 mg/mL,The highest cell inhibition 75.61%was observed in methanol extract and the lowest 36.59%were calculated in n-hexane *** difference in tumor and cell inhibition(%)may be due to the different concentration of active compounds responsible for antitumor and anti-proliferative *** extracts have considerable level of tumor and cell inhibitiory effect in a dose dependent ***:Our finding showed that Hippophae rhamnoides leaves are a potent natural source of antitumor and antiproliferative agent.

关键词： seabuckthorn Solvent extracts Tumor inhibition Anti-cancer Potato disc assay HeLa cell line

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Proximate composition,mineral contents,phytochemical constituents,antimicrobial activities and Fourier transforms infrared spectroscopy analysis of bark,stem and seed of Hippophae rhamnoides Linn

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Journal of Coastal Life Medicine 2015年第6期 486-490页

作者： Javid Ali Bashir Ahmad Said Hassan Muhammad Siddique Farrah Gul Shafaat Ullah Center of Biotechnology and Microbiology University of PeshawarKPKPakistan PCSIR Laboratories Complex Jamrud Road PeshawarKPK-Pakistan

Objective:To compare the proximate composition,mineral contents,antimicrobial,phytochemical and Fourier transforms infrared(FTIR)spectroscopy analysis of bark,stem and seed of Hippophae ***:Proximate composition was determined according to the described *** analysis was carried out by atomic absorption spectroscopy and flame *** activities were evaluated according to the agar well diffusion *** qualitative analysis was carried out according to the described methods and functional groups were determined by FTIR Prestige-21 Shimadzu ***:The proximate analysis showed high content of protein and fiber in stem and *** content of Na(900 mg/L)and K(670 mg/L)was found in bark powder,while in seed,high contents of Ca(800 mg/L),Mg(725 mg/L),Fe(250 mg/L)Zn(90 mg/L)and Mn(65 mg/L)were found compared to stem and ***,flavonoids and tannins showed high contents in stem and bark of all *** bark aqueous extract showed high zone of inhibition against Staphylococcus aureus(21 mm)and Escherichia coli(20 mm),while methanol extract of stem showed high zone of inhibition(14 mm and 13 mm)against Enterococcus faecalis and Escherichia coli *** aqueous extract of bark documented high zone of inhibition against Aspergillus niger(21 mm)and Aspergillus parasiticus(20 mm).FTIR spectra revealed the presence of OH,C-O and C=O functional ***:The study concludes that bark,stem and seed extracts will be useful guideline for the new syntheses of feed,food supplements and herb drugs with various combination,which can be used for the treatment of many diseases at global level especially in tropical regions as well as the male nutrition problems in these areas.

关键词： seabuckthorn Proximate composition Minerals Antibacterial Antifungal FTIR spectra

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Evaluation of antioxidant activities of Hippophae rhamnoides Linn leaves extracts

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Journal of Coastal Life Medicine 2015年第5期 379-381页

作者： Javid Ali Bashir Ahmad Center of Biotechnology and Microbiology University of PeshawarKPKPakistan PCSIR Laboratories Complex Jamrude Road Peshawar KPKPakistan

Objective:To investigate the antioxidant activity of aqueous,methanol,ethanol,acetone,ethyl acetate,chloroform and n-hexane extracts of Hippophae rhamnoides(***)***:Antioxidant activity was evaluated by using in-vitro antioxidant assays model 1,1’-diphenyl-2-picrylhydrazyl radical-scavenging *** antioxidant activities were compared with standard antioxidant agents such as ascorbic ***:The antioxidant activities(%inhibition)of all the tested extracts were increased in the order ***>ethanol>aqueous>acetone>chloroform>ethyl acetate>*** methanol extract EC_(50)(μg/mL)value was compatible with vitamin C(standard).The antioxidant activity of *** leaves extracts increased in a dose dependent ***:It was observed that *** was a potential resource of antioxidants and thus could put off numerous radical linked diseases.

关键词： seabuckthorn Solvent extracts Free radical scavenging activities Vitamin C EC_(50)

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